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Protein

DNA-directed RNA polymerase I subunit RPA34

Gene

Cd3eap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Involved in UBTF-activated transcription, presumably at a step following PIC formation.1 Publication

Miscellaneous

It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in human, suggesting an important biological function.

GO - Biological processi

  • rRNA transcription Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73863 RNA Polymerase I Transcription Termination

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA34
Alternative name(s):
A34.5
Anti-sense to ERCC-1 protein
Short name:
ASE-1
CD3-epsilon-associated protein
Short name:
CD3E-associated protein
RNA polymerase I-associated factor PAF49
Gene namesi
Name:Cd3eap
Synonyms:Ase1, Paf49
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1917583 Cd3eap

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002281211 – 399DNA-directed RNA polymerase I subunit RPA34Add BLAST399

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei80PhosphotyrosineBy similarity1
Modified residuei137PhosphoserineBy similarity1
Modified residuei173PhosphoserineBy similarity1
Modified residuei277PhosphothreonineBy similarity1
Modified residuei299PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ76KJ5
MaxQBiQ76KJ5
PaxDbiQ76KJ5
PeptideAtlasiQ76KJ5
PRIDEiQ76KJ5

PTM databases

iPTMnetiQ76KJ5
PhosphoSitePlusiQ76KJ5

Expressioni

Gene expression databases

BgeeiENSMUSG00000047649

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF (By similarity). Interacts with POLR1E/PRAF1 through its N-terminal region.By similarity1 Publication

Protein-protein interaction databases

BioGridi2139872 interactors.
STRINGi10090.ENSMUSP00000044653

Structurei

3D structure databases

ProteinModelPortaliQ76KJ5
SMRiQ76KJ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi360 – 368Poly-Lys9

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IVQP Eukaryota
ENOG4112CGI LUCA
GeneTreeiENSGT00450000040362
HOGENOMiHOG000120117
HOVERGENiHBG082101
InParanoidiQ76KJ5
OMAiPRFCAFG
OrthoDBiEOG091G0H8Y
PhylomeDBiQ76KJ5
TreeFamiTF338162

Family and domain databases

InterProiView protein in InterPro
IPR013240 DNA-dir_RNA_pol1_su_RPA34
PfamiView protein in Pfam
PF08208 RNA_polI_A34, 1 hit

Sequencei

Sequence statusi: Complete.

Q76KJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTQACSAT RFSCPPHFTE MSPDSEPSRF SLEALTGPDT ELWLIQAPAD
60 70 80 90 100
FAPQCLNGRR VPLSGSKTVK GKLDGKKHRY RVFTSSPQAR EATLLASSSE
110 120 130 140 150
AGGRLTCAPA PSGSLRIMEG PQEYLLSRVP LQLIPTSLPP QIPAGLRPRF
160 170 180 190 200
SAFGGSPPVT GPGSASALRS PTSGKRKSTR KGTDASSDTQ EAVNRHGAME
210 220 230 240 250
VKTALGNLGV SVKKRKRYFM QEEMEAKTME PVAELPVPSA TSSKKRKKSK
260 270 280 290 300
GTETSQVEHT EPVAQTEPPE GTFLFPTKKR KRQKEADGTE EVDGIVADSQ
310 320 330 340 350
PQVIVEAQEE TILLSPTKKR RKEKRQNLGM EAEMGPPGVL MVTEHSEHGL
360 370 380 390
QAEVALVSPK KTKKKKGKRV GETEAALPDD FEPQGALAPS KKKERGARL
Length:399
Mass (Da):43,082
Last modified:March 21, 2006 - v2
Checksum:iE7CE66D5FFE93EC0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90R → G in BAD02839 (PubMed:15226435).Curated1
Sequence conflicti273F → L in BAD02839 (PubMed:15226435).Curated1
Sequence conflicti313Missing in BAD02839 (PubMed:15226435).Curated1
Sequence conflicti394 – 399ERGARL → RERRTPLIPALGRQRQADF (PubMed:15226435).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB091121 mRNA Translation: BAD02839.1
BC029206 mRNA Translation: AAH29206.1
BC071199 mRNA Translation: AAH71199.1
CCDSiCCDS39798.1
RefSeqiNP_665821.1, NM_145822.2
UniGeneiMm.171370

Genome annotation databases

EnsembliENSMUST00000047036; ENSMUSP00000044653; ENSMUSG00000047649
GeneIDi70333
KEGGimmu:70333
UCSCiuc009fln.2 mouse

Similar proteinsi

Entry informationi

Entry nameiRPA34_MOUSE
AccessioniPrimary (citable) accession number: Q76KJ5
Secondary accession number(s): Q8K0Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: February 28, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome