Reviewed, UniProtKB/Swiss-Prot Q76I22 (LOX1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Lipoxygenase 1
    EC=1.13.11.12
Alternative name(s):
    9-lipoxygenase
    r9-LOX1

Gene names

Ordered Locus Names:	Os03g0699700, LOC_Os03g49260
ORF Names:	OSJNBb0017F17.10

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	863 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This lipoxygenase introduces molecular oxygen exclusively into the C-9 position of linoleic and linolenic.
Catalytic activity	Linoleate + O(2) = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	CytoplasmBy similarity.
Developmental stage	Expressed in the embryo 1 day after inbibition, and at low levels in developing seeds between 3 and 20 days after flowering.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 863	863	Lipoxygenase 1		PRO_0000220707
Regions
	Domain	32 – 158	127	PLAT
	Domain	161 – 863	703	Lipoxygenase
Sites
	Metal binding	518	1	Iron; catalytic By similarity
	Metal binding	523	1	Iron; catalytic By similarity
	Metal binding	709	1	Iron; catalytic By similarity
	Metal binding	713	1	Iron; catalytic By similarity
	Metal binding	863	1	Iron; via carboxylate; catalytic By similarity
Experimental info
	Sequence conflict	160	1	N → I in BAD02945. Ref.1
	Sequence conflict	163	1	L → P in BAD02945. Ref.1
	Sequence conflict	513	1	H → R in BAD02945. Ref.1
	Sequence conflict	527	1	E → K in BAD02945. Ref.1
	Sequence conflict	596	1	T → A in BAD02945. Ref.1
	Sequence conflict	663	1	E → A in BAD02945. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q76I22-1 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: AA8309F3DBCEC2F1	FASTA	863	97,499
	10 20 30 40 50 60 MLGGLKDKLT GKNGNKIKGL AVLMSRKLLD PRDFTASLLD NVHEVFGNSI TCQLVSATVA 70 80 90 100 110 120 DQNNEGRGIV GSEANLEQGL TDLPSVSQGE SKLTVRFNWE MDKHGVPGAI IIKNHHSTKF 130 140 150 160 170 180 FLKTITLHDV PGCDTIVFVA NSWIYPVGKY HYNRIFFANN SYLPSQMPEA LRPYREDELR 190 200 210 220 230 240 YLRGEDRQGP YQEHDRIYRY DVYNDLGEPD RDNPRPVLGG SQKHPYPRRG RTGRIPTKKD 250 260 270 280 290 300 PNSESRLSLL EQIYVPSDER FAHLKMSDFA GYSIKAIVQG ILPAIRTYVD LTPGEFDSFE 310 320 330 340 350 360 DILKLYRGGL KLPSIPALEE LRKSFPVQLI KDLLPVGGSY LLKFPKPDII KENEVAWRTD 370 380 390 400 410 420 EEFAREILAG LNPMVIRRLT EFPPKSTLDP SKYGDQTSTI TPAHIEKNLE GLSVQQALDS 430 440 450 460 470 480 NRLYILDHHD HFMPFLIDIN SLDGIFTYAT RTLLFLRDDD TLKPLAIELS LPHIEGNLTS 490 500 510 520 530 540 AKSKVHTPAS SGIESWVWQL AKAYVAVNDS GWHQLISHWL NTHAVMEPFV IATNRQLSVT 550 560 570 580 590 600 HPVYKLLQPH YRDTMTINAL ARQTLINGGG IFEQTVFPGK HALAMSSAVY KNWNFTEQGL 610 620 630 640 650 660 PDDLIKRGIA IKDPSSPSKV KLLIKDYPYA TDGLAIWQAI EQWVTEYCAI YYPNDGVLQG 670 680 690 700 710 720 DVELQAWWKE VREVGHGDLK DADWWPKMQS LPELTKACTT IIWIASALHA AVNFGQYPYA 730 740 750 760 770 780 GYLPNRPTIS RRPMPEPGSK EYTELDENPE KFFIRTITSQ FQTILGVSLI EILSKHSADE 790 800 810 820 830 840 IYLGQRDTPE WTSDPKALEA FKRFSRQLVE IESKVLNMNK DPLLKNRVGP ANFPYTLMFP 850 860 NTSDNKGAAE GITARGIPNS ISI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new 9-lipoxygenase cDNA from developing rice seeds." Mizuno K., Iida T., Takano A., Yokoyama M., Fujimura T. Plant Cell Physiol. 44:1168-1175(2003) [PubMed: 14634153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H.-I., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB099850 mRNA. Translation: BAD02945.1. AC097368 Genomic DNA. Translation: AAO38440.1. AC093017 Genomic DNA. Translation: AAX95631.1.
RefSeq	NP_001050990.1.
UniGene	Os.18598
3D structure databases
HSSP	HSSP built from PDB template 2SBL based on UniProtKB P08170.
ModBase	Search...
Genome annotation databases
GeneID	4333818.
KEGG	osa:4333818.
Organism-specific databases
Gramene	Q76I22.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX1_ORYSJ

Accession

Primary (citable) accession number: Q76I22
Secondary accession number(s): Q7G786

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	November 25, 2008
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)