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Protein

Free fatty acid receptor 2

Gene

Ffar2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family (PubMed:23589301). Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation. May play a role in glucose homeostasis by regulating the secretion of GLP-1, in response to short-chain fatty acids accumulating in the intestine. May also regulate the production of LEP/Leptin, a hormone acting on the central nervous system to inhibit food intake. Finally, may also regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes. In parallel to its role in energy homeostasis, may also mediate the activation of the inflammatory and immune responses by SCFA in the intestine, regulating the rapid production of chemokines and cytokines. May also play a role in the resolution of the inflammatory response and control chemotaxis in neutrophils. In addition to SCFAs, may also be activated by the extracellular lectin FCN1 in a process leading to activation of monocytes and inducing the secretion of interleukin-8/IL-8 in response to the presence of microbes.1 Publication

GO - Molecular functioni

  • G-protein coupled receptor activity Source: UniProtKB
  • lipid binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Immunity, Inflammatory response

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-RNO-416476. G alpha (q) signalling events.
R-RNO-444209. Free fatty acid receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Free fatty acid receptor 2
Alternative name(s):
G-protein coupled receptor 43
Gene namesi
Name:Ffar2
Synonyms:Gpr43
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1359614. Ffar2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88ExtracellularSequence analysis
Transmembranei9 – 2921Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini30 – 4314CytoplasmicSequence analysisAdd
BLAST
Transmembranei44 – 6421Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini65 – 7915ExtracellularSequence analysisAdd
BLAST
Transmembranei80 – 10021Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini101 – 12626CytoplasmicSequence analysisAdd
BLAST
Transmembranei127 – 14721Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini148 – 18437ExtracellularSequence analysisAdd
BLAST
Transmembranei185 – 20521Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini206 – 21914CytoplasmicSequence analysisAdd
BLAST
Transmembranei220 – 24021Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini241 – 25515ExtracellularSequence analysisAdd
BLAST
Transmembranei256 – 27621Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini277 – 33054CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell projection Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3309100.
GuidetoPHARMACOLOGYi226.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Free fatty acid receptor 2PRO_0000228145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence analysis
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ76EI6.
PRIDEiQ76EI6.

PTM databases

PhosphoSiteiQ76EI6.

Expressioni

Tissue specificityi

Detected in whole wall and separated mucosa in the distal ileum and colon. Expressed by enteroendocrine cells expressing peptide YY (PYY) (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000021021.
GenevisibleiQ76EI6. RN.

Interactioni

Subunit structurei

Interacts with FCN1 (via Fibrinogen C-terminal domain).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028532.

Structurei

3D structure databases

ProteinModelPortaliQ76EI6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi216 – 2194Poly-Arg

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IITC. Eukaryota.
ENOG4111Q82. LUCA.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000026778.
HOVERGENiHBG051811.
InParanoidiQ76EI6.
KOiK04328.
OMAiGHCTIVI.
OrthoDBiEOG091G0EX2.
PhylomeDBiQ76EI6.
TreeFamiTF350010.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR013312. GPR40-rel_orph.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01904. GPR40FAMILY.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q76EI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPDWHSSLI LTAYILIFLT GLPANLLALR AFVSRVRQPQ PAPVHILLLN
60 70 80 90 100
LTLADLLLLL LLPFRIVEAA SNFRWYLPKI VCALTGFGFY SSIYCSTWLL
110 120 130 140 150
AGISIERYLG VAFPVQYKLS RRPLYGVIAA LVAWIMSFGH CTIVIIVQYL
160 170 180 190 200
NSTEQVGTEN QITCYENFTQ AQLDVVLPVR LELCLVLFFV PMTVTIFCYW
210 220 230 240 250
RFVWIMLTQP HVGAQRRRRA VGLAVVTLLN FLVCFGPYNM SHLVGFHLRQ
260 270 280 290 300
SPSWRVEAVV FSSLNASLDP LLFYFSSSVV RRAFGKGLLL LRNPGSSMLG
310 320 330
RGAEETVEGT KTDRGGSQTE GAQSSDFVTE
Length:330
Mass (Da):36,994
Last modified:July 5, 2004 - v1
Checksum:iCF32C5B5D226670B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB106675 mRNA. Translation: BAD02826.1.
RefSeqiNP_001005877.1. NM_001005877.1.
XP_006228883.1. XM_006228821.2.
XP_006228884.1. XM_006228822.2.
XP_008757355.1. XM_008759133.1.
XP_008757356.1. XM_008759134.1.
XP_008757357.1. XM_008759135.1.
UniGeneiRn.228916.

Genome annotation databases

EnsembliENSRNOT00000028532; ENSRNOP00000028532; ENSRNOG00000021021.
GeneIDi292794.
KEGGirno:292794.
UCSCiRGD:1359614. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB106675 mRNA. Translation: BAD02826.1.
RefSeqiNP_001005877.1. NM_001005877.1.
XP_006228883.1. XM_006228821.2.
XP_006228884.1. XM_006228822.2.
XP_008757355.1. XM_008759133.1.
XP_008757356.1. XM_008759134.1.
XP_008757357.1. XM_008759135.1.
UniGeneiRn.228916.

3D structure databases

ProteinModelPortaliQ76EI6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028532.

Chemistry

ChEMBLiCHEMBL3309100.
GuidetoPHARMACOLOGYi226.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ76EI6.

Proteomic databases

PaxDbiQ76EI6.
PRIDEiQ76EI6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028532; ENSRNOP00000028532; ENSRNOG00000021021.
GeneIDi292794.
KEGGirno:292794.
UCSCiRGD:1359614. rat.

Organism-specific databases

CTDi2867.
RGDi1359614. Ffar2.

Phylogenomic databases

eggNOGiENOG410IITC. Eukaryota.
ENOG4111Q82. LUCA.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000026778.
HOVERGENiHBG051811.
InParanoidiQ76EI6.
KOiK04328.
OMAiGHCTIVI.
OrthoDBiEOG091G0EX2.
PhylomeDBiQ76EI6.
TreeFamiTF350010.

Enzyme and pathway databases

ReactomeiR-RNO-416476. G alpha (q) signalling events.
R-RNO-444209. Free fatty acid receptors.

Miscellaneous databases

PROiQ76EI6.

Gene expression databases

BgeeiENSRNOG00000021021.
GenevisibleiQ76EI6. RN.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR013312. GPR40-rel_orph.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01904. GPR40FAMILY.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFFAR2_RAT
AccessioniPrimary (citable) accession number: Q76EI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.