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Reviewed, UniProtKB/Swiss-Prot Q76N33 (STALP_MOUSE)

Last modified November 3, 2009. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    AMSH-like protease
      Short name=AMSH-LP
    EC=3.1.2.15
Alternative name(s):
    AMSH family protein
      Short name=AMSH-FP
    STAM-binding protein-like 1
Gene names
Name: Stambpl1
Synonyms: Amshlp
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains By similarity.

Catalytic activity

Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Tissue specificity

Ubiquitously expressed. Isoform 1 is widely expressed while isoform 2 is testis-specific. Ref.1

Domain

The JAMM motif is essential for the protease activity By similarity.

Sequence similarities

Belongs to the peptidase M67C family.

Contains 1 MPN (JAB/Mov34) domain.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q76N33-1)

Also known as: AMSH-FPalpha; ALM alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q76N33-2)

Also known as: AMSH-FPbeta; ALM beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-166: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 436436AMSH-like protease
PRO_0000194875

Regions

Domain264 – 373110MPN
Motif347 – 36014JAMM motif

Sites

Metal binding3471Zinc 1; catalytic By similarity
Metal binding3491Zinc 1; catalytic By similarity
Metal binding3601Zinc 1; catalytic By similarity
Metal binding3621Zinc 2 By similarity
Metal binding4021Zinc 2 By similarity
Metal binding4081Zinc 2 By similarity
Metal binding4101Zinc 2 By similarity
Site2921Indirect Zinc-binding By similarity

Amino acid modifications

Modified residue251Phosphoserine By similarity
Modified residue2421Phosphoserine By similarity

Natural variations

Alternative sequence1 – 166166Missing in isoform 2.
VSP_014649

Experimental info

Sequence conflict1941I → M in AAH18343. Ref.3
Sequence conflict2011V → M in AAH18343. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (AMSH-FPalpha) (ALM alpha) [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 6AF263DF5EC4FD9B

FASTA43649,640
        10         20         30         40         50         60 
MEQPFTVNSL KKLAAMPDHT DVSLSPEERV RALSKLGCNI SINEDITPRR YFRSGVEMER 

        70         80         90        100        110        120 
MASVYLEEGN LENAFVLYNK FITLFVEKLP SHRDYQQCAV PEKQDIMKKL KEIAFPRTDE 

       130        140        150        160        170        180 
LKTDLLRKYN IEYQEYLQSK NKYKAEILKK LEHQRLIEAE RQRIAQMRQQ QLESEQFLFF 

       190        200        210        220        230        240 
EDQLKKQELA RGQIRGQDSP VLSEQTDGSA LSCFSTHQSN SLRNAFADHP HKSDGSNFAN 

       250        260        270        280        290        300 
YSPPVNRALK PAATLSAVQN LVVEGLRCVV LSRDLCHKFL LLADSNTVRG IETCGILCGK 

       310        320        330        340        350        360 
LTHNEFTITH VVVPKQSAGP DYCDVENVEE LFNVQDQHGL LTLGWIHTHP TQTAFLSSVD 

       370        380        390        400        410        420 
LHTHCSYQLM LPEAIAIVCS PKHKDTGIFR LTNAGMLEVS TCKKKGFHPH TKDPKLFSIC 

       430 
SHVLVKDIKT TVLDLR

« Hide

Isoform 2 (AMSH-FPbeta) (ALM beta).

Checksum: 1540CECE3AEDD696
Show »

FASTA27030,014

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References

« Hide 'large scale' references
[1]"A newly identified AMSH-family protein is specifically expressed in haploid stages of testicular germ cells."
Kitajima K., Matsumoto K., Tahara M., Takahashi H., Nakamura T., Nakamura T.
Biochem. Biophys. Res. Commun. 309:135-142(2003) [PubMed: 12943674] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
Tissue: Brain.
[2]"Cloning of a gene for the mouse AMSH-like-protein."
Ishii N., Sugamura K.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Czech II.
Tissue: Mammary gland.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AB066211 mRNA. Translation: BAD00166.1.
AB066212 mRNA. Translation: BAD00167.1.
AB066213 mRNA. Translation: BAD00168.1.
AB010121 mRNA. Translation: BAD06408.1.
AB010122 mRNA. Translation: BAD06409.1.
BC018343 mRNA. Translation: AAH18343.1. Different initiation.
IPIIPI00312188.
IPI00608113.
RefSeqNP_083958.3.
UniGeneMm.130952

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ76N33.

Protein family/group databases

MEROPSM67.003.

PTM databases

PhosphoSiteQ76N33.

Proteomic databases

PRIDEQ76N33.

Genome annotation databases

EnsemblENSMUST00000054956; ENSMUSP00000059927; ENSMUSG00000024776; Mus musculus. [Genome view]
ENSMUST00000119603; ENSMUSP00000112938; ENSMUSG00000024776; Mus musculus. [Genome view]
GeneID76630.
KEGGmmu:76630.
UCSCuc008hgc.1. mouse.
uc008hge.1. mouse.

Organism-specific databases

CTD76630.
MGIMGI:1923880. Stambpl1.

Phylogenomic databases

HOGENOMQ76N33.
HOVERGENQ76N33.
OMAFTVNSLK.

Enzyme and pathway databases

BRENDA3.1.2.15. 244.

Gene expression databases

ArrayExpressQ76N33.
BgeeQ76N33.
CleanExMM_STAMBPL1.
GenevestigatorQ76N33.
GermOnlineENSMUSG00000024776. Mus musculus.

Family and domain databases

InterProIPR000555. Mov34_MPN_PAD1.
[Graphical view]
PfamPF01398. Mov34. 1 hit.
[Graphical view]
SMARTSM00232. JAB_MPN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio345496.
SOURCESearch...

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Entry information

Entry nameSTALP_MOUSE
AccessionPrimary (citable) accession number: Q76N33
Secondary accession number(s): Q76LY0, Q8VEK5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 5, 2004
Last modified: November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents