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Protein

Protein phosphatase Slingshot homolog 2

Gene

SSH2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei392Phosphocysteine intermediatePROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • DNA binding Source: InterPro
  • phosphoprotein phosphatase activity Source: UniProtKB
  • protein tyrosine/serine/threonine phosphatase activity Source: InterPro
  • protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  • actin cytoskeleton organization Source: UniProtKB
  • protein dephosphorylation Source: UniProtKB
  • regulation of actin polymerization or depolymerization Source: GO_Central
  • regulation of axonogenesis Source: GO_Central
  • regulation of lamellipodium assembly Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciZFISH:HS06812-MONOMER.
SIGNORiQ76I76.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase Slingshot homolog 2 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
SSH-like protein 2
Short name:
SSH-2L
Short name:
hSSH-2L
Gene namesi
Name:SSH2
Synonyms:KIAA1725, SSH2L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:30580. SSH2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytoskeleton Source: UniProtKB-SubCell
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi392C → S: Abrogates phosphatase activity. 1 Publication1

Organism-specific databases

DisGeNETi85464.
OpenTargetsiENSG00000141298.
PharmGKBiPA134861867.

Chemistry databases

ChEMBLiCHEMBL3351198.

Polymorphism and mutation databases

BioMutaiSSH2.
DMDMi74749833.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948431 – 1423Protein phosphatase Slingshot homolog 2Add BLAST1423

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineBy similarity1
Modified residuei25PhosphoserineBy similarity1
Modified residuei36PhosphoserineCombined sources1
Modified residuei461PhosphoserineBy similarity1
Modified residuei487PhosphoserineCombined sources1
Modified residuei534PhosphoserineBy similarity1
Modified residuei631PhosphoserineBy similarity1
Modified residuei633PhosphoserineBy similarity1
Modified residuei1217PhosphoserineCombined sources1
Modified residuei1422PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ76I76.
MaxQBiQ76I76.
PaxDbiQ76I76.
PeptideAtlasiQ76I76.
PRIDEiQ76I76.

PTM databases

DEPODiQ76I76.
iPTMnetiQ76I76.
PhosphoSitePlusiQ76I76.

Expressioni

Gene expression databases

BgeeiENSG00000141298.
CleanExiHS_SSH2.
ExpressionAtlasiQ76I76. baseline and differential.
GenevisibleiQ76I76. HS.

Organism-specific databases

HPAiCAB017189.
HPA057099.

Interactioni

Subunit structurei

Interacts with filamentous actin.1 Publication

GO - Molecular functioni

  • actin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124548. 25 interactors.
IntActiQ76I76. 19 interactors.
STRINGi9606.ENSP00000269033.

Structurei

Secondary structure

11423
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi309 – 312Combined sources4
Beta strandi315 – 318Combined sources4
Helixi320 – 323Combined sources4
Helixi326 – 331Combined sources6
Beta strandi334 – 339Combined sources6
Beta strandi342 – 344Combined sources3
Beta strandi352 – 356Combined sources5
Helixi368 – 370Combined sources3
Helixi371 – 383Combined sources13
Beta strandi387 – 391Combined sources5
Beta strandi393 – 397Combined sources5
Helixi398 – 411Combined sources14
Helixi415 – 425Combined sources11
Helixi433 – 447Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NT2X-ray2.10A/B/C305-449[»]
ProteinModelPortaliQ76I76.
SMRiQ76I76.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ76I76.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini307 – 447Tyrosine-protein phosphataseAdd BLAST141

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118902.
HOVERGENiHBG094002.
InParanoidiQ76I76.
KOiK05766.
PhylomeDBiQ76I76.
TreeFamiTF319444.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di1.10.10.60. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR014876. DEK_C.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR009057. Homeodomain-like.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR027232. Ssh.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 2 hits.
PTHR10159:SF106. PTHR10159:SF106. 2 hits.
PfamiPF08766. DEK_C. 1 hit.
PF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q76I76-1) [UniParc]FASTAAdd to basket
Also known as: L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVTVQRSP TPSTTSSPCA SEADSGEEEC RSQPRSISES FLTVKGAALF
60 70 80 90 100
LPRGNGSSTP RISHRRNKHA GDLQQHLQAM FILLRPEDNI RLAVRLESTY
110 120 130 140 150
QNRTRYMVVV STNGRQDTEE SIVLGMDFSS NDSSTCTMGL VLPLWSDTLI
160 170 180 190 200
HLDGDGGFSV STDNRVHIFK PVSVQAMWSA LQSLHKACEV ARAHNYYPGS
210 220 230 240 250
LFLTWVSYYE SHINSDQSSV NEWNAMQDVQ SHRPDSPALF TDIPTERERT
260 270 280 290 300
ERLIKTKLRE IMMQKDLENI TSKEIRTELE MQMVCNLREF KEFIDNEMIV
310 320 330 340 350
ILGQMDSPTQ IFEHVFLGSE WNASNLEDLQ NRGVRYILNV TREIDNFFPG
360 370 380 390 400
VFEYHNIRVY DEEATDLLAY WNDTYKFISK AKKHGSKCLV HCKMGVSRSA
410 420 430 440 450
STVIAYAMKE YGWNLDRAYD YVKERRTVTK PNPSFMRQLE EYQGILLASK
460 470 480 490 500
QRHNKLWRSH SDSDLSDHHE PICKPGLELN KKDITTSADQ IAEVKTMESH
510 520 530 540 550
PPIPPVFVEH MVPQDANQKG LCTKERMICL EFTSREFHAG QIEDELNLND
560 570 580 590 600
INGCSSGCCL NESKFPLDNC HASKALIQPG HVPEMANKFP DLTVEDLETD
610 620 630 640 650
ALKADMNVHL LPMEELTSPL KDPPMSPDPE SPSPQPSCQT EISDFSTDRI
660 670 680 690 700
DFFSALEKFV ELSQETRSRS FSHSRMEELG GGRNESCRLS VVEVAPSKVT
710 720 730 740 750
ADDQRSSSLS NTPHASEESS MDEEQSKAIS ELVSPDIFMQ SHSENAISVK
760 770 780 790 800
EIVTEIESIS QGVGQIQLKG DILPNPCHTP KKNSIHELLL ERAQTPENKP
810 820 830 840 850
GHMEQDEDSC TAQPELAKDS GMCNPEGCLT THSSIADLEE GEPAEGEQEL
860 870 880 890 900
QGSGMHPGAK WYPGSVRRAT LEFEERLRQE QEHHGAAPTC TSLSTRKNSK
910 920 930 940 950
NDSSVADLAP KGKSDEAPPE HSFVLKEPEM SKGKGKYSGS EAGSLSHSEQ
960 970 980 990 1000
NATVPAPRVL EFDHLPDPQE GPGSDTGTQQ EGVLKDLRTV IPYQESETQA
1010 1020 1030 1040 1050
VPLPLPKRVE IIEYTHIVTS PNHTGPGSEI ATSEKSGEQG LRKVNMEKSV
1060 1070 1080 1090 1100
TVLCTLDENL NRTLDPNQVS LHPQVLPLPH SSSPEHNRPT DHPTSILSSP
1110 1120 1130 1140 1150
EDRGSSLSTA LETAAPFVSH TTHLLSASLD YLHPQTMVHL EGFTEQSSTT
1160 1170 1180 1190 1200
DEPSAEQVSW EESQESPLSS GSEVPYKDSQ LSSADLSLIS KLGDNTGELQ
1210 1220 1230 1240 1250
EKMDPLPVAC RLPHSSSSEN IKSLSHSPGV VKERAKEIES RVVFQAGLTK
1260 1270 1280 1290 1300
PSQMRRSASL AKLGYLDLCK DCLPEREPAS CESPHLKLLQ PFLRTDSGMH
1310 1320 1330 1340 1350
AMEDQESLEN PGAPHNPEPT KSFVEQLTTT ECIVQSKPVE RPLVQYAKEF
1360 1370 1380 1390 1400
GSSQQYLLPR AGLELTSSEG GLPVLQTQGL QCACPAPGLA VAPRQQHGRT
1410 1420
HPLRRLKKAN DKKRTTNPFY NTM
Length:1,423
Mass (Da):158,216
Last modified:July 5, 2004 - v1
Checksum:i23D0B59C97D6BE38
GO
Isoform 2 (identifier: Q76I76-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: MALVTVQRSP...EEECRSQPRS → MTLSTLARKR...VISQNAINQL
     179-195: SALQSLHKACEVARAHN → VDRDSRNKHCYVLLVEE
     196-1423: Missing.

Show »
Length:202
Mass (Da):22,470
Checksum:i027BBBE4C2D6FE7B
GO
Isoform 3 (identifier: Q76I76-3) [UniParc]FASTAAdd to basket
Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     179-195: SALQSLHKACEVARAHN → VDRDSRNKHCYVLLVEE
     196-1423: Missing.

Show »
Length:195
Mass (Da):21,685
Checksum:i21EB60284CB3E961
GO
Isoform 4 (identifier: Q76I76-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     449-449: S → R
     450-1423: Missing.

