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Protein

Centrosomal protein of 68 kDa

Gene

CEP68

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maintenance of centrosome cohesion, probably as part of a linker structure which prevents centrosome splitting (PubMed:18042621). Required for localization of CDK5RAP2 to the centrosome during interphase (PubMed:24554434, PubMed:25503564).3 Publications

GO - Molecular functioni

  • protein domain specific binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

  • centriole-centriole cohesion Source: UniProtKB
  • centrosome cycle Source: UniProtKB
  • protein localization to organelle Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 68 kDa
Short name:
Cep68
Gene namesi
Name:CEP68
Synonyms:KIAA0582
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000011523.13.
HGNCiHGNC:29076. CEP68.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi331 – 337Missing : Prevents binding to BTRC and down-regulation of CEP68 during mitosis. 1 Publication7
Mutagenesisi332S → A: Prevents binding to BTRC and down-regulation of CEP68 during mitosis. 1 Publication1
Mutagenesisi337D → A: Reduces CEP68 binding to BTRC. 1 Publication1

Organism-specific databases

DisGeNETi23177.
OpenTargetsiENSG00000011523.
PharmGKBiPA134991391.

Polymorphism and mutation databases

BioMutaiCEP68.
DMDMi62899863.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000894941 – 757Centrosomal protein of 68 kDaAdd BLAST757

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei332Phosphoserine; by PLK11 Publication1
Modified residuei472PhosphoserineBy similarity1
Modified residuei478PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation by PLK1 is required for binding to BTRC in prometaphase (PubMed:25503564). Phosphorylated directly or indirectly by NEK2 (PubMed:24554434). NEK2-mediated phosphorylation promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143).3 Publications
Ubiquitinated and targeted for proteasomal degradation in early mitosis by the SCF(BTRC) and/or SCF(FBXW11) E3 ubiquitin-protein ligase complexes (PubMed:25704143, PubMed:25503564). Degradation is complete by prometaphase and is required for removal of CDK5RAP2 from the peripheral pericentriolar material and subsequent centriole separation (PubMed:25503564).2 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ76N32.
MaxQBiQ76N32.
PaxDbiQ76N32.
PeptideAtlasiQ76N32.
PRIDEiQ76N32.

PTM databases

iPTMnetiQ76N32.
PhosphoSitePlusiQ76N32.

Expressioni

Gene expression databases

BgeeiENSG00000011523.
CleanExiHS_CEP68.
ExpressionAtlasiQ76N32. baseline and differential.
GenevisibleiQ76N32. HS.

Organism-specific databases

HPAiHPA040493.
HPA040620.

Interactioni

Subunit structurei

Interacts with CNTLN; the interaction recruits CEP68 to the centrosome (PubMed:24554434). Interacts with the SCF(FBXW11) complex which contains SKP1, CUL1 and FBXW11; the interaction is probably mediated by FBXW11 and the complex also contains CDK5RAP2 and PCNT (PubMed:25503564). Also interacts with F-box protein BTRC (PubMed:25704143, PubMed:25503564). Interacts with serine/threonine-protein kinase PLK1; the interaction leads to phosphorylation of CEP68 and its subsequent degradation (PubMed:25503564). Interacts with NEK2; the interaction leads to phosphorylation of CEP68 (PubMed:24554434).4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116789. 12 interactors.
DIPiDIP-57845N.
IntActiQ76N32. 19 interactors.
MINTiMINT-7034577.
STRINGi9606.ENSP00000367229.

Structurei

3D structure databases

ProteinModelPortaliQ76N32.
SMRiQ76N32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi532 – 537Poly-Ser6

Phylogenomic databases

eggNOGiENOG410IIDK. Eukaryota.
ENOG41129RE. LUCA.
GeneTreeiENSGT00810000125473.
HOVERGENiHBG050898.
InParanoidiQ76N32.
KOiK16764.
OMAiQAEYWAC.
OrthoDBiEOG091G0T7P.
PhylomeDBiQ76N32.
TreeFamiTF333570.

