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Protein

N,N'-diacetylchitobiose phosphorylase

Gene

chbP

Organism
Vibrio proteolyticus (Aeromonas proteolytica)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorolysis of chitobiose (N,N'-diacetylchitobiose or (GlcNAc)2) into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration.1 Publication

Catalytic activityi

N,N'-diacetylchitobiose + phosphate = N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate.1 Publication

Kineticsi

  1. KM=2.0 mM for N,N'-diacetylchitobiose1 Publication
  2. KM=69 mM for alpha-D-glucosyl-(1-4)-2-acetamide-2-deoxy-D-glucose1 Publication
  3. KM=14 mM for N-acetyl-alpha-D-glucosamine 1-phosphate1 Publication
  4. KM=0.48 mM for alpha-D-glucose 1-phosphate1 Publication

    pH dependencei

    Optimum pH is 7.0-7.7.1 Publication

    Temperature dependencei

    Optimum temperature is 30 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi140Calcium 31 Publication1
    Metal bindingi186Calcium 11 Publication1
    Metal bindingi187Calcium 1; via carbonyl oxygen1 Publication1
    Metal bindingi190Calcium 1; via carbonyl oxygen1 Publication1
    Metal bindingi196Calcium 1; via carbonyl oxygen1 Publication1
    Binding sitei333Substrate1
    Binding sitei343Substrate1
    Active sitei489NucleophileBy similarity1
    Active sitei527Proton donorBy similarity1
    Binding sitei637Substrate1
    Binding sitei644Substrate1
    Binding sitei690Substrate1
    Metal bindingi791Calcium 2; via carbonyl oxygen1 Publication1
    Metal bindingi793Calcium 21 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BRENDAi2.4.1.280. 167.
    SABIO-RKQ76IQ9.

    Protein family/group databases

    CAZyiGH94. Glycoside Hydrolase Family 94.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N,N'-diacetylchitobiose phosphorylase (EC:2.4.1.280)
    Alternative name(s):
    Chitobiose phosphorylase
    Gene namesi
    Name:chbP
    OrganismiVibrio proteolyticus (Aeromonas proteolytica)
    Taxonomic identifieri671 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004241071 – 801N,N'-diacetylchitobiose phosphorylaseAdd BLAST801

    Proteomic databases

    PRIDEiQ76IQ9.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1801
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 7Combined sources5
    Turni8 – 11Combined sources4
    Beta strandi12 – 16Combined sources5
    Beta strandi25 – 28Combined sources4
    Beta strandi30 – 38Combined sources9
    Beta strandi41 – 48Combined sources8
    Turni50 – 52Combined sources3
    Beta strandi61 – 64Combined sources4
    Beta strandi66 – 68Combined sources3
    Beta strandi70 – 75Combined sources6
    Turni76 – 78Combined sources3
    Beta strandi81 – 86Combined sources6
    Turni87 – 90Combined sources4
    Turni93 – 95Combined sources3
    Beta strandi96 – 103Combined sources8
    Beta strandi106 – 113Combined sources8
    Beta strandi116 – 123Combined sources8
    Beta strandi128 – 139Combined sources12
    Beta strandi141 – 143Combined sources3
    Beta strandi145 – 152Combined sources8
    Helixi160 – 164Combined sources5
    Helixi167 – 170Combined sources4
    Beta strandi173 – 179Combined sources7
    Beta strandi182 – 187Combined sources6
    Turni193 – 196Combined sources4
    Beta strandi197 – 204Combined sources8
    Beta strandi207 – 212Combined sources6
    Helixi213 – 217Combined sources5
    Helixi227 – 231Combined sources5
    Beta strandi240 – 242Combined sources3
    Beta strandi244 – 254Combined sources11
    Beta strandi259 – 269Combined sources11
    Helixi272 – 279Combined sources8
    Helixi283 – 301Combined sources19
    Beta strandi304 – 307Combined sources4
    Helixi311 – 317Combined sources7
    Helixi320 – 331Combined sources12
    Beta strandi336 – 338Combined sources3
    Beta strandi345 – 347Combined sources3
    Helixi348 – 354Combined sources7
    Turni359 – 361Combined sources3
    Helixi363 – 375Combined sources13
    Beta strandi385 – 387Combined sources3
    Helixi389 – 392Combined sources4
    Helixi420 – 422Combined sources3
    Beta strandi424 – 426Combined sources3
    Helixi427 – 430Combined sources4
    Helixi431 – 442Combined sources12
    Helixi445 – 449Combined sources5
    Beta strandi451 – 453Combined sources3
    Beta strandi459 – 461Combined sources3
    Helixi462 – 475Combined sources14
    Beta strandi483 – 486Combined sources4
    Beta strandi489 – 491Combined sources3
    Beta strandi497 – 501Combined sources5
    Helixi502 – 522Combined sources21
    Helixi525 – 545Combined sources21
    Beta strandi546 – 548Combined sources3
    Turni549 – 552Combined sources4
    Beta strandi564 – 566Combined sources3
    Helixi577 – 585Combined sources9
    Helixi591 – 605Combined sources15
    Beta strandi613 – 616Combined sources4
    Turni623 – 625Combined sources3
    Helixi627 – 630Combined sources4
    Beta strandi640 – 643Combined sources4
    Helixi646 – 655Combined sources10
    Helixi659 – 669Combined sources11
    Helixi671 – 674Combined sources4
    Helixi678 – 681Combined sources4
    Beta strandi689 – 692Combined sources4
    Turni698 – 701Combined sources4
    Beta strandi703 – 708Combined sources6
    Helixi711 – 721Combined sources11
    Beta strandi726 – 728Combined sources3
    Beta strandi733 – 735Combined sources3
    Beta strandi745 – 752Combined sources8
    Beta strandi755 – 762Combined sources8
    Beta strandi771 – 776Combined sources6
    Beta strandi779 – 781Combined sources3
    Beta strandi783 – 785Combined sources3
    Beta strandi793 – 800Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1V7VX-ray1.80A1-801[»]
    1V7WX-ray1.60A1-801[»]
    1V7XX-ray2.00A1-801[»]
    ProteinModelPortaliQ76IQ9.
    SMRiQ76IQ9.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ76IQ9.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni349 – 350Substrate binding2
    Regioni490 – 492Substrate binding3
    Regioni709 – 710Substrate binding2

