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Protein

Nucleolar protein 8

Gene

NOL8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in the survival of diffuse-type gastric cancer cells. Acts as a nucleolar anchoring protein for DDX47. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.3 Publications

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • DNA replication Source: HGNC
  • positive regulation of cell growth Source: HGNC
  • rRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:G66-31464-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 8
Alternative name(s):
Nucleolar protein Nop132
Gene namesi
Name:NOL8Imported
Synonyms:C9orf34, NOP132
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:23387. NOL8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nucleolus Source: UniProtKB
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55035.
OpenTargetsiENSG00000198000.
PharmGKBiPA134918056.

Polymorphism and mutation databases

BioMutaiNOL8.
DMDMi74758950.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002394431 – 1167Nucleolar protein 8Add BLAST1167

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei268PhosphoserineCombined sources1
Modified residuei298PhosphoserineCombined sources1
Modified residuei302PhosphothreonineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Modified residuei331PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei376PhosphotyrosineCombined sources1
Modified residuei378PhosphoserineCombined sources1
Modified residuei381PhosphothreonineCombined sources1
Modified residuei432PhosphoserineCombined sources1
Modified residuei723PhosphoserineCombined sources1
Modified residuei795PhosphothreonineBy similarity1
Modified residuei801PhosphoserineBy similarity1
Modified residuei837PhosphoserineCombined sources1
Modified residuei838PhosphoserineCombined sources1
Modified residuei843PhosphoserineCombined sources1
Modified residuei845PhosphoserineCombined sources1
Modified residuei888PhosphothreonineCombined sources1
Modified residuei890PhosphoserineCombined sources1
Modified residuei1036PhosphoserineCombined sources1
Cross-linki1057Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1082PhosphoserineCombined sources1
Modified residuei1083PhosphoserineCombined sources1
Modified residuei1084PhosphoserineCombined sources1
Modified residuei1099PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ76FK4.
MaxQBiQ76FK4.
PaxDbiQ76FK4.
PeptideAtlasiQ76FK4.
PRIDEiQ76FK4.

PTM databases

iPTMnetiQ76FK4.
PhosphoSitePlusiQ76FK4.

Expressioni

Tissue specificityi

Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.1 Publication

Inductioni

Up-regulated in diffuse-type gastric cancers.1 Publication

Gene expression databases

BgeeiENSG00000198000.
ExpressionAtlasiQ76FK4. baseline and differential.
GenevisibleiQ76FK4. HS.

Organism-specific databases

HPAiHPA044440.
HPA062922.

Interactioni

Subunit structurei

Interacts with the GTP form of RRAGA, RRAGC and RRAGD. Interacts with NIP7. Interacts with DDX18; the interaction is RNA-dependent. Interacts with DDX47; the interaction is RNA-dependent.2 Publications

Protein-protein interaction databases

BioGridi120364. 28 interactors.
IntActiQ76FK4. 11 interactors.
MINTiMINT-4539421.
STRINGi9606.ENSP00000401177.

Structurei

3D structure databases

ProteinModelPortaliQ76FK4.
SMRiQ76FK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 89RRMPROSITE-ProRule annotationAdd BLAST82

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili753 – 779Sequence analysisAdd BLAST27
Coiled coili886 – 924Sequence analysisAdd BLAST39

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1113 – 1116Poly-PheSequence analysis4

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4365. Eukaryota.
ENOG410Y5CN. LUCA.
GeneTreeiENSGT00390000004860.
HOVERGENiHBG057565.
InParanoidiQ76FK4.
OMAiVSKEIYY.
OrthoDBiEOG091G03DU.
PhylomeDBiQ76FK4.
TreeFamiTF323283.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 12 Publications (identifier: Q76FK4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVNRETKRL YVGGLSQDIS EADLQNQFSR FGEVSDVEII TRKDDQGNPQ
60 70 80 90 100
KVFAYINISV AEADLKKCMS VLNKTKWKGG TLQIQLAKES FLHRLAQERE
110 120 130 140 150
AAKAKKEEST TGNANLLEKT GGVDFHMKAV PGTEVPGHKN WVVSKFGRVL
160 170 180 190 200
PVLHLKNQHK RKIIKYDPSK YCHNLKKIGE DFSNTIPISS LTWELEGGND
210 220 230 240 250
PMSKKRRGEF SDFHGPPKKI IKVQKDESST GSLAMSTRPR RVIERPPLTQ
260 270 280 290 300
QQAAQKRTCD SITPSKSSPV PVSDTQKLKN LPFKTSGLET AKKRNSISDD
310 320 330 340 350
DTDSEDELRM MIAKEENLQR TTQPSINESE SDPFEVVRDD FKSGVHKLHS
360 370 380 390 400
LIGLGIKNRV SCHDSDDDIM RNDREYDSGD TDEIIAMKKN VAKVKNSTEF
410 420 430 440 450
SQMEKSTKKT SFKNRENCEL SDHCIKLQKR KSNVESALSH GLKSLNRKSP
460 470 480 490 500
SHSSSSEDAD SASELADSEG GEEYNAMMKN CLRVNLTLAD LEQLAGSDLK
510 520 530 540 550
VPNEDTKSDG PETTTQCKFD RGSKSPKTPT GLRRGRQCIR PAEIVASLLE
560 570 580 590 600
GEENTCGKQK PKENNLKPKF QAFKGVGCLY EKESMKKSLK DSVASNNKDQ
610 620 630 640 650
NSMKHEDPSI ISMEDGSPYV NGSLGEVTPC QHAKKANGPN YIQPQKRQTT
660 670 680 690 700
FESQDRKAVS PSSSEKRSKN PISRPLEGKK SLSLSAKTHN IGFDKDSCHS
710 720 730 740 750
TTKTEASQEE RSDSSGLTSL KKSPKVSSKD TREIKTDFSL SISNSSDVSA
760 770 780 790 800
KDKHAEDNEK RLAALEARQK AKEVQKKLVH NALANLDGHP EDKPTHIIFG
810 820 830 840 850
SDSECETEET STQEQSHPGE EWVKESMGKT SGKLFDSSDD DESDSEDDSN
860 870 880 890 900
RFKIKPQFEG RAGQKLMDLQ SHFGTDDRFR MDSRFLETDS EEEQEEVNEK
910 920 930 940 950
KTAEEEELAE EKKKALNVVQ SVLQINLSNS TNRGSVAAKK FKDIIHYDPT
960 970 980 990 1000
KQDHATYERK RDDKPKESKA KRKKKREEAE KLPEVSKEMY YNIAMDLKEI
1010 1020 1030 1040 1050
FQTTKYTSEK EEGTPWNEDC GKEKPEEIQD PAALTSDAEQ PSGFTFSFFD
1060 1070 1080 1090 1100
SDTKDIKEET YRVETVKPGK IVWQEDPRLQ DSSSEEEDVT EETDHRNSSP
1110 1120 1130 1140 1150
GEASLLEKET TRFFFFSKND ERLQGSDLFW RGVGSNMSRN SWEARTTNLR
1160
MDCRKKHKDA KRKMKPK
Length:1,167
Mass (Da):131,616
Last modified:July 5, 2004 - v1
Checksum:i6E64780D6F0E7415
GO
Isoform 21 Publication (identifier: Q76FK4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:1,099
Mass (Da):123,927
Checksum:iBED9401AC2D5ABE8
GO
Isoform 41 Publication (identifier: Q76FK4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     787-824: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:1,129
Mass (Da):127,337
Checksum:iCF4642596A282A1A
GO

Sequence cautioni

The sequence BAA91356 differs from that shown. Reason: Frameshift at position 1136.Curated
The sequence BAA91356 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB14229 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB15003 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69M → I in BAC99315 (PubMed:14660641).Curated1
Sequence conflicti69M → I in BAA91479 (PubMed:14702039).Curated1
Sequence conflicti454S → G in BAB14857 (PubMed:14702039).Curated1
Sequence conflicti479K → E in BAB14857 (PubMed:14702039).Curated1
Sequence conflicti630C → W in BAC99315 (PubMed:14660641).Curated1
Sequence conflicti630C → W in BAA91356 (PubMed:14702039).Curated1
Sequence conflicti733E → G in BAB14857 (PubMed:14702039).Curated1
Sequence conflicti749S → G in BAB14857 (PubMed:14702039).Curated1
Sequence conflicti945I → L in BAC99315 (PubMed:14660641).Curated1
Sequence conflicti945I → L in BAA91356 (PubMed:14702039).Curated1
Sequence conflicti966K → R in BAC99315 (PubMed:14660641).Curated1
Sequence conflicti966K → R in BAA91356 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061830470G → E.Corresponds to variant rs58545014dbSNPEnsembl.1
Natural variantiVAR_052211748V → L.Corresponds to variant rs2236344dbSNPEnsembl.1
Natural variantiVAR_052212841D → E.Corresponds to variant rs15717dbSNPEnsembl.1
Natural variantiVAR_052213988E → D.Corresponds to variant rs34224798dbSNPEnsembl.1
Natural variantiVAR_0522141021G → S.Corresponds to variant rs921122dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0520551 – 68Missing in isoform 2. 1 PublicationAdd BLAST68
Alternative sequenceiVSP_052056787 – 824Missing in isoform 4. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB105104 mRNA. Translation: BAD12268.1.
AB109030 mRNA. Translation: BAC99315.1.
AK000743 mRNA. Translation: BAA91356.1. Sequence problems.
AK001049 mRNA. Translation: BAA91479.1.
AK022755 mRNA. Translation: BAB14229.1. Different initiation.
AK024245 mRNA. Translation: BAB14857.1.
AK024786 mRNA. Translation: BAB15003.1. Different initiation.
AL136097 Genomic DNA. No translation available.
BC013788 mRNA. Translation: AAH13788.2.
CCDSiCCDS47993.1. [Q76FK4-1]
CCDS59135.1. [Q76FK4-2]
CCDS83384.1. [Q76FK4-4]
RefSeqiNP_001243323.1. NM_001256394.1. [Q76FK4-2]
NP_001317651.1. NM_001330722.1.
NP_060418.4. NM_017948.5. [Q76FK4-1]
XP_006717229.1. XM_006717166.3. [Q76FK4-1]
XP_006717230.1. XM_006717167.3. [Q76FK4-1]
XP_006717232.1. XM_006717169.3. [Q76FK4-2]
XP_006717233.1. XM_006717170.3. [Q76FK4-2]
XP_011517126.1. XM_011518824.2. [Q76FK4-2]
XP_016870365.1. XM_017014876.1. [Q76FK4-4]
UniGeneiHs.442199.

