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Protein

Heparan-sulfate 6-O-sulfotransferase 2

Gene

HS6ST2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate (By similarity). May also play a role in limb development.By similarity1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135SubstrateBy similarity1
Binding sitei140SubstrateBy similarity1
Active sitei145Proton acceptorBy similarity1
Binding sitei145SubstrateBy similarity1
Binding sitei180PAPSBy similarity1
Binding sitei188PAPSBy similarity1
Binding sitei192SubstrateBy similarity1
Binding sitei199SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

ReactomeiR-GGA-2022928. HS-GAG biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan-sulfate 6-O-sulfotransferase 2 (EC:2.8.2.-)
Short name:
HS6ST-2
Short name:
cHS6ST-2
Gene namesi
Name:HS6ST2
ORF Names:RCJMB04_19a20
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 4

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 403LumenalSequence analysisAdd BLAST375

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001908071 – 403Heparan-sulfate 6-O-sulfotransferase 2Add BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi259N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi315N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi389N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ76LW2.

Expressioni

Developmental stagei

Expressed in the developing wing bud. At stage 31, widely expressed with a higher level in limb and head.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000009781.

Structurei

3D structure databases

SMRiQ76LW2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni88 – 96PAPS bindingBy similarity9
Regioni118 – 119Substrate bindingBy similarity2
Regioni312 – 314PAPS bindingBy similarity3
Regioni318 – 319PAPS bindingBy similarity2

Sequence similaritiesi

Belongs to the sulfotransferase 6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3955. Eukaryota.
ENOG410XSW4. LUCA.
GeneTreeiENSGT00390000013468.
HOGENOMiHOG000007772.
HOVERGENiHBG083012.
InParanoidiQ76LW2.
KOiK08102.
PhylomeDBiQ76LW2.

Family and domain databases

InterProiView protein in InterPro
IPR010635. Heparan_SO4-6-sulfoTrfase.
IPR027417. P-loop_NTPase.
IPR005331. Sulfotransferase.
PANTHERiPTHR12812. PTHR12812. 2 hits.
PfamiView protein in Pfam
PF03567. Sulfotransfer_2. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q76LW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDRSHKVLL ALVMLFLFAV IVLQYVCPGT ECQLLRLRAL SPAAAADPYR
60 70 80 90 100
AEDETPARFV PRFNFSAGDL LRRVDFNIKG DDLIVFLHIQ KTGGTTFGRH
110 120 130 140 150
LVRNIQLEQP CECRAGQKKC TCHRPGKRET WLFSRFSTGW SCGLHADWTE
160 170 180 190 200
LTNCVPSVVD SKKEVRLRPS RNFYYITILR DPVSRYLSEW RHVQRGATWK
210 220 230 240 250
ASLHVCDGRS PTTEELPSCY TGDDWSGCSL QEFMDCPYNL ANNRQVRMLS
260 270 280 290 300
DLSLVGCYNL SVMPEEQRNK VLLDSAKENL KRMAFFGLTE FQRKTQYLFE
310 320 330 340 350
KTFNMNFISP FTQYNSTRAS SVEIDEQTQQ RIEALNFLDM ELYDYAKDLF
360 370 380 390 400
LQRYQYMRQK EHQEARRKRQ EQRKILRAKQ AHLREQGENS SSTDYLGNVE

RWR
Length:403
Mass (Da):47,175
Last modified:July 5, 2004 - v1
Checksum:i03BEE030927BF3BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38R → H in CAG32152 (PubMed:15642098).Curated1
Sequence conflicti215 – 223Missing in CAG32152 (PubMed:15642098).Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071190 mRNA. Translation: BAD02830.1.
AJ720493 mRNA. Translation: CAG32152.1.
RefSeqiNP_989821.1. NM_204490.1.
UniGeneiGga.2632.

Genome annotation databases

EnsembliENSGALT00000067058; ENSGALP00000048682; ENSGALG00000034250.
GeneIDi395150.
KEGGigga:395150.

Similar proteinsi

Entry informationi

Entry nameiH6ST2_CHICK
AccessioniPrimary (citable) accession number: Q76LW2
Secondary accession number(s): Q5ZJE1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: July 5, 2004
Last modified: October 25, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families