Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1

Gene

B4GALNT4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transfers N-acetylgalactosamine (GalNAc) from UDP-GalNAc to N-acetylglucosamine-beta-benzyl with a beta-1,4-linkage to form N,N'-diacetyllactosediamine, GalNAc-beta-1,4-GlcNAc structures in N-linked glycans and probably O-linked glycans.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-beta-D-glucosaminyl group = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl group.1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BRENDAi2.4.1.244. 2681.
2.4.1.92. 2681.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1 (EC:2.4.1.244)
Short name:
NGalNAc-T1
Alternative name(s):
Beta-1,4-N-acetylgalactosaminyltransferase IV
Short name:
Beta4GalNAc-T4
Short name:
Beta4GalNAcT4
Gene namesi
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:26315. B4GALNT4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence analysisAdd
BLAST
Transmembranei16 – 3621Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini37 – 10391003LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142672565.

Polymorphism and mutation databases

BioMutaiB4GALNT4.
DMDMi74712926.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10391039N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1PRO_0000252370Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ76KP1.
PaxDbiQ76KP1.
PeptideAtlasiQ76KP1.
PRIDEiQ76KP1.

PTM databases

iPTMnetiQ76KP1.
PhosphoSiteiQ76KP1.

Expressioni

Tissue specificityi

Highly expressed in ovary, adult and fetal brain. Also expressed in fetal kidney and lung.1 Publication

Gene expression databases

BgeeiQ76KP1.
CleanExiHS_B4GALNT4.
ExpressionAtlasiQ76KP1. baseline and differential.
GenevisibleiQ76KP1. HS.

Organism-specific databases

HPAiHPA053126.

Interactioni

Protein-protein interaction databases

BioGridi130784. 5 interactions.
STRINGi9606.ENSP00000328277.

Structurei

3D structure databases

ProteinModelPortaliQ76KP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi449 – 590142Pro-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3588. Eukaryota.
ENOG410XNYM. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000071102.
HOVERGENiHBG080886.
InParanoidiQ76KP1.
KOiK09657.
OMAiPPWREEY.
OrthoDBiEOG7CG6ZB.
PhylomeDBiQ76KP1.
TreeFamiTF318303.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
IPR011658. PA14_dom.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q76KP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRLPVKKIR KQMKLLLLLL LLSCAAWLTY VHLGLVRQGR ALRQRLGYGR
60 70 80 90 100
DGEKLTSETD GRGVHAAPST QRAEDSSESR EEEQAPEGRD LDMLFPGGAG
110 120 130 140 150
RLPLNFTHQT PPWREEYKGQ VNLHVFEDWC GGAVGHLRRN LHFPLFPHTR
160 170 180 190 200
TTVKKLAVSP KWKNYGLRIF GFIHPARDGD VQFSVASDDN SEFWLSLDES
210 220 230 240 250
PAAAQLVAFV GKTGSEWTAP GEFTKFSSQV SKPRRLMASR RYYFELLHKQ
260 270 280 290 300
DDRGSDHVEV GWRAFLPGLK FEVISSAHIS LYTDESALKM DHVAHVPQSP
310 320 330 340 350
ASHVGGRPPQ EETSADMLRP DPRDTFFLTP RMESSSLENV LEPCAYAPTY
360 370 380 390 400
VVKDFPIARY QGLQFVYLSF VYPNDYTRLT HMETDNKCFY RESPLYLERF
410 420 430 440 450
GFYKYMKMDK EEGDEDEEDE VQRRAFLFLN PDDFLDDEDE GELLDSLEPT
460 470 480 490 500
EAAPPRSGPQ SPAPAAPAQP GATLAPPTPP RPRDGGTPRH SRALSWAARA
510 520 530 540 550
ARPLPLFLGR APPPRPAVEQ PPPKVYVTRV RPGQRASPRA PAPRAPWPPF
560 570 580 590 600
PGVFLHPRPL PRVQLRAPPR PPRPHGRRTG GPQATQPRPP ARAQATQGGR
610 620 630 640 650
EGQARTLGPA APTVDSNLSS EARPVTSFLS LSQVSGPQLP GEGEEEEEGE
660 670 680 690 700
DDGAPGDEAA SEDSEEAAGP ALGRWREDAI DWQRTFSVGA VDFELLRSDW
710 720 730 740 750
NDLRCNVSGN LQLPEAEAVD VTAQYMERLN ARHGGRFALL RIVNVEKRRD
760 770 780 790 800
SARGSRFLLE LELQERGGGR LRLSEYVFLR LPGARVGDAD GESPEPAPAA
810 820 830 840 850
SVRPDGRPEL CRPLRLAWRQ DVMVHFIVPV KNQARWVAQF LADMAALHAR
860 870 880 890 900
TGDSRFSVVL VDFESEDMDV ERALRAARLP RYQYLRRTGN FERSAGLQAG
910 920 930 940 950
VDAVEDASSI VFLCDLHIHF PPNILDGIRK HCVEGRLAFA PVVMRLSCGS
960 970 980 990 1000
SPRDPHGYWE VNGFGLFGIY KSDFDRVGGM NTEEFRDQWG GEDWELLDRV
1010 1020 1030
LQAGLEVERL RLRNFYHHYH SKRGMWSVRS RKGSRTGAS
Length:1,039
Mass (Da):116,513
Last modified:July 5, 2004 - v1
Checksum:i1D8D9E07AFC8DF70
GO

