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Q766Z3

- REV3_ARATH

UniProt

Q766Z3 - REV3_ARATH

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Protein
DNA polymerase zeta catalytic subunit
Gene
REV3, At1g67500, F12B7.5, T1F15.3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the error prone DNA polymerase zeta. Involved in damage-tolerance mechanisms through translesion DNA synthesis.4 Publications

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Binds 1 4Fe-4S cluster.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1789 – 17891Zinc By similarity
Metal bindingi1792 – 17921Zinc By similarity
Metal bindingi1803 – 18031Zinc By similarity
Metal bindingi1806 – 18061Zinc By similarity
Metal bindingi1835 – 18351Iron-sulfur (4Fe-4S) By similarity
Metal bindingi1838 – 18381Iron-sulfur (4Fe-4S) By similarity
Metal bindingi1851 – 18511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi1856 – 18561Iron-sulfur (4Fe-4S) By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1789 – 180618CysA-type By similarity
Add
BLAST

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
  5. nucleotide binding Source: InterPro

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. cellular response to DNA damage stimulus Source: UniProtKB
  3. cellular response to UV-C Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT1G67500-MONOMER.
ARA:GQT-271-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase zeta catalytic subunit (EC:2.7.7.7)
Alternative name(s):
Protein reversionless 3-like
Short name:
AtREV3
Gene namesi
Name:REV3
Ordered Locus Names:At1g67500
ORF Names:F12B7.5, T1F15.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G67500.

Subcellular locationi

Nucleus Reviewed prediction

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants are hypersensitive to UV-B light and gamma rays.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18901890DNA polymerase zeta catalytic subunit
PRO_0000424418Add
BLAST

Proteomic databases

PRIDEiQ766Z3.

Expressioni

Tissue specificityi

Expressed in roots, leaves and flowers.1 Publication

Gene expression databases

ArrayExpressiQ766Z3.
GenevestigatoriQ766Z3.

Interactioni

Subunit structurei

Forms DNA polymerase zeta with REV7 By similarity.

Protein-protein interaction databases

STRINGi3702.AT1G67500.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ766Z3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1835 – 185622CysB motif By similarity
Add
BLAST

Domaini

The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes By similarity.

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiQ766Z3.
KOiK02350.
PhylomeDBiQ766Z3.

Family and domain databases

Gene3Di3.30.420.10. 2 hits.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
IPR025687. Znf-C4pol.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14260. zf-C4pol. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 3 hits.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q766Z3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MADSQSGSNV FSLRIVSIDY YMASPIPGYN ICYSSFQGSE VNEVPVIRIY     50
GSTPAGQKTC LHIHRALPYL YIPCSEIPLE HHKGVDGSTL ALSLELEKAL 100
KLKGNAASKR QHIHDCEIVR AKKFYGYHST EEAFVKIYLS YHPPDVARAA 150
SLLLAGAVLG KSLQPYESHI PFILQFLVDY NLYGMGHVHI SKMKFRSPVP 200
HHFRPRRFDL DDCPGQRIDE VAITKANSSA AASVSFPVWS LSTIPGQWMW 250
NLSEESDTPL SQSQHRHQHH YRRQSLCELE GDATSSDILN QQFKMYNSLS 300
QAQSDTNMVQ SLVAIWEEEY ERTGVHDAPI PPDPGKPSAA DVLQTMSDYV 350
GFGNMLKEML NKVELSPPGM KPTAVSSAGP DMHAKPEITD LQALNHMVGT 400
CSEFPASEQL SPLGEKSEEA SMENDEYMKT PTDRDTPAQI QDAEALGLFK 450
WFASSQAAED INSDDEILRE TILSPLLPLA SINKVLEMAS TDYVSQSQKE 500
CQDILDSQEN LPDFGSSTKR ALPSNPDSQN LRTSSDKQSL EIEVASDVPD 550
SSTSNGASEN SFRRYRKSDL HTSEVMEYKN RSFSKSNKPS NSVWGPLPFT 600
LTKNLQKDFD STNASDKLGL TKISSYPMNE MTDNYIVPVK EHQADVCNTI 650
DRNVLAGCSL RDLMRKKRLC HGESPVSQHM KSRKVRDSRH GEKNECTLRC 700
EAKKQGPALS AEFSEFVCGD TPNLSPIDSG NCECNISTES SELHSVDRCS 750
AKETASQNSD EVLRNLSSTT VPFGKDPQTV ESGTLVSSNI HVGIEIDSVQ 800
KSGREQESTA NETDETGRLI CLTLSKKPPS LDCLSAGLQD SAHSHEIHAR 850
EKQHDEYEGN SNDIPFFPLE DNKEEKKHFF QGTSLGIPLH HLNDGSNLYL 900
LTPAFSPPSV DSVLQWISND KGDSNIDSEK QPLRDNHNDR GASFTDLASA 950
SNVVSVSEHV EQHNNLFVNS ESNAYTESEI DLKPKGTFLN LNLQASVSQE 1000
LSQISGPDGK SGPTPLSQMG FRDPASMGAG QQLTILSIEV HAESRGDLRP 1050
DPRFDSVNVI ALVVQNDDSF VAEVFVLLFS PDSIDQRNVD GLSGCKLSVF 1100
LEERQLFRYF IETLCKWDPD VLLGWDIQGG SIGFLAERAA QLGIRFLNNI 1150
SRTPSPTTTN NSDNKRKLGN NLLPDPLVAN PAQVEEVVIE DEWGRTHASG 1200
VHVGGRIVLN AWRLIRGEVK LNMYTIEAVS EAVLRQKVPS IPYKVLTEWF 1250
SSGPAGARYR CIEYVIRRAN LNLEIMSQLD MINRTSELAR VFGIDFFSVL 1300
SRGSQYRVES MLLRLAHTQN YLAISPGNQQ VASQPAMECV PLVMEPESAF 1350
YDDPVIVLDF QSLYPSMIIA YNLCFSTCLG KLAHLKMNTL GVSSYSLDLD 1400
VLQDLNQILQ TPNSVMYVPP EVRRGILPRL LEEILSTRIM VKKAMKKLTP 1450
SEAVLHRIFN ARQLALKLIA NVTYGYTAAG FSGRMPCAEL ADSIVQCGRS 1500
TLEKAISFVN ANDNWNARVV YGDTDSMFVL LKGRTVKEAF VVGQEIASAI 1550
TEMNPHPVTL KMEKVYHPCF LLTKKRYVGY SYESPNQREP IFDAKGIETV 1600
RRDTCEAVAK TMEQSLRLFF EQKNISKVKS YLYRQWKRIL SGRVSLQDFI 1650
FAKEVRLGTY STRDSSLLPP AAIVATKSMK ADPRTEPRYA ERVPYVVIHG 1700
EPGARLVDMV VDPLVLLDVD TPYRLNDLYY INKQIIPALQ RVFGLVGADL 1750
NQWFLEMPRL TRSSLGQRPL NSKNSHKTRI DYFYLSKHCI LCGEVVQESA 1800
QLCNRCLQNK SAAAATIVWK TSKLEREMQH LATICRHCGG GDWVVQSGVK 1850
CNSLACSVFY ERRKVQKELR GLSSIATESE LYPKCMAEWF 1890
Length:1,890
Mass (Da):210,848
Last modified:July 5, 2004 - v1
Checksum:i7E3E5BF3C140C105
GO

