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Q76ZS7 (TOP1_VACCA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1B

Short name=TopIB
EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Late protein H6
Gene names
Name:TOP1
Ordered Locus Names:MVA096R, ACAM3000_MVA_096
OrganismVaccinia virus (strain Ankara) (VACV)
Taxonomic identifier126794 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Induction

Expressed in the late phase of the viral replicative cycle.

Sequence similarities

Belongs to the type IB topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314DNA topoisomerase 1B
PRO_0000145214

Sites

Active site2741O-(3'-phospho-DNA)-tyrosine intermediate By similarity
Site1681Involved in religation By similarity

Sequences

Sequence LengthMass (Da)Tools
Q76ZS7 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: E76FBCFCBF259D27

FASTA31436,666
        10         20         30         40         50         60 
MRALFYKDGK LFTDNNFLNP VSDDNPAYEV LQHVKIPTHL TDVVVYEQTW EEALTRLIFV 

        70         80         90        100        110        120 
GSDSKGRRQY FYGKMHVQNR NAKRDRIFVR VYNVMKRINC FINKNIKKSS TDSNYQLAVF 

       130        140        150        160        170        180 
MLMETMFFIR FGKMKYLKEN ETVGLLTLKN KHIEISPDEI VIKFVGKDKV SHEFVVHKSN 

       190        200        210        220        230        240 
RLYKPLLKLT DDSSPEEFLF NKLSERKVYE CIKQFGIRIK DLRTYGVNYT FLYNFWTNVK 

       250        260        270        280        290        300 
SISPLPSPKK LIALTIKQTA EVVGHTPSIS KRAYMATTIL EMVKDKNFLD VVSKTTFDEF 

       310 
LSIVVDHVKS STDG 

« Hide

References

[1]"The complete genomic sequence of the modified vaccinia Ankara strain: comparison with other orthopoxviruses."
Antoine G., Scheiflinger F., Dorner F., Falkner F.G.
Virology 244:365-396(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J., Khristova M., Wohlhueter R.M.
Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate Acambis 3000.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U94848 Genomic DNA. Translation: AAB96509.1.
AY603355 Genomic DNA. Translation: AAT10494.1.
RefSeqYP_232986.1. NC_006998.1.

3D structure databases

ProteinModelPortalQ76ZS7.
SMRQ76ZS7. Positions 1-314.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3707560.

Phylogenomic databases

ProtClustDBPHA3101.

Family and domain databases

Gene3D1.20.120.380. 1 hit.
3.30.66.10. 1 hit.
3.90.15.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR027362. TopoI_cat_dom2.
IPR013500. TopoI_cat_euk.
IPR015346. TopoI_N_vir.
[Graphical view]
PfamPF01028. Topoisom_I. 1 hit.
PF09266. VirDNA-topo-I_N. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SUPFAMSSF56349. SSF56349. 1 hit.
PROSITEPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_VACCA
AccessionPrimary (citable) accession number: Q76ZS7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 5, 2004
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families