Q76HM9 (HYAL3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronidase-3
Short name=Hyal-3
EC=3.2.1.35

Alternative name(s):
Hyaluronoglucosaminidase-3

Gene names

Name:

Hyal3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subcellular location	Secreted By similarity. Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Lysosome Secreted
Domain	EGF-like domain Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 412	390	Hyaluronidase-3		PRO_0000248204
Regions
	Domain	353 – 408	56	EGF-like
Sites
	Active site	129	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	216	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 332		By similarity
	Disulfide bond	206 ↔ 221		By similarity
	Disulfide bond	357 ↔ 368		By similarity
	Disulfide bond	362 ↔ 396		By similarity
	Disulfide bond	398 ↔ 407		By similarity
Experimental info
	Sequence conflict	29	1	F → L in AAH97259. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q76HM9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: BB4949AADE4FB270	FASTA	412	46,207
	10 20 30 40 50 60 MITQLGLTLV VGLTLCLVHV QALLQVPEFP FSVLWNVPSA RCKTRFGVHL PLDALGIIAN 70 80 90 100 110 120 HGQRFHGQNI TIFYKNQFGL YPYFGPRGTA HNGGIPQAVS LDHHLAQAAH QILHNLGSSF 130 140 150 160 170 180 AGLAVLDWEE WYPLWAGNWG THRQVYQAAS WAWAQQMFPD LNPQEQLHKA QTGFEQAARA 190 200 210 220 230 240 LMEHTLRLGQ MLRPHGLWGF YRYPVCGNGW HNMASNYTGH CHPAIITRNT QLRWLWAASS 250 260 270 280 290 300 ALFPSIYLPP RLPPAYHQTF VRHRLEEAFR VALTGHAHPL PVLAYVRLTH RSSGRFLSLD 310 320 330 340 350 360 DLMQTIGVSA ALGAAGVVLW GDLSVSSSEE ECWRLHDYLV GTLGPYVINV TKAATACSHQ 370 380 390 400 410 RCHGHGRCSW KDPGQMEAFL HLQPDDNLGA WKSFRCRCYL GWSGPTCLEP KP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression and activity of rat hyaluronidase." Hanaki A., Ueno Y., Nakasa T., Okinaka O. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB100602 mRNA. Translation: BAD14370.1. BC097259 mRNA. Translation: AAH97259.1.
IPI	IPI00411247.
RefSeq	NP_997482.2. NM_207599.2.
UniGene	Rn.72927.
3D structure databases
HSSP	HSSP built from PDB template 1FCQ based on UniProtKB Q08169.
ProteinModelPortal	Q76HM9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q76HM9.
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Proteomic databases
PRIDE	Q76HM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000021590; ENSRNOP00000021590; ENSRNOG00000016093.
GeneID	300993.
KEGG	rno:300993.
Organism-specific databases
CTD	8372.
RGD	1303334. Hyal3.
Phylogenomic databases
GeneTree	ENSGT00550000074476.
HOVERGEN	HBG052053.
InParanoid	Q76HM9.
Gene expression databases
ArrayExpress	Q76HM9.
Genevestigator	Q76HM9.
GermOnline	ENSRNOG00000016093. Rattus norvegicus.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01197.
PANTHER	PTHR11769. Glyco_hydro_56. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56.
SMART	SM00181. EGF. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	647866.

Entry information

Entry name

HYAL3_RAT

Accession

Primary (citable) accession number: Q76HM9
Secondary accession number(s): Q4V8Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program