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Protein

Rab effector Noc2

Gene

Rph3al

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rab GTPase effector involved in the late steps of regulated exocytosis, both in endocrine and exocrine cells. Regulates the exocytosis of dense-core vesicles in neuroendocrine cells through interaction with RAB27A. Acts as a potential RAB3B effector protein in epithelial cells.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri89 – 14658FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • exocytosis Source: UniProtKB-KW
  • glucose homeostasis Source: MGI
  • intracellular protein transport Source: InterPro
  • negative regulation of G-protein coupled receptor protein signaling pathway Source: MGI
  • positive regulation of protein secretion Source: Ensembl
  • regulation of calcium ion-dependent exocytosis Source: MGI
  • response to drug Source: Ensembl
  • vesicle fusion Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Rab effector Noc2
Alternative name(s):
No C2 domains protein
Rabphilin-3A-like protein
Gene namesi
Name:Rph3al
Synonyms:Gm1753, Noc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1923492. Rph3al.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi51 – 555ELEII → ALEIA: Loss of binding to RAB27A, remaining cytoplasmic. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Rab effector Noc2PRO_0000278264Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei248 – 2481PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ768S4.
PRIDEiQ768S4.

PTM databases

iPTMnetiQ768S4.
PhosphoSiteiQ768S4.

Expressioni

Tissue specificityi

Highly expressed in pancreatic islets. High to moderate expression in adrenal gland, pituitary gland and ovary.1 Publication

Gene expression databases

BgeeiQ768S4.
CleanExiMM_RPH3AL.
ExpressionAtlasiQ768S4. baseline and differential.
GenevisibleiQ768S4. MM.

Interactioni

Subunit structurei

Recruited to dense-core vesicles through specific interaction with RAB27A in endocrine cells. Interacts with RAB3A, RAB3B, RAB3C and RAB3D. Interacts with ZYX (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi237613. 7 interactions.
IntActiQ768S4. 1 interaction.
STRINGi10090.ENSMUSP00000064202.

Structurei

3D structure databases

ProteinModelPortaliQ768S4.
SMRiQ768S4. Positions 47-165.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 158118RabBDPROSITE-ProRule annotationAdd
BLAST

Domaini

The N-terminus of the RabBD domain is necessary and sufficient for interaction with RAB27A.1 Publication

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri89 – 14658FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1013. Eukaryota.
ENOG410XQXA. LUCA.
GeneTreeiENSGT00440000034248.
HOGENOMiHOG000126816.
HOVERGENiHBG052990.
InParanoidiQ768S4.
KOiK19939.
OMAiEAPRMGF.
OrthoDBiEOG7CZK67.
PhylomeDBiQ768S4.
TreeFamiTF342971.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR010911. Rab_BD.
IPR030538. Rab_effect_Noc2.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF244. PTHR10024:SF244. 1 hit.
PfamiPF02318. FYVE_2. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q768S4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADTIFSSGN DQWVCPNDRQ LALRAKLQTG WSVHTYQTEK QRRSQCLSPG
60 70 80 90 100
ELEIILQVIQ RAERLDILEQ QRIGRLVERL ETMQRNVMGN GLSQCLLCGE
110 120 130 140 150
VLGFLGSSSV FCKDCRKKVC TKCGIEASPG QKRPLWLCKI CSEQREVWKR
160 170 180 190 200
SGAWFYKGLP KYILPLKTPG RADDPHFRPL PVEPTETQPP SAETSRVYTW
210 220 230 240 250
ARGRVVSSDS DSDSDLSSSS LEDRPLPSGV KGTKGDKPRG DSGASMESPR
260 270 280 290 300
LGPARPPSHL SGSQSSLGSE AGTGATEPQG GTPAQPEPRV PGKRHTWATP

RY
Length:302
Mass (Da):33,259
Last modified:July 5, 2004 - v1
Checksum:i5AE4A68535653324
GO
Isoform 2 (identifier: Q768S4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     118-141: KVCTKCGIEASPGQKRPLWLCKIC → LGIRWLQHFLESLLILNRDLTCSL
     142-302: Missing.

Show »
Length:141
Mass (Da):16,166
Checksum:iF500312CD1913D1E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei118 – 14124KVCTK…LCKIC → LGIRWLQHFLESLLILNRDL TCSL in isoform 2. 1 PublicationVSP_023245Add
BLAST
Alternative sequencei142 – 302161Missing in isoform 2. 1 PublicationVSP_023246Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB112925 mRNA. Translation: BAD07030.1.
AB158403 mRNA. Translation: BAD18853.1.
AK018335 mRNA. Translation: BAB31169.1.
AL731710 Genomic DNA. Translation: CAI24681.1.
AL731710, AL669897 Genomic DNA. Translation: CAI24682.1.
AL669897, AL731710 Genomic DNA. Translation: CAI26031.1.
BC127960 mRNA. Translation: AAI27961.1.
CCDSiCCDS25058.1. [Q768S4-1]
CCDS70243.1. [Q768S4-2]
RefSeqiNP_001278088.1. NM_001291159.1. [Q768S4-2]
NP_083824.1. NM_029548.4. [Q768S4-1]
XP_006533700.1. XM_006533637.1. [Q768S4-1]
XP_006533701.1. XM_006533638.2. [Q768S4-1]
XP_006533702.1. XM_006533639.2. [Q768S4-1]
XP_006533703.1. XM_006533640.2. [Q768S4-1]
XP_006533704.1. XM_006533641.2. [Q768S4-1]
XP_006533705.1. XM_006533642.2. [Q768S4-1]
XP_011247403.1. XM_011249101.1. [Q768S4-2]
XP_011247404.1. XM_011249102.1. [Q768S4-2]
UniGeneiMm.425697.
Mm.488178.

