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Protein

Nucleolar protein 8

Gene

NOL8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in the survival of diffuse-type gastric cancer cells. Acts as a nucleolar anchoring protein for DDX47. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.3 Publications

GO - Molecular functioni

GO - Biological processi

  • protein localization to nucleolus Source: BHF-UCL
  • rRNA processing Source: UniProtKB

Keywordsi

Molecular functionRNA-binding
Biological processrRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 8
Alternative name(s):
Nucleolar protein Nop132
Gene namesi
Name:NOL8Imported
Synonyms:C9orf34, NOP132
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000198000.11
HGNCiHGNC:23387 NOL8
MIMi611534 gene
neXtProtiNX_Q76FK4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55035
OpenTargetsiENSG00000198000
PharmGKBiPA134918056

Polymorphism and mutation databases

BioMutaiNOL8
DMDMi74758950

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002394431 – 1167Nucleolar protein 8Add BLAST1167

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki225Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei268PhosphoserineCombined sources1
Modified residuei298PhosphoserineCombined sources1
Modified residuei302PhosphothreonineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Cross-linki314Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei331PhosphoserineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei376PhosphotyrosineCombined sources1
Modified residuei378PhosphoserineCombined sources1
Modified residuei381PhosphothreonineCombined sources1
Modified residuei432PhosphoserineCombined sources1
Modified residuei723PhosphoserineCombined sources1
Modified residuei795PhosphothreonineBy similarity1
Modified residuei801PhosphoserineBy similarity1
Modified residuei837PhosphoserineCombined sources1
Modified residuei838PhosphoserineCombined sources1
Modified residuei843PhosphoserineCombined sources1
Modified residuei845PhosphoserineCombined sources1
Modified residuei888PhosphothreonineCombined sources1
Modified residuei890PhosphoserineCombined sources1
Modified residuei1036PhosphoserineCombined sources1
Cross-linki1057Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1082PhosphoserineCombined sources1
Modified residuei1083PhosphoserineCombined sources1
Modified residuei1084PhosphoserineCombined sources1
Modified residuei1099PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ76FK4
MaxQBiQ76FK4
PaxDbiQ76FK4
PeptideAtlasiQ76FK4
PRIDEiQ76FK4

PTM databases

iPTMnetiQ76FK4
PhosphoSitePlusiQ76FK4

Expressioni

Tissue specificityi

Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.1 Publication

Inductioni

Up-regulated in diffuse-type gastric cancers.1 Publication

Gene expression databases

BgeeiENSG00000198000
ExpressionAtlasiQ76FK4 baseline and differential
GenevisibleiQ76FK4 HS

Organism-specific databases

HPAiHPA044440
HPA062922

Interactioni

Subunit structurei

Interacts with the GTP form of RRAGA, RRAGC and RRAGD. Interacts with NIP7. Interacts with DDX18; the interaction is RNA-dependent. Interacts with DDX47; the interaction is RNA-dependent.2 Publications

Protein-protein interaction databases

BioGridi120364, 32 interactors
IntActiQ76FK4, 14 interactors
MINTiQ76FK4
STRINGi9606.ENSP00000401177

Structurei

3D structure databases

ProteinModelPortaliQ76FK4
SMRiQ76FK4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 89RRMPROSITE-ProRule annotationAdd BLAST82

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili753 – 779Sequence analysisAdd BLAST27
Coiled coili886 – 924Sequence analysisAdd BLAST39

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1113 – 1116Poly-PheSequence analysis4

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4365 Eukaryota
ENOG410Y5CN LUCA
GeneTreeiENSGT00390000004860
HOVERGENiHBG057565
InParanoidiQ76FK4
OMAiHYDPTRH
OrthoDBiEOG091G03DU
PhylomeDBiQ76FK4
TreeFamiTF323283

Family and domain databases

CDDicd12226 RRM_NOL8, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR034138 NOP8_RRM
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 12 Publications (identifier: Q76FK4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVNRETKRL YVGGLSQDIS EADLQNQFSR FGEVSDVEII TRKDDQGNPQ
60 70 80 90 100
KVFAYINISV AEADLKKCMS VLNKTKWKGG TLQIQLAKES FLHRLAQERE
110 120 130 140 150
AAKAKKEEST TGNANLLEKT GGVDFHMKAV PGTEVPGHKN WVVSKFGRVL
160 170 180 190 200
PVLHLKNQHK RKIIKYDPSK YCHNLKKIGE DFSNTIPISS LTWELEGGND
210 220 230 240 250
PMSKKRRGEF SDFHGPPKKI IKVQKDESST GSLAMSTRPR RVIERPPLTQ
260 270 280 290 300
QQAAQKRTCD SITPSKSSPV PVSDTQKLKN LPFKTSGLET AKKRNSISDD
310 320 330 340 350
DTDSEDELRM MIAKEENLQR TTQPSINESE SDPFEVVRDD FKSGVHKLHS
360 370 380 390 400
LIGLGIKNRV SCHDSDDDIM RNDREYDSGD TDEIIAMKKN VAKVKNSTEF
410 420 430 440 450
SQMEKSTKKT SFKNRENCEL SDHCIKLQKR KSNVESALSH GLKSLNRKSP
460 470 480 490 500
SHSSSSEDAD SASELADSEG GEEYNAMMKN CLRVNLTLAD LEQLAGSDLK
510 520 530 540 550
VPNEDTKSDG PETTTQCKFD RGSKSPKTPT GLRRGRQCIR PAEIVASLLE
560 570 580 590 600
GEENTCGKQK PKENNLKPKF QAFKGVGCLY EKESMKKSLK DSVASNNKDQ
610 620 630 640 650
NSMKHEDPSI ISMEDGSPYV NGSLGEVTPC QHAKKANGPN YIQPQKRQTT
660 670 680 690 700
FESQDRKAVS PSSSEKRSKN PISRPLEGKK SLSLSAKTHN IGFDKDSCHS
710 720 730 740 750
TTKTEASQEE RSDSSGLTSL KKSPKVSSKD TREIKTDFSL SISNSSDVSA
760 770 780 790 800
KDKHAEDNEK RLAALEARQK AKEVQKKLVH NALANLDGHP EDKPTHIIFG
810 820 830 840 850
SDSECETEET STQEQSHPGE EWVKESMGKT SGKLFDSSDD DESDSEDDSN
860 870 880 890 900
RFKIKPQFEG RAGQKLMDLQ SHFGTDDRFR MDSRFLETDS EEEQEEVNEK
910 920 930 940 950
KTAEEEELAE EKKKALNVVQ SVLQINLSNS TNRGSVAAKK FKDIIHYDPT
960 970 980 990 1000
KQDHATYERK RDDKPKESKA KRKKKREEAE KLPEVSKEMY YNIAMDLKEI
1010 1020 1030 1040 1050
FQTTKYTSEK EEGTPWNEDC GKEKPEEIQD PAALTSDAEQ PSGFTFSFFD
1060 1070 1080 1090 1100
SDTKDIKEET YRVETVKPGK IVWQEDPRLQ DSSSEEEDVT EETDHRNSSP
1110 1120 1130 1140 1150
GEASLLEKET TRFFFFSKND ERLQGSDLFW RGVGSNMSRN SWEARTTNLR
1160
MDCRKKHKDA KRKMKPK
Length:1,167
Mass (Da):131,616
Last modified:July 5, 2004 - v1
Checksum:i6E64780D6F0E7415
GO
Isoform 21 Publication (identifier: Q76FK4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:1,099
Mass (Da):123,927
Checksum:iBED9401AC2D5ABE8
GO
Isoform 41 Publication (identifier: Q76FK4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     787-824: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:1,129
Mass (Da):127,337
Checksum:iCF4642596A282A1A
GO

Sequence cautioni

The sequence BAA91356 differs from that shown. Reason: Frameshift at position 1136.Curated
The sequence BAA91356 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB14229 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB15003 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69M → I in BAC99315 (PubMed:14660641).Curated1
Sequence conflicti69M → I in BAA91479 (PubMed:14702039).Curated1
Sequence conflicti454S → G in BAB14857 (PubMed:14702039).Curated1
Sequence conflicti479K → E in BAB14857 (PubMed:14702039).Curated1
Sequence conflicti630C → W in BAC99315 (PubMed:14660641).Curated1
Sequence conflicti630C → W in BAA91356 (PubMed:14702039).Curated1
Sequence conflicti733E → G in BAB14857 (PubMed:14702039).Curated1
Sequence conflicti749S → G in BAB14857 (PubMed:14702039).Curated1
Sequence conflicti945I → L in BAC99315 (PubMed:14660641).Curated1
Sequence conflicti945I → L in BAA91356 (PubMed:14702039).Curated1
Sequence conflicti966K → R in BAC99315 (PubMed:14660641).Curated1
Sequence conflicti966K → R in BAA91356 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061830470G → E. Corresponds to variant dbSNP:rs58545014Ensembl.1
Natural variantiVAR_052211748V → L. Corresponds to variant dbSNP:rs2236344Ensembl.1
Natural variantiVAR_052212841D → E. Corresponds to variant dbSNP:rs15717Ensembl.1
Natural variantiVAR_052213988E → D. Corresponds to variant dbSNP:rs34224798Ensembl.1
Natural variantiVAR_0522141021G → S. Corresponds to variant dbSNP:rs921122Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0520551 – 68Missing in isoform 2. 1 PublicationAdd BLAST68
Alternative sequenceiVSP_052056787 – 824Missing in isoform 4. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB105104 mRNA Translation: BAD12268.1
AB109030 mRNA Translation: BAC99315.1
AK000743 mRNA Translation: BAA91356.1 Sequence problems.
AK001049 mRNA Translation: BAA91479.1
AK022755 mRNA Translation: BAB14229.1 Different initiation.
AK024245 mRNA Translation: BAB14857.1
AK024786 mRNA Translation: BAB15003.1 Different initiation.
AL136097 Genomic DNA No translation available.
BC013788 mRNA Translation: AAH13788.2
CCDSiCCDS47993.1 [Q76FK4-1]
CCDS59135.1 [Q76FK4-2]
CCDS83384.1 [Q76FK4-4]
RefSeqiNP_001243323.1, NM_001256394.1 [Q76FK4-2]
NP_001317651.1, NM_001330722.1 [Q76FK4-4]
NP_060418.4, NM_017948.5 [Q76FK4-1]
XP_006717229.1, XM_006717166.3 [Q76FK4-1]
XP_006717230.1, XM_006717167.3 [Q76FK4-1]
XP_006717232.1, XM_006717169.3 [Q76FK4-2]
XP_006717233.1, XM_006717170.3 [Q76FK4-2]
XP_011517126.1, XM_011518824.2 [Q76FK4-2]
XP_016870365.1, XM_017014876.1 [Q76FK4-4]
UniGeneiHs.442199

Genome annotation databases

EnsembliENST00000358855; ENSP00000351723; ENSG00000198000 [Q76FK4-2]
ENST00000442668; ENSP00000401177; ENSG00000198000 [Q76FK4-1]
ENST00000535387; ENSP00000441300; ENSG00000198000 [Q76FK4-4]
ENST00000542053; ENSP00000440709; ENSG00000198000 [Q76FK4-2]
ENST00000545558; ENSP00000441140; ENSG00000198000 [Q76FK4-1]
GeneIDi55035
KEGGihsa:55035
UCSCiuc064uhl.1 human [Q76FK4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNOL8_HUMAN
AccessioniPrimary (citable) accession number: Q76FK4
Secondary accession number(s): Q5TCC7
, Q5TCC8, Q5TCD3, Q5TCD5, Q5TCD6, Q5TCD7, Q76D35, Q7L3E2, Q9H586, Q9H795, Q9H7W7, Q9H9J6, Q9NWA4, Q9NWM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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