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Q76WK3 (VP7_ROTHK) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid glycoprotein VP7
OrganismRotavirus A (strain Human/Japan/KU/1995 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) [Complete proteome]
Taxonomic identifier10952 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved By similarity.

Subunit structure

Homotrimer; in the presence of calcium By similarity. Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 By similarity.

Subcellular location

Virion By similarity. Host endoplasmic reticulum lumen Potential. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

Post-translational modification

The N-terminus is blocked possibly by pyroglutamic acid By similarity.

N-glycosylated By similarity.

Intramolecular disulfide bonds By similarity.

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.

Sequence similarities

Belongs to the rotavirus VP7 family.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: Q76WK3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q76WK3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.
Note: Produced by alternative initiation at Met-30 of isoform 1. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5050 Potential
Chain51 – 326276Outer capsid glycoprotein VP7 Potential
PRO_0000369113

Regions

Compositional bias240 – 2456Poly-Thr

Amino acid modifications

Glycosylation691N-linked (GlcNAc...); by host Potential
Glycosylation2381N-linked (GlcNAc...); by host Potential

Natural variations

Alternative sequence1 – 2929Missing in isoform 2.
VSP_038621

Experimental info

Sequence conflict1491E → V in BAE78582. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: F403990A04C6D7C8

FASTA32637,344
        10         20         30         40         50         60 
MYGIEYTTIL IFLISIILLN YILKSVTRIM DYIIYRFLLI TVALFALTRA QNYGLNLPIT 

        70         80         90        100        110        120 
GSMDTVYTNS TQEEVFLTST LCLYYPTEAS TQINDGDWKD SLSQMFLTKG WPTGSVYFKE 

       130        140        150        160        170        180 
YSSIVDFSVD PQLYCDYNLV LMKYDQSLEL DMSELADLIL NEWLCNPMDI TLYYYQQSGE 

       190        200        210        220        230        240 
SNKWISMGSS CTVKVCPLNT QTLGIGCQTT NVDSFEMVAE NEKLAIVDVV DGINHKINLT 

       250        260        270        280        290        300 
TTTCTIRNCK KLGPRENVAV IQVGGSNVLD ITADPTTNPQ TERMMRVNWK KWWQVFYTIV 

       310        320 
DYINQIVQVM SKRSRSLNSA AFYYRV 

« Hide

Isoform 2 [UniParc].

Checksum: 10CF913F472A3343
Show »

FASTA29733,944

References

[1]"Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies."
Taniguchi K., Hoshino Y., Nishikawa K., Green K.Y., Maloy W.L., Morita Y., Urasawa S., Kapikian A.Z., Chanock R.M., Gorziglia M.
J. Virol. 62:1870-1874(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"High-resolution molecular and antigen structure of the VP8* core of a sialic acid-independent human rotavirus strain."
Monnier N., Higo-Moriguchi K., Sun Z.Y., Prasad B.V.V., Taniguchi K., Dormitzer P.R.
J. Virol. 80:1513-1523(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16343 mRNA. Translation: BAA03847.1.
AB222787 Genomic RNA. Translation: BAE78581.1.
AB222788 Genomic RNA. Translation: BAE78582.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001963. VP7.
[Graphical view]
PfamPF00434. VP7. 1 hit.
[Graphical view]
ProDomPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameVP7_ROTHK
AccessionPrimary (citable) accession number: Q76WK3
Secondary accession number(s): Q2MH33, Q2MH34
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 5, 2004
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families