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Protein

Baicalein 7-O-glucuronosyltransferase

Gene

UBGAT-I

Organism
Scutellaria baicalensis (Baical skullcap)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the production of glucuronosylated baicalein, a flavonoid that shows antiallergic, anti-HIV and antitumor activities. Can use baicalein, scutellarein and wogonin as substrates, but not chrysin, apigenin, luteolin, quercetin, formononetin and daidzein. Highly specific for UDP-glucuronate (UDP-GlcUA) and no activity with UDP-glucose or UDP-galacturonic acid.1 Publication

Catalytic activityi

UDP-D-glucuronate + baicalein = UDP + baicalin.1 Publication

Enzyme regulationi

Inhibited by copper, zinc and iron, p-Chloromercuri-benzoic acid (PCMBA) and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), but not by N-ethylmaleimide (NEM), dithioerythritol (DTE), calcium or magnesium.1 Publication

Kineticsi

  1. KM=52.4 µM for baicalein1 Publication
  2. KM=109 µM for scutellarein1 Publication
  3. KM=106.5 µM for wogonin1 Publication
  4. KM=72.9 µM for UDP-glucuronic acid1 Publication
  1. Vmax=257 nmol/sec/mg enzyme with baicalein as substrate1 Publication
  2. Vmax=290 nmol/sec/mg enzyme with scutellarein as substrate1 Publication
  3. Vmax=182 nmol/sec/mg enzyme with wogonin as substrate1 Publication

pH dependencei

Optimum pH is 7.5.1 Publication

Temperature dependencei

Optimum temperature is 30-40 degrees Celsius.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.253. 5639.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Baicalein 7-O-glucuronosyltransferase (EC:2.4.1.253)
Alternative name(s):
UDP-glucuronate:baicalein 7-O-glucuronosyltransferase
Gene namesi
Name:UBGAT-I
OrganismiScutellaria baicalensis (Baical skullcap)
Taxonomic identifieri65409 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeScutellarioideaeScutellaria

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004121081 – 441Baicalein 7-O-glucuronosyltransferaseAdd BLAST441

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ76MR7.
SMRiQ76MR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi17 – 20Poly-Ile4

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q76MR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVLAKFISK NHPSVPIIII SNAPESAAAS VAAIPSISYH RLPLPEIPPD
60 70 80 90 100
MTTDRVELFF ELPRLSNPNL LTALQQISQK TRIRAVILDF FCNAAFEVPT
110 120 130 140 150
SLNIPTYYYF SAGTPTAILT LYFETIDETI PVDLQDLNDY VDIPGLPPIH
160 170 180 190 200
CLDIPVALSP RKSLVYKSSV DISKNLRRSA GILVNGFDAL EFRAIGSHSQ
210 220 230 240 250
RPMHFKGPTP PVYFIGPLVG DVDTKAGSEE HECLRWLDTQ PSKSVVFLCF
260 270 280 290 300
GRRGVFSAKQ LKETAAALEN SGHRFLWSVR NPPELKKATG SDEPDLDELL
310 320 330 340 350
PEGFLERTKD RGFVIKSWAP QKEVLAHDSV GGFVTHCGRS SVSEGVWFGV
360 370 380 390 400
PMIGWPVDAE LRLNRAVMVD DLQVALPLEE EAGGFVTAAE LEKRVRELME
410 420 430 440
TKAGKAVRQR VTELKLSARA AVAENGSSLN DLKKFLHATR D
Length:441
Mass (Da):48,654
Last modified:July 5, 2004 - v1
Checksum:iC25DB7A0AC292B0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042277 mRNA. Translation: BAC98300.1.

Genome annotation databases

KEGGiag:BAC98300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042277 mRNA. Translation: BAC98300.1.

3D structure databases

ProteinModelPortaliQ76MR7.
SMRiQ76MR7.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:BAC98300.

Enzyme and pathway databases

BRENDAi2.4.1.253. 5639.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBGAT_SCUBA
AccessioniPrimary (citable) accession number: Q76MR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.