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Q76LT8

- UBP48_RAT

UniProt

Q76LT8 - UBP48_RAT

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Protein
Ubiquitin carboxyl-terminal hydrolase 48
Gene
Usp48
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Nucleophile By similarity
Active sitei353 – 3531Proton acceptor By similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.068.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 48 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 48
Synaptic ubiquitin-specific protease
Short name:
synUSP
Ubiquitin thioesterase 48
Ubiquitin-specific-processing protease 48
Gene namesi
Name:Usp48
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi735213. Usp48.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: In neuronal cells, it localizes to dendrites, as well as somas.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. membrane raft Source: RGD
  3. nucleus Source: UniProtKB-SubCell
  4. postsynaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Ubiquitin carboxyl-terminal hydrolase 48
PRO_0000249525Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei887 – 8871Phosphoserine By similarity
Modified residuei888 – 8881Phosphoserine By similarity
Modified residuei889 – 8891Phosphoserine By similarity
Modified residuei957 – 9571N6-acetyllysine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ76LT8.

PTM databases

PhosphoSiteiQ76LT8.

Expressioni

Tissue specificityi

Present in the brain, in particular in the postsynaptic density and the dendritic lipid raft fractions (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ76LT8.

Interactioni

Subunit structurei

Interacts with TRAF2 and RELA By similarity.

Protein-protein interaction databases

BioGridi263601. 1 interaction.
STRINGi10116.ENSRNOP00000052796.

Structurei

3D structure databases

ProteinModelPortaliQ76LT8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini89 – 421333USP
Add
BLAST
Domaini460 – 55495DUSP 1
Add
BLAST
Domaini569 – 692124DUSP 2
Add
BLAST
Domaini712 – 825114DUSP 3
Add
BLAST
Domaini930 – 101081Ubiquitin-like
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 3 DUSP domains.
Contains 1 USP domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000231639.
HOVERGENiHBG080368.
KOiK11858.
PhylomeDBiQ76LT8.

Family and domain databases

InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51283. DUSP. 3 hits.
PS50053. UBIQUITIN_2. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q76LT8-1 [UniParc]FASTAAdd to Basket

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MAPRLQLEKA AWRWAETVRP EEVSQEHIET AYRIWLEPCI RGVCRRNCRG     50
NPNCLVGIGE HIWLGEIDEN SFHNIDDPNC ERRKKNSFVG LTNLGASCYV 100
NTFLQVWFLN LELRQALYLC PSTCSDYTKG DGIRGGKDYE PQTICEHLQY 150
LFALLQNSNR RYIDPSGFVK ALGLDTGQQQ DAQESSKLFM SLLEDTLSKQ 200
KNPDVRNVVQ QQFCGEYAYV TVCSQCGRES KLVSKFYELE LNIQGHKQLT 250
DCISEFLKEE RLEGDNRYFC ENCQSKQNAT RKIRLLSLPC TLNLQLMRFV 300
FDRQTGHKKK LNAYIGFSES LDMEPYVEHK GGSFVYELSA VLIHRGVSAY 350
SGHYIAHVKD PQSGDWYKFN DEDIEKMEGK KLQLGIEEDL TEPSKSQTRK 400
PKCGKGTHCS RNAYMLVYRL QTQEKNHTMV QVPAFLQELV DRDNSKFEEW 450
CVEMAEMRRQ SVDKGRAKHE EVKELYQRLP AGAEPYEFVS LEWLQKWLDE 500
STPTKPIDNN ACLCSHDKLH PDKISIMKRI SEYAADIFYS RYGGGPRLTV 550
KALCKDCVVE RCRILRLKNQ LNEDYKTVNN LLKATMKGSD GFWVGKSSLR 600
SWRQLALEQL DEQDGEAEQS NGKINGSPFS KDESKEEKKE EEEELNFNED 650
ILCPHGELSI SENERRLVSQ EAWSKLQQYF PKAPEFPSYK ECCSQCKILE 700
REGEENEALH KMIAKEQKTS LPNLFQDKNR PCLSNWPEDT DALYIVSHFF 750
LDEWRKFVRK PARSTPVSSV GNAALLCPHG GLMFTFPSLT KEDSKLIALI 800
WPSEWQMIQK LFVVDKVIKI TRIEVGDVNP SQTQYISEPN LCPDCREGLL 850
CQQQKDLREY TQATIYVHKV VDNKKVMKDS APELNVSSSE TEEDKEEAKP 900
DGEKDPDFNQ SNGGTKRQKT SQQGYVAYQK QVIRRSTRHR KVRGEKALLV 950
SANQTLKELK IQIMHAFSVA PFDQNLSIDG KILNDDCATL GTLGVIPESV 1000
ILLKADEPIA DYAAMDDVMQ VCMPEEGFKG TGLLGH 1036
Length:1,036
Mass (Da):118,787
Last modified:July 5, 2004 - v1
Checksum:iEEC407C21D4BC539
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB073880 mRNA. Translation: BAD00009.1.
RefSeqiNP_942080.1. NM_198785.1.
UniGeneiRn.100022.

Genome annotation databases

GeneIDi362636.
KEGGirno:362636.
UCSCiRGD:735213. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB073880 mRNA. Translation: BAD00009.1 .
RefSeqi NP_942080.1. NM_198785.1.
UniGenei Rn.100022.

3D structure databases

ProteinModelPortali Q76LT8.
ModBasei Search...

Protein-protein interaction databases

BioGridi 263601. 1 interaction.
STRINGi 10116.ENSRNOP00000052796.

Protein family/group databases

MEROPSi C19.068.

PTM databases

PhosphoSitei Q76LT8.

Proteomic databases

PRIDEi Q76LT8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 362636.
KEGGi rno:362636.
UCSCi RGD:735213. rat.

Organism-specific databases

CTDi 84196.
RGDi 735213. Usp48.

Phylogenomic databases

eggNOGi COG5077.
HOGENOMi HOG000231639.
HOVERGENi HBG080368.
KOi K11858.
PhylomeDBi Q76LT8.

Miscellaneous databases

NextBioi 680703.
PROi Q76LT8.

Gene expression databases

Genevestigatori Q76LT8.

Family and domain databases

InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEi PS51283. DUSP. 3 hits.
PS50053. UBIQUITIN_2. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A novel ubiquitin-specific protease, synUSP, is localized at the post-synaptic density and post-synaptic lipid raft."
    Tian Q.B., Okano A., Nakayama K., Miyazawa S., Endo S., Suzuki T.
    J. Neurochem. 87:665-675(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiUBP48_RAT
AccessioniPrimary (citable) accession number: Q76LT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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