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Protein

Matrix protein 1

Gene

M

Organism
Influenza A virus (strain A/Swine/29/1937 H1N1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place.UniRule annotation
Determines the virion's shape: spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms.UniRule annotation

Miscellaneous

Most abundant protein in virion. When expressed alone can form virus-like particles in transfected cells.UniRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix protein 1UniRule annotation
Short name:
M1UniRule annotation
Gene namesi
Name:MUniRule annotation
OrganismiInfluenza A virus (strain A/Swine/29/1937 H1N1)
Taxonomic identifieri382842 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

  • Virion membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003262761 – 252Matrix protein 1Add BLAST252

Interactioni

Subunit structurei

Homodimer and homomultimer. Interacts with NEP. Binds ribonucleocapsid by both interacting with genomic RNA and NP protein. May interact with HA and NA. Cannot bind NP without genomic RNA.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ76V10.
SMRiQ76V10.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 164Membrane-bindingUniRule annotationAdd BLAST164
Regioni165 – 252RNP-bindingUniRule annotationAdd BLAST88

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi101 – 105Nuclear localization signalUniRule annotation5

Sequence similaritiesi

Belongs to the influenza viruses Matrix protein M1 family.UniRule annotation

Family and domain databases

Gene3Di1.10.10.180. 1 hit.
HAMAPiMF_04068. INFV_M1. 1 hit.
InterProiView protein in InterPro
IPR013188. Flu_matrix_M1_C.
IPR001561. Flu_matrix_M1_N.
IPR015799. Flu_matrix_M1_N_sub2.
PfamiView protein in Pfam
PF00598. Flu_M1. 1 hit.
PF08289. Flu_M1_C. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD596253. Flu_matrix_M1_C. 1 hit.
PD001061. Flu_matrix_M1_N. 1 hit.
SMARTiView protein in SMART
SM00759. Flu_M1_C. 1 hit.
SUPFAMiSSF48145. SSF48145. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Only the first 9 residues are shared by the 2 isoforms.
Isoform M1 (identifier: Q76V10-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLTEVETY VLSIVPSGPL KAEIAQRLED VFAGKNTDLE ALMEWLKTRP
60 70 80 90 100
ILSPLTKGIL GFVFTLTVPS ERGLQRRRFV QNALNGNGDP NNMDKAVKLY
110 120 130 140 150
RKLKREITFH GAKEVALSYS AGALASCMGL IYNRMGTVTT EVAFGLVCAT
160 170 180 190 200
CEQIADSQHR SHRQMVTTTN PLIRHENRMV LASTTAKAME QMAGSSEQAA
210 220 230 240 250
EAMEVASQAR QMVQAMRTIG THPSSSAGLK DDLLENLQAY QKRMGVQMQR

FK
Length:252
Mass (Da):27,852
Last modified:July 5, 2004 - v1
Checksum:i70B8DB1C17653A9A
GO
Isoform M2 (identifier: Q67206-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q67206.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:97
Mass (Da):11,183
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63517 Genomic RNA. Translation: AAA43342.1.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiM1_I37A0
AccessioniPrimary (citable) accession number: Q76V10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: August 30, 2017
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families