Q76HN1 (HYAL1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronidase-1
Short name=Hyal-1
EC=3.2.1.35

Alternative name(s):
Hyaluronoglucosaminidase-1

Gene names

Name:

Hyal1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth By similarity. Overexpression of HYAL1 suppressed the growth rate of colon carcinoma cell tumors in an experimental model. Ref.2
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subcellular location	Secreted By similarity. Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Lysosome Secreted
Domain	EGF-like domain Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell death Inferred from direct assay Ref.2. Source: RGD hyaluronan catabolic process Inferred from direct assay Ref.2. Source: RGD negative regulation of cell proliferation Inferred from direct assay Ref.2. Source: RGD
Cellular component	extracellular space Traceable author statement Ref.2. Source: RGD lysosome Traceable author statement Ref.2. Source: RGD
Molecular function	hyalurononglucosaminidase activity Inferred from direct assay Ref.2. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	Potential
	Chain	40 – 449	410	Hyaluronidase-1		PRO_0000042626
Regions
	Domain	433 – 444	12	EGF-like
Sites
	Active site	146	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	231	1	N-linked (GlcNAc...) Potential
	Glycosylation	252	1	N-linked (GlcNAc...) Potential
	Glycosylation	365	1	N-linked (GlcNAc...) Potential
	Disulfide bond	58 ↔ 348		By similarity
	Disulfide bond	222 ↔ 236		By similarity
	Disulfide bond	373 ↔ 384		By similarity
	Disulfide bond	378 ↔ 433		By similarity
	Disulfide bond	435 ↔ 444		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q76HN1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EEF42E46758398EE	FASTA	449	50,645
	10 20 30 40 50 60 MKPFSPEVSP DPCPATAAHL LRTYTLFLTL LELAQGCRGS MVSNRPFITV WNADTHWCLK 70 80 90 100 110 120 DHGVDVDVSV FDVVANKEQN FQGPNMTIFY REELGTYPYY TPTGEPVFGG LPQNASLVTH 130 140 150 160 170 180 LAHAFQDIKA AMPEPDFSGL AVIDWEAWRP RWAFNWDSKD IYQQRSMELV RAEHPDWPET 190 200 210 220 230 240 LVEAEAQGQF QEAAEAWMAG TLQLGQVLRP RGLWGYYGFP DCYNYDFLSP NYTGQCSLSI 250 260 270 280 290 300 HDQNDQLGWL WNQSYALYPS IYLPAALMGT GKSQMYVRYR VQEAFRLALV SRDPHVPIMP 310 320 330 340 350 360 YVQIFYEKTD YLLPLEELEH SLGESAAQGA AGAVLWISSE KTSTKESCQA IKAYMDSTLG 370 380 390 400 410 420 PFILNVTSAA LLCSEALCSG RGRCVRHPSY PEALLTLSPA SFSIEPTHDG RPLSLKGTLS 430 440 LKDRAQMAMK FKCRCYRGWS GEWCKKQDM

References

[1]	"Expression and activity of rat hyaluronidase." Hanaki A., Ueno Y., Nakasa T., Okinaka O. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
[2]	"Expression of hyaluronan synthase 2 or hyaluronidase 1 differentially affect the growth rate of transplantable colon carcinoma cell tumors." Jacobson A., Rahmanian M., Rubin K., Heldin P. Int. J. Cancer 102:212-219(2002) [PubMed: 12397638] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB100600 mRNA. Translation: BAD14368.1.
IPI	IPI00551819.
RefSeq	NP_997499.1. NM_207616.1.
UniGene	Rn.124438.
3D structure databases
ProteinModelPortal	Q76HN1.
SMR	Q76HN1. Positions 38-449.
ModBase	Search...
Protein-protein interaction databases
STRING	Q76HN1.
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Proteomic databases
PRIDE	Q76HN1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	367166.
KEGG	rno:367166.
UCSC	NM_207616. rat.
Organism-specific databases
CTD	3373.
RGD	1303060. Hyal1.
Phylogenomic databases
eggNOG	roNOG14270.
GeneTree	ENSGT00550000074476.
HOVERGEN	HBG052053.
InParanoid	Q76HN1.
OrthoDB	EOG4VDPZD.
Gene expression databases
Genevestigator	Q76HN1.
GermOnline	ENSRNOG00000015858. Rattus norvegicus.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006210. EGF-like. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01197.
PANTHER	PTHR11769. Glyco_hydro_56. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56.
SMART	SM00181. EGF. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	690796.

Entry information

Entry name

HYAL1_RAT

Accession

Primary (citable) accession number: Q76HN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program