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Protein

Beta-galactoside alpha-2,6-sialyltransferase 2

Gene

St6gal2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids.

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-4085001. Sialic acid metabolism.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactoside alpha-2,6-sialyltransferase 2 (EC:2.4.99.1)
Short name:
Alpha 2,6-ST 2
Alternative name(s):
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2
ST6Gal II
Short name:
ST6GalII
Sialyltransferase 2
Gene namesi
Name:St6gal2
Synonyms:Kiaa1877
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2445190. St6gal2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini33 – 524LumenalSequence analysisAdd BLAST492

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003147861 – 524Beta-galactoside alpha-2,6-sialyltransferase 2Add BLAST524

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi248 ↔ 514By similarity
Disulfide bondi291 ↔ 443By similarity
Glycosylationi302N-linked (GlcNAc...)Sequence analysis1
Glycosylationi332N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi461 ↔ 472By similarity
Glycosylationi520N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ76K27.
PRIDEiQ76K27.

PTM databases

PhosphoSitePlusiQ76K27.

Expressioni

Tissue specificityi

Mainly expressed in brain and embryo. Very low expression is also detected in spleen, oviduct, lung and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSMUSG00000024172.
CleanExiMM_ST6GAL2.
ExpressionAtlasiQ76K27. baseline and differential.
GenevisibleiQ76K27. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084091.

Structurei

3D structure databases

ProteinModelPortaliQ76K27.
SMRiQ76K27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi230 – 262Arg-richAdd BLAST33

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074444.
HOGENOMiHOG000013206.
HOVERGENiHBG052853.
InParanoidiQ76K27.
KOiK00779.
OMAiYFTDSNP.
OrthoDBiEOG091G07C7.
TreeFamiTF323961.

Family and domain databases

InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR001675. Glyco_trans_29.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
SUPFAMiSSF88713. SSF88713. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q76K27-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKPHLKQWRQ RMLFGIFVWG LLFLAIFIYF TNSNPAAPMP SSFSFLERRG
60 70 80 90 100
LLPLQGKQRV IMGALQEPSL PRSLDASKVL LDSHPENPFH PWPGDPQKWD
110 120 130 140 150
QAPNGFDNGD EFFTSQVGRK SQSAFYPEED SYFFVADQPE LYHHRQGALE
160 170 180 190 200
LPSPGETSWR SGPVQPKQKL LHPRRGSLPE EAYDSDMLSA SMSRAFLYRL
210 220 230 240 250
WKGAVSSKML NPRLQKAMRY YMSFNKHGVR FRRRGRREAT RTGPELLCEM
260 270 280 290 300
RRRVRVRTLD GREAPFSGLG WRPLVPGVPL SQLHPRGLSS CAVVMSAGAI
310 320 330 340 350
LNSSLGEEID SHDAVLRFNS APTRGYEKDV GNKTTVRIIN SQILANPSHH
360 370 380 390 400
FIDSALYKDV ILVAWDPAPY SANLNLWYKK PDYNLFTPYI QHRRKYPTQP
410 420 430 440 450
FYILHPKFIW QLWDIIQENT REKIQPNPPS SGFIGILIMM SMCKEVHVYE
460 470 480 490 500
YIPSVRQTEL CHYHELYYDA ACTLGAYHPL LYEKLLVQRL NTGTQADLHH
510 520
KGKVVLPGFQ TLRCPVTSPN NTHS
Length:524
Mass (Da):60,192
Last modified:July 27, 2011 - v2
Checksum:i9EB27CB3B901124F
GO
Isoform 2 (identifier: Q76K27-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     436-438: ILI → TCV
     439-524: Missing.

Show »
Length:438
Mass (Da):50,283
Checksum:i70189C34D6DFF59A
GO

Sequence cautioni

The sequence BAC98272 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48R → S in BAC87752 (PubMed:12966079).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030357436 – 438ILI → TCV in isoform 2. 2 Publications3
Alternative sequenceiVSP_030358439 – 524Missing in isoform 2. 2 PublicationsAdd BLAST86

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095093 Genomic DNA. Translation: BAC87752.1.
AK082566 mRNA. Translation: BAC38534.1.
CT009635 Genomic DNA. Translation: CAP19181.1.
CT009635 Genomic DNA. Translation: CAP19182.1.
BC125586 mRNA. Translation: AAI25587.1.
AK129462 mRNA. Translation: BAC98272.1. Different initiation.
CCDSiCCDS28886.1. [Q76K27-2]
RefSeqiNP_766417.1. NM_172829.2. [Q76K27-2]
XP_006524329.1. XM_006524266.2. [Q76K27-1]
XP_006524330.1. XM_006524267.2. [Q76K27-1]
XP_006524331.1. XM_006524268.2. [Q76K27-1]
XP_006524332.1. XM_006524269.2. [Q76K27-1]
UniGeneiMm.311687.

Genome annotation databases

EnsembliENSMUST00000025000; ENSMUSP00000025000; ENSMUSG00000024172. [Q76K27-1]
ENSMUST00000086878; ENSMUSP00000084091; ENSMUSG00000024172. [Q76K27-2]
ENSMUST00000183712; ENSMUSP00000139036; ENSMUSG00000098633. [Q76K27-1]
ENSMUST00000184253; ENSMUSP00000139138; ENSMUSG00000098633. [Q76K27-2]
GeneIDi240119.
KEGGimmu:240119.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST6Gal II

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095093 Genomic DNA. Translation: BAC87752.1.
AK082566 mRNA. Translation: BAC38534.1.
CT009635 Genomic DNA. Translation: CAP19181.1.
CT009635 Genomic DNA. Translation: CAP19182.1.
BC125586 mRNA. Translation: AAI25587.1.
AK129462 mRNA. Translation: BAC98272.1. Different initiation.
CCDSiCCDS28886.1. [Q76K27-2]
RefSeqiNP_766417.1. NM_172829.2. [Q76K27-2]
XP_006524329.1. XM_006524266.2. [Q76K27-1]
XP_006524330.1. XM_006524267.2. [Q76K27-1]
XP_006524331.1. XM_006524268.2. [Q76K27-1]
XP_006524332.1. XM_006524269.2. [Q76K27-1]
UniGeneiMm.311687.

3D structure databases

ProteinModelPortaliQ76K27.
SMRiQ76K27.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084091.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSitePlusiQ76K27.

Proteomic databases

PaxDbiQ76K27.
PRIDEiQ76K27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025000; ENSMUSP00000025000; ENSMUSG00000024172. [Q76K27-1]
ENSMUST00000086878; ENSMUSP00000084091; ENSMUSG00000024172. [Q76K27-2]
ENSMUST00000183712; ENSMUSP00000139036; ENSMUSG00000098633. [Q76K27-1]
ENSMUST00000184253; ENSMUSP00000139138; ENSMUSG00000098633. [Q76K27-2]
GeneIDi240119.
KEGGimmu:240119.

Organism-specific databases

CTDi84620.
MGIiMGI:2445190. St6gal2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074444.
HOGENOMiHOG000013206.
HOVERGENiHBG052853.
InParanoidiQ76K27.
KOiK00779.
OMAiYFTDSNP.
OrthoDBiEOG091G07C7.
TreeFamiTF323961.

Enzyme and pathway databases

ReactomeiR-MMU-4085001. Sialic acid metabolism.

Miscellaneous databases

PROiQ76K27.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024172.
CleanExiMM_ST6GAL2.
ExpressionAtlasiQ76K27. baseline and differential.
GenevisibleiQ76K27. MM.

Family and domain databases

InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR001675. Glyco_trans_29.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
SUPFAMiSSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIAT2_MOUSE
AccessioniPrimary (citable) accession number: Q76K27
Secondary accession number(s): A9C434, Q6ZPG5, Q8BUU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.