Reviewed, UniProtKB/Swiss-Prot Q76IQ3 (TY3H_CANFA)

Names and origin

Protein names

Recommended name:
    Tyrosine 3-monooxygenase
    EC=1.14.16.2
Alternative name(s):
    Tyrosine 3-hydroxylase
      Short name=TH

Gene names

Name:

TH

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays an important role in the physiology of adrenergic neurons By similarity.
Catalytic activity	L-tyrosine + tetrahydrobiopterin + O(2) = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe(2+) ion.
Enzyme regulation	Phosphorylation leads to an increase in the catalytic activity.
Pathway	Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Ontologies

Keywords
Biological process	Catecholamine biosynthesis Neurotransmitter biosynthesis
Coding sequence diversity	Polymorphism
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	aromatic amino acid family metabolic process Inferred from electronic annotation. Source: InterPro catecholamine biosynthetic process Inferred from electronic annotation. Source: InterPro neurotransmitter biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro tyrosine 3-monooxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 495	494	Tyrosine 3-monooxygenase		PRO_0000289577
Sites
	Metal binding	328	1	Iron By similarity
	Metal binding	333	1	Iron By similarity
	Metal binding	373	1	Iron By similarity
Amino acid modifications
	Modified residue	19	1	Phosphoserine By similarity
	Modified residue	31	1	Phosphoserine By similarity
	Modified residue	40	1	Phosphoserine; by PKA By similarity
Natural variations
	Natural variant	33	1	R → C

Sequences

	Sequence		Length	Mass (Da)
	Q76IQ3-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D02E3C7784336C7B	FASTA	495	55,679
	10 20 30 40 50 60 MPTPNTASPQ AKGFRRAVSE LDAKQAEAIM SPRFIGRRQS LIEDARKERE KAEAASAASS 70 80 90 100 110 120 EPGDLLEAAV SKEKDGKAML NLLFTLRGAK TSSLSRAVKA FETFEAQIHH LETRPVQRPR 130 140 150 160 170 180 AGGPHLEYFV RCEVPSADLP ALLSSVRRVA EDVRGAGENK VLWFPRKVSE LDKCHHLVTK 190 200 210 220 230 240 FDPDLDLDHP GFSDQVYRQR RKLIAEIAFQ YKHGDPIPRV EYTAEEIATW KEVYTTLKSL 250 260 270 280 290 300 YVTHACREHL EAFQLLERFS GYREDSIPQL EDVSRFLKER TGFQLRPVAG LLSARDFLAS 310 320 330 340 350 360 LAFRVFQCTQ YIRHASSPMH SPEPDCCHEL LGHVPMLADR TFAQFSQDIG LASLGASDEE 370 380 390 400 410 420 IEKLSTLYWF TVEFGLCKQN GEVKAYGAGL LSSYGELLHS LSEEPEIRAF DPDAAAVQPY 430 440 450 460 470 480 QDQTYQSVYF VSESFSDAKD KLRNYASRIQ RPFSVKFDPY TLAIDVLDSP HAIRRSLEGV 490 QDELHTLAHA LSAIG

References

[1]

"Canine tyrosine hydroxylase (TH) gene and dopamine beta-hydroxylase (DBH) gene: their sequences, genetic polymorphisms, and diversities among five different dog breeds." Takeuchi Y., Hashizume C., Chon E.M.H., Momozawa Y., Masuda K., Kikusui T., Mori Y. J. Vet. Med. Sci. 67:861-867(2005) [PubMed: 16210796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT CYS-33. Strain: Beagle. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB097058 mRNA. Translation: BAC82588.1.
RefSeq	NP_001002966.1.
UniGene	Cfa.3446
3D structure databases
SMR	Q76IQ3. Positions 158-494.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCAFG00000010099. Canis familiaris. [Contig view]
GeneID	403444.
KEGG	cfa:403444.
Phylogenomic databases
HOVERGEN	Q76IQ3.
Family and domain databases
InterPro	IPR001273. Aaa_hydroxylase. IPR005962. Tyr_3_mOase. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF00351. Biopterin_H. 1 hit. [Graphical view]
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01269. Tyr_3_monoox. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TY3H_CANFA

Accession

Primary (citable) accession number: Q76IQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)