Q76LD0 (CRDL1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chordin-like protein 1

Alternative name(s):
Kohjirin
Neuralin-1
Neurogenesin-1
Ventroptin

Gene names

Name:	Chrdl1
Synonyms:	Kjr, Ng1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. Plays a role during anterior segment eye development By similarity. Ref.1
Subcellular location	Secreted Potential.
Developmental stage	Ubiquitously expressed in neurogenic regions of the embryonic brain. Ref.1
Sequence similarities	Contains 3 VWFC domains.
Caution	The sequence shown has an N-terminus according to mammalian orthologs. The original sequence from Ref.1 gives a longer N-terminus and suggests a signal sequence of 97 AA.
Sequence caution	The sequence BAD06447.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Osteogenesis
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell fate determination Inferred from mutant phenotype Ref.1. Source: RGD compound eye development Inferred from sequence or structural similarity. Source: UniProtKB neuron differentiation Inferred from mutant phenotype Ref.1. Source: RGD ossification Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Traceable author statement Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 447	425	Chordin-like protein 1		PRO_0000223676
Regions
	Domain	30 – 95	66	VWFC 1
	Domain	108 – 174	67	VWFC 2
	Domain	253 – 318	66	VWFC 3
	Motif	174 – 176	3	Cell attachment site Potential
Amino acid modifications
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Glycosylation	286	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q76LD0 [UniParc]. Last modified February 21, 2006. Version 2. Checksum: 5B76AC14008C0838	FASTA	447	50,560
	10 20 30 40 50 60 MEGIKYIASL VFFFVFLEAS KTEPVKHSET YCMFQDKKYR VGEKWHPYLE PYGLVYCVNC 70 80 90 100 110 120 ICSENGNVLC SRVRCPTLHC LSPVHIPHLC CPRCPDSLPP MNSKVTSKSC EYNGTTYQHG 130 140 150 160 170 180 ELFIAEGLFQ NRQPNQCSQC SCSEGNVYCG LKTCPKLTCA FPVSVPDSCC RVCRGDGELS 190 200 210 220 230 240 WEHSDADIFR QPANREARHS YLRSPYDPPP SRQAGGLPRF AGSRSHRGAV IDSQQASGTI 250 260 270 280 290 300 VQIVINNKHK HGQVCVSNGK TYSHGESWHP NLRAFGIVEC VLCTCNVTKQ ECKKIHCPNR 310 320 330 340 350 360 YPCKYPQKLD GKCCKVCPEE PPSQNFDSKG SFCGEETMPV YEAVLVEDGE TARKVALETE 370 380 390 400 410 420 KPPQVEVHVW TIRKGILQHF HIEKISKEMF GGLHHFKLVT RTTMNQWKIF AEGEAQLSQM 430 440 CSSRVCRTEL EDLVQVLYLE RPEKDHC

References

[1]

"A novel secretory factor, neurogenesin-1, provides neurogenic environmental cues for neural stem cells in the adult hippocampus." Ueki T., Tanaka M., Yamashita K., Mikawa S., Qiu Z., Maragakis N.J., Hevner R.F., Miura N., Sugimura H., Sato K. J. Neurosci. 23:11732-11740(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB080636 mRNA. Translation: BAD06447.1. Different initiation.
IPI	IPI00400469.
RefSeq	NP_955796.1. NM_199502.1.
UniGene	Rn.211154.
3D structure databases
ProteinModelPortal	Q76LD0.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000006008.
PTM databases
PhosphoSite	Q76LD0.
Proteomic databases
PaxDb	Q76LD0.
PRIDE	Q76LD0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	363455.
KEGG	rno:363455.
Organism-specific databases
CTD	91851.
RGD	735215. Chrdl1.
Phylogenomic databases
eggNOG	NOG147797.
HOGENOM	HOG000049211.
HOVERGEN	HBG051113.
InParanoid	Q76LD0.
OrthoDB	EOG46Q6SD.
Gene expression databases
ArrayExpress	Q76LD0.
Genevestigator	Q76LD0.
GermOnline	ENSRNOG00000004330. Rattus norvegicus.
Family and domain databases
InterPro	IPR001007. VWF_C. [Graphical view]
Pfam	PF00093. VWC. 3 hits. [Graphical view]
SMART	SM00214. VWC. 3 hits. [Graphical view]
PROSITE	PS01208. VWFC_1. 3 hits. PS50184. VWFC_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	683371.

Entry information

Entry name

CRDL1_RAT

Accession

Primary (citable) accession number: Q76LD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	February 21, 2006
Last modified:	April 3, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program