Genome annotation databases

EnsembliENSMUST00000021208; ENSMUSP00000021208; ENSMUSG00000020847. [Q768S4-2]
ENSMUST00000066504; ENSMUSP00000064202; ENSMUSG00000020847. [Q768S4-1]
ENSMUST00000108420; ENSMUSP00000104058; ENSMUSG00000020847. [Q768S4-2]
ENSMUST00000121287; ENSMUSP00000113869; ENSMUSG00000020847. [Q768S4-1]
GeneIDi380714.
KEGGimmu:380714.
UCSCiuc007kew.2. mouse. [Q768S4-1]
uc007kex.2. mouse. [Q768S4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB112925 mRNA. Translation: BAD07030.1.
AB158403 mRNA. Translation: BAD18853.1.
AK018335 mRNA. Translation: BAB31169.1.
AL731710 Genomic DNA. Translation: CAI24681.1.
AL731710, AL669897 Genomic DNA. Translation: CAI24682.1.
AL669897, AL731710 Genomic DNA. Translation: CAI26031.1.
BC127960 mRNA. Translation: AAI27961.1.
CCDSiCCDS25058.1. [Q768S4-1]
CCDS70243.1. [Q768S4-2]
RefSeqiNP_001278088.1. NM_001291159.1. [Q768S4-2]
NP_083824.1. NM_029548.4. [Q768S4-1]
XP_006533700.1. XM_006533637.1. [Q768S4-1]
XP_006533701.1. XM_006533638.2. [Q768S4-1]
XP_006533702.1. XM_006533639.2. [Q768S4-1]
XP_006533703.1. XM_006533640.2. [Q768S4-1]
XP_006533704.1. XM_006533641.2. [Q768S4-1]
XP_006533705.1. XM_006533642.2. [Q768S4-1]
XP_011247403.1. XM_011249101.1. [Q768S4-2]
XP_011247404.1. XM_011249102.1. [Q768S4-2]
UniGeneiMm.425697.
Mm.488178.

3D structure databases

ProteinModelPortaliQ768S4.
SMRiQ768S4. Positions 47-165.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi237613. 7 interactions.
IntActiQ768S4. 1 interaction.
STRINGi10090.ENSMUSP00000064202.

PTM databases

iPTMnetiQ768S4.
PhosphoSiteiQ768S4.

Proteomic databases

PaxDbiQ768S4.
PRIDEiQ768S4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021208; ENSMUSP00000021208; ENSMUSG00000020847. [Q768S4-2]
ENSMUST00000066504; ENSMUSP00000064202; ENSMUSG00000020847. [Q768S4-1]
ENSMUST00000108420; ENSMUSP00000104058; ENSMUSG00000020847. [Q768S4-2]
ENSMUST00000121287; ENSMUSP00000113869; ENSMUSG00000020847. [Q768S4-1]
GeneIDi380714.
KEGGimmu:380714.
UCSCiuc007kew.2. mouse. [Q768S4-1]
uc007kex.2. mouse. [Q768S4-2]

Organism-specific databases

CTDi9501.
MGIiMGI:1923492. Rph3al.

Phylogenomic databases

eggNOGiKOG1013. Eukaryota.
ENOG410XQXA. LUCA.
GeneTreeiENSGT00440000034248.
HOGENOMiHOG000126816.
HOVERGENiHBG052990.
InParanoidiQ768S4.
KOiK19939.
OMAiEAPRMGF.
OrthoDBiEOG7CZK67.
PhylomeDBiQ768S4.
TreeFamiTF342971.

Miscellaneous databases

PROiQ768S4.
SOURCEiSearch...

Gene expression databases

BgeeiQ768S4.
CleanExiMM_RPH3AL.
ExpressionAtlasiQ768S4. baseline and differential.
GenevisibleiQ768S4. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR010911. Rab_BD.
IPR030538. Rab_effect_Noc2.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10024:SF244. PTHR10024:SF244. 1 hit.
PfamiPF02318. FYVE_2. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rabphilin and Noc2 are recruited to dense-core vesicles through specific interaction with Rab27A in PC12 cells."
    Fukuda M., Kanno E., Yamamoto A.
    J. Biol. Chem. 279:13065-13075(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, DOMAIN, MUTAGENESIS OF 51-GLU--ILE-55.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH RAB3A; RAB3B; RAB3C AND RAB3D.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2."
    Fukuda M.
    J. Biol. Chem. 278:15373-15380(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, INTERACTION WITH WITH RAB3A; RAB3B; RAB3C; RAB3D AND RAB27A.
  7. "Cellular expression of Noc2, a Rab effector protein, in endocrine and exocrine tissues in the mouse."
    Teramae H., Fujimoto W., Seino S., Iwanaga T.
    Histochem. Cell Biol. 127:1-11(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Pancreas.

Entry informationi

Entry nameiRPH3L_MOUSE
AccessioniPrimary (citable) accession number: Q768S4
Secondary accession number(s): Q9D353
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.