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Protein

DNA-directed RNA polymerase I subunit RPA34

Gene

Cd3eap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Involved in UBTF-activated transcription, presumably at a step following PIC formation.1 Publication

GO - Molecular functioni

GO - Biological processi

  • rRNA transcription Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73863. RNA Polymerase I Transcription Termination.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA34
Alternative name(s):
A34.5
Anti-sense to ERCC-1 protein
Short name:
ASE-1
CD3-epsilon-associated protein
Short name:
CD3E-associated protein
RNA polymerase I-associated factor PAF49
Gene namesi
Name:Cd3eap
Synonyms:Ase1, Paf49
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1917583. Cd3eap.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • DNA-directed RNA polymerase I complex Source: MGI
  • mitochondrion Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002281211 – 399DNA-directed RNA polymerase I subunit RPA34Add BLAST399

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei80PhosphotyrosineBy similarity1
Modified residuei137PhosphoserineBy similarity1
Modified residuei173PhosphoserineBy similarity1
Modified residuei277PhosphothreonineBy similarity1
Modified residuei299PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ76KJ5.
MaxQBiQ76KJ5.
PaxDbiQ76KJ5.
PeptideAtlasiQ76KJ5.
PRIDEiQ76KJ5.

PTM databases

iPTMnetiQ76KJ5.
PhosphoSitePlusiQ76KJ5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000047649.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF (By similarity). Interacts with POLR1E/PRAF1 through its N-terminal region.By similarity1 Publication

Protein-protein interaction databases

BioGridi213987. 1 interactor.
STRINGi10090.ENSMUSP00000044653.

Structurei

3D structure databases

ProteinModelPortaliQ76KJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi360 – 368Poly-Lys9

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IVQP. Eukaryota.
ENOG4112CGI. LUCA.
GeneTreeiENSGT00450000040362.
HOGENOMiHOG000120117.
HOVERGENiHBG082101.
InParanoidiQ76KJ5.
OMAiFCAFGGN.
OrthoDBiEOG091G0H8Y.
PhylomeDBiQ76KJ5.
TreeFamiTF338162.

Family and domain databases

InterProiIPR013240. DNA-dir_RNA_pol1_su_RPA34.
[Graphical view]
PfamiPF08208. RNA_polI_A34. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q76KJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTQACSAT RFSCPPHFTE MSPDSEPSRF SLEALTGPDT ELWLIQAPAD
60 70 80 90 100
FAPQCLNGRR VPLSGSKTVK GKLDGKKHRY RVFTSSPQAR EATLLASSSE
110 120 130 140 150
AGGRLTCAPA PSGSLRIMEG PQEYLLSRVP LQLIPTSLPP QIPAGLRPRF
160 170 180 190 200
SAFGGSPPVT GPGSASALRS PTSGKRKSTR KGTDASSDTQ EAVNRHGAME
210 220 230 240 250
VKTALGNLGV SVKKRKRYFM QEEMEAKTME PVAELPVPSA TSSKKRKKSK
260 270 280 290 300
GTETSQVEHT EPVAQTEPPE GTFLFPTKKR KRQKEADGTE EVDGIVADSQ
310 320 330 340 350
PQVIVEAQEE TILLSPTKKR RKEKRQNLGM EAEMGPPGVL MVTEHSEHGL
360 370 380 390
QAEVALVSPK KTKKKKGKRV GETEAALPDD FEPQGALAPS KKKERGARL
Length:399
Mass (Da):43,082
Last modified:March 21, 2006 - v2
Checksum:iE7CE66D5FFE93EC0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90R → G in BAD02839 (PubMed:15226435).Curated1
Sequence conflicti273F → L in BAD02839 (PubMed:15226435).Curated1
Sequence conflicti313Missing in BAD02839 (PubMed:15226435).Curated1
Sequence conflicti394 – 399ERGARL → RERRTPLIPALGRQRQADF (PubMed:15226435).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB091121 mRNA. Translation: BAD02839.1.
BC029206 mRNA. Translation: AAH29206.1.
BC071199 mRNA. Translation: AAH71199.1.
CCDSiCCDS39798.1.
RefSeqiNP_665821.1. NM_145822.2.
UniGeneiMm.171370.

Genome annotation databases

EnsembliENSMUST00000047036; ENSMUSP00000044653; ENSMUSG00000047649.
GeneIDi70333.
KEGGimmu:70333.
UCSCiuc009fln.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB091121 mRNA. Translation: BAD02839.1.
BC029206 mRNA. Translation: AAH29206.1.
BC071199 mRNA. Translation: AAH71199.1.
CCDSiCCDS39798.1.
RefSeqiNP_665821.1. NM_145822.2.
UniGeneiMm.171370.

3D structure databases

ProteinModelPortaliQ76KJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213987. 1 interactor.
STRINGi10090.ENSMUSP00000044653.

PTM databases

iPTMnetiQ76KJ5.
PhosphoSitePlusiQ76KJ5.

Proteomic databases

EPDiQ76KJ5.
MaxQBiQ76KJ5.
PaxDbiQ76KJ5.
PeptideAtlasiQ76KJ5.
PRIDEiQ76KJ5.

Protocols and materials databases

DNASUi70333.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047036; ENSMUSP00000044653; ENSMUSG00000047649.
GeneIDi70333.
KEGGimmu:70333.
UCSCiuc009fln.2. mouse.

Organism-specific databases

CTDi10849.
MGIiMGI:1917583. Cd3eap.

Phylogenomic databases

eggNOGiENOG410IVQP. Eukaryota.
ENOG4112CGI. LUCA.
GeneTreeiENSGT00450000040362.
HOGENOMiHOG000120117.
HOVERGENiHBG082101.
InParanoidiQ76KJ5.
OMAiFCAFGGN.
OrthoDBiEOG091G0H8Y.
PhylomeDBiQ76KJ5.
TreeFamiTF338162.

Enzyme and pathway databases

ReactomeiR-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73863. RNA Polymerase I Transcription Termination.

Miscellaneous databases

PROiQ76KJ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047649.

Family and domain databases

InterProiIPR013240. DNA-dir_RNA_pol1_su_RPA34.
[Graphical view]
PfamiPF08208. RNA_polI_A34. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPA34_MOUSE
AccessioniPrimary (citable) accession number: Q76KJ5
Secondary accession number(s): Q8K0Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: November 2, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in human, suggesting an important biological function.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.