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Q76BW5

- XTH8_ORYSJ

UniProt

Q76BW5 - XTH8_ORYSJ

Protein

Xyloglucan endotransglycosylase/hydrolase protein 8

Gene

XTH8

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity. May promote elongation of three internodes (II, III and IV) and may be involved in cell elongation processes.By similarity1 Publication

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei106 – 1061NucleophilePROSITE-ProRule annotation
    Active sitei110 – 1101Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BRENDAi2.4.1.207. 4460.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xyloglucan endotransglycosylase/hydrolase protein 8 (EC:2.4.1.207)
    Alternative name(s):
    End-xyloglucan transferase
    OsXRT5
    OsXTH8
    Gene namesi
    Name:XTH8
    Synonyms:XRT5
    Ordered Locus Names:Os08g0237000, LOC_Os08g13920
    ORF Names:P0682A06.17
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 8

    Organism-specific databases

    GrameneiQ76BW5.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Plants exhibit up to 50% growth reduction when they reach maturity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 25251 PublicationAdd
    BLAST
    Chaini26 – 290265Xyloglucan endotransglycosylase/hydrolase protein 8PRO_0000011836Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ76BW5.

    Expressioni

    Tissue specificityi

    Transcript strongly detected in leaf sheaths. Weakly or not expressed in leaf blades, roots and calli. Accumulation of transcript detected in shoot apex meristem, vascular tissues, young leaves, vascular bundles of leaf sheaths, and peripheral cylinder of the vascular bundles and fibers in the nodal region.1 Publication

    Inductioni

    By gibberellic acid (GA3). Accumulation continues to increase throughout 24 hours of GA3 treatment. Very little effect by other plant hormones like brassinolide (BL), 6-benzyladenine (BA), indole-3-acetic acid (IAA), and abscisic acid (ABA). Inhibitory effect from uniconazole, a potent GA biosynthesis inhibitor.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ76BW5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2 – 2625Ala-richAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    HOGENOMiHOG000236368.
    KOiK08235.
    OMAiVAWAESK.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    PRINTSiPR00737. GLHYDRLASE16.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q76BW5-1 [UniParc]FASTAAdd to Basket

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    MAKHLALSVA AAVAVSWLAA SSAAAAGFYE KFDVVGAGDH VRVVSDDGKT    50
    QQVALTLDRS SGSGFTSKDT YLFGEFSVQM KLVGGNSAGT VTSFYLSSGE 100
    GDGHDEIDIE FMGNLSGNPY VMNTNVWANG DGKKEHQFYL WFDPTADFHT 150
    YKIIWNPQNI IFQVDDVPVR TFKKYDDLAY PQSKPMRLHA TLWDGSYWAT 200
    RHGDVKIDWS GAPFVVSYRG YSTNACVNNN PAGGWSSSWC PEGTSAWIHR 250
    ELDGAELGTV AWAERNYMSY NYCADGWRFP QGFPAECYRK 290
    Length:290
    Mass (Da):32,097
    Last modified:July 5, 2004 - v1
    Checksum:i26F294BA0E0AC11B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti249 – 29042HRELD…ECYRK → KKKKTKTKTKTRTRSNYKSL PRTHQWRRI1 PublicationCuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB110604 mRNA. Translation: BAD06579.1.
    AP004705 Genomic DNA. Translation: BAD05469.1.
    PIRiJE0156.
    RefSeqiNP_001061319.1. NM_001067854.1.
    UniGeneiOs.12288.

    Genome annotation databases

    EnsemblPlantsiOS08T0237000-01; OS08T0237000-01; OS08G0237000.
    GeneIDi4345019.
    KEGGiosa:4345019.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB110604 mRNA. Translation: BAD06579.1 .
    AP004705 Genomic DNA. Translation: BAD05469.1 .
    PIRi JE0156.
    RefSeqi NP_001061319.1. NM_001067854.1.
    UniGenei Os.12288.

    3D structure databases

    ProteinModelPortali Q76BW5.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PRIDEi Q76BW5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS08T0237000-01 ; OS08T0237000-01 ; OS08G0237000 .
    GeneIDi 4345019.
    KEGGi osa:4345019.

    Organism-specific databases

    Gramenei Q76BW5.

    Phylogenomic databases

    eggNOGi COG2273.
    HOGENOMi HOG000236368.
    KOi K08235.
    OMAi VAWAESK.

    Enzyme and pathway databases

    BRENDAi 2.4.1.207. 4460.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    PRINTSi PR00737. GLHYDRLASE16.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A cDNA clone from rice accelerated overgrowth (ao) mutant encoding xyloglucanrelated protein homolog."
      Aoki T., Kameya N., Nakamura I.
      Rice Genet. Newsl. 14:133-136(1997)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Koshihikari.
    2. "Characterization of a xyloglucan endotransglucosylase gene that is up-regulated by gibberellin in rice."
      Jan A., Yang G., Nakamura H., Ichikawa H., Kitano H., Matsuoka M., Matsumoto H., Komatsu S.
      Plant Physiol. 136:3670-3681(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, TISSUE SPECIFICITY.
      Strain: cv. Ginbozu and cv. Nipponbare.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Proteomic analysis of rice leaf, stem and root tissues during growth course."
      Nozu Y., Tsugita A., Kamijo K.
      Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 26-32, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: cv. Nipponbare.
    5. "A surprising diversity and abundance of xyloglucan endotransglucosylase/hydrolases in rice. Classification and expression analysis."
      Yokoyama R., Rose J.K.C., Nishitani K.
      Plant Physiol. 134:1088-1099(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, LEVEL OF PROTEIN EXPRESSION.

    Entry informationi

    Entry nameiXTH8_ORYSJ
    AccessioniPrimary (citable) accession number: Q76BW5
    Secondary accession number(s): Q7M1Y6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Lower level of XTH8 transcript detected in Tanginbozu, a GA-deficient semidwarf mutant, and higher level detected in Slender rice 1 (slr1), a GA-insensitive mutant showing a constitutive GA-response phenotype.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3