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Q76KJ5

- RPA34_MOUSE

UniProt

Q76KJ5 - RPA34_MOUSE

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Protein

DNA-directed RNA polymerase I subunit RPA34

Gene

Cd3eap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Involved in UBTF-activated transcription, presumably at a step following PIC formation.1 Publication

GO - Molecular functioni

  1. poly(A) RNA binding Source: Ensembl

GO - Biological processi

  1. rRNA transcription Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA34
Alternative name(s):
A34.5
Anti-sense to ERCC-1 protein
Short name:
ASE-1
CD3-epsilon-associated protein
Short name:
CD3E-associated protein
RNA polymerase I-associated factor PAF49
Gene namesi
Name:Cd3eap
Synonyms:Ase1, Paf49
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1917583. Cd3eap.

Subcellular locationi

Nucleusnucleolus 1 Publication
Note: Accumulates in the nucleolus of exponentially growing cells.

GO - Cellular componenti

  1. DNA-directed RNA polymerase I complex Source: MGI
  2. mitochondrion Source: Ensembl
  3. nucleolus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399DNA-directed RNA polymerase I subunit RPA34PRO_0000228121Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei80 – 801PhosphotyrosineBy similarity
Modified residuei137 – 1371PhosphoserineBy similarity
Modified residuei173 – 1731PhosphoserineBy similarity
Modified residuei277 – 2771PhosphothreonineBy similarity
Modified residuei299 – 2991PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ76KJ5.
PaxDbiQ76KJ5.
PRIDEiQ76KJ5.

PTM databases

PhosphoSiteiQ76KJ5.

Expressioni

Gene expression databases

BgeeiQ76KJ5.
GenevestigatoriQ76KJ5.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF By similarity. Interacts with POLR1E/PRAF1 through its N-terminal region.By similarity1 Publication

Protein-protein interaction databases

BioGridi213987. 1 interaction.
STRINGi10090.ENSMUSP00000044653.

Structurei

3D structure databases

ProteinModelPortaliQ76KJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi360 – 3689Poly-Lys

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG45026.
GeneTreeiENSGT00450000040362.
HOGENOMiHOG000120117.
HOVERGENiHBG082101.
InParanoidiQ76KJ5.
OMAiFCAFGGN.
OrthoDBiEOG7VTDPJ.
PhylomeDBiQ76KJ5.
TreeFamiTF338162.

Family and domain databases

InterProiIPR013240. DNA-dir_RNA_pol1_su_RPA34.
[Graphical view]
PfamiPF08208. RNA_polI_A34. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q76KJ5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGTQACSAT RFSCPPHFTE MSPDSEPSRF SLEALTGPDT ELWLIQAPAD
60 70 80 90 100
FAPQCLNGRR VPLSGSKTVK GKLDGKKHRY RVFTSSPQAR EATLLASSSE
110 120 130 140 150
AGGRLTCAPA PSGSLRIMEG PQEYLLSRVP LQLIPTSLPP QIPAGLRPRF
160 170 180 190 200
SAFGGSPPVT GPGSASALRS PTSGKRKSTR KGTDASSDTQ EAVNRHGAME
210 220 230 240 250
VKTALGNLGV SVKKRKRYFM QEEMEAKTME PVAELPVPSA TSSKKRKKSK
260 270 280 290 300
GTETSQVEHT EPVAQTEPPE GTFLFPTKKR KRQKEADGTE EVDGIVADSQ
310 320 330 340 350
PQVIVEAQEE TILLSPTKKR RKEKRQNLGM EAEMGPPGVL MVTEHSEHGL
360 370 380 390
QAEVALVSPK KTKKKKGKRV GETEAALPDD FEPQGALAPS KKKERGARL
Length:399
Mass (Da):43,082
Last modified:March 21, 2006 - v2
Checksum:iE7CE66D5FFE93EC0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901R → G in BAD02839. (PubMed:15226435)Curated
Sequence conflicti273 – 2731F → L in BAD02839. (PubMed:15226435)Curated
Sequence conflicti313 – 3131Missing in BAD02839. (PubMed:15226435)Curated
Sequence conflicti394 – 3996ERGARL → RERRTPLIPALGRQRQADF(PubMed:15226435)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB091121 mRNA. Translation: BAD02839.1.
BC029206 mRNA. Translation: AAH29206.1.
BC071199 mRNA. Translation: AAH71199.1.
CCDSiCCDS39798.1.
RefSeqiNP_665821.1. NM_145822.2.
UniGeneiMm.171370.

Genome annotation databases

EnsembliENSMUST00000047036; ENSMUSP00000044653; ENSMUSG00000047649.
GeneIDi70333.
KEGGimmu:70333.
UCSCiuc009fln.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB091121 mRNA. Translation: BAD02839.1 .
BC029206 mRNA. Translation: AAH29206.1 .
BC071199 mRNA. Translation: AAH71199.1 .
CCDSi CCDS39798.1.
RefSeqi NP_665821.1. NM_145822.2.
UniGenei Mm.171370.

3D structure databases

ProteinModelPortali Q76KJ5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 213987. 1 interaction.
STRINGi 10090.ENSMUSP00000044653.

PTM databases

PhosphoSitei Q76KJ5.

Proteomic databases

MaxQBi Q76KJ5.
PaxDbi Q76KJ5.
PRIDEi Q76KJ5.

Protocols and materials databases

DNASUi 70333.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047036 ; ENSMUSP00000044653 ; ENSMUSG00000047649 .
GeneIDi 70333.
KEGGi mmu:70333.
UCSCi uc009fln.2. mouse.

Organism-specific databases

CTDi 10849.
MGIi MGI:1917583. Cd3eap.

Phylogenomic databases

eggNOGi NOG45026.
GeneTreei ENSGT00450000040362.
HOGENOMi HOG000120117.
HOVERGENi HBG082101.
InParanoidi Q76KJ5.
OMAi FCAFGGN.
OrthoDBi EOG7VTDPJ.
PhylomeDBi Q76KJ5.
TreeFami TF338162.

Miscellaneous databases

NextBioi 331396.
PROi Q76KJ5.
SOURCEi Search...

Gene expression databases

Bgeei Q76KJ5.
Genevestigatori Q76KJ5.

Family and domain databases

InterProi IPR013240. DNA-dir_RNA_pol1_su_RPA34.
[Graphical view ]
Pfami PF08208. RNA_polI_A34. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription."
    Yamamoto K., Yamamoto M., Hanada K., Nogi Y., Matsuyama T., Muramatsu M.
    Mol. Cell. Biol. 24:6338-6349(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 182-196; 218-226 AND 251-261, IDENTIFICATION IN THE RNA POL I COMPLEX, FUNCTION, INTERACTION WITH SL1 COMPLEX AND POLR1E, SUBCELLULAR LOCATION.
    Tissue: Ascitic tumor.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Mammary tumor.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.

Entry informationi

Entry nameiRPA34_MOUSE
AccessioniPrimary (citable) accession number: Q76KJ5
Secondary accession number(s): Q8K0Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: October 29, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in human, suggesting an important biological function.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3