Q76BW5 (XTH8_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xyloglucan endotransglycosylase/hydrolase protein 8
EC=2.4.1.207

Alternative name(s):
End-xyloglucan transferase
OsXRT5
OsXTH8

Gene names

Name:	XTH8
Synonyms:	XRT5
Ordered Locus Names:	Os08g0237000, LOC_Os08g13920
ORF Names:	P0682A06.17

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity. May promote elongation of three internodes (II, III and IV) and may be involved in cell elongation processes. Ref.2
Catalytic activity	Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.
Subcellular location	Secreted › cell wall Probable. Secreted › extracellular space › apoplast Probable.
Tissue specificity	Transcript strongly detected in leaf sheaths. Weakly or not expressed in leaf blades, roots and calli. Accumulation of transcript detected in shoot apex meristem, vascular tissues, young leaves, vascular bundles of leaf sheaths, and peripheral cylinder of the vascular bundles and fibers in the nodal region. Ref.2
Induction	By gibberellic acid (GA3). Accumulation continues to increase throughout 24 hours of GA3 treatment. Very little effect by other plant hormones like brassinolide (BL), 6-benzyladenine (BA), indole-3-acetic acid (IAA), and abscisic acid (ABA). Inhibitory effect from uniconazole, a potent GA biosynthesis inhibitor. Ref.2
Post-translational modification	Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.
Disruption phenotype	Plants exhibit up to 50% growth reduction when they reach maturity. Ref.2
Miscellaneous	Lower level of XTH8 transcript detected in Tanginbozu, a GA-deficient semidwarf mutant, and higher level detected in Slender rice 1 (slr1), a GA-insensitive mutant showing a constitutive GA-response phenotype.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. XTH group 2 subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular glucan metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan:xyloglucosyl transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.4
	Chain	26 – 290	265	Xyloglucan endotransglycosylase/hydrolase protein 8		PRO_0000011836
Regions
	Compositional bias	2 – 26	25	Ala-rich
Sites
	Active site	106	1	Nucleophile By similarity
	Active site	110	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	249 – 290	42	HRELD…ECYRK → KKKKTKTKTKTRTRSNYKSL PRTHQWRRI Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q76BW5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 26F294BA0E0AC11B	FASTA	290	32,097
	10 20 30 40 50 60 MAKHLALSVA AAVAVSWLAA SSAAAAGFYE KFDVVGAGDH VRVVSDDGKT QQVALTLDRS 70 80 90 100 110 120 SGSGFTSKDT YLFGEFSVQM KLVGGNSAGT VTSFYLSSGE GDGHDEIDIE FMGNLSGNPY 130 140 150 160 170 180 VMNTNVWANG DGKKEHQFYL WFDPTADFHT YKIIWNPQNI IFQVDDVPVR TFKKYDDLAY 190 200 210 220 230 240 PQSKPMRLHA TLWDGSYWAT RHGDVKIDWS GAPFVVSYRG YSTNACVNNN PAGGWSSSWC 250 260 270 280 290 PEGTSAWIHR ELDGAELGTV AWAERNYMSY NYCADGWRFP QGFPAECYRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cDNA clone from rice accelerated overgrowth (ao) mutant encoding xyloglucanrelated protein homolog." Aoki T., Kameya N., Nakamura I. Rice Genet. Newsl. 14:133-136(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Koshihikari.
[2]	"Characterization of a xyloglucan endotransglucosylase gene that is up-regulated by gibberellin in rice." Jan A., Yang G., Nakamura H., Ichikawa H., Kitano H., Matsuoka M., Matsumoto H., Komatsu S. Plant Physiol. 136:3670-3681(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, TISSUE SPECIFICITY. Strain: cv. Ginbozu and cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Proteomic analysis of rice leaf, stem and root tissues during growth course." Nozu Y., Tsugita A., Kamijo K. Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 26-32, MASS SPECTROMETRY. Strain: cv. Nipponbare.
[5]	"A surprising diversity and abundance of xyloglucan endotransglucosylase/hydrolases in rice. Classification and expression analysis." Yokoyama R., Rose J.K.C., Nishitani K. Plant Physiol. 134:1088-1099(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, LEVEL OF PROTEIN EXPRESSION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB110604 mRNA. Translation: BAD06579.1. AP004705 Genomic DNA. Translation: BAD05469.1.
PIR	JE0156.
RefSeq	NP_001061319.1. NM_001067854.1.
UniGene	Os.12288.
3D structure databases
ProteinModelPortal	Q76BW5.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Proteomic databases
PRIDE	Q76BW5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os08g13920.1; LOC_Os08g13920.1; LOC_Os08g13920.
GeneID	4345019.
KEGG	dosa:Os08t0237000-01. osa:4345019.
Organism-specific databases
Gramene	Q76BW5.
Phylogenomic databases
eggNOG	COG2273.
HOGENOM	HOG000236368.
KO	K08235.
OMA	YTIIWND.
ProtClustDB	CLSN2697196.
Enzyme and pathway databases
BRENDA	2.4.1.207. 4460.
Gene expression databases
ArrayExpress	Q76BW5.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008264. Beta_glucanase. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. IPR016455. XET. IPR010713. XET_C. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. PF06955. XET_C. 1 hit. [Graphical view]
PIRSF	PIRSF005604. XET. 1 hit.
PRINTS	PR00737. GLHYDRLASE16.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XTH8_ORYSJ

Accession

Primary (citable) accession number: Q76BW5
Secondary accession number(s): Q7M1Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program