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Protein

Carbohydrate sulfotransferase 9

Gene

Chst9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Participates in biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Has a higher activity toward carbonic anhydrase VI than toward lutropin. Only active against terminal GalNAcbeta1,GalNAcbeta.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi190 – 196PAPSBy similarity7
Nucleotide bindingi250 – 258PAPSBy similarity9

GO - Molecular functioni

  • N-acetylgalactosamine 4-O-sulfotransferase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 9 (EC:2.8.2.-)
Alternative name(s):
GalNAc-4-O-sulfotransferase 2
Short name:
GalNAc-4-ST2
Short name:
GalNAc4ST-2
N-acetylgalactosamine-4-O-sulfotransferase 2
Gene namesi
Name:Chst9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1918617. Chst9.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5CytoplasmicSequence analysis5
Transmembranei6 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini27 – 413LumenalSequence analysisAdd BLAST387

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001896561 – 413Carbohydrate sulfotransferase 9Add BLAST413

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi129N-linked (GlcNAc...)Sequence analysis1
Glycosylationi213N-linked (GlcNAc...)Sequence analysis1
Glycosylationi286N-linked (GlcNAc...)Sequence analysis1
Glycosylationi294N-linked (GlcNAc...)Sequence analysis1
Glycosylationi407N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ76EC5.
PRIDEiQ76EC5.

PTM databases

PhosphoSitePlusiQ76EC5.

Expressioni

Tissue specificityi

Highly expressed in kidney and pituitary gland.1 Publication

Gene expression databases

BgeeiENSMUSG00000047161.
ExpressionAtlasiQ76EC5. baseline and differential.
GenevisibleiQ76EC5. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049975.

Structurei

3D structure databases

ProteinModelPortaliQ76EC5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4651. Eukaryota.
ENOG4111GJR. LUCA.
GeneTreeiENSGT00760000119214.
HOGENOMiHOG000231801.
HOVERGENiHBG050950.
InParanoidiQ76EC5.
KOiK09673.
OMAiNAQVVRQ.
OrthoDBiEOG091G0FZO.
PhylomeDBiQ76EC5.
TreeFamiTF325581.

Family and domain databases

InterProiIPR018011. Carb_sulfotransferase-rel.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12137. PTHR12137. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q76EC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAKQVFFSV LLFGTAGLLL FMYLQAWIEE HHTGKIEKKR DQKGVSVTTG
60 70 80 90 100
KIQKQITNQN SEVHMPEDLK KKGGDLLNLG SPTRVLRKIS HSQRENGAYR
110 120 130 140 150
STEAHQGAKI EVFQKPIQMD WPLVTQPLNK SLVQGNKWKK ADATQEKRRS
160 170 180 190 200
FLHEFCKKYG RVNDPKFNLF HIVSRIYVED KHKILYCEVP KAGCSNWKRI
210 220 230 240 250
LMVLNGLASS AYNISHDTVH YGKHLKTLDS FDLKGVHMRL NTYTKAVFVR
260 270 280 290 300
DPMERLVSAF RDKFEHPNSY YHPVFGKAII KKYRPNASAE ALNNGSGVKF
310 320 330 340 350
KEFAYYLLDA HRPVGMDIHW ERVSKLCYPC LINYDFVGKF ETLGEDANYF
360 370 380 390 400
LQLIGAPKEL TFPNFKDRHS SDERTNAHVV RQYLKDLSTA ERQLIYDFYH
410
LDYLMFNYTT PHL
Length:413
Mass (Da):48,004
Last modified:July 5, 2004 - v1
Checksum:iE05D123E430DD7F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB106879 mRNA. Translation: BAC87754.1.
CCDSiCCDS29074.1.
RefSeqiNP_951010.1. NM_199055.2.
UniGeneiMm.329304.

Genome annotation databases

EnsembliENSMUST00000053017; ENSMUSP00000049975; ENSMUSG00000047161.
GeneIDi71367.
KEGGimmu:71367.
UCSCiuc008edv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB106879 mRNA. Translation: BAC87754.1.
CCDSiCCDS29074.1.
RefSeqiNP_951010.1. NM_199055.2.
UniGeneiMm.329304.

3D structure databases

ProteinModelPortaliQ76EC5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049975.

PTM databases

PhosphoSitePlusiQ76EC5.

Proteomic databases

PaxDbiQ76EC5.
PRIDEiQ76EC5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053017; ENSMUSP00000049975; ENSMUSG00000047161.
GeneIDi71367.
KEGGimmu:71367.
UCSCiuc008edv.1. mouse.

Organism-specific databases

CTDi83539.
MGIiMGI:1918617. Chst9.

Phylogenomic databases

eggNOGiKOG4651. Eukaryota.
ENOG4111GJR. LUCA.
GeneTreeiENSGT00760000119214.
HOGENOMiHOG000231801.
HOVERGENiHBG050950.
InParanoidiQ76EC5.
KOiK09673.
OMAiNAQVVRQ.
OrthoDBiEOG091G0FZO.
PhylomeDBiQ76EC5.
TreeFamiTF325581.

Enzyme and pathway databases

ReactomeiR-MMU-2022870. Chondroitin sulfate biosynthesis.

Miscellaneous databases

PROiQ76EC5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047161.
ExpressionAtlasiQ76EC5. baseline and differential.
GenevisibleiQ76EC5. MM.

Family and domain databases

InterProiIPR018011. Carb_sulfotransferase-rel.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12137. PTHR12137. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHST9_MOUSE
AccessioniPrimary (citable) accession number: Q76EC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.