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 94 family.Curated

    Phylogenomic databases

    KOiK18675.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    2.70.98.40. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR009342. Carb-bd_put_dom.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR033432. GH36_catalytic.
    IPR005196. Glyco_hydro_65_N.
    IPR010383. Glyco_hydrolase_94.
    [Graphical view]
    PfamiPF17167. Glyco_hydro_36. 1 hit.
    PF06165. Glyco_transf_36. 1 hit.
    [Graphical view]
    SMARTiSM01068. CBM_X. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF74650. SSF74650. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q76IQ9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKYGYFDNDN REYVITRPDV PAPWTNYLGT EKFCTVISHN AGGYSFYNSP
    60 70 80 90 100
    EYNRVTKFRP NATFDRPGHY VYLRDDDSGD YWSISWQPVA KSLDEAQYQI
    110 120 130 140 150
    RHGLSYSKFQ CDYNGIHARK TLFVPKGEDA EIWDVVIKNT SDQVRTISAF
    160 170 180 190 200
    SFVEFSFSHI QSDNQNHQMS LYSAGTAYRP GLIEYDLYYN TDDFEGFYYL
    210 220 230 240 250
    ASTFDPDSYD GQRDRFLGLY RDEANPLAVE QGRCSNSAQT CYNHCGSLHK
    260 270 280 290 300
    QFTLQPGEEI RFAYILGIGK GNGERLREHY QDVANIDAAF AAIKAHWDER
    310 320 330 340 350
    CAKFQVKSPN QGLDTMINAW TLYQAETCVV WSRFASFIEV GGRTGLGYRD
    360 370 380 390 400
    TAQDAISVPH ANPEMTRKRI VDLLRGQVKA GYGLHLFDPD WFDPEKEDVA
    410 420 430 440 450
    PSKSPTVVPT PSDEDKIHGI KDTCSDDHLW LIPTICKYVM ETGETSFFDQ
    460 470 480 490 500
    MIPYADGGEA SVYEHMKAAL DFSAEYVGQT GICKGLRADW NDCLNLGGGE
    510 520 530 540 550
    SSMVSFLHFW ALQEFIDLAK FLGKDQDVNT YTEMAANVRE ACETHLWDDE
    560 570 580 590 600
    GGWYIRGLTK NGDKIGTAQQ QEGRVHLESN TLAVLSGLAS QERGEQAMDA
    610 620 630 640 650
    VDEHLFSPYG LHLNAPSFST PNDDIGFVTR VYQGVKENGA IFSHPNPWAW
    660 670 680 690 700
    VAETKLGRGD RAMKFYDALN PYNQNDIIEK RIAEPYSYVQ FIMGRDHQDH
    710 720 730 740 750
    GRANHPWLTG TSGWAYFAVT NYILGVQSGF TGLSVDPCIP SDWPGFEVTR
    760 770 780 790 800
    QWRGATYHIQ VENPDHVSKG VKSITLNGAP IQGRIPPQAQ GSDNQVVVVL

    G
    Length:801
    Mass (Da):90,361
    Last modified:July 5, 2004 - v1
    Checksum:iBB9D864E7FFB6EDC
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB096684 Genomic DNA. Translation: BAC87867.1.

    Genome annotation databases

    KEGGiag:BAC87867.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB096684 Genomic DNA. Translation: BAC87867.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1V7VX-ray1.80A1-801[»]
    1V7WX-ray1.60A1-801[»]
    1V7XX-ray2.00A1-801[»]
    ProteinModelPortaliQ76IQ9.
    SMRiQ76IQ9.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiGH94. Glycoside Hydrolase Family 94.

    Proteomic databases

    PRIDEiQ76IQ9.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:BAC87867.

    Phylogenomic databases

    KOiK18675.

    Enzyme and pathway databases

    BRENDAi2.4.1.280. 167.
    SABIO-RKQ76IQ9.

    Miscellaneous databases

    EvolutionaryTraceiQ76IQ9.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    2.70.98.40. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR009342. Carb-bd_put_dom.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR033432. GH36_catalytic.
    IPR005196. Glyco_hydro_65_N.
    IPR010383. Glyco_hydrolase_94.
    [Graphical view]
    PfamiPF17167. Glyco_hydro_36. 1 hit.
    PF06165. Glyco_transf_36. 1 hit.
    [Graphical view]
    SMARTiSM01068. CBM_X. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF74650. SSF74650. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCHBP_VIBPR
    AccessioniPrimary (citable) accession number: Q76IQ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: July 5, 2004
    Last modified: November 2, 2016
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.