Sequence cautioni

The sequence BAB71566.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti942 – 9421V → M in BAB71566 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti67 – 671A → V.
Corresponds to variant rs34063493 [ dbSNP | Ensembl ].
VAR_061096
Natural varianti88 – 881G → V.
Corresponds to variant rs11822725 [ dbSNP | Ensembl ].
VAR_061097

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089939 mRNA. Translation: BAD06471.1.
AK057774 mRNA. Translation: BAB71566.1. Different initiation.
CCDSiCCDS7694.1.
RefSeqiNP_848632.2. NM_178537.4.
UniGeneiHs.148074.

Genome annotation databases

EnsembliENST00000329962; ENSP00000328277; ENSG00000182272.
GeneIDi338707.
KEGGihsa:338707.
UCSCiuc001lpb.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089939 mRNA. Translation: BAD06471.1.
AK057774 mRNA. Translation: BAB71566.1. Different initiation.
CCDSiCCDS7694.1.
RefSeqiNP_848632.2. NM_178537.4.
UniGeneiHs.148074.

3D structure databases

ProteinModelPortaliQ76KP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130784. 5 interactions.
STRINGi9606.ENSP00000328277.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

iPTMnetiQ76KP1.
PhosphoSiteiQ76KP1.

Polymorphism and mutation databases

BioMutaiB4GALNT4.
DMDMi74712926.

Proteomic databases

EPDiQ76KP1.
PaxDbiQ76KP1.
PeptideAtlasiQ76KP1.
PRIDEiQ76KP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000329962; ENSP00000328277; ENSG00000182272.
GeneIDi338707.
KEGGihsa:338707.
UCSCiuc001lpb.4. human.

Organism-specific databases

CTDi338707.
GeneCardsiB4GALNT4.
HGNCiHGNC:26315. B4GALNT4.
HPAiHPA053126.
neXtProtiNX_Q76KP1.
PharmGKBiPA142672565.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3588. Eukaryota.
ENOG410XNYM. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000071102.
HOVERGENiHBG080886.
InParanoidiQ76KP1.
KOiK09657.
OMAiPPWREEY.
OrthoDBiEOG7CG6ZB.
PhylomeDBiQ76KP1.
TreeFamiTF318303.

Enzyme and pathway databases

BRENDAi2.4.1.244. 2681.
2.4.1.92. 2681.

Miscellaneous databases

GenomeRNAii338707.
PROiQ76KP1.

Gene expression databases

BgeeiQ76KP1.
CleanExiHS_B4GALNT4.
ExpressionAtlasiQ76KP1. baseline and differential.
GenevisibleiQ76KP1. HS.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
IPR011658. PA14_dom.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N'-diacetyllactosediamine."
    Gotoh M., Sato T., Kiyohara K., Kameyama A., Kikuchi N., Kwon Y.-D., Ishizuka Y., Iwai T., Nakanishi H., Narimatsu H.
    FEBS Lett. 562:134-140(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 707-1039.
    Tissue: Cerebellum.

Entry informationi

Entry nameiB4GN4_HUMAN
AccessioniPrimary (citable) accession number: Q76KP1
Secondary accession number(s): Q96LV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.