Family and domain databases

InterProiView protein in InterPro
IPR026696. AKAP6/CEP68.
PANTHERiPTHR14514. PTHR14514. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q76N32-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALGEEKAEA EASEDTKAQS YGRGSCRERE LDIPGPMSGE QPPRLEAEGG
60 70 80 90 100
LISPVWGAEG IPAPTCWIGT DPGGPSRAHQ PQASDANREP VAERSEPALS
110 120 130 140 150
GLPPATMGSG DLLLSGESQV EKTKLSSSEE FPQTLSLPRT TTICSGHDAD
160 170 180 190 200
TEDDPSLADL PQALDLSQQP HSSGLSCLSQ WKSVLSPGSA AQPSSCSISA
210 220 230 240 250
SSTGSSLQGH QERAEPRGGS LAKVSSSLEP VVPQEPSSVV GLGPRPQWSP
260 270 280 290 300
QPVFSGGDAS GLGRRRLSFQ AEYWACVLPD SLPPSPDRHS PLWNPNKEYE
310 320 330 340 350
DLLDYTYPLR PGPQLPKHLD SRVPADPVLQ DSGVDLDSFS VSPASTLKSP
360 370 380 390 400
TNVSPNCPPA EATALPFSGP REPSLKQWPS RVPQKQGGMG LASWSQLAST
410 420 430 440 450
PRAPGSRDAR WERREPALRG AKDRLTIGKH LDMGSPQLRT RDRGWPSPRP
460 470 480 490 500
EREKRTSQSA RRPTCTESRW KSEEEVESDD EYLALPARLT QVSSLVSYLG
510 520 530 540 550
SISTLVTLPT GDIKGQSPLE VSDSDGPASF PSSSSQSQLP PGAALQGSGD
560 570 580 590 600
PEGQNPCFLR SFVRAHDSAG EGSLGSSQAL GVSSGLLKTR PSLPARLDRW
610 620 630 640 650
PFSDPDVEGQ LPRKGGEQGK ESLVQCVKTF CCQLEELICW LYNVADVTDH
660 670 680 690 700
GTAARSNLTS LKSSLQLYRQ FKKDIDEHQS LTESVLQKGE ILLQCLLENT
710 720 730 740 750
PVLEDVLGRI AKQSGELESH ADRLYDSILA SLDMLAGCTL IPDKKPMAAM

EHPCEGV
Length:757
Mass (Da):81,102
Last modified:April 26, 2005 - v2
Checksum:i3FC969065CD5D2D7
GO
Isoform 2 (identifier: Q76N32-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     492-628: Missing.

Note: No experimental confirmation available.
Show »
Length:620
Mass (Da):67,040
Checksum:iB4E2D7D474AD4134
GO

Sequence cautioni

The sequence BAA25508 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05079427R → G. Corresponds to variant dbSNP:rs12611491Ensembl.1
Natural variantiVAR_02236374G → S1 PublicationCorresponds to variant dbSNP:rs7572857Ensembl.1
Natural variantiVAR_050795397L → P. Corresponds to variant dbSNP:rs35501092Ensembl.1
Natural variantiVAR_050796462R → C. Corresponds to variant dbSNP:rs35694840Ensembl.1
Natural variantiVAR_050797473E → Q. Corresponds to variant dbSNP:rs35089924Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013476492 – 628Missing in isoform 2. 1 PublicationAdd BLAST137

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011154 mRNA. Translation: BAA25508.2. Different initiation.
AC007386 Genomic DNA. Translation: AAF03518.2.
AK299373 mRNA. Translation: BAG61363.1.
CH471053 Genomic DNA. Translation: EAW99926.1.
CH471053 Genomic DNA. Translation: EAW99927.1.
CH471053 Genomic DNA. Translation: EAW99928.1.
CH471053 Genomic DNA. Translation: EAW99929.1.
BC002982 mRNA. Translation: AAH02982.1.
BC004873 mRNA. Translation: AAH04873.1.
CCDSiCCDS1880.2. [Q76N32-1]
CCDS82457.1. [Q76N32-2]
RefSeqiNP_001306029.1. NM_001319100.1. [Q76N32-1]
NP_001306030.1. NM_001319101.1. [Q76N32-2]
NP_055962.2. NM_015147.2. [Q76N32-1]
UniGeneiHs.709257.

Genome annotation databases

EnsembliENST00000260569; ENSP00000260569; ENSG00000011523. [Q76N32-2]
ENST00000377990; ENSP00000367229; ENSG00000011523. [Q76N32-1]
GeneIDi23177.
KEGGihsa:23177.
UCSCiuc002sdk.5. human. [Q76N32-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCEP68_HUMAN
AccessioniPrimary (citable) accession number: Q76N32
Secondary accession number(s): B4DRQ1
, D6W5F1, D6W5F2, O60326, Q9BQ18, Q9UDM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: October 25, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot