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Protein

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase

Gene

PAPS

Organism
Vaccinia virus (strain Ankara) (VACV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Displays methyltransferase, positive regulation of the poly(A) polymerase and transcription elongation activities. Involved in the modification of both mRNA ends and in intermediate and late gene positive transcription elongation. At the mRNAs 5' end, methylates the ribose 2' OH group of the first transcribed nucleotide, thereby producing a 2'-O-methylpurine cap. At the 3' end, functions as a processivity factor which stimulates the activity of the viral poly(A) polymerase VP55 that creates mRNA's poly(A) tail. In the presence of VP39, VP55 does not dissociate from the RNA allowing tail elongation to around 250 adenylates.By similarity

Catalytic activityi

S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA].

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22mRNA capPROSITE-ProRule annotation1
Binding sitei39S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei66S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei68S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei72S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei95S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei97S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei116S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei138S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei175For methyltransferase activityPROSITE-ProRule annotation1
Binding sitei182mRNA capPROSITE-ProRule annotation1
Binding sitei233mRNA capPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionElongation factor, Methyltransferase, Transferase
Biological processmRNA capping, mRNA processing, Protein biosynthesis, Transcription
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase (EC:2.1.1.57)
Alternative name(s):
Poly(A) polymerase regulatory subunit
Poly(A) polymerase small subunit
Short name:
PAP-S
VP39
Gene namesi
Name:PAPS
Ordered Locus Names:MVA087R, ACAM3000_MVA_087
OrganismiVaccinia virus (strain Ankara) (VACV)
Taxonomic identifieri126794 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000172909 Componenti: Genome
  • UP000159908 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000991101 – 333Cap-specific mRNA (nucleoside-2'-O-)-methyltransferaseAdd BLAST333

Interactioni

Subunit structurei

Methyltransferase activity: Monomer, poly(A) polymerase activity: Heterodimer composed of a catalytic component, VP55, and a processivity factor, VP39. Interacts with Rap94 and NPH-I; these interactions might help linking transcription to capping and polyadenylation.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ76ZT1.
SMRiQ76ZT1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni169 – 333Binding to Rap94By similarityAdd BLAST165
Regioni169 – 249Binding to NPH-IPROSITE-ProRule annotationAdd BLAST81
Regioni177 – 180mRNA cap bindingPROSITE-ProRule annotation4
Regioni205 – 207mRNA cap bindingPROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Poxvirus/kinetoplastid 2'-O-MTase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR000176. mRNA_MeTrfase-like.
IPR025804. Pox/kineto_cap_MeTfrase.
IPR030375. Poxvir_cap_MeTfrase.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF01358. PARP_regulatory. 1 hit.
PIRSFiPIRSF003726. PolA_polym_reg_poxV. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiView protein in PROSITE
PS51612. SAM_MT_2O_PK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q76ZT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVVSLDKPF MYFEEIDNEL DYEPESANEV AKKLPYQGQL KLLLGELFFL
60 70 80 90 100
SKLQRHGILD GATVVYIGSA PGTHIRYLRD HFYNLGVIIK WMLIDGRHHD
110 120 130 140 150
PILNGLRDVT LVTRFVDEEY LRSIKKQLHP SKIILISDVR SKRGGNEPST
160 170 180 190 200
ADLLSNYALQ NVMISILNPV ASSLKWRCPF PDQWIKDFYI PHGNKMLQPF
210 220 230 240 250
APSYSAEMRL LSIYTGENMR LTRVTKSDAV NYEKKMYYLN KIVRNKVVVN
260 270 280 290 300
FDYPNQEYDY FHMYFMLRTV YCNKTFPTTK AKVLFLQQSI FRFLNIPTTS
310 320 330
TEKVSHEPIQ RKISSKNSMS KNRNSKRSVR SNK
Length:333
Mass (Da):38,888
Last modified:July 5, 2004 - v1
Checksum:i55A299DA4AAE1AD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94848 Genomic DNA. Translation: AAB96504.1.
AY603355 Genomic DNA. Translation: AAT10485.1.
RefSeqiYP_232977.1. NC_006998.1.

Genome annotation databases

GeneIDi3707551.
KEGGivg:3707551.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMCE_VACCA
AccessioniPrimary (citable) accession number: Q76ZT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 5, 2004
Last modified: May 10, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families