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Q766Z3

- REV3_ARATH

UniProt

Q766Z3 - REV3_ARATH

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Protein

DNA polymerase zeta catalytic subunit

Gene

REV3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the error prone DNA polymerase zeta. Involved in damage-tolerance mechanisms through translesion DNA synthesis.4 Publications

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Binds 1 4Fe-4S cluster.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1789 – 17891ZincBy similarity
Metal bindingi1792 – 17921ZincBy similarity
Metal bindingi1803 – 18031ZincBy similarity
Metal bindingi1806 – 18061ZincBy similarity
Metal bindingi1835 – 18351Iron-sulfur (4Fe-4S)By similarity
Metal bindingi1838 – 18381Iron-sulfur (4Fe-4S)By similarity
Metal bindingi1851 – 18511Iron-sulfur (4Fe-4S)By similarity
Metal bindingi1856 – 18561Iron-sulfur (4Fe-4S)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1789 – 180618CysA-typeBy similarityAdd
BLAST

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
  5. nucleotide binding Source: InterPro

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: UniProtKB
  2. cellular response to UV-C Source: UniProtKB
  3. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT1G67500-MONOMER.
ARA:GQT-271-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase zeta catalytic subunit (EC:2.7.7.7)
Alternative name(s):
Protein reversionless 3-like
Short name:
AtREV3
Gene namesi
Name:REV3
Ordered Locus Names:At1g67500
ORF Names:F12B7.5, T1F15.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G67500.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants are hypersensitive to UV-B light and gamma rays.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18901890DNA polymerase zeta catalytic subunitPRO_0000424418Add
BLAST

Proteomic databases

PRIDEiQ766Z3.

Expressioni

Tissue specificityi

Expressed in roots, leaves and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ766Z3. baseline and differential.
GenevestigatoriQ766Z3.

Interactioni

Subunit structurei

Forms DNA polymerase zeta with REV7.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G67500.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ766Z3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1835 – 185622CysB motifBy similarityAdd
BLAST

Domaini

The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.By similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated
Contains 1 CysA-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1789 – 180618CysA-typeBy similarityAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

KOiK02350.
PhylomeDBiQ766Z3.

Family and domain databases

Gene3Di3.30.420.10. 2 hits.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
IPR025687. Znf-C4pol.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14260. zf-C4pol. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 3 hits.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q766Z3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADSQSGSNV FSLRIVSIDY YMASPIPGYN ICYSSFQGSE VNEVPVIRIY
60 70 80 90 100
GSTPAGQKTC LHIHRALPYL YIPCSEIPLE HHKGVDGSTL ALSLELEKAL
110 120 130 140 150
KLKGNAASKR QHIHDCEIVR AKKFYGYHST EEAFVKIYLS YHPPDVARAA
160 170 180 190 200
SLLLAGAVLG KSLQPYESHI PFILQFLVDY NLYGMGHVHI SKMKFRSPVP
210 220 230 240 250
HHFRPRRFDL DDCPGQRIDE VAITKANSSA AASVSFPVWS LSTIPGQWMW
260 270 280 290 300
NLSEESDTPL SQSQHRHQHH YRRQSLCELE GDATSSDILN QQFKMYNSLS
310 320 330 340 350
QAQSDTNMVQ SLVAIWEEEY ERTGVHDAPI PPDPGKPSAA DVLQTMSDYV
360 370 380 390 400
GFGNMLKEML NKVELSPPGM KPTAVSSAGP DMHAKPEITD LQALNHMVGT
410 420 430 440 450
CSEFPASEQL SPLGEKSEEA SMENDEYMKT PTDRDTPAQI QDAEALGLFK
460 470 480 490 500
WFASSQAAED INSDDEILRE TILSPLLPLA SINKVLEMAS TDYVSQSQKE
510 520 530 540 550
CQDILDSQEN LPDFGSSTKR ALPSNPDSQN LRTSSDKQSL EIEVASDVPD
560 570 580 590 600
SSTSNGASEN SFRRYRKSDL HTSEVMEYKN RSFSKSNKPS NSVWGPLPFT
610 620 630 640 650
LTKNLQKDFD STNASDKLGL TKISSYPMNE MTDNYIVPVK EHQADVCNTI
660 670 680 690 700
DRNVLAGCSL RDLMRKKRLC HGESPVSQHM KSRKVRDSRH GEKNECTLRC
710 720 730 740 750
EAKKQGPALS AEFSEFVCGD TPNLSPIDSG NCECNISTES SELHSVDRCS
760 770 780 790 800
AKETASQNSD EVLRNLSSTT VPFGKDPQTV ESGTLVSSNI HVGIEIDSVQ
810 820 830 840 850
KSGREQESTA NETDETGRLI CLTLSKKPPS LDCLSAGLQD SAHSHEIHAR
860 870 880 890 900
EKQHDEYEGN SNDIPFFPLE DNKEEKKHFF QGTSLGIPLH HLNDGSNLYL
910 920 930 940 950
LTPAFSPPSV DSVLQWISND KGDSNIDSEK QPLRDNHNDR GASFTDLASA
960 970 980 990 1000
SNVVSVSEHV EQHNNLFVNS ESNAYTESEI DLKPKGTFLN LNLQASVSQE
1010 1020 1030 1040 1050
LSQISGPDGK SGPTPLSQMG FRDPASMGAG QQLTILSIEV HAESRGDLRP
1060 1070 1080 1090 1100
DPRFDSVNVI ALVVQNDDSF VAEVFVLLFS PDSIDQRNVD GLSGCKLSVF
1110 1120 1130 1140 1150
LEERQLFRYF IETLCKWDPD VLLGWDIQGG SIGFLAERAA QLGIRFLNNI
1160 1170 1180 1190 1200
SRTPSPTTTN NSDNKRKLGN NLLPDPLVAN PAQVEEVVIE DEWGRTHASG
1210 1220 1230 1240 1250
VHVGGRIVLN AWRLIRGEVK LNMYTIEAVS EAVLRQKVPS IPYKVLTEWF
1260 1270 1280 1290 1300
SSGPAGARYR CIEYVIRRAN LNLEIMSQLD MINRTSELAR VFGIDFFSVL
1310 1320 1330 1340 1350
SRGSQYRVES MLLRLAHTQN YLAISPGNQQ VASQPAMECV PLVMEPESAF
1360 1370 1380 1390 1400
YDDPVIVLDF QSLYPSMIIA YNLCFSTCLG KLAHLKMNTL GVSSYSLDLD
1410 1420 1430 1440 1450
VLQDLNQILQ TPNSVMYVPP EVRRGILPRL LEEILSTRIM VKKAMKKLTP
1460 1470 1480 1490 1500
SEAVLHRIFN ARQLALKLIA NVTYGYTAAG FSGRMPCAEL ADSIVQCGRS
1510 1520 1530 1540 1550
TLEKAISFVN ANDNWNARVV YGDTDSMFVL LKGRTVKEAF VVGQEIASAI
1560 1570 1580 1590 1600
TEMNPHPVTL KMEKVYHPCF LLTKKRYVGY SYESPNQREP IFDAKGIETV
1610 1620 1630 1640 1650
RRDTCEAVAK TMEQSLRLFF EQKNISKVKS YLYRQWKRIL SGRVSLQDFI
1660 1670 1680 1690 1700
FAKEVRLGTY STRDSSLLPP AAIVATKSMK ADPRTEPRYA ERVPYVVIHG
1710 1720 1730 1740 1750
EPGARLVDMV VDPLVLLDVD TPYRLNDLYY INKQIIPALQ RVFGLVGADL
1760 1770 1780 1790 1800
NQWFLEMPRL TRSSLGQRPL NSKNSHKTRI DYFYLSKHCI LCGEVVQESA
1810 1820 1830 1840 1850
QLCNRCLQNK SAAAATIVWK TSKLEREMQH LATICRHCGG GDWVVQSGVK
1860 1870 1880 1890
CNSLACSVFY ERRKVQKELR GLSSIATESE LYPKCMAEWF
Length:1,890
Mass (Da):210,848
Last modified:July 5, 2004 - v1
Checksum:i7E3E5BF3C140C105
GO

