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Q76BK2

- MIF_XENLA

UniProt

Q76BK2 - MIF_XENLA

Protein

Macrophage migration inhibitory factor

Gene

mif

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense By similarity. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. Required for normal neural development during embryogenesis.By similarity1 Publication

    Catalytic activityi

    Keto-phenylpyruvate = enol-phenylpyruvate.
    L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Proton acceptor; via imino nitrogenBy similarity
    Binding sitei33 – 331SubstrateBy similarity
    Binding sitei65 – 651Substrate; via amide nitrogenBy similarity
    Binding sitei98 – 981SubstrateBy similarity

    GO - Molecular functioni

    1. dopachrome isomerase activity Source: UniProtKB-EC
    2. phenylpyruvate tautomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. inflammatory response Source: UniProtKB-KW
    2. innate immune response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Cytokine, Isomerase

    Keywords - Biological processi

    Immunity, Inflammatory response, Innate immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Macrophage migration inhibitory factor (EC:5.3.2.1)
    Short name:
    MIF
    Alternative name(s):
    L-dopachrome isomerase
    L-dopachrome tautomerase (EC:5.3.3.12)
    Phenylpyruvate tautomerase
    Gene namesi
    Name:mif
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-487452. mif.

    Subcellular locationi

    Secreted By similarity. Cytoplasm By similarity
    Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular space Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 115114Macrophage migration inhibitory factorPRO_0000344370Add
    BLAST

    Proteomic databases

    PRIDEiQ76BK2.

    Expressioni

    Tissue specificityi

    Ubiquitous. Detected throughout embryogenesis. Detected in adult spleen, liver, kidney, heart, brain, intestine, testis and lung.1 Publication

    Interactioni

    Subunit structurei

    Homotrimer.1 Publication

    Structurei

    Secondary structure

    1
    115
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 108
    Helixi12 – 143
    Helixi19 – 3113
    Helixi35 – 373
    Beta strandi39 – 435
    Beta strandi47 – 504
    Beta strandi58 – 669
    Helixi70 – 8819
    Helixi92 – 943
    Beta strandi95 – 1017
    Helixi104 – 1063
    Beta strandi107 – 1093

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UIZX-ray2.50A/B/C/D1-115[»]
    ProteinModelPortaliQ76BK2.
    SMRiQ76BK2. Positions 1-115.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ76BK2.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MIF family.Curated

    Phylogenomic databases

    HOVERGENiHBG003240.
    KOiK07253.

    Family and domain databases

    InterProiIPR001398. Macrophage_inhib_fac.
    IPR019829. Macrophage_inhib_fac_CS.
    IPR014347. Tautomerase/MIF_sf.
    [Graphical view]
    PANTHERiPTHR11954. PTHR11954. 1 hit.
    PfamiPF01187. MIF. 1 hit.
    [Graphical view]
    ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF55331. SSF55331. 1 hit.
    PROSITEiPS01158. MIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q76BK2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVFTIRTNV CRDSVPDTLL SDLTKQLAKA TGKPAEYIAI HIVPDQIMSF    50
    GDSTDPCAVC SLCSIGKIGG PQNKSYTKLL CDILTKQLNI PANRVYINYY 100
    DLNAANVGWN GSTFA 115
    Length:115
    Mass (Da):12,500
    Last modified:July 5, 2004 - v1
    Checksum:iFE04E099E97B52BB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB111063 mRNA. Translation: BAD02463.1.
    BC097727 mRNA. Translation: AAH97727.1.
    RefSeqiNP_001083650.1. NM_001090181.1.
    UniGeneiXl.30778.

    Genome annotation databases

    GeneIDi399041.
    KEGGixla:399041.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB111063 mRNA. Translation: BAD02463.1 .
    BC097727 mRNA. Translation: AAH97727.1 .
    RefSeqi NP_001083650.1. NM_001090181.1.
    UniGenei Xl.30778.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1UIZ X-ray 2.50 A/B/C/D 1-115 [» ]
    ProteinModelPortali Q76BK2.
    SMRi Q76BK2. Positions 1-115.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q76BK2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 399041.
    KEGGi xla:399041.

    Organism-specific databases

    CTDi 4282.
    Xenbasei XB-GENE-487452. mif.

    Phylogenomic databases

    HOVERGENi HBG003240.
    KOi K07253.

    Miscellaneous databases

    EvolutionaryTracei Q76BK2.

    Family and domain databases

    InterProi IPR001398. Macrophage_inhib_fac.
    IPR019829. Macrophage_inhib_fac_CS.
    IPR014347. Tautomerase/MIF_sf.
    [Graphical view ]
    PANTHERi PTHR11954. PTHR11954. 1 hit.
    Pfami PF01187. MIF. 1 hit.
    [Graphical view ]
    ProDomi PD004816. Macrophage_inhib_fac. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF55331. SSF55331. 1 hit.
    PROSITEi PS01158. MIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Xenopus laevis macrophage migration inhibitory factor is essential for axis formation and neural development."
      Suzuki M., Takamura Y., Maeno M., Tochinai S., Iyaguchi D., Tanaka I., Nishihira J., Ishibashi T.
      J. Biol. Chem. 279:21406-21414(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION, SUBUNIT, TISSUE SPECIFICITY.
      Tissue: Liver.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo.

    Entry informationi

    Entry nameiMIF_XENLA
    AccessioniPrimary (citable) accession number: Q76BK2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3