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Protein

UDP-N-acetylglucosamine/UDP-glucose/GDP-mannose transporter

Gene

SLC35D2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antiporter transporting nucleotide sugars such as UDP-N-acetylglucosamine (UDP-GlcNAc), UDP-glucose (UDP-Glc) and GDP-mannose (GDP-Man) pooled in the cytosol into the lumen of the Golgi in exchange for the corresponding nucleosides monophosphates (UMP for UDP-sugars and GMP for GDP-sugars). May take part in heparan sulfate synthesis by supplying UDP-Glc-NAc, the donor substrate, and thus be involved in growth factor signaling.2 Publications

Kineticsi

  1. KM=8 µM for UDP-N-acetylglucosamine1 Publication
  2. KM=2.1 µM for UDP-glucose1 Publication
  3. KM=0.14 µM for GDP-mannose1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Biological processi

    Sugar transport, Transport

    Enzyme and pathway databases

    ReactomeiR-HSA-2022854. Keratan sulfate biosynthesis.
    R-HSA-2022928. HS-GAG biosynthesis.
    R-HSA-727802. Transport of nucleotide sugars.

    Protein family/group databases

    TCDBi2.A.7.15.1. the drug/metabolite transporter (dmt) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylglucosamine/UDP-glucose/GDP-mannose transporter
    Alternative name(s):
    Homolog of Fringe connection protein 1
    Short name:
    HFRC1
    SQV7-like protein
    Short name:
    SQV7L
    Solute carrier family 35 member D2
    UDP-galactose transporter-related protein 8
    Short name:
    UGTrel8
    Gene namesi
    Name:SLC35D2
    Synonyms:HFRC, UGTREL8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:20799. SLC35D2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2727CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei28 – 4821HelicalSequence analysisAdd
    BLAST
    Topological domaini49 – 535ExtracellularSequence analysis
    Transmembranei54 – 7421HelicalSequence analysisAdd
    BLAST
    Topological domaini75 – 14672CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei147 – 16721HelicalSequence analysisAdd
    BLAST
    Transmembranei168 – 18821HelicalSequence analysisAdd
    BLAST
    Topological domaini189 – 20113CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei202 – 22221HelicalSequence analysisAdd
    BLAST
    Topological domaini223 – 23715ExtracellularSequence analysisAdd
    BLAST
    Transmembranei238 – 25821HelicalSequence analysisAdd
    BLAST
    Topological domaini259 – 2657CytoplasmicSequence analysis
    Transmembranei266 – 28823HelicalSequence analysisAdd
    BLAST
    Topological domaini289 – 2924ExtracellularSequence analysis
    Transmembranei293 – 31523HelicalSequence analysisAdd
    BLAST
    Topological domaini316 – 33722CytoplasmicSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134909604.

    Polymorphism and mutation databases

    BioMutaiSLC35D2.
    DMDMi74749832.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337UDP-N-acetylglucosamine/UDP-glucose/GDP-mannose transporterPRO_0000313080Add
    BLAST

    Proteomic databases

    MaxQBiQ76EJ3.
    PaxDbiQ76EJ3.
    PeptideAtlasiQ76EJ3.
    PRIDEiQ76EJ3.

    Expressioni

    Tissue specificityi

    Highly expressed in heart, kidney, small intestine, placenta, lung and peripheral blood leukocyte. Weakly expressed in skeletal muscle and spleen. Not expressed in brain, colon and thymus.1 Publication

    Gene expression databases

    BgeeiQ76EJ3.
    CleanExiHS_SLC35D2.
    ExpressionAtlasiQ76EJ3. baseline and differential.
    GenevisibleiQ76EJ3. HS.

    Organism-specific databases

    HPAiHPA064047.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000253270.

    Structurei

    3D structure databases

    ProteinModelPortaliQ76EJ3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1444. Eukaryota.
    COG5070. LUCA.
    GeneTreeiENSGT00390000014204.
    HOGENOMiHOG000038339.
    HOVERGENiHBG054071.
    InParanoidiQ76EJ3.
    KOiK15281.
    OMAiTYGFPSP.
    OrthoDBiEOG76DTT0.
    PhylomeDBiQ76EJ3.
    TreeFamiTF313307.

    Family and domain databases

    InterProiIPR004853. Sugar_P_trans_dom.
    [Graphical view]
    PfamiPF03151. TPT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q76EJ3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MTAGGQAEAE GAGGEPGAAR LPSRVARLLS ALFYGTCSFL IVLVNKALLT
    60 70 80 90 100
    TYGFPSPIFL GIGQMAATIM ILYVSKLNKI IHFPDFDKKI PVKLFPLPLL
    110 120 130 140 150
    YVGNHISGLS STSKLSLPMF TVLRKFTIPL TLLLETIILG KQYSLNIILS
    160 170 180 190 200
    VFAIILGAFI AAGSDLAFNL EGYIFVFLND IFTAANGVYT KQKMDPKELG
    210 220 230 240 250
    KYGVLFYNAC FMIIPTLIIS VSTGDLQQAT EFNQWKNVVF ILQFLLSCFL
    260 270 280 290 300
    GFLLMYSTVL CSYYNSALTT AVVGAIKNVS VAYIGILIGG DYIFSLLNFV
    310 320 330
    GLNICMAGGL RYSFLTLSSQ LKPKPVGEEN ICLDLKS
    Length:337
    Mass (Da):36,673
    Last modified:July 5, 2004 - v1
    Checksum:i7895A9153A82BE5A
    GO
    Isoform 2 (identifier: Q76EJ3-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         164-251: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:249
    Mass (Da):26,702
    Checksum:i6A7E4EDF143D3609
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti184 – 1841A → S.2 Publications
    Corresponds to variant rs1051763 [ dbSNP | Ensembl ].
    VAR_037653

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei164 – 25188Missing in isoform 2. 1 PublicationVSP_030006Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB106537 mRNA. Translation: BAD14396.1.
    AB122077 mRNA. Translation: BAC99016.1.
    AL160269 Genomic DNA. Translation: CAH71614.1.
    CH471174 Genomic DNA. Translation: EAW92646.1.
    BC009413 mRNA. Translation: AAH09413.1.
    BC100278 mRNA. Translation: AAI00279.1.
    BC113579 mRNA. Translation: AAI13580.1.
    AJ005866 mRNA. Translation: CAA06743.1.
    CCDSiCCDS6717.1. [Q76EJ3-1]
    CCDS69625.1. [Q76EJ3-2]
    RefSeqiNP_001273919.1. NM_001286990.1. [Q76EJ3-2]
    NP_008932.2. NM_007001.2. [Q76EJ3-1]
    UniGeneiHs.494556.
    Hs.731624.

    Genome annotation databases

    EnsembliENST00000253270; ENSP00000253270; ENSG00000130958. [Q76EJ3-1]
    ENST00000375259; ENSP00000364408; ENSG00000130958. [Q76EJ3-2]
    GeneIDi11046.
    KEGGihsa:11046.
    UCSCiuc004awc.4. human. [Q76EJ3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB106537 mRNA. Translation: BAD14396.1.
    AB122077 mRNA. Translation: BAC99016.1.
    AL160269 Genomic DNA. Translation: CAH71614.1.
    CH471174 Genomic DNA. Translation: EAW92646.1.
    BC009413 mRNA. Translation: AAH09413.1.
    BC100278 mRNA. Translation: AAI00279.1.
    BC113579 mRNA. Translation: AAI13580.1.
    AJ005866 mRNA. Translation: CAA06743.1.
    CCDSiCCDS6717.1. [Q76EJ3-1]
    CCDS69625.1. [Q76EJ3-2]
    RefSeqiNP_001273919.1. NM_001286990.1. [Q76EJ3-2]
    NP_008932.2. NM_007001.2. [Q76EJ3-1]
    UniGeneiHs.494556.
    Hs.731624.

    3D structure databases

    ProteinModelPortaliQ76EJ3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi9606.ENSP00000253270.

    Protein family/group databases

    TCDBi2.A.7.15.1. the drug/metabolite transporter (dmt) superfamily.

    Polymorphism and mutation databases

    BioMutaiSLC35D2.
    DMDMi74749832.

    Proteomic databases

    MaxQBiQ76EJ3.
    PaxDbiQ76EJ3.
    PeptideAtlasiQ76EJ3.
    PRIDEiQ76EJ3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000253270; ENSP00000253270; ENSG00000130958. [Q76EJ3-1]
    ENST00000375259; ENSP00000364408; ENSG00000130958. [Q76EJ3-2]
    GeneIDi11046.
    KEGGihsa:11046.
    UCSCiuc004awc.4. human. [Q76EJ3-1]

    Organism-specific databases

    CTDi11046.
    GeneCardsiSLC35D2.
    HGNCiHGNC:20799. SLC35D2.
    HPAiHPA064047.
    MIMi609182. gene.
    neXtProtiNX_Q76EJ3.
    PharmGKBiPA134909604.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG1444. Eukaryota.
    COG5070. LUCA.
    GeneTreeiENSGT00390000014204.
    HOGENOMiHOG000038339.
    HOVERGENiHBG054071.
    InParanoidiQ76EJ3.
    KOiK15281.
    OMAiTYGFPSP.
    OrthoDBiEOG76DTT0.
    PhylomeDBiQ76EJ3.
    TreeFamiTF313307.

    Enzyme and pathway databases

    ReactomeiR-HSA-2022854. Keratan sulfate biosynthesis.
    R-HSA-2022928. HS-GAG biosynthesis.
    R-HSA-727802. Transport of nucleotide sugars.

    Miscellaneous databases

    ChiTaRSiSLC35D2. human.
    GenomeRNAii11046.
    PROiQ76EJ3.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ76EJ3.
    CleanExiHS_SLC35D2.
    ExpressionAtlasiQ76EJ3. baseline and differential.
    GenevisibleiQ76EJ3. HS.

    Family and domain databases

    InterProiIPR004853. Sugar_P_trans_dom.
    [Graphical view]
    PfamiPF03151. TPT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Molecular cloning and characterization of a human multisubstrate specific nucleotide-sugar transporter homologous to Drosophila fringe connection."
      Suda T., Kamiyama S., Suzuki M., Kikuchi N., Nakayama K., Narimatsu H., Jigami Y., Aoki T., Nishihara S.
      J. Biol. Chem. 279:26469-26474(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, FUNCTION.
      Tissue: Colon.
    2. "Identification and characterization of human Golgi nucleotide sugar transporter SLC35D2, a novel member of the SLC35 nucleotide sugar transporter family."
      Ishida N., Kuba T., Aoki K., Miyatake S., Kawakita M., Sanai Y.
      Genomics 85:106-116(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, VARIANT SER-184.
    3. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Lung and Skin.
    6. "Three proteins involved in Caenorhabditis elegans vulval invagination are similar to components of a glycosylation pathway."
      Herman T., Horvitz H.R.
      Proc. Natl. Acad. Sci. U.S.A. 96:974-979(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 77-337 (ISOFORM 1), VARIANT SER-184.
      Tissue: Placenta.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiS35D2_HUMAN
    AccessioniPrimary (citable) accession number: Q76EJ3
    Secondary accession number(s): O95454
    , Q498C1, Q75W21, Q7Z5X5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 5, 2004
    Last modified: July 6, 2016
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.