Sequence cautioni

The sequence AAC18785.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence AAG52299.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB114052 mRNA. Translation: BAC82450.1.
AC004393 Genomic DNA. Translation: AAC18785.1. Sequence problems.
AC011020 Genomic DNA. Translation: AAG52299.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34655.1.
PIRiD96698.
T02155.
RefSeqiNP_176917.2. NM_105417.3. [Q766Z3-1]
UniGeneiAt.35644.

Genome annotation databases

EnsemblPlantsiAT1G67500.1; AT1G67500.1; AT1G67500. [Q766Z3-1]
GeneIDi843071.
KEGGiath:AT1G67500.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB114052 mRNA. Translation: BAC82450.1 .
AC004393 Genomic DNA. Translation: AAC18785.1 . Sequence problems.
AC011020 Genomic DNA. Translation: AAG52299.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE34655.1 .
PIRi D96698.
T02155.
RefSeqi NP_176917.2. NM_105417.3. [Q766Z3-1 ]
UniGenei At.35644.

3D structure databases

ProteinModelPortali Q766Z3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G67500.1-P.

Proteomic databases

PRIDEi Q766Z3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G67500.1 ; AT1G67500.1 ; AT1G67500 . [Q766Z3-1 ]
GeneIDi 843071.
KEGGi ath:AT1G67500.

Organism-specific databases

TAIRi AT1G67500.

Phylogenomic databases

InParanoidi Q766Z3.
KOi K02350.
PhylomeDBi Q766Z3.

Enzyme and pathway databases

BioCyci ARA:AT1G67500-MONOMER.
ARA:GQT-271-MONOMER.

Miscellaneous databases

PROi Q766Z3.

Gene expression databases

ArrayExpressi Q766Z3.
Genevestigatori Q766Z3.

Family and domain databases

Gene3Di 3.30.420.10. 2 hits.
3.90.1600.10. 2 hits.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
IPR025687. Znf-C4pol.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14260. zf-C4pol. 1 hit.
[Graphical view ]
PRINTSi PR00106. DNAPOLB.
SMARTi SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF53098. SSF53098. 3 hits.
PROSITEi PS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and gamma-rays in Arabidopsis: implication of the presence of a translesion synthesis mechanism in plants."
    Sakamoto A., Vo L.T., Hase Y., Shikazono N., Matsunaga T., Tanaka A.
    Plant Cell 15:2042-2057(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Roles of Arabidopsis AtREV1 and AtREV7 in translesion synthesis."
    Takahashi S., Sakamoto A., Sato S., Kato T., Tabata S., Tanaka A.
    Plant Physiol. 138:870-881(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Role of AtPolzeta, AtRev1, and AtPoleta in UV light-induced mutagenesis in Arabidopsis."
    Nakagawa M., Takahashi S., Tanaka A., Narumi I., Sakamoto A.N.
    Plant Physiol. 155:414-420(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "RAD5a and REV3 function in two alternative pathways of DNA-damage tolerance in Arabidopsis."
    Wang S., Wen R., Shi X., Lambrecht A., Wang H., Xiao W.
    DNA Repair 10:620-628(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiREV3_ARATH
AccessioniPrimary (citable) accession number: Q766Z3
Secondary accession number(s): O64795, Q9CAG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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