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Protein

Xyloglucan endotransglycosylase/hydrolase protein 8

Gene

XTH8

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity). May promote elongation of three internodes (II, III and IV) and may be involved in cell elongation processes.By similarity1 Publication

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei106 – 1061NucleophilePROSITE-ProRule annotation
Active sitei110 – 1101Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BRENDAi2.4.1.207. 4460.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Xyloglucan endotransglycosylase/hydrolase protein 8 (EC:2.4.1.207)
Alternative name(s):
End-xyloglucan transferase
OsXRT5
OsXTH8
Gene namesi
Name:XTH8
Synonyms:XRT5
Ordered Locus Names:Os08g0237000, LOC_Os08g13920
ORF Names:P0682A06.17
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
ProteomesiUP000000763 Componenti: Chromosome 8

Organism-specific databases

GrameneiQ76BW5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

Plants exhibit up to 50% growth reduction when they reach maturity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 290265Xyloglucan endotransglycosylase/hydrolase protein 8PRO_0000011836Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ76BW5.

Expressioni

Tissue specificityi

Transcript strongly detected in leaf sheaths. Weakly or not expressed in leaf blades, roots and calli. Accumulation of transcript detected in shoot apex meristem, vascular tissues, young leaves, vascular bundles of leaf sheaths, and peripheral cylinder of the vascular bundles and fibers in the nodal region.1 Publication

Inductioni

By gibberellic acid (GA3). Accumulation continues to increase throughout 24 hours of GA3 treatment. Very little effect by other plant hormones like brassinolide (BL), 6-benzyladenine (BA), indole-3-acetic acid (IAA), and abscisic acid (ABA). Inhibitory effect from uniconazole, a potent GA biosynthesis inhibitor.1 Publication

Gene expression databases

ExpressionAtlasiQ76BW5. baseline.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os08g13920.1.

Structurei

3D structure databases

ProteinModelPortaliQ76BW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 2625Ala-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ76BW5.
KOiK08235.
OMAiGSDAWMS.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q76BW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKHLALSVA AAVAVSWLAA SSAAAAGFYE KFDVVGAGDH VRVVSDDGKT
60 70 80 90 100
QQVALTLDRS SGSGFTSKDT YLFGEFSVQM KLVGGNSAGT VTSFYLSSGE
110 120 130 140 150
GDGHDEIDIE FMGNLSGNPY VMNTNVWANG DGKKEHQFYL WFDPTADFHT
160 170 180 190 200
YKIIWNPQNI IFQVDDVPVR TFKKYDDLAY PQSKPMRLHA TLWDGSYWAT
210 220 230 240 250
RHGDVKIDWS GAPFVVSYRG YSTNACVNNN PAGGWSSSWC PEGTSAWIHR
260 270 280 290
ELDGAELGTV AWAERNYMSY NYCADGWRFP QGFPAECYRK
Length:290
Mass (Da):32,097
Last modified:July 5, 2004 - v1
Checksum:i26F294BA0E0AC11B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti249 – 29042HRELD…ECYRK → KKKKTKTKTKTRTRSNYKSL PRTHQWRRI (Ref. 1) CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB110604 mRNA. Translation: BAD06579.1.
AP004705 Genomic DNA. Translation: BAD05469.1.
PIRiJE0156.
RefSeqiNP_001061319.1. NM_001067854.1.
UniGeneiOs.12288.

Genome annotation databases

EnsemblPlantsiOS08T0237000-01; OS08T0237000-01; OS08G0237000.
GeneIDi4345019.
KEGGiosa:4345019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB110604 mRNA. Translation: BAD06579.1.
AP004705 Genomic DNA. Translation: BAD05469.1.
PIRiJE0156.
RefSeqiNP_001061319.1. NM_001067854.1.
UniGeneiOs.12288.

3D structure databases

ProteinModelPortaliQ76BW5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os08g13920.1.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEiQ76BW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS08T0237000-01; OS08T0237000-01; OS08G0237000.
GeneIDi4345019.
KEGGiosa:4345019.

Organism-specific databases

GrameneiQ76BW5.

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000236368.
InParanoidiQ76BW5.
KOiK08235.
OMAiGSDAWMS.

Enzyme and pathway databases

BRENDAi2.4.1.207. 4460.

Gene expression databases

ExpressionAtlasiQ76BW5. baseline.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR010713. XET_C.
IPR016455. XTH.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A cDNA clone from rice accelerated overgrowth (ao) mutant encoding xyloglucanrelated protein homolog."
    Aoki T., Kameya N., Nakamura I.
    Rice Genet. Newsl. 14:133-136(1997)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Koshihikari.
  2. "Characterization of a xyloglucan endotransglucosylase gene that is up-regulated by gibberellin in rice."
    Jan A., Yang G., Nakamura H., Ichikawa H., Kitano H., Matsuoka M., Matsumoto H., Komatsu S.
    Plant Physiol. 136:3670-3681(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, TISSUE SPECIFICITY.
    Strain: cv. Ginbozu and cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "Proteomic analysis of rice leaf, stem and root tissues during growth course."
    Nozu Y., Tsugita A., Kamijo K.
    Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 26-32, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: cv. Nipponbare.
  5. "A surprising diversity and abundance of xyloglucan endotransglucosylase/hydrolases in rice. Classification and expression analysis."
    Yokoyama R., Rose J.K.C., Nishitani K.
    Plant Physiol. 134:1088-1099(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, LEVEL OF PROTEIN EXPRESSION.

Entry informationi

Entry nameiXTH8_ORYSJ
AccessioniPrimary (citable) accession number: Q76BW5
Secondary accession number(s): Q7M1Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Lower level of XTH8 transcript detected in Tanginbozu, a GA-deficient semidwarf mutant, and higher level detected in Slender rice 1 (slr1), a GA-insensitive mutant showing a constitutive GA-response phenotype.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.