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Q76KJ5

- RPA34_MOUSE

UniProt

Q76KJ5 - RPA34_MOUSE

Protein

DNA-directed RNA polymerase I subunit RPA34

Gene

Cd3eap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Involved in UBTF-activated transcription, presumably at a step following PIC formation.1 Publication

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. rRNA transcription Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase I subunit RPA34
    Alternative name(s):
    A34.5
    Anti-sense to ERCC-1 protein
    Short name:
    ASE-1
    CD3-epsilon-associated protein
    Short name:
    CD3E-associated protein
    RNA polymerase I-associated factor PAF49
    Gene namesi
    Name:Cd3eap
    Synonyms:Ase1, Paf49
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1917583. Cd3eap.

    Subcellular locationi

    Nucleusnucleolus 1 Publication
    Note: Accumulates in the nucleolus of exponentially growing cells.

    GO - Cellular componenti

    1. DNA-directed RNA polymerase I complex Source: MGI
    2. mitochondrion Source: Ensembl
    3. nucleolus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 399399DNA-directed RNA polymerase I subunit RPA34PRO_0000228121Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei80 – 801PhosphotyrosineBy similarity
    Modified residuei137 – 1371PhosphoserineBy similarity
    Modified residuei173 – 1731PhosphoserineBy similarity
    Modified residuei277 – 2771PhosphothreonineBy similarity
    Modified residuei299 – 2991PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ76KJ5.
    PRIDEiQ76KJ5.

    PTM databases

    PhosphoSiteiQ76KJ5.

    Expressioni

    Gene expression databases

    BgeeiQ76KJ5.
    GenevestigatoriQ76KJ5.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF By similarity. Interacts with POLR1E/PRAF1 through its N-terminal region.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi213987. 1 interaction.
    STRINGi10090.ENSMUSP00000044653.

    Structurei

    3D structure databases

    ProteinModelPortaliQ76KJ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi360 – 3689Poly-Lys

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG45026.
    GeneTreeiENSGT00450000040362.
    HOGENOMiHOG000120117.
    HOVERGENiHBG082101.
    InParanoidiQ76KJ5.
    OMAiFCAFGGN.
    OrthoDBiEOG7VTDPJ.
    PhylomeDBiQ76KJ5.
    TreeFamiTF338162.

    Family and domain databases

    InterProiIPR013240. DNA-dir_RNA_pol1_su_RPA34.
    [Graphical view]
    PfamiPF08208. RNA_polI_A34. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q76KJ5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGTQACSAT RFSCPPHFTE MSPDSEPSRF SLEALTGPDT ELWLIQAPAD    50
    FAPQCLNGRR VPLSGSKTVK GKLDGKKHRY RVFTSSPQAR EATLLASSSE 100
    AGGRLTCAPA PSGSLRIMEG PQEYLLSRVP LQLIPTSLPP QIPAGLRPRF 150
    SAFGGSPPVT GPGSASALRS PTSGKRKSTR KGTDASSDTQ EAVNRHGAME 200
    VKTALGNLGV SVKKRKRYFM QEEMEAKTME PVAELPVPSA TSSKKRKKSK 250
    GTETSQVEHT EPVAQTEPPE GTFLFPTKKR KRQKEADGTE EVDGIVADSQ 300
    PQVIVEAQEE TILLSPTKKR RKEKRQNLGM EAEMGPPGVL MVTEHSEHGL 350
    QAEVALVSPK KTKKKKGKRV GETEAALPDD FEPQGALAPS KKKERGARL 399
    Length:399
    Mass (Da):43,082
    Last modified:March 21, 2006 - v2
    Checksum:iE7CE66D5FFE93EC0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti90 – 901R → G in BAD02839. (PubMed:15226435)Curated
    Sequence conflicti273 – 2731F → L in BAD02839. (PubMed:15226435)Curated
    Sequence conflicti313 – 3131Missing in BAD02839. (PubMed:15226435)Curated
    Sequence conflicti394 – 3996ERGARL → RERRTPLIPALGRQRQADF(PubMed:15226435)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB091121 mRNA. Translation: BAD02839.1.
    BC029206 mRNA. Translation: AAH29206.1.
    BC071199 mRNA. Translation: AAH71199.1.
    CCDSiCCDS39798.1.
    RefSeqiNP_665821.1. NM_145822.2.
    UniGeneiMm.171370.

    Genome annotation databases

    EnsembliENSMUST00000047036; ENSMUSP00000044653; ENSMUSG00000047649.
    GeneIDi70333.
    KEGGimmu:70333.
    UCSCiuc009fln.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB091121 mRNA. Translation: BAD02839.1 .
    BC029206 mRNA. Translation: AAH29206.1 .
    BC071199 mRNA. Translation: AAH71199.1 .
    CCDSi CCDS39798.1.
    RefSeqi NP_665821.1. NM_145822.2.
    UniGenei Mm.171370.

    3D structure databases

    ProteinModelPortali Q76KJ5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 213987. 1 interaction.
    STRINGi 10090.ENSMUSP00000044653.

    PTM databases

    PhosphoSitei Q76KJ5.

    Proteomic databases

    PaxDbi Q76KJ5.
    PRIDEi Q76KJ5.

    Protocols and materials databases

    DNASUi 70333.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047036 ; ENSMUSP00000044653 ; ENSMUSG00000047649 .
    GeneIDi 70333.
    KEGGi mmu:70333.
    UCSCi uc009fln.2. mouse.

    Organism-specific databases

    CTDi 10849.
    MGIi MGI:1917583. Cd3eap.

    Phylogenomic databases

    eggNOGi NOG45026.
    GeneTreei ENSGT00450000040362.
    HOGENOMi HOG000120117.
    HOVERGENi HBG082101.
    InParanoidi Q76KJ5.
    OMAi FCAFGGN.
    OrthoDBi EOG7VTDPJ.
    PhylomeDBi Q76KJ5.
    TreeFami TF338162.

    Miscellaneous databases

    NextBioi 331396.
    PROi Q76KJ5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q76KJ5.
    Genevestigatori Q76KJ5.

    Family and domain databases

    InterProi IPR013240. DNA-dir_RNA_pol1_su_RPA34.
    [Graphical view ]
    Pfami PF08208. RNA_polI_A34. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription."
      Yamamoto K., Yamamoto M., Hanada K., Nogi Y., Matsuyama T., Muramatsu M.
      Mol. Cell. Biol. 24:6338-6349(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 182-196; 218-226 AND 251-261, IDENTIFICATION IN THE RNA POL I COMPLEX, FUNCTION, INTERACTION WITH SL1 COMPLEX AND POLR1E, SUBCELLULAR LOCATION.
      Tissue: Ascitic tumor.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon and Mammary tumor.
    3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.

    Entry informationi

    Entry nameiRPA34_MOUSE
    AccessioniPrimary (citable) accession number: Q76KJ5
    Secondary accession number(s): Q8K0Y9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in human, suggesting an important biological function.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3