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Reviewed, UniProtKB/Swiss-Prot Q76K27 (SIAT2_MOUSE)

Last modified February 9, 2010. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Beta-galactoside alpha-2,6-sialyltransferase 2
      Short name=Alpha 2,6-ST 2
    EC=2.4.99.1
Alternative name(s):
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2
    Sialyltransferase 2
    ST6Gal II
      Short name=ST6GalII
Gene names
Name: St6gal2
Synonyms: Kiaa1877
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids.

Catalytic activity

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine. Ref.1

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Mainly expressed in brain and embryo. Very low expression is also detected in spleen, oviduct, lung and skeletal muscle. Ref.1

Sequence similarities

Belongs to the glycosyltransferase 29 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q76K27-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q76K27-2)

The sequence of this isoform differs from the canonical sequence as follows:
     436-438: ILI → TCV
     439-524: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 524524Beta-galactoside alpha-2,6-sialyltransferase 2
PRO_0000314786

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3221Signal-anchor for type II membrane protein Potential
Topological domain33 – 524492Lumenal Potential
Compositional bias230 – 26233Arg-rich

Amino acid modifications

Glycosylation3021N-linked (GlcNAc...) Potential
Glycosylation3321N-linked (GlcNAc...) Potential
Glycosylation5201N-linked (GlcNAc...) Potential
Disulfide bond291 ↔ 443 By similarity

Natural variations

Alternative sequence436 – 4383ILI → TCV in isoform 2.
VSP_030357
Alternative sequence439 – 52486Missing in isoform 2.
VSP_030358

Experimental info

Sequence conflict481S → R in BAC38534. Ref.2
Sequence conflict481S → R in AAI25587. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 68F1B225FAB3287D

FASTA52460,123
        10         20         30         40         50         60 
MKPHLKQWRQ RMLFGIFVWG LLFLAIFIYF TNSNPAAPMP SSFSFLESRG LLPLQGKQRV 

        70         80         90        100        110        120 
IMGALQEPSL PRSLDASKVL LDSHPENPFH PWPGDPQKWD QAPNGFDNGD EFFTSQVGRK 

       130        140        150        160        170        180 
SQSAFYPEED SYFFVADQPE LYHHRQGALE LPSPGETSWR SGPVQPKQKL LHPRRGSLPE 

       190        200        210        220        230        240 
EAYDSDMLSA SMSRAFLYRL WKGAVSSKML NPRLQKAMRY YMSFNKHGVR FRRRGRREAT 

       250        260        270        280        290        300 
RTGPELLCEM RRRVRVRTLD GREAPFSGLG WRPLVPGVPL SQLHPRGLSS CAVVMSAGAI 

       310        320        330        340        350        360 
LNSSLGEEID SHDAVLRFNS APTRGYEKDV GNKTTVRIIN SQILANPSHH FIDSALYKDV 

       370        380        390        400        410        420 
ILVAWDPAPY SANLNLWYKK PDYNLFTPYI QHRRKYPTQP FYILHPKFIW QLWDIIQENT 

       430        440        450        460        470        480 
REKIQPNPPS SGFIGILIMM SMCKEVHVYE YIPSVRQTEL CHYHELYYDA ACTLGAYHPL 

       490        500        510        520 
LYEKLLVQRL NTGTQADLHH KGKVVLPGFQ TLRCPVTSPN NTHS 

« Hide

Isoform 2.

Checksum: 2B36EC61C71494A4
Show »

FASTA43850,213

References

« Hide 'large scale' references
[1]"Comparison of the enzymatic properties of mouse beta-galactoside alpha2,6-sialyltransferases, ST6Gal I and II."
Takashima S., Tsuji S., Tsujimoto M.
J. Biochem. 134:287-296(2003) [PubMed: 12966079] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME ACTIVITY, TISSUE SPECIFICITY.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Cerebellum.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[5]"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
DNA Res. 10:167-180(2003) [PubMed: 14621295] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 310-524 (ISOFORM 1).
Tissue: Embryonic tail.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB095093 Genomic DNA. Translation: BAC87752.1.
AK082566 mRNA. Translation: BAC38534.1.
CT009635 Genomic DNA. Translation: CAP19182.1.
BC125586 mRNA. Translation: AAI25587.1.
AK129462 mRNA. Translation: BAC98272.1. Different initiation.
IPIIPI00274908.
IPI00881028.
RefSeqNP_766417.1.
UniGeneMm.311687

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGT29. Glycosyltransferase Family 29.

Proteomic databases

PRIDEQ76K27.

Genome annotation databases

EnsemblENSMUST00000025000; ENSMUSP00000025000; ENSMUSG00000024172; Mus musculus. [Genome view]
ENSMUST00000086878; ENSMUSP00000084091; ENSMUSG00000024172; Mus musculus. [Genome view]
GeneID240119.
KEGGmmu:240119.

Organism-specific databases

CTD240119.
MGIMGI:2445190. St6gal2.
RougeSearch...

Phylogenomic databases

eggNOGroNOG05993.
HOGENOMHBG283896.
HOVERGENQ76K27.
InParanoidQ76K27.

Enzyme and pathway databases

BRENDA2.4.99.1. 244.

Gene expression databases

ArrayExpressQ76K27.
BgeeQ76K27.
CleanExMM_ST6GAL2.
GenevestigatorQ76K27.

Family and domain databases

InterProIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR001675. Glyco_trans_29.
[Graphical view]
PfamPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio384464.
SOURCESearch...

Entry information

Entry nameSIAT2_MOUSE
AccessionPrimary (citable) accession number: Q76K27
Secondary accession number(s): Q6ZPG5, Q8BUU4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 5, 2004
Last modified: February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents