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Protein

Heparan-sulfate 6-O-sulfotransferase 2

Gene

HS6ST2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate (By similarity). May also play a role in limb development.By similarity1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135SubstrateBy similarity1
Binding sitei140SubstrateBy similarity1
Active sitei145Proton acceptorBy similarity1
Binding sitei145SubstrateBy similarity1
Binding sitei180PAPSBy similarity1
Binding sitei188PAPSBy similarity1
Binding sitei192SubstrateBy similarity1
Binding sitei199SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

ReactomeiR-GGA-2022928 HS-GAG biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan-sulfate 6-O-sulfotransferase 2 (EC:2.8.2.-)
Short name:
HS6ST-2
Short name:
cHS6ST-2
Gene namesi
Name:HS6ST2
ORF Names:RCJMB04_19a20
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 403LumenalSequence analysisAdd BLAST375

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001908071 – 403Heparan-sulfate 6-O-sulfotransferase 2Add BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi259N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi315N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi389N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ76LW2
PRIDEiQ76LW2

Expressioni

Developmental stagei

Expressed in the developing wing bud. At stage 31, widely expressed with a higher level in limb and head.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000009781

Structurei

3D structure databases

SMRiQ76LW2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni88 – 96PAPS bindingBy similarity9
Regioni118 – 119Substrate bindingBy similarity2
Regioni312 – 314PAPS bindingBy similarity3
Regioni318 – 319PAPS bindingBy similarity2

Sequence similaritiesi

Belongs to the sulfotransferase 6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3955 Eukaryota
ENOG410XSW4 LUCA
GeneTreeiENSGT00390000013468
HOGENOMiHOG000007772
HOVERGENiHBG083012
InParanoidiQ76LW2
KOiK08102
PhylomeDBiQ76LW2

Family and domain databases

InterProiView protein in InterPro
IPR010635 Heparan_SO4-6-sulfoTrfase
IPR027417 P-loop_NTPase
IPR005331 Sulfotransferase
PANTHERiPTHR12812 PTHR12812, 2 hits
PfamiView protein in Pfam
PF03567 Sulfotransfer_2, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequencei

Sequence statusi: Complete.

Q76LW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDRSHKVLL ALVMLFLFAV IVLQYVCPGT ECQLLRLRAL SPAAAADPYR
60 70 80 90 100
AEDETPARFV PRFNFSAGDL LRRVDFNIKG DDLIVFLHIQ KTGGTTFGRH
110 120 130 140 150
LVRNIQLEQP CECRAGQKKC TCHRPGKRET WLFSRFSTGW SCGLHADWTE
160 170 180 190 200
LTNCVPSVVD SKKEVRLRPS RNFYYITILR DPVSRYLSEW RHVQRGATWK
210 220 230 240 250
ASLHVCDGRS PTTEELPSCY TGDDWSGCSL QEFMDCPYNL ANNRQVRMLS
260 270 280 290 300
DLSLVGCYNL SVMPEEQRNK VLLDSAKENL KRMAFFGLTE FQRKTQYLFE
310 320 330 340 350
KTFNMNFISP FTQYNSTRAS SVEIDEQTQQ RIEALNFLDM ELYDYAKDLF
360 370 380 390 400
LQRYQYMRQK EHQEARRKRQ EQRKILRAKQ AHLREQGENS SSTDYLGNVE

RWR
Length:403
Mass (Da):47,175
Last modified:July 5, 2004 - v1
Checksum:i03BEE030927BF3BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38R → H in CAG32152 (PubMed:15642098).Curated1
Sequence conflicti215 – 223Missing in CAG32152 (PubMed:15642098).Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071190 mRNA Translation: BAD02830.1
AJ720493 mRNA Translation: CAG32152.1
RefSeqiNP_989821.1, NM_204490.1
UniGeneiGga.2632

Genome annotation databases

EnsembliENSGALT00000067058; ENSGALP00000048682; ENSGALG00000034250
GeneIDi395150
KEGGigga:395150

Similar proteinsi

Entry informationi

Entry nameiH6ST2_CHICK
AccessioniPrimary (citable) accession number: Q76LW2
Secondary accession number(s): Q5ZJE1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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