Q76LT8 (UBP48_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 48
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 48
Synaptic ubiquitin-specific protease
Short name=synUSP
Ubiquitin thioesterase 48
Ubiquitin-specific-processing protease 48

Gene names

Name:

Usp48

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1036 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites. Ref.1
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.1
Subunit structure	Interacts with TRAF2 and RELA By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: In neuronal cells, it localizes to dendrites, as well as somas. Ref.1
Tissue specificity	Present in the brain, in particular in the postsynaptic density and the dendritic lipid raft fractions (at protein level). Ref.1
Sequence similarities	Belongs to the peptidase C19 family. Contains 3 DUSP domains. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Molecular function	Hydrolase Protease Thiol protease
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane raft Inferred from direct assay Ref.1. Source: RGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from direct assay Ref.1. Source: RGD
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1036	1036	Ubiquitin carboxyl-terminal hydrolase 48		PRO_0000249525
Regions
	Domain	460 – 554	95	DUSP 1
	Domain	569 – 692	124	DUSP 2
	Domain	712 – 825	114	DUSP 3
	Domain	930 – 1010	81	Ubiquitin-like
Sites
	Active site	98	1	Nucleophile By similarity
	Active site	353	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	887	1	Phosphoserine By similarity
	Modified residue	888	1	Phosphoserine By similarity
	Modified residue	889	1	Phosphoserine By similarity
	Modified residue	957	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q76LT8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EEC407C21D4BC539	FASTA	1,036	118,787
	10 20 30 40 50 60 MAPRLQLEKA AWRWAETVRP EEVSQEHIET AYRIWLEPCI RGVCRRNCRG NPNCLVGIGE 70 80 90 100 110 120 HIWLGEIDEN SFHNIDDPNC ERRKKNSFVG LTNLGASCYV NTFLQVWFLN LELRQALYLC 130 140 150 160 170 180 PSTCSDYTKG DGIRGGKDYE PQTICEHLQY LFALLQNSNR RYIDPSGFVK ALGLDTGQQQ 190 200 210 220 230 240 DAQESSKLFM SLLEDTLSKQ KNPDVRNVVQ QQFCGEYAYV TVCSQCGRES KLVSKFYELE 250 260 270 280 290 300 LNIQGHKQLT DCISEFLKEE RLEGDNRYFC ENCQSKQNAT RKIRLLSLPC TLNLQLMRFV 310 320 330 340 350 360 FDRQTGHKKK LNAYIGFSES LDMEPYVEHK GGSFVYELSA VLIHRGVSAY SGHYIAHVKD 370 380 390 400 410 420 PQSGDWYKFN DEDIEKMEGK KLQLGIEEDL TEPSKSQTRK PKCGKGTHCS RNAYMLVYRL 430 440 450 460 470 480 QTQEKNHTMV QVPAFLQELV DRDNSKFEEW CVEMAEMRRQ SVDKGRAKHE EVKELYQRLP 490 500 510 520 530 540 AGAEPYEFVS LEWLQKWLDE STPTKPIDNN ACLCSHDKLH PDKISIMKRI SEYAADIFYS 550 560 570 580 590 600 RYGGGPRLTV KALCKDCVVE RCRILRLKNQ LNEDYKTVNN LLKATMKGSD GFWVGKSSLR 610 620 630 640 650 660 SWRQLALEQL DEQDGEAEQS NGKINGSPFS KDESKEEKKE EEEELNFNED ILCPHGELSI 670 680 690 700 710 720 SENERRLVSQ EAWSKLQQYF PKAPEFPSYK ECCSQCKILE REGEENEALH KMIAKEQKTS 730 740 750 760 770 780 LPNLFQDKNR PCLSNWPEDT DALYIVSHFF LDEWRKFVRK PARSTPVSSV GNAALLCPHG 790 800 810 820 830 840 GLMFTFPSLT KEDSKLIALI WPSEWQMIQK LFVVDKVIKI TRIEVGDVNP SQTQYISEPN 850 860 870 880 890 900 LCPDCREGLL CQQQKDLREY TQATIYVHKV VDNKKVMKDS APELNVSSSE TEEDKEEAKP 910 920 930 940 950 960 DGEKDPDFNQ SNGGTKRQKT SQQGYVAYQK QVIRRSTRHR KVRGEKALLV SANQTLKELK 970 980 990 1000 1010 1020 IQIMHAFSVA PFDQNLSIDG KILNDDCATL GTLGVIPESV ILLKADEPIA DYAAMDDVMQ 1030 VCMPEEGFKG TGLLGH

References

[1]

"A novel ubiquitin-specific protease, synUSP, is localized at the post-synaptic density and post-synaptic lipid raft." Tian Q.B., Okano A., Nakayama K., Miyazawa S., Endo S., Suzuki T. J. Neurochem. 87:665-675(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB073880 mRNA. Translation: BAD00009.1.
IPI	IPI00400620.
RefSeq	NP_942080.1. NM_198785.1.
UniGene	Rn.100022.
3D structure databases
HSSP	HSSP built from PDB template 2AYN based on UniProtKB P54578.
ProteinModelPortal	Q76LT8.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000052796.
Protein family/group databases
MEROPS	C19.068.
PTM databases
PhosphoSite	Q76LT8.
Proteomic databases
PRIDE	Q76LT8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	362636.
KEGG	rno:362636.
UCSC	RGD:735213. rat.
Organism-specific databases
CTD	84196.
RGD	735213. Usp48.
Phylogenomic databases
eggNOG	COG5077.
HOGENOM	HOG000231639.
HOVERGEN	HBG080368.
KO	K11858.
OrthoDB	EOG4GF3DC.
Gene expression databases
ArrayExpress	Q76LT8.
Genevestigator	Q76LT8.
GermOnline	ENSRNOG00000013602. Rattus norvegicus.
Family and domain databases
InterPro	IPR006615. Pept_C19_DUSP. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR019955. Ubiquitin_supergroup. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS51283. DUSP. 3 hits. PS00299. UBIQUITIN_1. False negative. PS50053. UBIQUITIN_2. 1 hit. PS00972. UCH_2_1. False negative. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	680703.

Entry information

Entry name

UBP48_RAT

Accession

Primary (citable) accession number: Q76LT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program