Reviewed, UniProtKB/Swiss-Prot Q76KP1 (B4GN4_HUMAN)

Names and origin

Protein names

Recommended name:
    N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1
      Short name=NGalNAc-T1
    EC=2.4.1.244
Alternative name(s):
    Beta-1,4-N-acetylgalactosaminyltransferase IV
      Short name=Beta4GalNAc-T4
      Short name=Beta4GalNAcT4

Gene names

Name:

B4GALNT4

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1039 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transfers N-acetylgalactosamine (GalNAc) from UDP-GalNAc to N-acetylglucosamine-beta-benzyl with a beta-1,4-linkage to form N,N'-diacetyllactosediamine, GalNAc-beta-1,4-GlcNAc structures in N-linked glycans and probably O-linked glycans.
Catalytic activity	UDP-N-acetyl-D-galactosamine + N-acetyl-beta-D-glucosaminyl group = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl group. Ref.1
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Highly expressed in ovary, adult and fetal brain. Also expressed in fetal kidney and lung. Ref.1
Sequence similarities	Belongs to the chondroitin N-acetylgalactosaminyltransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1039	1039	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1		PRO_0000252370
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 36	21	Signal-anchor for type II membrane protein Potential
	Topological domain	37 – 1039	1003	Lumenal Potential
	Compositional bias	449 – 590	142	Pro-rich
Amino acid modifications
	Glycosylation	105	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	942	1	V → M in BAB71566. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q76KP1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1D8D9E07AFC8DF70	FASTA	1,039	116,513
	10 20 30 40 50 60 MPRLPVKKIR KQMKLLLLLL LLSCAAWLTY VHLGLVRQGR ALRQRLGYGR DGEKLTSETD 70 80 90 100 110 120 GRGVHAAPST QRAEDSSESR EEEQAPEGRD LDMLFPGGAG RLPLNFTHQT PPWREEYKGQ 130 140 150 160 170 180 VNLHVFEDWC GGAVGHLRRN LHFPLFPHTR TTVKKLAVSP KWKNYGLRIF GFIHPARDGD 190 200 210 220 230 240 VQFSVASDDN SEFWLSLDES PAAAQLVAFV GKTGSEWTAP GEFTKFSSQV SKPRRLMASR 250 260 270 280 290 300 RYYFELLHKQ DDRGSDHVEV GWRAFLPGLK FEVISSAHIS LYTDESALKM DHVAHVPQSP 310 320 330 340 350 360 ASHVGGRPPQ EETSADMLRP DPRDTFFLTP RMESSSLENV LEPCAYAPTY VVKDFPIARY 370 380 390 400 410 420 QGLQFVYLSF VYPNDYTRLT HMETDNKCFY RESPLYLERF GFYKYMKMDK EEGDEDEEDE 430 440 450 460 470 480 VQRRAFLFLN PDDFLDDEDE GELLDSLEPT EAAPPRSGPQ SPAPAAPAQP GATLAPPTPP 490 500 510 520 530 540 RPRDGGTPRH SRALSWAARA ARPLPLFLGR APPPRPAVEQ PPPKVYVTRV RPGQRASPRA 550 560 570 580 590 600 PAPRAPWPPF PGVFLHPRPL PRVQLRAPPR PPRPHGRRTG GPQATQPRPP ARAQATQGGR 610 620 630 640 650 660 EGQARTLGPA APTVDSNLSS EARPVTSFLS LSQVSGPQLP GEGEEEEEGE DDGAPGDEAA 670 680 690 700 710 720 SEDSEEAAGP ALGRWREDAI DWQRTFSVGA VDFELLRSDW NDLRCNVSGN LQLPEAEAVD 730 740 750 760 770 780 VTAQYMERLN ARHGGRFALL RIVNVEKRRD SARGSRFLLE LELQERGGGR LRLSEYVFLR 790 800 810 820 830 840 LPGARVGDAD GESPEPAPAA SVRPDGRPEL CRPLRLAWRQ DVMVHFIVPV KNQARWVAQF 850 860 870 880 890 900 LADMAALHAR TGDSRFSVVL VDFESEDMDV ERALRAARLP RYQYLRRTGN FERSAGLQAG 910 920 930 940 950 960 VDAVEDASSI VFLCDLHIHF PPNILDGIRK HCVEGRLAFA PVVMRLSCGS SPRDPHGYWE 970 980 990 1000 1010 1020 VNGFGLFGIY KSDFDRVGGM NTEEFRDQWG GEDWELLDRV LQAGLEVERL RLRNFYHHYH 1030 SKRGMWSVRS RKGSRTGAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N'-diacetyllactosediamine." Gotoh M., Sato T., Kiyohara K., Kameyama A., Kikuchi N., Kwon Y.-D., Ishizuka Y., Iwai T., Nakanishi H., Narimatsu H. FEBS Lett. 562:134-140(2004) [PubMed: 15044014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 707-1039. Tissue: Cerebellum.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB089939 mRNA. Translation: BAD06471.1. AK057774 mRNA. Translation: BAB71566.1. Different initiation.
IPI	IPI00065312.
RefSeq	NP_848632.2.
UniGene	Hs.148074
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
PTM databases
PhosphoSite	Q76KP1.
Proteomic databases
PRIDE	Q76KP1.
Genome annotation databases
Ensembl	ENST00000329962; ENSP00000328277; ENSG00000182272; Homo sapiens. [Genome view]
GeneID	338707.
KEGG	hsa:338707.
UCSC	uc001lpb.1. human.
Organism-specific databases
CTD	338707.
GeneCards	GC11P000366.
H-InvDB	HIX0019949.
HGNC	HGNC:26315. B4GALNT4.
PharmGKB	PA142672565.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q76KP1.
OMA	HVEVGWR.
Enzyme and pathway databases
BRENDA	2.4.1.244. 247. 2.4.1.92. 247.
Gene expression databases
ArrayExpress	Q76KP1.
Bgee	Q76KP1.
CleanEx	HS_B4GALNT4.
Genevestigator	Q76KP1.
GermOnline	ENSG00000182272. Homo sapiens.
Family and domain databases
InterPro	IPR008428. Chond_GalNAc. IPR011658. PA14. [Graphical view]
PANTHER	PTHR12369. CHGN. 1 hit.
Pfam	PF05679. CHGN. 1 hit. PF07691. PA14. 1 hit. [Graphical view]
SMART	SM00758. PA14. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	97118.

Entry information

Entry name

B4GN4_HUMAN

Accession

Primary (citable) accession number: Q76KP1
Secondary accession number(s): Q96LV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.