Q76EZ2 (UB2SC_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 S-C
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein S-C
Ubiquitin-protein ligase S-C

Gene names

Name:	ube2s-c
Synonyms:	ube2s.2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme ube2c/ubch10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological_process	activation of anaphase-promoting complex activity Inferred from sequence or structural similarity. Source: UniProtKB anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW exit from mitosis Inferred from sequence or structural similarity. Source: UniProtKB free ubiquitin chain polymerization Inferred from sequence or structural similarity. Source: UniProtKB protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	anaphase-promoting complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q76EZ2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q76EZ2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Ubiquitin-conjugating enzyme E2 S-C		PRO_0000390433
Sites
	Active site	95	1	Glycyl thioester intermediate By similarity
Natural variations
	Alternative sequence	1 – 61	61	Missing in isoform 2.		VSP_038532

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C0EC23D41938D5E9	FASTA	211	23,338
	10 20 30 40 50 60 MNSNVENLPP HIIRRVYKEV STLTSDPPEG IKIIPNEEDI TDVQVHIEGP EGTPYAAGIF 70 80 90 100 110 120 RMKLILGKDF PAAPPKGYFL TKIFHPNVSN NGEICVNVLK KDWKAELGIR HVLLTIKCLL 130 140 150 160 170 180 IHPNPESALN EEAGRLLLEN YEEYASRAKL MTEIHAQGST LRGKDPTDPC SSASATVVSG 190 200 210 DGPMAKKHAG DRDKKLAAKK KTDKKRALRR L
	Isoform 2 [UniParc]. Checksum: 3E0E554674590951	FASTA	150	16,557
	10 20 30 40 50 60 MKLILGKDFP AAPPKGYFLT KIFHPNVSNN GEICVNVLKK DWKAELGIRH VLLTIKCLLI 70 80 90 100 110 120 HPNPESALNE EAGRLLLENY EEYASRAKLM TEIHAQGSTL RGKDPTDPCS SASATVVSGD 130 140 150 GPMAKKHAGD RDKKLAAKKK TDKKRALRRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of cDNAs encoding Xenopus ubiquitin-conjugating enzyme(E2) and their expression in various Xenopus tissues." Karasawa A., Matushita H., Yagura T. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Oocyte.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Gastrula.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB105547 mRNA. Translation: BAD06216.1. BC169403 mRNA. Translation: AAI69403.1. BC169405 mRNA. Translation: AAI69405.1. BC085030 mRNA. Translation: AAH85030.1.
RefSeq	NP_001084432.1. NM_001090963.1.
UniGene	Xl.6787.
3D structure databases
HSSP	HSSP built from PDB template 1AYZ based on UniProtKB P06104.
ProteinModelPortal	Q76EZ2.
SMR	Q76EZ2. Positions 6-156.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	403383.
KEGG	xla:403383.
Organism-specific databases
CTD	403383.
Phylogenomic databases
HOVERGEN	HBG063308.
KO	K10583.
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UB2SC_XENLA

Accession

Primary (citable) accession number: Q76EZ2
Secondary accession number(s): Q5U4M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2009
Last sequence update:	July 5, 2004
Last modified:	March 6, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program