Q76L33 (PCYAB_ASTLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Photoactivated adenylate cyclase subunit beta
EC=4.6.1.1

Alternative name(s):
Photoactivated adenylyl cyclase subunit beta

Gene names

Name:

pacB

Organism

Astasia longa (Euglenophycean alga)

Taxonomic identifier

3037 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Euglenida › Euglenales › Euglena

Protein attributes

Sequence length	860 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a photoreceptor for the step-up photophobic response. Ref.1
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate. UniProtKB Q8S9F1
Cofactor	FAD By similarity. UniProtKB Q8S9F1
Subunit structure	Heterotetramer of two alpha and two beta subunits By similarity. UniProtKB Q8S9F1
Subcellular location	Cell projection › cilium › flagellum Ref.2.
Sequence similarities	Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 2 BLUF domains. Contains 2 guanylate cyclase domains.

Ontologies

Keywords
Biological process	Sensory transduction cAMP biosynthesis
Cellular component	Cell projection Cilium Flagellum
Domain	Repeat
Ligand	ATP-binding Chromophore FAD Flavoprotein Nucleotide-binding
Molecular function	Lyase Photoreceptor protein Receptor
Gene Ontology (GO)
Biological process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cilium Inferred from electronic annotation. Source: UniProtKB-KW flagellum Inferred from direct assay Ref.2. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from direct assay Ref.1. Source: UniProtKB photoreceptor activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 860	860	Photoactivated adenylate cyclase subunit beta		PRO_0000233947
Regions
	Domain	56 – 149	94	BLUF 1
	Domain	205 – 333	129	Guanylate cyclase 1
	Domain	471 – 563	93	BLUF 2
	Domain	619 – 748	130	Guanylate cyclase 2

Sequences

	Sequence		Length	Mass (Da)
	Q76L33 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2FFD4DDDD239B39D	FASTA	860	94,375
	10 20 30 40 50 60 MYILVWKRGQ QIKTFHTLDE AANFKAASNI DEAQMFSITV APAISASGGS NEATNLRRLM 70 80 90 100 110 120 YLSKSTNPEE CNPQFLAEMA RVATLRNREI GVSGFLMYSS PFFFQVIEGT DEDLDFLFAK 130 140 150 160 170 180 ISADPRHERC IVLANGPCTG RMYGDWHMKD SHIDSITTHP AMKTILYQIA RSFSSMWSYL 190 200 210 220 230 240 PKSAGNMLLL GKDPAAQPPE PMSVVVTFIY LVEFGSILSN PNLTEQAAEV LSTFVDVCVK 250 260 270 280 290 300 NVEGSGGNIA KFITGICMAY WPINRTEDAL NAIQQISEDL AQLRSQQAPG SAISLMYSQA 310 320 330 340 350 360 GVHYGRAMLC NAGSRKSDFT LLGDCINTTS RIATLAKKLK TPLLFSFEVR CLLGDEMREE 370 380 390 400 410 420 VEGAGMHQVK GRDKPVVVYQ FPGPELDVEM VRQKIEHFTP GRFRCQMPVV EYESLPISQR 430 440 450 460 470 480 PPIFDDTPKG KPRPRTPGYG GRQRSDSLVD RLIMIAKLAG PSISATGDST LTTLTYISQA 490 500 510 520 530 540 TRPMSRLDLS AIMRTATRRN AQLSITGTLL YVNGLFVQTL EGPKDAMVNL YLKIRQDPRH 550 560 570 580 590 600 TDVVTVHMAP IQERAYPAEW TLTSATEEML ATFPPLQDVL SQLAKSFTSL ETYVPSTVVR 610 620 630 640 650 660 YLTAGNNPRN LMPVSCGVVM LATDICSFTS LTEKSSLTEV WMICNTFIDA CTSAICQEGG 670 680 690 700 710 720 EVIKLIGDCV TAYFPGNGAD SAVAAAQELF TFCRQLREAF VDVLDVRGCV SCGVGLDYGQ 730 740 750 760 770 780 VVMAQCGSLG LTEYVVAGAV SARVMEVESI TREVGYAIVI TEPVADRLSP QLRDHGIIPT 790 800 810 820 830 840 PQAIEGLPCY GIAGEEFELD VDSIKRGIKA LHAARSGEKP LALEPEEAKQ DYRVSPGRMR 850 860 HGDSGRRSNS AQGKRSTQVR

References

[1]	"Photoactivated adenylyl cyclase controls phototaxis in the flagellate Euglena gracilis." Ntefidou M., Iseki M., Watanabe M., Lebert M., Haeder D.-P. Plant Physiol. 133:1517-1521(2003) [PubMed: 14630964] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena gracilis mutant strains." Ntefidou M., Haeder D.-P. Photochem. Photobiol. Sci. 4:732-739(2005) [PubMed: 16121285] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB085170 mRNA. Translation: BAD04849.1.
3D structure databases
ProteinModelPortal	Q76L33.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR001792. Acylphosphatase-like. IPR007024. BLUF_domain. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 2 hits.
Pfam	PF04940. BLUF. 2 hits. PF00211. Guanylate_cyc. 2 hits. [Graphical view]
SMART	SM01034. BLUF. 2 hits. SM00044. CYCc. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 2 hits. SSF54975. Acylphosphatase. 2 hits.
PROSITE	PS50925. BLUF. 2 hits. PS00452. GUANYLATE_CYCLASE_1. False negative. PS50125. GUANYLATE_CYCLASE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCYAB_ASTLO

Accession

Primary (citable) accession number: Q76L33

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)