Genome annotation databases

EnsembliENST00000358855; ENSP00000351723; ENSG00000198000. [Q76FK4-2]
ENST00000442668; ENSP00000401177; ENSG00000198000. [Q76FK4-1]
ENST00000535387; ENSP00000441300; ENSG00000198000. [Q76FK4-4]
ENST00000542053; ENSP00000440709; ENSG00000198000. [Q76FK4-2]
ENST00000545558; ENSP00000441140; ENSG00000198000. [Q76FK4-1]
GeneIDi55035.
KEGGihsa:55035.
UCSCiuc064uhl.1. human. [Q76FK4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB105104 mRNA. Translation: BAD12268.1.
AB109030 mRNA. Translation: BAC99315.1.
AK000743 mRNA. Translation: BAA91356.1. Sequence problems.
AK001049 mRNA. Translation: BAA91479.1.
AK022755 mRNA. Translation: BAB14229.1. Different initiation.
AK024245 mRNA. Translation: BAB14857.1.
AK024786 mRNA. Translation: BAB15003.1. Different initiation.
AL136097 Genomic DNA. No translation available.
BC013788 mRNA. Translation: AAH13788.2.
CCDSiCCDS47993.1. [Q76FK4-1]
CCDS59135.1. [Q76FK4-2]
CCDS83384.1. [Q76FK4-4]
RefSeqiNP_001243323.1. NM_001256394.1. [Q76FK4-2]
NP_001317651.1. NM_001330722.1.
NP_060418.4. NM_017948.5. [Q76FK4-1]
XP_006717229.1. XM_006717166.3. [Q76FK4-1]
XP_006717230.1. XM_006717167.3. [Q76FK4-1]
XP_006717232.1. XM_006717169.3. [Q76FK4-2]
XP_006717233.1. XM_006717170.3. [Q76FK4-2]
XP_011517126.1. XM_011518824.2. [Q76FK4-2]
XP_016870365.1. XM_017014876.1. [Q76FK4-4]
UniGeneiHs.442199.

3D structure databases

ProteinModelPortaliQ76FK4.
SMRiQ76FK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120364. 28 interactors.
IntActiQ76FK4. 11 interactors.
MINTiMINT-4539421.
STRINGi9606.ENSP00000401177.

PTM databases

iPTMnetiQ76FK4.
PhosphoSitePlusiQ76FK4.

Polymorphism and mutation databases

BioMutaiNOL8.
DMDMi74758950.

Proteomic databases

EPDiQ76FK4.
MaxQBiQ76FK4.
PaxDbiQ76FK4.
PeptideAtlasiQ76FK4.
PRIDEiQ76FK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358855; ENSP00000351723; ENSG00000198000. [Q76FK4-2]
ENST00000442668; ENSP00000401177; ENSG00000198000. [Q76FK4-1]
ENST00000535387; ENSP00000441300; ENSG00000198000. [Q76FK4-4]
ENST00000542053; ENSP00000440709; ENSG00000198000. [Q76FK4-2]
ENST00000545558; ENSP00000441140; ENSG00000198000. [Q76FK4-1]
GeneIDi55035.
KEGGihsa:55035.
UCSCiuc064uhl.1. human. [Q76FK4-1]

Organism-specific databases

CTDi55035.
DisGeNETi55035.
GeneCardsiNOL8.
HGNCiHGNC:23387. NOL8.
HPAiHPA044440.
HPA062922.
MIMi611534. gene.
neXtProtiNX_Q76FK4.
OpenTargetsiENSG00000198000.
PharmGKBiPA134918056.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4365. Eukaryota.
ENOG410Y5CN. LUCA.
GeneTreeiENSGT00390000004860.
HOVERGENiHBG057565.
InParanoidiQ76FK4.
OMAiVSKEIYY.
OrthoDBiEOG091G03DU.
PhylomeDBiQ76FK4.
TreeFamiTF323283.

Enzyme and pathway databases

BioCyciZFISH:G66-31464-MONOMER.

Miscellaneous databases

ChiTaRSiNOL8. human.
GeneWikiiNOL8.
GenomeRNAii55035.
PROiQ76FK4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198000.
ExpressionAtlasiQ76FK4. baseline and differential.
GenevisibleiQ76FK4. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOL8_HUMAN
AccessioniPrimary (citable) accession number: Q76FK4
Secondary accession number(s): Q5TCC7
, Q5TCC8, Q5TCD3, Q5TCD5, Q5TCD6, Q5TCD7, Q76D35, Q7L3E2, Q9H586, Q9H795, Q9H7W7, Q9H9J6, Q9NWA4, Q9NWM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.