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Q76LT8

- UBP48_RAT

UniProt

Q76LT8 - UBP48_RAT

Protein

Ubiquitin carboxyl-terminal hydrolase 48

Gene

Usp48

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites.1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei98 – 981NucleophilePROSITE-ProRule annotation
    Active sitei353 – 3531Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.068.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 48 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 48
    Synaptic ubiquitin-specific protease
    Short name:
    synUSP
    Ubiquitin thioesterase 48
    Ubiquitin-specific-processing protease 48
    Gene namesi
    Name:Usp48
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi735213. Usp48.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: In neuronal cells, it localizes to dendrites, as well as somas.1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane raft Source: RGD
    3. nucleus Source: UniProtKB-SubCell
    4. postsynaptic membrane Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10361036Ubiquitin carboxyl-terminal hydrolase 48PRO_0000249525Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei887 – 8871PhosphoserineBy similarity
    Modified residuei888 – 8881PhosphoserineBy similarity
    Modified residuei889 – 8891PhosphoserineBy similarity
    Modified residuei957 – 9571N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiQ76LT8.

    PTM databases

    PhosphoSiteiQ76LT8.

    Expressioni

    Tissue specificityi

    Present in the brain, in particular in the postsynaptic density and the dendritic lipid raft fractions (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriQ76LT8.

    Interactioni

    Subunit structurei

    Interacts with TRAF2 and RELA.By similarity

    Protein-protein interaction databases

    BioGridi263601. 1 interaction.
    STRINGi10116.ENSRNOP00000052796.

    Structurei

    3D structure databases

    ProteinModelPortaliQ76LT8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini89 – 421333USPAdd
    BLAST
    Domaini460 – 55495DUSP 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini569 – 692124DUSP 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini712 – 825114DUSP 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini930 – 101081Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 3 DUSP domains.PROSITE-ProRule annotation
    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5077.
    HOGENOMiHOG000231639.
    HOVERGENiHBG080368.
    KOiK11858.
    PhylomeDBiQ76LT8.

    Family and domain databases

    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS51283. DUSP. 3 hits.
    PS50053. UBIQUITIN_2. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q76LT8-1 [UniParc]FASTAAdd to Basket

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    MAPRLQLEKA AWRWAETVRP EEVSQEHIET AYRIWLEPCI RGVCRRNCRG     50
    NPNCLVGIGE HIWLGEIDEN SFHNIDDPNC ERRKKNSFVG LTNLGASCYV 100
    NTFLQVWFLN LELRQALYLC PSTCSDYTKG DGIRGGKDYE PQTICEHLQY 150
    LFALLQNSNR RYIDPSGFVK ALGLDTGQQQ DAQESSKLFM SLLEDTLSKQ 200
    KNPDVRNVVQ QQFCGEYAYV TVCSQCGRES KLVSKFYELE LNIQGHKQLT 250
    DCISEFLKEE RLEGDNRYFC ENCQSKQNAT RKIRLLSLPC TLNLQLMRFV 300
    FDRQTGHKKK LNAYIGFSES LDMEPYVEHK GGSFVYELSA VLIHRGVSAY 350
    SGHYIAHVKD PQSGDWYKFN DEDIEKMEGK KLQLGIEEDL TEPSKSQTRK 400
    PKCGKGTHCS RNAYMLVYRL QTQEKNHTMV QVPAFLQELV DRDNSKFEEW 450
    CVEMAEMRRQ SVDKGRAKHE EVKELYQRLP AGAEPYEFVS LEWLQKWLDE 500
    STPTKPIDNN ACLCSHDKLH PDKISIMKRI SEYAADIFYS RYGGGPRLTV 550
    KALCKDCVVE RCRILRLKNQ LNEDYKTVNN LLKATMKGSD GFWVGKSSLR 600
    SWRQLALEQL DEQDGEAEQS NGKINGSPFS KDESKEEKKE EEEELNFNED 650
    ILCPHGELSI SENERRLVSQ EAWSKLQQYF PKAPEFPSYK ECCSQCKILE 700
    REGEENEALH KMIAKEQKTS LPNLFQDKNR PCLSNWPEDT DALYIVSHFF 750
    LDEWRKFVRK PARSTPVSSV GNAALLCPHG GLMFTFPSLT KEDSKLIALI 800
    WPSEWQMIQK LFVVDKVIKI TRIEVGDVNP SQTQYISEPN LCPDCREGLL 850
    CQQQKDLREY TQATIYVHKV VDNKKVMKDS APELNVSSSE TEEDKEEAKP 900
    DGEKDPDFNQ SNGGTKRQKT SQQGYVAYQK QVIRRSTRHR KVRGEKALLV 950
    SANQTLKELK IQIMHAFSVA PFDQNLSIDG KILNDDCATL GTLGVIPESV 1000
    ILLKADEPIA DYAAMDDVMQ VCMPEEGFKG TGLLGH 1036
    Length:1,036
    Mass (Da):118,787
    Last modified:July 5, 2004 - v1
    Checksum:iEEC407C21D4BC539
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073880 mRNA. Translation: BAD00009.1.
    RefSeqiNP_942080.1. NM_198785.1.
    UniGeneiRn.100022.

    Genome annotation databases

    GeneIDi362636.
    KEGGirno:362636.
    UCSCiRGD:735213. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB073880 mRNA. Translation: BAD00009.1 .
    RefSeqi NP_942080.1. NM_198785.1.
    UniGenei Rn.100022.

    3D structure databases

    ProteinModelPortali Q76LT8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 263601. 1 interaction.
    STRINGi 10116.ENSRNOP00000052796.

    Protein family/group databases

    MEROPSi C19.068.

    PTM databases

    PhosphoSitei Q76LT8.

    Proteomic databases

    PRIDEi Q76LT8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 362636.
    KEGGi rno:362636.
    UCSCi RGD:735213. rat.

    Organism-specific databases

    CTDi 84196.
    RGDi 735213. Usp48.

    Phylogenomic databases

    eggNOGi COG5077.
    HOGENOMi HOG000231639.
    HOVERGENi HBG080368.
    KOi K11858.
    PhylomeDBi Q76LT8.

    Miscellaneous databases

    NextBioi 680703.
    PROi Q76LT8.

    Gene expression databases

    Genevestigatori Q76LT8.

    Family and domain databases

    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS51283. DUSP. 3 hits.
    PS50053. UBIQUITIN_2. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel ubiquitin-specific protease, synUSP, is localized at the post-synaptic density and post-synaptic lipid raft."
      Tian Q.B., Okano A., Nakayama K., Miyazawa S., Endo S., Suzuki T.
      J. Neurochem. 87:665-675(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiUBP48_RAT
    AccessioniPrimary (citable) accession number: Q76LT8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3