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Protein

Transcription factor MafG

Gene

Mafg

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF-E2. Activates globin gene expression when associated with NF-E2 (By similarity). May be involved in signal transduction of extracellular H+.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor MafG
Alternative name(s):
V-maf musculoaponeurotic fibrosarcoma oncogene homolog G
Gene namesi
Name:Mafg
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi619953. Mafg.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation2 Publications

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 189189Transcription factor MafGPRO_0000368188Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki41 – 41Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki41 – 41Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei80 – 801N6-acetyllysineCurated
Modified residuei87 – 871N6-acetyllysineCurated
Modified residuei98 – 981N6-acetyllysineCurated
Modified residuei103 – 1031N6-acetyllysineCurated
Modified residuei151 – 1511PhosphoserineBy similarity

Post-translational modificationi

Acetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NFE2, but has no effect on binding NFE2 (By similarity).By similarity
Sumoylation at Lys-41 is required for active transcriptional repression.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

PTM databases

iPTMnetiQ76MX4.
PhosphoSiteiQ76MX4.

Expressioni

Tissue specificityi

High expression in the ventral medulla surface (VMS), heart and skeletal muscle. Lower expression in the cerebral cortex, cerebellum, liver, stomach and intestine.1 Publication

Inductioni

Increased levels in the VMS after hypercapnic stimulation.2 Publications

Gene expression databases

BgeeiENSRNOG00000036697.
GenevisibleiQ76MX4. RN.

Interactioni

Subunit structurei

Homodimer or heterodimer (By similarity). Homodimerization leads to transcriptional repression. Forms high affinity heterodimers with members of the CNC-bZIP family such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3. Interacts with NFE2; the interaction results in transactivation activation. Interacts with CREBBP; the interaction leads to acetylation of the basic region of MAFG and stimulation of NFE2 transcriptional activity through increased DNA binding (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ76MX4.
SMRiQ76MX4. Positions 51-91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini78 – 14164bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 10324Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni106 – 12015Leucine-zipperPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the bZIP family. Maf subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074549.
HOGENOMiHOG000045475.
InParanoidiQ76MX4.
KOiK09037.
OMAiITASMGP.
OrthoDBiEOG091G0H46.
PhylomeDBiQ76MX4.
TreeFamiTF325689.

Family and domain databases

Gene3Di1.10.880.10. 1 hit.
InterProiIPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR028551. MafG.
IPR008917. TF_DNA-bd.
IPR024874. Transciption_factor_Maf_fam.
[Graphical view]
PANTHERiPTHR10129. PTHR10129. 2 hits.
PTHR10129:SF15. PTHR10129:SF15. 2 hits.
PfamiPF03131. bZIP_Maf. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
SUPFAMiSSF47454. SSF47454. 1 hit.
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q76MX4-1) [UniParc]FASTAAdd to basket
Also known as: MafG2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTPNKGNKA LKVSGEAGLG VCWAGHSYSA LTPCAVPLQV KREPGENGTS
60 70 80 90 100
LTDEELVTMS VRELNQHLRG LSKEEIIQLK QRRRTLKNRG YAASCRVKRV
110 120 130 140 150
TQKEELEKQK AELQQEVEKL ASENASMKLE LDALRSKYEA LQNFARTVAR
160 170 180
SPVAPARGPL AAGLGPLVPG KVAATSVITI VKSKTDARS
Length:189
Mass (Da):20,533
Last modified:April 14, 2009 - v2
Checksum:i65F5CF89284DC9D4
GO
Isoform 2 (identifier: Q76MX4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     14-40: Missing.

Note: May be due to intron retention.
Show »
Length:162
Mass (Da):17,877
Checksum:iC7FF19614EB95C7D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei14 – 4027Missing in isoform 2. 2 PublicationsVSP_036798Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026487 mRNA. Translation: BAA85331.1.
AB050011 mRNA. Translation: BAB41097.1.
CH473948 Genomic DNA. Translation: EDM06874.1.
CH473948 Genomic DNA. Translation: EDM06875.1.
CH473948 Genomic DNA. Translation: EDM06876.1.
BC078828 mRNA. Translation: AAH78828.1.
RefSeqiNP_071781.1. NM_022386.2. [Q76MX4-2]
XP_006247976.1. XM_006247914.2. [Q76MX4-1]
XP_006247978.1. XM_006247916.2. [Q76MX4-1]
XP_006247979.1. XM_006247917.2. [Q76MX4-1]
XP_006247980.1. XM_006247918.2. [Q76MX4-1]
XP_006247981.1. XM_006247919.2. [Q76MX4-1]
XP_006247983.1. XM_006247921.2. [Q76MX4-2]
XP_006247984.1. XM_006247922.2. [Q76MX4-2]
XP_006247985.1. XM_006247923.2. [Q76MX4-2]
XP_008766704.1. XM_008768482.1. [Q76MX4-2]
UniGeneiRn.17361.

Genome annotation databases

EnsembliENSRNOT00000054970; ENSRNOP00000051853; ENSRNOG00000036697. [Q76MX4-2]
GeneIDi64188.
KEGGirno:64188.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026487 mRNA. Translation: BAA85331.1.
AB050011 mRNA. Translation: BAB41097.1.
CH473948 Genomic DNA. Translation: EDM06874.1.
CH473948 Genomic DNA. Translation: EDM06875.1.
CH473948 Genomic DNA. Translation: EDM06876.1.
BC078828 mRNA. Translation: AAH78828.1.
RefSeqiNP_071781.1. NM_022386.2. [Q76MX4-2]
XP_006247976.1. XM_006247914.2. [Q76MX4-1]
XP_006247978.1. XM_006247916.2. [Q76MX4-1]
XP_006247979.1. XM_006247917.2. [Q76MX4-1]
XP_006247980.1. XM_006247918.2. [Q76MX4-1]
XP_006247981.1. XM_006247919.2. [Q76MX4-1]
XP_006247983.1. XM_006247921.2. [Q76MX4-2]
XP_006247984.1. XM_006247922.2. [Q76MX4-2]
XP_006247985.1. XM_006247923.2. [Q76MX4-2]
XP_008766704.1. XM_008768482.1. [Q76MX4-2]
UniGeneiRn.17361.

3D structure databases

ProteinModelPortaliQ76MX4.
SMRiQ76MX4. Positions 51-91.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ76MX4.
PhosphoSiteiQ76MX4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000054970; ENSRNOP00000051853; ENSRNOG00000036697. [Q76MX4-2]
GeneIDi64188.
KEGGirno:64188.

Organism-specific databases

CTDi4097.
RGDi619953. Mafg.

Phylogenomic databases

GeneTreeiENSGT00550000074549.
HOGENOMiHOG000045475.
InParanoidiQ76MX4.
KOiK09037.
OMAiITASMGP.
OrthoDBiEOG091G0H46.
PhylomeDBiQ76MX4.
TreeFamiTF325689.

Enzyme and pathway databases

ReactomeiR-RNO-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

PROiQ76MX4.

Gene expression databases

BgeeiENSRNOG00000036697.
GenevisibleiQ76MX4. RN.

Family and domain databases

Gene3Di1.10.880.10. 1 hit.
InterProiIPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR028551. MafG.
IPR008917. TF_DNA-bd.
IPR024874. Transciption_factor_Maf_fam.
[Graphical view]
PANTHERiPTHR10129. PTHR10129. 2 hits.
PTHR10129:SF15. PTHR10129:SF15. 2 hits.
PfamiPF03131. bZIP_Maf. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
SUPFAMiSSF47454. SSF47454. 1 hit.
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAFG_RAT
AccessioniPrimary (citable) accession number: Q76MX4
Secondary accession number(s): Q99N83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: September 7, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.