Sequence cautioni

The sequence AAC18785.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence AAG52299.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB114052 mRNA. Translation: BAC82450.1.
AC004393 Genomic DNA. Translation: AAC18785.1. Sequence problems.
AC011020 Genomic DNA. Translation: AAG52299.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34655.1.
PIRiD96698.
T02155.
RefSeqiNP_176917.2. NM_105417.3. [Q766Z3-1]
UniGeneiAt.35644.

Genome annotation databases

EnsemblPlantsiAT1G67500.1; AT1G67500.1; AT1G67500. [Q766Z3-1]
GeneIDi843071.
KEGGiath:AT1G67500.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB114052 mRNA. Translation: BAC82450.1 .
AC004393 Genomic DNA. Translation: AAC18785.1 . Sequence problems.
AC011020 Genomic DNA. Translation: AAG52299.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE34655.1 .
PIRi D96698.
T02155.
RefSeqi NP_176917.2. NM_105417.3. [Q766Z3-1 ]
UniGenei At.35644.

3D structure databases

ProteinModelPortali Q766Z3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G67500.1-P.

Proteomic databases

PRIDEi Q766Z3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G67500.1 ; AT1G67500.1 ; AT1G67500 . [Q766Z3-1 ]
GeneIDi 843071.
KEGGi ath:AT1G67500.

Organism-specific databases

TAIRi AT1G67500.

Phylogenomic databases

KOi K02350.
PhylomeDBi Q766Z3.

Enzyme and pathway databases

BioCyci ARA:AT1G67500-MONOMER.
ARA:GQT-271-MONOMER.

Miscellaneous databases

PROi Q766Z3.

Gene expression databases

ExpressionAtlasi Q766Z3. baseline and differential.
Genevestigatori Q766Z3.

Family and domain databases

Gene3Di 3.30.420.10. 2 hits.
3.90.1600.10. 2 hits.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
IPR025687. Znf-C4pol.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14260. zf-C4pol. 1 hit.
[Graphical view ]
PRINTSi PR00106. DNAPOLB.
SMARTi SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF53098. SSF53098. 3 hits.
PROSITEi PS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and gamma-rays in Arabidopsis: implication of the presence of a translesion synthesis mechanism in plants."
    Sakamoto A., Vo L.T., Hase Y., Shikazono N., Matsunaga T., Tanaka A.
    Plant Cell 15:2042-2057(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Roles of Arabidopsis AtREV1 and AtREV7 in translesion synthesis."
    Takahashi S., Sakamoto A., Sato S., Kato T., Tabata S., Tanaka A.
    Plant Physiol. 138:870-881(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Role of AtPolzeta, AtRev1, and AtPoleta in UV light-induced mutagenesis in Arabidopsis."
    Nakagawa M., Takahashi S., Tanaka A., Narumi I., Sakamoto A.N.
    Plant Physiol. 155:414-420(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "RAD5a and REV3 function in two alternative pathways of DNA-damage tolerance in Arabidopsis."
    Wang S., Wen R., Shi X., Lambrecht A., Wang H., Xiao W.
    DNA Repair 10:620-628(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiREV3_ARATH
AccessioniPrimary (citable) accession number: Q766Z3
Secondary accession number(s): O64795, Q9CAG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3