Note: No experimental confirmation available.
Show »
Length:449
Mass (Da):51,492
Checksum:iAFD156098A92A04D
GO

Sequence cautioni

The sequence AAH11636 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAL92027 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051758743S → L.Corresponds to variant rs2289629dbSNPEnsembl.1
Natural variantiVAR_051759763V → A.Corresponds to variant rs6505140dbSNPEnsembl.1
Natural variantiVAR_0517601300H → Q.Corresponds to variant rs8080046dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0163211 – 36MALVT…SQPRS → MTLSTLARKRKAPLACTCSL GGPDMIPYFSANAVISQNAI NQL in isoform 2. 1 PublicationAdd BLAST36
Alternative sequenceiVSP_016322179 – 195SALQS…ARAHN → VDRDSRNKHCYVLLVEE in isoform 2 and isoform 3. 2 PublicationsAdd BLAST17
Alternative sequenceiVSP_016323196 – 1423Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST1228
Alternative sequenceiVSP_016324449S → R in isoform 4. 1 Publication1
Alternative sequenceiVSP_016325450 – 1423Missing in isoform 4. 1 PublicationAdd BLAST974

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB072358 mRNA. Translation: BAB84117.1.
AB072359 mRNA. Translation: BAB84118.1.
AB099290 mRNA. Translation: BAC97813.1.
BC008941 mRNA. Translation: AAH08941.1.
BC011636 mRNA. Translation: AAH11636.2. Different initiation.
BC068223 mRNA. Translation: AAH68223.1.
AF484838 mRNA. Translation: AAL92027.1. Different initiation.
AB051512 mRNA. Translation: BAB21816.1.
CCDSiCCDS11253.1. [Q76I76-1]
RefSeqiNP_001269058.1. NM_001282129.1.
NP_001269059.1. NM_001282130.1.
NP_001269060.1. NM_001282131.1.
NP_203747.2. NM_033389.3. [Q76I76-1]
UniGeneiHs.654754.
Hs.659026.

Genome annotation databases

EnsembliENST00000269033; ENSP00000269033; ENSG00000141298. [Q76I76-1]
GeneIDi85464.
KEGGihsa:85464.
UCSCiuc002heo.3. human. [Q76I76-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB072358 mRNA. Translation: BAB84117.1.
AB072359 mRNA. Translation: BAB84118.1.
AB099290 mRNA. Translation: BAC97813.1.
BC008941 mRNA. Translation: AAH08941.1.
BC011636 mRNA. Translation: AAH11636.2. Different initiation.
BC068223 mRNA. Translation: AAH68223.1.
AF484838 mRNA. Translation: AAL92027.1. Different initiation.
AB051512 mRNA. Translation: BAB21816.1.
CCDSiCCDS11253.1. [Q76I76-1]
RefSeqiNP_001269058.1. NM_001282129.1.
NP_001269059.1. NM_001282130.1.
NP_001269060.1. NM_001282131.1.
NP_203747.2. NM_033389.3. [Q76I76-1]
UniGeneiHs.654754.
Hs.659026.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NT2X-ray2.10A/B/C305-449[»]
ProteinModelPortaliQ76I76.
SMRiQ76I76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124548. 25 interactors.
IntActiQ76I76. 19 interactors.
STRINGi9606.ENSP00000269033.

Chemistry databases

ChEMBLiCHEMBL3351198.

PTM databases

DEPODiQ76I76.
iPTMnetiQ76I76.
PhosphoSitePlusiQ76I76.

Polymorphism and mutation databases

BioMutaiSSH2.
DMDMi74749833.

Proteomic databases

EPDiQ76I76.
MaxQBiQ76I76.
PaxDbiQ76I76.
PeptideAtlasiQ76I76.
PRIDEiQ76I76.

Protocols and materials databases

DNASUi85464.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000269033; ENSP00000269033; ENSG00000141298. [Q76I76-1]
GeneIDi85464.
KEGGihsa:85464.
UCSCiuc002heo.3. human. [Q76I76-1]

Organism-specific databases

CTDi85464.
DisGeNETi85464.
GeneCardsiSSH2.
HGNCiHGNC:30580. SSH2.
HPAiCAB017189.
HPA057099.
MIMi606779. gene.
neXtProtiNX_Q76I76.
OpenTargetsiENSG00000141298.
PharmGKBiPA134861867.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118902.
HOVERGENiHBG094002.
InParanoidiQ76I76.
KOiK05766.
PhylomeDBiQ76I76.
TreeFamiTF319444.

Enzyme and pathway databases

BioCyciZFISH:HS06812-MONOMER.
SIGNORiQ76I76.

Miscellaneous databases

ChiTaRSiSSH2. human.
EvolutionaryTraceiQ76I76.
GeneWikiiSSH2.
GenomeRNAii85464.
PROiQ76I76.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000141298.
CleanExiHS_SSH2.
ExpressionAtlasiQ76I76. baseline and differential.
GenevisibleiQ76I76. HS.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di1.10.10.60. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR014876. DEK_C.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR009057. Homeodomain-like.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR027232. Ssh.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 2 hits.
PTHR10159:SF106. PTHR10159:SF106. 2 hits.
PfamiPF08766. DEK_C. 1 hit.
PF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSH2_HUMAN
AccessioniPrimary (citable) accession number: Q76I76
Secondary accession number(s): Q8TDB5
, Q8WYL1, Q8WYL2, Q96F40, Q96H36, Q9C0D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Tyrosine phosphatase activity has not been demonstrated for this protein to date.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.