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Protein

Heparan-sulfate 6-O-sulfotransferase 2

Gene

HS6ST2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate (By similarity). May also play a role in limb development.By similarity1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.

GO - Molecular functioni

GO - Biological processi

  • limb development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan-sulfate 6-O-sulfotransferase 2 (EC:2.8.2.-)
Short name:
HS6ST-2
Short name:
cHS6ST-2
Gene namesi
Name:HS6ST2
ORF Names:RCJMB04_19a20
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini29 – 403375LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Heparan-sulfate 6-O-sulfotransferase 2PRO_0000190807Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi64 – 641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ76LW2.

Expressioni

Developmental stagei

Expressed in the developing wing bud. At stage 31, widely expressed with a higher level in limb and head.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000009781.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 9775'-phosphosulfate-bindingSequence Analysis
Regioni180 – 18893'-phosphate bindingSequence Analysis

Sequence similaritiesi

Belongs to the sulfotransferase 6 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG302961.
HOGENOMiHOG000007772.
HOVERGENiHBG083012.
InParanoidiQ76LW2.
KOiK08102.
PhylomeDBiQ76LW2.

Family and domain databases

InterProiIPR010635. Heparan_SO4-6-sulfoTrfase.
IPR027417. P-loop_NTPase.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12812. PTHR12812. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q76LW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDRSHKVLL ALVMLFLFAV IVLQYVCPGT ECQLLRLRAL SPAAAADPYR
60 70 80 90 100
AEDETPARFV PRFNFSAGDL LRRVDFNIKG DDLIVFLHIQ KTGGTTFGRH
110 120 130 140 150
LVRNIQLEQP CECRAGQKKC TCHRPGKRET WLFSRFSTGW SCGLHADWTE
160 170 180 190 200
LTNCVPSVVD SKKEVRLRPS RNFYYITILR DPVSRYLSEW RHVQRGATWK
210 220 230 240 250
ASLHVCDGRS PTTEELPSCY TGDDWSGCSL QEFMDCPYNL ANNRQVRMLS
260 270 280 290 300
DLSLVGCYNL SVMPEEQRNK VLLDSAKENL KRMAFFGLTE FQRKTQYLFE
310 320 330 340 350
KTFNMNFISP FTQYNSTRAS SVEIDEQTQQ RIEALNFLDM ELYDYAKDLF
360 370 380 390 400
LQRYQYMRQK EHQEARRKRQ EQRKILRAKQ AHLREQGENS SSTDYLGNVE

RWR
Length:403
Mass (Da):47,175
Last modified:July 5, 2004 - v1
Checksum:i03BEE030927BF3BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381R → H in CAG32152 (PubMed:15642098).Curated
Sequence conflicti215 – 2239Missing in CAG32152 (PubMed:15642098).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071190 mRNA. Translation: BAD02830.1.
AJ720493 mRNA. Translation: CAG32152.1.
RefSeqiNP_989821.1. NM_204490.1.
UniGeneiGga.2632.

Genome annotation databases

GeneIDi395150.
KEGGigga:395150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071190 mRNA. Translation: BAD02830.1.
AJ720493 mRNA. Translation: CAG32152.1.
RefSeqiNP_989821.1. NM_204490.1.
UniGeneiGga.2632.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000009781.

Proteomic databases

PaxDbiQ76LW2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi395150.
KEGGigga:395150.

Organism-specific databases

CTDi90161.

Phylogenomic databases

eggNOGiNOG302961.
HOGENOMiHOG000007772.
HOVERGENiHBG083012.
InParanoidiQ76LW2.
KOiK08102.
PhylomeDBiQ76LW2.

Miscellaneous databases

NextBioi20815242.
PROiQ76LW2.

Family and domain databases

InterProiIPR010635. Heparan_SO4-6-sulfoTrfase.
IPR027417. P-loop_NTPase.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12812. PTHR12812. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Distinctive expression patterns of heparan sulfate O-sulfotransferases and regional differences in heparan sulfate structure in chick limb buds."
    Nogami K., Suzuki H., Habuchi H., Ishiguro N., Iwata H., Kimata K.
    J. Biol. Chem. 279:8219-8229(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.
  3. "Functional analysis of chick heparan sulfate 6-O-sulfotransferases in limb bud development."
    Kobayashi T., Habuchi H., Nogami K., Ashikari-Hada S., Tamura K., Ide H., Kimata K.
    Dev. Growth Differ. 52:146-156(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN LIMB BUD DEVELOPMENT.

Entry informationi

Entry nameiH6ST2_CHICK
AccessioniPrimary (citable) accession number: Q76LW2
Secondary accession number(s): Q5ZJE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.