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Q766Z3

- REV3_ARATH

UniProt

Q766Z3 - REV3_ARATH

Protein

DNA polymerase zeta catalytic subunit

Gene

REV3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the error prone DNA polymerase zeta. Involved in damage-tolerance mechanisms through translesion DNA synthesis.4 Publications

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    Cofactori

    Binds 1 4Fe-4S cluster.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi1789 – 17891ZincBy similarity
    Metal bindingi1792 – 17921ZincBy similarity
    Metal bindingi1803 – 18031ZincBy similarity
    Metal bindingi1806 – 18061ZincBy similarity
    Metal bindingi1835 – 18351Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi1838 – 18381Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi1851 – 18511Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi1856 – 18561Iron-sulfur (4Fe-4S)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1789 – 180618CysA-typeBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. nucleotide binding Source: InterPro

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: UniProtKB
    2. cellular response to UV-C Source: UniProtKB
    3. DNA repair Source: UniProtKB-KW

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA damage, DNA repair, DNA replication

    Keywords - Ligandi

    4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT1G67500-MONOMER.
    ARA:GQT-271-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase zeta catalytic subunit (EC:2.7.7.7)
    Alternative name(s):
    Protein reversionless 3-like
    Short name:
    AtREV3
    Gene namesi
    Name:REV3
    Ordered Locus Names:At1g67500
    ORF Names:F12B7.5, T1F15.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G67500.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but mutant plants are hypersensitive to UV-B light and gamma rays.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 18901890DNA polymerase zeta catalytic subunitPRO_0000424418Add
    BLAST

    Proteomic databases

    PRIDEiQ766Z3.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves and flowers.1 Publication

    Gene expression databases

    ArrayExpressiQ766Z3.
    GenevestigatoriQ766Z3.

    Interactioni

    Subunit structurei

    Forms DNA polymerase zeta with REV7.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT1G67500.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ766Z3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1835 – 185622CysB motifBy similarityAdd
    BLAST

    Domaini

    The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.By similarity

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B family.Curated
    Contains 1 CysA-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1789 – 180618CysA-typeBy similarityAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    InParanoidiQ766Z3.
    KOiK02350.
    PhylomeDBiQ766Z3.

    Family and domain databases

    Gene3Di3.30.420.10. 2 hits.
    3.90.1600.10. 2 hits.
    InterProiIPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR012337. RNaseH-like_dom.
    IPR025687. Znf-C4pol.
    [Graphical view]
    PfamiPF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 2 hits.
    PF14260. zf-C4pol. 1 hit.
    [Graphical view]
    PRINTSiPR00106. DNAPOLB.
    SMARTiSM00486. POLBc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 3 hits.
    PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q766Z3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADSQSGSNV FSLRIVSIDY YMASPIPGYN ICYSSFQGSE VNEVPVIRIY     50
    GSTPAGQKTC LHIHRALPYL YIPCSEIPLE HHKGVDGSTL ALSLELEKAL 100
    KLKGNAASKR QHIHDCEIVR AKKFYGYHST EEAFVKIYLS YHPPDVARAA 150
    SLLLAGAVLG KSLQPYESHI PFILQFLVDY NLYGMGHVHI SKMKFRSPVP 200
    HHFRPRRFDL DDCPGQRIDE VAITKANSSA AASVSFPVWS LSTIPGQWMW 250
    NLSEESDTPL SQSQHRHQHH YRRQSLCELE GDATSSDILN QQFKMYNSLS 300
    QAQSDTNMVQ SLVAIWEEEY ERTGVHDAPI PPDPGKPSAA DVLQTMSDYV 350
    GFGNMLKEML NKVELSPPGM KPTAVSSAGP DMHAKPEITD LQALNHMVGT 400
    CSEFPASEQL SPLGEKSEEA SMENDEYMKT PTDRDTPAQI QDAEALGLFK 450
    WFASSQAAED INSDDEILRE TILSPLLPLA SINKVLEMAS TDYVSQSQKE 500
    CQDILDSQEN LPDFGSSTKR ALPSNPDSQN LRTSSDKQSL EIEVASDVPD 550
    SSTSNGASEN SFRRYRKSDL HTSEVMEYKN RSFSKSNKPS NSVWGPLPFT 600
    LTKNLQKDFD STNASDKLGL TKISSYPMNE MTDNYIVPVK EHQADVCNTI 650
    DRNVLAGCSL RDLMRKKRLC HGESPVSQHM KSRKVRDSRH GEKNECTLRC 700
    EAKKQGPALS AEFSEFVCGD TPNLSPIDSG NCECNISTES SELHSVDRCS 750
    AKETASQNSD EVLRNLSSTT VPFGKDPQTV ESGTLVSSNI HVGIEIDSVQ 800
    KSGREQESTA NETDETGRLI CLTLSKKPPS LDCLSAGLQD SAHSHEIHAR 850
    EKQHDEYEGN SNDIPFFPLE DNKEEKKHFF QGTSLGIPLH HLNDGSNLYL 900
    LTPAFSPPSV DSVLQWISND KGDSNIDSEK QPLRDNHNDR GASFTDLASA 950
    SNVVSVSEHV EQHNNLFVNS ESNAYTESEI DLKPKGTFLN LNLQASVSQE 1000
    LSQISGPDGK SGPTPLSQMG FRDPASMGAG QQLTILSIEV HAESRGDLRP 1050
    DPRFDSVNVI ALVVQNDDSF VAEVFVLLFS PDSIDQRNVD GLSGCKLSVF 1100
    LEERQLFRYF IETLCKWDPD VLLGWDIQGG SIGFLAERAA QLGIRFLNNI 1150
    SRTPSPTTTN NSDNKRKLGN NLLPDPLVAN PAQVEEVVIE DEWGRTHASG 1200
    VHVGGRIVLN AWRLIRGEVK LNMYTIEAVS EAVLRQKVPS IPYKVLTEWF 1250
    SSGPAGARYR CIEYVIRRAN LNLEIMSQLD MINRTSELAR VFGIDFFSVL 1300
    SRGSQYRVES MLLRLAHTQN YLAISPGNQQ VASQPAMECV PLVMEPESAF 1350
    YDDPVIVLDF QSLYPSMIIA YNLCFSTCLG KLAHLKMNTL GVSSYSLDLD 1400
    VLQDLNQILQ TPNSVMYVPP EVRRGILPRL LEEILSTRIM VKKAMKKLTP 1450
    SEAVLHRIFN ARQLALKLIA NVTYGYTAAG FSGRMPCAEL ADSIVQCGRS 1500
    TLEKAISFVN ANDNWNARVV YGDTDSMFVL LKGRTVKEAF VVGQEIASAI 1550
    TEMNPHPVTL KMEKVYHPCF LLTKKRYVGY SYESPNQREP IFDAKGIETV 1600
    RRDTCEAVAK TMEQSLRLFF EQKNISKVKS YLYRQWKRIL SGRVSLQDFI 1650
    FAKEVRLGTY STRDSSLLPP AAIVATKSMK ADPRTEPRYA ERVPYVVIHG 1700
    EPGARLVDMV VDPLVLLDVD TPYRLNDLYY INKQIIPALQ RVFGLVGADL 1750
    NQWFLEMPRL TRSSLGQRPL NSKNSHKTRI DYFYLSKHCI LCGEVVQESA 1800
    QLCNRCLQNK SAAAATIVWK TSKLEREMQH LATICRHCGG GDWVVQSGVK 1850
    CNSLACSVFY ERRKVQKELR GLSSIATESE LYPKCMAEWF 1890
    Length:1,890
    Mass (Da):210,848
    Last modified:July 5, 2004 - v1
    Checksum:i7E3E5BF3C140C105
    GO

    Sequence cautioni

    The sequence AAC18785.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence AAG52299.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB114052 mRNA. Translation: BAC82450.1.
    AC004393 Genomic DNA. Translation: AAC18785.1. Sequence problems.
    AC011020 Genomic DNA. Translation: AAG52299.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34655.1.
    PIRiD96698.
    T02155.
    RefSeqiNP_176917.2. NM_105417.3. [Q766Z3-1]
    UniGeneiAt.35644.

    Genome annotation databases

    EnsemblPlantsiAT1G67500.1; AT1G67500.1; AT1G67500. [Q766Z3-1]
    GeneIDi843071.
    KEGGiath:AT1G67500.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB114052 mRNA. Translation: BAC82450.1 .
    AC004393 Genomic DNA. Translation: AAC18785.1 . Sequence problems.
    AC011020 Genomic DNA. Translation: AAG52299.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE34655.1 .
    PIRi D96698.
    T02155.
    RefSeqi NP_176917.2. NM_105417.3. [Q766Z3-1 ]
    UniGenei At.35644.

    3D structure databases

    ProteinModelPortali Q766Z3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G67500.1-P.

    Proteomic databases

    PRIDEi Q766Z3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G67500.1 ; AT1G67500.1 ; AT1G67500 . [Q766Z3-1 ]
    GeneIDi 843071.
    KEGGi ath:AT1G67500.

    Organism-specific databases

    TAIRi AT1G67500.

    Phylogenomic databases

    InParanoidi Q766Z3.
    KOi K02350.
    PhylomeDBi Q766Z3.

    Enzyme and pathway databases

    BioCyci ARA:AT1G67500-MONOMER.
    ARA:GQT-271-MONOMER.

    Miscellaneous databases

    PROi Q766Z3.

    Gene expression databases

    ArrayExpressi Q766Z3.
    Genevestigatori Q766Z3.

    Family and domain databases

    Gene3Di 3.30.420.10. 2 hits.
    3.90.1600.10. 2 hits.
    InterProi IPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR012337. RNaseH-like_dom.
    IPR025687. Znf-C4pol.
    [Graphical view ]
    Pfami PF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 2 hits.
    PF14260. zf-C4pol. 1 hit.
    [Graphical view ]
    PRINTSi PR00106. DNAPOLB.
    SMARTi SM00486. POLBc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 3 hits.
    PROSITEi PS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and gamma-rays in Arabidopsis: implication of the presence of a translesion synthesis mechanism in plants."
      Sakamoto A., Vo L.T., Hase Y., Shikazono N., Matsunaga T., Tanaka A.
      Plant Cell 15:2042-2057(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Roles of Arabidopsis AtREV1 and AtREV7 in translesion synthesis."
      Takahashi S., Sakamoto A., Sato S., Kato T., Tabata S., Tanaka A.
      Plant Physiol. 138:870-881(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Role of AtPolzeta, AtRev1, and AtPoleta in UV light-induced mutagenesis in Arabidopsis."
      Nakagawa M., Takahashi S., Tanaka A., Narumi I., Sakamoto A.N.
      Plant Physiol. 155:414-420(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "RAD5a and REV3 function in two alternative pathways of DNA-damage tolerance in Arabidopsis."
      Wang S., Wen R., Shi X., Lambrecht A., Wang H., Xiao W.
      DNA Repair 10:620-628(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiREV3_ARATH
    AccessioniPrimary (citable) accession number: Q766Z3
    Secondary accession number(s): O64795, Q9CAG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2013
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3