Reviewed, UniProtKB/Swiss-Prot Q76NV5 (HPPD_DICDI)

Names and origin

Protein names

Recommended name:
    4-hydroxyphenylpyruvate dioxygenase
    EC=1.13.11.27
Alternative name(s):
    4-hydroxyphenylpyruvic acid oxidase
      Short name=HPPDase
      Short name=4HPPD
      Short name=HPD

Gene names

Name:	hpd
ORF Names:	DDB_G0277511

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	4-hydroxyphenylpyruvate + O2 = homogentisate + CO2.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 3/6.
Sequence similarities	Belongs to the 4HPPD family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Coding sequence diversity	Alternative splicing
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-hydroxyphenylpyruvate dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q76NV5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q76NV5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MEM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	4-hydroxyphenylpyruvate dioxygenase		PRO_0000327996
Sites
	Metal binding	169	1	Iron By similarity
	Metal binding	252	1	Iron By similarity
	Metal binding	335	1	Iron By similarity
Natural variations
	Alternative sequence	1	1	M → MEM in isoform 2.		VSP_032743

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EC5A1D87AB196355	FASTA	367	41,738
	10 20 30 40 50 60 MEGFDHVTFW VGNALQAATY YIARFGFQNL AYSGLETGNR QFATHVIHQN NIIMAFTSPL 70 80 90 100 110 120 TGDNKDYADH MMRHGDGVKD IAFNVKDVQH IYDEAVKAGA QSVKEPHQIK DEHGIVTLAT 130 140 150 160 170 180 IMSPYGETTH TFVDRSQYKG AFLPGFTYKV ASDPLSNITE PVGLNLIDHV VSNHADKMME 190 200 210 220 230 240 PVVQWYEKVL QFHRFWSVDD KTIHTEYSSL RSVVVADKSE KVKLPINEPA NGIRKSQIQE 250 260 270 280 290 300 YVDFYNGAGV QHIALKTDNI IDAISKLRSR GVSFLTVPKT YYTSLREKLQ HSSLEIKEDL 310 320 330 340 350 360 DTLEKLHILI DYDDKGYLLQ IFTNNVEDKP TVFFEIIQRN NHDGFGAGNF KSLFEAIERQ QETRGNL
	Isoform 2. Checksum: DCF4609BD8926DF9	FASTA	369	41,998
	10 20 30 40 50 60 MEMEGFDHVT FWVGNALQAA TYYIARFGFQ NLAYSGLETG NRQFATHVIH QNNIIMAFTS 70 80 90 100 110 120 PLTGDNKDYA DHMMRHGDGV KDIAFNVKDV QHIYDEAVKA GAQSVKEPHQ IKDEHGIVTL 130 140 150 160 170 180 ATIMSPYGET THTFVDRSQY KGAFLPGFTY KVASDPLSNI TEPVGLNLID HVVSNHADKM 190 200 210 220 230 240 MEPVVQWYEK VLQFHRFWSV DDKTIHTEYS SLRSVVVADK SEKVKLPINE PANGIRKSQI 250 260 270 280 290 300 QEYVDFYNGA GVQHIALKTD NIIDAISKLR SRGVSFLTVP KTYYTSLREK LQHSSLEIKE 310 320 330 340 350 360 DLDTLEKLHI LIDYDDKGYL LQIFTNNVED KPTVFFEIIQ RNNHDGFGAG NFKSLFEAIE RQQETRGNL

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: AX4.
[3]	"Identification of novel centrosomal proteins in Dictyostelium discoideum by comparative proteomic approaches." Reinders Y., Schulz I., Graef R., Sickmann A. J. Proteome Res. 5:589-598(2006) [PubMed: 16512674] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC117081 Genomic DNA. Translation: AAS38823.1. AAFI02000020 Genomic DNA. Translation: EAL68718.1. AAFI02000020 Genomic DNA. Translation: EAS66903.1.
RefSeq	XP_001134587.1. XP_642615.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3393771.
KEGG	ddi:DDB_0231604.
Organism-specific databases
dictyBase	DDB_G0277511. hpd.
Phylogenomic databases
OMA	Q76NV5. VQDRPTV.
Enzyme and pathway databases
BRENDA	1.13.11.27. 424.
Family and domain databases
InterPro	IPR005956. 4OHPhenylPyrv_dOase. IPR004360. Glyas_bleo-R_dOase. [Graphical view]
PANTHER	PTHR11959. HPP_dOase. 1 hit.
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
PIRSF	PIRSF009283. HPP_dOase. 1 hit.
TIGRFAMs	TIGR01263. 4HPPD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPD_DICDI

Accession

Primary (citable) accession number: Q76NV5
Secondary accession number(s): Q1ZXJ9, Q54ZM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)