Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q76BW5 (XTH8_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Xyloglucan endotransglycosylase/hydrolase protein 8

EC=2.4.1.207
Alternative name(s):
End-xyloglucan transferase
OsXRT5
OsXTH8
Gene names
Name:XTH8
Synonyms:XRT5
Ordered Locus Names:Os08g0237000, LOC_Os08g13920
ORF Names:P0682A06.17
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity. May promote elongation of three internodes (II, III and IV) and may be involved in cell elongation processes. Ref.2

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Tissue specificity

Transcript strongly detected in leaf sheaths. Weakly or not expressed in leaf blades, roots and calli. Accumulation of transcript detected in shoot apex meristem, vascular tissues, young leaves, vascular bundles of leaf sheaths, and peripheral cylinder of the vascular bundles and fibers in the nodal region. Ref.2

Induction

By gibberellic acid (GA3). Accumulation continues to increase throughout 24 hours of GA3 treatment. Very little effect by other plant hormones like brassinolide (BL), 6-benzyladenine (BA), indole-3-acetic acid (IAA), and abscisic acid (ABA). Inhibitory effect from uniconazole, a potent GA biosynthesis inhibitor. Ref.2

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Disruption phenotype

Plants exhibit up to 50% growth reduction when they reach maturity. Ref.2

Miscellaneous

Lower level of XTH8 transcript detected in Tanginbozu, a GA-deficient semidwarf mutant, and higher level detected in Slender rice 1 (slr1), a GA-insensitive mutant showing a constitutive GA-response phenotype.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.4
Chain26 – 290265Xyloglucan endotransglycosylase/hydrolase protein 8
PRO_0000011836

Regions

Compositional bias2 – 2625Ala-rich

Sites

Active site1061Nucleophile By similarity
Active site1101Proton donor By similarity

Amino acid modifications

Glycosylation1141N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict249 – 29042HRELD…ECYRK → KKKKTKTKTKTRTRSNYKSL PRTHQWRRI Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q76BW5 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 26F294BA0E0AC11B

FASTA29032,097
        10         20         30         40         50         60 
MAKHLALSVA AAVAVSWLAA SSAAAAGFYE KFDVVGAGDH VRVVSDDGKT QQVALTLDRS 

        70         80         90        100        110        120 
SGSGFTSKDT YLFGEFSVQM KLVGGNSAGT VTSFYLSSGE GDGHDEIDIE FMGNLSGNPY 

       130        140        150        160        170        180 
VMNTNVWANG DGKKEHQFYL WFDPTADFHT YKIIWNPQNI IFQVDDVPVR TFKKYDDLAY 

       190        200        210        220        230        240 
PQSKPMRLHA TLWDGSYWAT RHGDVKIDWS GAPFVVSYRG YSTNACVNNN PAGGWSSSWC 

       250        260        270        280        290 
PEGTSAWIHR ELDGAELGTV AWAERNYMSY NYCADGWRFP QGFPAECYRK 

« Hide

References

« Hide 'large scale' references
[1]"A cDNA clone from rice accelerated overgrowth (ao) mutant encoding xyloglucanrelated protein homolog."
Aoki T., Kameya N., Nakamura I.
Rice Genet. Newsl. 14:133-136(1997)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Koshihikari.
[2]"Characterization of a xyloglucan endotransglucosylase gene that is up-regulated by gibberellin in rice."
Jan A., Yang G., Nakamura H., Ichikawa H., Kitano H., Matsuoka M., Matsumoto H., Komatsu S.
Plant Physiol. 136:3670-3681(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, TISSUE SPECIFICITY.
Strain: cv. Ginbozu and cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"Proteomic analysis of rice leaf, stem and root tissues during growth course."
Nozu Y., Tsugita A., Kamijo K.
Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 26-32, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: cv. Nipponbare.
[5]"A surprising diversity and abundance of xyloglucan endotransglucosylase/hydrolases in rice. Classification and expression analysis."
Yokoyama R., Rose J.K.C., Nishitani K.
Plant Physiol. 134:1088-1099(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, LEVEL OF PROTEIN EXPRESSION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB110604 mRNA. Translation: BAD06579.1.
AP004705 Genomic DNA. Translation: BAD05469.1.
PIRJE0156.
RefSeqNP_001061319.1. NM_001067854.1.
UniGeneOs.12288.

3D structure databases

ProteinModelPortalQ76BW5.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEQ76BW5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS08T0237000-01; OS08T0237000-01; OS08G0237000.
GeneID4345019.
KEGGosa:4345019.

Organism-specific databases

GrameneQ76BW5.

Phylogenomic databases

eggNOGCOG2273.
HOGENOMHOG000236368.
KOK08235.
OMAVAWAESK.

Enzyme and pathway databases

BRENDA2.4.1.207. 4460.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
PRINTSPR00737. GLHYDRLASE16.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH8_ORYSJ
AccessionPrimary (citable) accession number: Q76BW5
Secondary accession number(s): Q7M1Y6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries