Q76L34 (PCYAA_ASTLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Photoactivated adenylate cyclase subunit alpha
EC=4.6.1.1

Alternative name(s):
Photoactivated adenylyl cyclase subunit alpha

Gene names

Name:

pacA

Organism

Astasia longa (Euglenophycean alga)

Taxonomic identifier

3037 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Euglenida › Euglenales › Euglena

Protein attributes

Sequence length	1019 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a photoreceptor for the step-up photophobic response. Ref.1
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate. UniProtKB Q8S9F2
Cofactor	FAD By similarity. UniProtKB Q8S9F2
Subunit structure	Heterotetramer of two alpha and two beta subunits By similarity. UniProtKB Q8S9F2
Subcellular location	Cell projection › cilium › flagellum Ref.2.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 BLUF domains. Contains 2 guanylate cyclase domains.

Ontologies

Keywords
Biological process	Sensory transduction cAMP biosynthesis
Cellular component	Cell projection Cilium Flagellum
Domain	Repeat
Ligand	ATP-binding Chromophore FAD Flavoprotein Nucleotide-binding
Molecular function	Lyase Photoreceptor protein Receptor
Gene Ontology (GO)
Biological process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cilium Inferred from electronic annotation. Source: UniProtKB-KW flagellum Inferred from direct assay Ref.2. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from direct assay Ref.1. Source: UniProtKB photoreceptor activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1019	1019	Photoactivated adenylate cyclase subunit alpha		PRO_0000233946
Regions
	Domain	55 – 148	94	BLUF 1
	Domain	204 – 332	129	Guanylate cyclase 1
	Domain	467 – 559	93	BLUF 2
	Domain	615 – 744	130	Guanylate cyclase 2

Sequences

	Sequence		Length	Mass (Da)
	Q76L34 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 69F402B6002414A7	FASTA	1,019	112,078
	10 20 30 40 50 60 MYILVWKEGQ QIKTFQDLEE CGQFQIASNI TDGQIFSVSV TPTMSKGGET GETQLRRLMY 70 80 90 100 110 120 LSASTEPEKC NAEYLADMAH VATLRNKQIG VSGFLLYSSP FFFQVIEGTD EDLDFLFAKI 130 140 150 160 170 180 SSDPRHERCI VLANGPCTGR MYGEWHMKDS HIDNITKHPA IKTILFQIAR SFSSMWSYLP 190 200 210 220 230 240 KNAANMLLLG KNPNKQAPEP MSVVVTFIYL VEFSSILAHP GLTEQCADIL AAFVDACVRN 250 260 270 280 290 300 VEGTGGQVAK FITGICMAYW PINRAEDALV GLQQLSEDLA ELRSQQPPGS ALSLIYSRCG 310 320 330 340 350 360 VHYGRALLCN AGFRKADFTL LGDCINTASR ITSLSVKLKV PLLLSFEVRC LLGDEMREEL 370 380 390 400 410 420 ESAGLHKVKG RDKPVQVYQF NAPELDSAVV RTKIEQFNPG RYRALCPVKP YDSLHPAQRP 430 440 450 460 470 480 PIFDDTPRDS QPKVNQMQRR DSLVDRLSMI AKLAFPSSMM VGGESQLITL TYISQAAHPM 490 500 510 520 530 540 SRLDLSSIQR VSFSRNESSN ITGSLLYVNG LFVQTLEGPK SAVVSLYLKI RQDKRHKDVV 550 560 570 580 590 600 AVFMAPIEER VYGSPLDMTA ATEEMLATFP PLQDVLSQLA KSFISLETYV PSTVVRYLTA 610 620 630 640 650 660 GNNPRNLQPV SVEVVMLATD ICSFTPLSER CSLTEVWTIC NTFIDACTSA ICNEGGEVIK 670 680 690 700 710 720 LIGDCVTAYF PPTAADNAVH ACQEIVSFCA QLRDAFRDVL DCRSVVACGV GLDFGQVIMA 730 740 750 760 770 780 QCGSLGMTEF VVAGEVSARV MEVEALTREA GRAIVITEPV ADRLSPKLRD TGIVPCQEGV 790 800 810 820 830 840 DGVPCYGILG VEWELDVAII KKNIYGFHEA RAQAALKKVD DGTNAPGRGA PAAGVPSSPK 850 860 870 880 890 900 ARALGRTSSV SSYTPDLNET LDPRMAESVF NDLCNQRGDA PNNSIAAKLR QAANDDRLDL 910 920 930 940 950 960 GRMLQGPHEL MPVLQAIKQL TNLRMLNMSD NFVDDNNVGE LAESCIPMRS LQVLDLSNNP 970 980 990 1000 1010 GLTKVIALKR LIKHNTQIRE ILLNGTRIAP MEQRKLQSSM NVNRMCASTD SKSSHKYDH

References

[1]	"Photoactivated adenylyl cyclase controls phototaxis in the flagellate Euglena gracilis." Ntefidou M., Iseki M., Watanabe M., Lebert M., Haeder D.-P. Plant Physiol. 133:1517-1521(2003) [PubMed: 14630964] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena gracilis mutant strains." Ntefidou M., Haeder D.-P. Photochem. Photobiol. Sci. 4:732-739(2005) [PubMed: 16121285] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB085169 mRNA. Translation: BAD04848.1.
3D structure databases
ProteinModelPortal	Q76L34.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR001792. Acylphosphatase-like. IPR007024. BLUF_domain. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 2 hits.
Pfam	PF04940. BLUF. 2 hits. PF00211. Guanylate_cyc. 2 hits. [Graphical view]
SMART	SM01034. BLUF. 2 hits. SM00044. CYCc. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 2 hits. SSF54975. Acylphosphatase. 2 hits.
PROSITE	PS50925. BLUF. 2 hits. PS00452. GUANYLATE_CYCLASE_1. False negative. PS50125. GUANYLATE_CYCLASE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCYAA_ASTLO

Accession

Primary (citable) accession number: Q76L34

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)