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Q76KJ5 (RPA34_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase I subunit RPA34
Alternative name(s):
A34.5
Anti-sense to ERCC-1 protein
Short name=ASE-1
CD3-epsilon-associated protein
Short name=CD3E-associated protein
RNA polymerase I-associated factor PAF49
Gene names
Name:Cd3eap
Synonyms:Ase1, Paf49
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Involved in UBTF-activated transcription, presumably at a step following PIC formation. Ref.1

Subunit structure

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF By similarity. Interacts with POLR1E/PRAF1 through its N-terminal region. Ref.1

Subcellular location

Nucleus › nucleolus. Note: Accumulates in the nucleolus of exponentially growing cells. Ref.1

Miscellaneous

It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in human, suggesting an important biological function.

Sequence similarities

Belongs to the eukaryotic RPA34 RNA polymerase subunit family.

Ontologies

Keywords
   Cellular componentNucleus
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processrRNA transcription

Inferred from direct assay Ref.1. Source: MGI

   Cellular_componentDNA-directed RNA polymerase I complex

Inferred from direct assay Ref.1. Source: MGI

mitochondrion

Inferred from electronic annotation. Source: Compara

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 399399DNA-directed RNA polymerase I subunit RPA34
PRO_0000228121

Regions

Compositional bias360 – 3689Poly-Lys

Amino acid modifications

Modified residue801Phosphotyrosine By similarity
Modified residue1371Phosphoserine By similarity
Modified residue1731Phosphoserine By similarity
Modified residue2411Phosphothreonine Ref.3
Modified residue2771Phosphothreonine By similarity
Modified residue2991Phosphoserine By similarity

Experimental info

Sequence conflict901R → G in BAD02839. Ref.1
Sequence conflict2731F → L in BAD02839. Ref.1
Sequence conflict3131Missing in BAD02839. Ref.1
Sequence conflict394 – 3996ERGARL → RERRTPLIPALGRQRQADF Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q76KJ5 [UniParc].

Last modified March 21, 2006. Version 2.
Checksum: E7CE66D5FFE93EC0

FASTA39943,082
        10         20         30         40         50         60 
MAGTQACSAT RFSCPPHFTE MSPDSEPSRF SLEALTGPDT ELWLIQAPAD FAPQCLNGRR 

        70         80         90        100        110        120 
VPLSGSKTVK GKLDGKKHRY RVFTSSPQAR EATLLASSSE AGGRLTCAPA PSGSLRIMEG 

       130        140        150        160        170        180 
PQEYLLSRVP LQLIPTSLPP QIPAGLRPRF SAFGGSPPVT GPGSASALRS PTSGKRKSTR 

       190        200        210        220        230        240 
KGTDASSDTQ EAVNRHGAME VKTALGNLGV SVKKRKRYFM QEEMEAKTME PVAELPVPSA 

       250        260        270        280        290        300 
TSSKKRKKSK GTETSQVEHT EPVAQTEPPE GTFLFPTKKR KRQKEADGTE EVDGIVADSQ 

       310        320        330        340        350        360 
PQVIVEAQEE TILLSPTKKR RKEKRQNLGM EAEMGPPGVL MVTEHSEHGL QAEVALVSPK 

       370        380        390 
KTKKKKGKRV GETEAALPDD FEPQGALAPS KKKERGARL

« Hide

References

« Hide 'large scale' references
[1]"Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription."
Yamamoto K., Yamamoto M., Hanada K., Nogi Y., Matsuyama T., Muramatsu M.
Mol. Cell. Biol. 24:6338-6349(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 182-196; 218-226 AND 251-261, IDENTIFICATION IN THE RNA POL I COMPLEX, FUNCTION, INTERACTION WITH SL1 COMPLEX AND POLR1E, SUBCELLULAR LOCATION.
Tissue: Ascitic tumor.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon and Mammary tumor.
[3]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-241, MASS SPECTROMETRY.
Tissue: Embryonic brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB091121 mRNA. Translation: BAD02839.1.
BC029206 mRNA. Translation: AAH29206.1.
BC071199 mRNA. Translation: AAH71199.1.
IPIIPI00169700.
RefSeqNP_665821.1. NM_145822.2.
UniGeneMm.171370.

3D structure databases

ProteinModelPortalQ76KJ5.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000044653.

PTM databases

PhosphoSiteQ76KJ5.

Proteomic databases

PaxDbQ76KJ5.
PRIDEQ76KJ5.

Protocols and materials databases

DNASU70333.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000047036; ENSMUSP00000044653; ENSMUSG00000047649.
GeneID70333.
KEGGmmu:70333.
UCSCuc009fln.2. mouse.

Organism-specific databases

CTD10849.
MGIMGI:1917583. Cd3eap.

Phylogenomic databases

eggNOGNOG45026.
GeneTreeENSGT00450000040362.
HOGENOMHOG000120117.
HOVERGENHBG082101.
InParanoidQ76KJ5.
OMAFCAFGGN.
OrthoDBEOG4NP73W.

Gene expression databases

BgeeQ76KJ5.
GenevestigatorQ76KJ5.
GermOnlineENSMUSG00000047649. Mus musculus.

Family and domain databases

InterProIPR013240. DNA-dir_RNA_pol1_su_RPA34.
[Graphical view]
PfamPF08208. RNA_polI_A34. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio331396.
SOURCESearch...

Entry information

Entry nameRPA34_MOUSE
AccessionPrimary (citable) accession number: Q76KJ5
Secondary accession number(s): Q8K0Y9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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