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Protein

Protein phosphatase Slingshot homolog 1

Gene

Ssh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.1 Publication

Miscellaneous

Tyrosine phosphatase activity has not been demonstrated for this protein to date.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei393Phosphocysteine intermediateCurated1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase Slingshot homolog 1 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
SSH-like protein 1
Short name:
SSH-1L
Short name:
mSSH-1L
Gene namesi
Name:Ssh1
Synonyms:Kiaa1298, Ssh1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2686240 Ssh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi393C → S: Abrogates phosphatase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000948422 – 1042Protein phosphatase Slingshot homolog 1Add BLAST1041

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei37PhosphoserineBy similarity1
Modified residuei57PhosphoserineBy similarity1
Modified residuei516PhosphoserineCombined sources1
Modified residuei889PhosphoserineBy similarity1
Modified residuei970PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-970 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ76I79
PRIDEiQ76I79

PTM databases

iPTMnetiQ76I79
PhosphoSitePlusiQ76I79

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney and thymus. Also expressed at lower levels in liver, skeletal muscle, small intestine and spleen.1 Publication

Developmental stagei

Ubiquitously expressed in the embryo at E14.5.1 Publication

Gene expression databases

BgeeiENSMUSG00000042121
CleanExiMM_SSH1
ExpressionAtlasiQ76I79 baseline and differential
GenevisibleiQ76I79 MM

Interactioni

Subunit structurei

Interacts with the 14-3-3 proteins YWHAB, YWHAG, YWHAQ, and YWHAZ. Interaction with 14-3-3 proteins inhibits phosphatase activity and also blocks recruitment to lamellipodia and stimulation by actin (By similarity). Interacts with actin and this stimulates phosphatase activity. Interacts with LIMK1.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231148, 2 interactors
IntActiQ76I79, 1 interactor
STRINGi10090.ENSMUSP00000124312

Structurei

3D structure databases

ProteinModelPortaliQ76I79
SMRiQ76I79
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini308 – 448Tyrosine-protein phosphataseAdd BLAST141

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni889 – 1042Interaction with YWHAGBy similarityAdd BLAST154

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118902
HOGENOMiHOG000154427
HOVERGENiHBG094001
InParanoidiQ76I79
KOiK05766
OMAiNSHCDKN
OrthoDBiEOG091G0249
PhylomeDBiQ76I79
TreeFamiTF319444

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR014876 DEK_C
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR027233 SSH1
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PTHR10159:SF138 PTHR10159:SF138, 1 hit
PfamiView protein in Pfam
PF08766 DEK_C, 1 hit
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q76I79-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVTLQRSP TPSAASSSAS NSELEAGSDE ERKLNLSLSE SFFMVKGAAL
60 70 80 90 100
FLQQGNSPQG QRSLQHPHKH AGDLPQHLQV MINLLRCEDR IKLAVRLESV
110 120 130 140 150
WTDRVRYMVV VYTSGRQDTE ENILLGVDFS SKESKSCTIG MVLRLWSDTK
160 170 180 190 200
IHLDGDGGFS VSTAGRMHIF KPVSVQAMWS ALQVLHKACE VARRHNYFPG
210 220 230 240 250
GVALIWATYY ESCISSEQSC INEWNAMQDL ESTRPDSPAL FVDKPTEGER
260 270 280 290 300
TERLIKAKLR SIMMSQDLEN VTSKEIRNEL EKQMNCNLKE FKEFIDNEML
310 320 330 340 350
LILGQMDKPS LIFDHLYLGS EWNASNLEEL QGSGVDYILN VTREIDNFFP
360 370 380 390 400
GLFAYHNIRV YDEETTDLLA HWNEAYHFIN KAKRNHSKCL VHCKMGVSRS
410 420 430 440 450
ASTVIAYAMK EFGWPLEKAY NYVKQKRSIT RPNAGFMRQL SEYEGILDAS
460 470 480 490 500
KQRHNKLWRQ QPTDDTIAEP SEFLPETLDG ALDAQLPCLD DTTHPGLPRS
510 520 530 540 550
LAPGGPALPC CFRRLSDPLL LPHHDETGGL VHLEDLEKDA LLEEEESQPV
560 570 580 590 600
EVHKLVQHPQ EGARLCEKDV KRKLEFGNSK PRSDSLPQVE ELEKDGSPRT
610 620 630 640 650
GRWRRASTQL DRSLLDQENL NNNNSKRSCP DDLERDAMFG ILSKVKPPYT
660 670 680 690 700
SCADCMYPTA GGTPEAYMER HEDPSSSAIC TQPTFLPHVT SSPMAHASSR
710 720 730 740 750
SRAPERPASG PANTSPFLLP AGSRKPDVSG SGAGAAPEPP ASLLEPSRET
760 770 780 790 800
SKALPKSLQL KNPHCDKNAA NMEVSAKEEP SPKKDPKPAK DLRLLFSNEA
810 820 830 840 850
EKPTTNSYLM QHQESIIQLQ KAGLVRKHTK ELERLKSLPS DSPAACRDSA
860 870 880 890 900
TCRLEASIPE EGSQEPAHPA LCSQAGSEEQ PVGGTLQKSP TSTLPRLDHT
910 920 930 940 950
SNFSKDFLKT VCYTPTSSSI SSNLTRSSSS DSIHSVRGKP GLVKQRAQEI
960 970 980 990 1000
ETRLRLAGLT VSSPLKRSHS LAKLGSLNFS TEDLSSEADT STIADSQDAK
1010 1020 1030 1040
CGLSSSFLPE PQSAPRDPAA TSKSSGKSAP EHLKSPSRVN KS
Length:1,042
Mass (Da):115,297
Last modified:July 5, 2004 - v1
Checksum:i19A34E4937FA48FA
GO
Isoform 2 (identifier: Q76I79-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     158-179: Missing.

Show »
Length:1,020
Mass (Da):112,878
Checksum:i553EF13BEE0F0AF6
GO

Sequence cautioni

The sequence BAD32422 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti526E → D in AAH46529 (PubMed:15489334).Curated1
Sequence conflicti550V → A in BAE41639 (PubMed:16141072).Curated1
Sequence conflicti569D → E in BAE41639 (PubMed:16141072).Curated1
Sequence conflicti867A → V in AAH46529 (PubMed:15489334).Curated1
Sequence conflicti1010E → G in BAE41639 (PubMed:16141072).Curated1
Sequence conflicti1035S → N in AAH46529 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016320158 – 179Missing in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB099287 mRNA Translation: BAC97810.1
AK173144 mRNA Translation: BAD32422.1 Different initiation.
AK155557 mRNA Translation: BAE33323.1
AK170212 mRNA Translation: BAE41639.1
AK171556 mRNA Translation: BAE42524.1
BC046529 mRNA Translation: AAH46529.1
CCDSiCCDS19556.1 [Q76I79-1]
RefSeqiNP_932777.2, NM_198109.4 [Q76I79-1]
UniGeneiMm.389682

Genome annotation databases

EnsembliENSMUST00000112298; ENSMUSP00000107917; ENSMUSG00000042121 [Q76I79-2]
ENSMUST00000159592; ENSMUSP00000124312; ENSMUSG00000042121 [Q76I79-1]
GeneIDi231637
KEGGimmu:231637
UCSCiuc008yyx.1 mouse [Q76I79-1]
uc008yyz.1 mouse [Q76I79-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSSH1_MOUSE
AccessioniPrimary (citable) accession number: Q76I79
Secondary accession number(s): Q3TDG3, Q69ZM4, Q811E5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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