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Q76BK2 (MIF_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Macrophage migration inhibitory factor

Short name=MIF
EC=5.3.2.1
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase
EC=5.3.3.12
Phenylpyruvate tautomerase
Gene names
Name:mif
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length115 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense By similarity. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. Required for normal neural development during embryogenesis. Ref.1

Catalytic activity

Keto-phenylpyruvate = enol-phenylpyruvate.

L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Subunit structure

Homotrimer. Ref.1

Subcellular location

Secreted By similarity. Cytoplasm By similarity. Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation By similarity.

Tissue specificity

Ubiquitous. Detected throughout embryogenesis. Detected in adult spleen, liver, kidney, heart, brain, intestine, testis and lung. Ref.1

Sequence similarities

Belongs to the MIF family.

Ontologies

Keywords
   Biological processImmunity
Inflammatory response
Innate immunity
   Cellular componentCytoplasm
Secreted
   Molecular functionCytokine
Isomerase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processinflammatory response

Inferred from electronic annotation. Source: UniProtKB-KW

innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiondopachrome isomerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

phenylpyruvate tautomerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 115114Macrophage migration inhibitory factor
PRO_0000344370

Sites

Active site21Proton acceptor; via imino nitrogen By similarity
Binding site331Substrate By similarity
Binding site651Substrate; via amide nitrogen By similarity
Binding site981Substrate By similarity

Secondary structure

....................... 115
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q76BK2 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: FE04E099E97B52BB

FASTA11512,500
        10         20         30         40         50         60 
MPVFTIRTNV CRDSVPDTLL SDLTKQLAKA TGKPAEYIAI HIVPDQIMSF GDSTDPCAVC 

        70         80         90        100        110 
SLCSIGKIGG PQNKSYTKLL CDILTKQLNI PANRVYINYY DLNAANVGWN GSTFA 

« Hide

References

« Hide 'large scale' references
[1]"Xenopus laevis macrophage migration inhibitory factor is essential for axis formation and neural development."
Suzuki M., Takamura Y., Maeno M., Tochinai S., Iyaguchi D., Tanaka I., Nishihira J., Ishibashi T.
J. Biol. Chem. 279:21406-21414(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
Tissue: Liver.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB111063 mRNA. Translation: BAD02463.1.
BC097727 mRNA. Translation: AAH97727.1.
RefSeqNP_001083650.1. NM_001090181.1.
UniGeneXl.30778.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UIZX-ray2.50A/B/C/D1-115[»]
ProteinModelPortalQ76BK2.
SMRQ76BK2. Positions 1-115.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ76BK2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID399041.
KEGGxla:399041.

Organism-specific databases

CTD4282.
XenbaseXB-GENE-487452. mif.

Phylogenomic databases

HOVERGENHBG003240.
KOK07253.

Family and domain databases

InterProIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERPTHR11954. PTHR11954. 1 hit.
PfamPF01187. MIF. 1 hit.
[Graphical view]
ProDomPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF55331. SSF55331. 1 hit.
PROSITEPS01158. MIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ76BK2.

Entry information

Entry nameMIF_XENLA
AccessionPrimary (citable) accession number: Q76BK2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references