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Q76EZ2 (UB2SC_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 S-C

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein S-C
Ubiquitin-protein ligase S-C
Gene names
Name:ube2s-c
Synonyms:ube2s.2
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme ube2c/ubch10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q76EZ2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q76EZ2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 211211Ubiquitin-conjugating enzyme E2 S-C
PRO_0000390433

Sites

Active site951Glycyl thioester intermediate By similarity

Natural variations

Alternative sequence1 – 6161Missing in isoform 2.
VSP_038532

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C0EC23D41938D5E9

FASTA21123,338
        10         20         30         40         50         60 
MNSNVENLPP HIIRRVYKEV STLTSDPPEG IKIIPNEEDI TDVQVHIEGP EGTPYAAGIF 

        70         80         90        100        110        120 
RMKLILGKDF PAAPPKGYFL TKIFHPNVSN NGEICVNVLK KDWKAELGIR HVLLTIKCLL 

       130        140        150        160        170        180 
IHPNPESALN EEAGRLLLEN YEEYASRAKL MTEIHAQGST LRGKDPTDPC SSASATVVSG 

       190        200        210 
DGPMAKKHAG DRDKKLAAKK KTDKKRALRR L 

« Hide

Isoform 2 [UniParc].

Checksum: 3E0E554674590951
Show »

FASTA15016,557

References

« Hide 'large scale' references
[1]"Molecular cloning of cDNAs encoding Xenopus ubiquitin-conjugating enzyme(E2) and their expression in various Xenopus tissues."
Karasawa A., Matushita H., Yagura T.
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Oocyte.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Gastrula.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB105547 mRNA. Translation: BAD06216.1.
BC169403 mRNA. Translation: AAI69403.1.
BC169405 mRNA. Translation: AAI69405.1.
BC085030 mRNA. Translation: AAH85030.1.
RefSeqNP_001084432.1. NM_001090963.1. [Q76EZ2-1]
UniGeneXl.6787.

3D structure databases

ProteinModelPortalQ76EZ2.
SMRQ76EZ2. Positions 6-156.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID403383.
KEGGxla:403383.

Organism-specific databases

CTD403383.

Phylogenomic databases

HOVERGENHBG063308.
KOK10583.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUB2SC_XENLA
AccessionPrimary (citable) accession number: Q76EZ2
Secondary accession number(s): Q5U4M8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways