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Q766Z3 (REV3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase zeta catalytic subunit

EC=2.7.7.7
Alternative name(s):
Protein reversionless 3-like
Short name=AtREV3
Gene names
Name:REV3
Ordered Locus Names:At1g67500
ORF Names:F12B7.5, T1F15.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1890 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalytic subunit of the error prone DNA polymerase zeta. Involved in damage-tolerance mechanisms through translesion DNA synthesis. Ref.1 Ref.4 Ref.5 Ref.6

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactor

Binds 1 4Fe-4S cluster.

Subunit structure

Forms DNA polymerase zeta with REV7 By similarity.

Subcellular location

Nucleus Potential.

Tissue specificity

Expressed in roots, leaves and flowers. Ref.1

Domain

The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes By similarity.

Disruption phenotype

No visible phenotype under normal growth conditions, but mutant plants are hypersensitive to UV-B light and gamma rays. Ref.1

Sequence similarities

Belongs to the DNA polymerase type-B family.

Contains 1 CysA-type zinc finger.

Sequence caution

The sequence AAC18785.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAG52299.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q766Z3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18901890DNA polymerase zeta catalytic subunit
PRO_0000424418

Regions

Zinc finger1789 – 180618CysA-type By similarity
Motif1835 – 185622CysB motif By similarity

Sites

Metal binding17891Zinc By similarity
Metal binding17921Zinc By similarity
Metal binding18031Zinc By similarity
Metal binding18061Zinc By similarity
Metal binding18351Iron-sulfur (4Fe-4S) By similarity
Metal binding18381Iron-sulfur (4Fe-4S) By similarity
Metal binding18511Iron-sulfur (4Fe-4S) By similarity
Metal binding18561Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 7E3E5BF3C140C105

FASTA1,890210,848
        10         20         30         40         50         60 
MADSQSGSNV FSLRIVSIDY YMASPIPGYN ICYSSFQGSE VNEVPVIRIY GSTPAGQKTC 

        70         80         90        100        110        120 
LHIHRALPYL YIPCSEIPLE HHKGVDGSTL ALSLELEKAL KLKGNAASKR QHIHDCEIVR 

       130        140        150        160        170        180 
AKKFYGYHST EEAFVKIYLS YHPPDVARAA SLLLAGAVLG KSLQPYESHI PFILQFLVDY 

       190        200        210        220        230        240 
NLYGMGHVHI SKMKFRSPVP HHFRPRRFDL DDCPGQRIDE VAITKANSSA AASVSFPVWS 

       250        260        270        280        290        300 
LSTIPGQWMW NLSEESDTPL SQSQHRHQHH YRRQSLCELE GDATSSDILN QQFKMYNSLS 

       310        320        330        340        350        360 
QAQSDTNMVQ SLVAIWEEEY ERTGVHDAPI PPDPGKPSAA DVLQTMSDYV GFGNMLKEML 

       370        380        390        400        410        420 
NKVELSPPGM KPTAVSSAGP DMHAKPEITD LQALNHMVGT CSEFPASEQL SPLGEKSEEA 

       430        440        450        460        470        480 
SMENDEYMKT PTDRDTPAQI QDAEALGLFK WFASSQAAED INSDDEILRE TILSPLLPLA 

       490        500        510        520        530        540 
SINKVLEMAS TDYVSQSQKE CQDILDSQEN LPDFGSSTKR ALPSNPDSQN LRTSSDKQSL 

       550        560        570        580        590        600 
EIEVASDVPD SSTSNGASEN SFRRYRKSDL HTSEVMEYKN RSFSKSNKPS NSVWGPLPFT 

       610        620        630        640        650        660 
LTKNLQKDFD STNASDKLGL TKISSYPMNE MTDNYIVPVK EHQADVCNTI DRNVLAGCSL 

       670        680        690        700        710        720 
RDLMRKKRLC HGESPVSQHM KSRKVRDSRH GEKNECTLRC EAKKQGPALS AEFSEFVCGD 

       730        740        750        760        770        780 
TPNLSPIDSG NCECNISTES SELHSVDRCS AKETASQNSD EVLRNLSSTT VPFGKDPQTV 

       790        800        810        820        830        840 
ESGTLVSSNI HVGIEIDSVQ KSGREQESTA NETDETGRLI CLTLSKKPPS LDCLSAGLQD 

       850        860        870        880        890        900 
SAHSHEIHAR EKQHDEYEGN SNDIPFFPLE DNKEEKKHFF QGTSLGIPLH HLNDGSNLYL 

       910        920        930        940        950        960 
LTPAFSPPSV DSVLQWISND KGDSNIDSEK QPLRDNHNDR GASFTDLASA SNVVSVSEHV 

       970        980        990       1000       1010       1020 
EQHNNLFVNS ESNAYTESEI DLKPKGTFLN LNLQASVSQE LSQISGPDGK SGPTPLSQMG 

      1030       1040       1050       1060       1070       1080 
FRDPASMGAG QQLTILSIEV HAESRGDLRP DPRFDSVNVI ALVVQNDDSF VAEVFVLLFS 

      1090       1100       1110       1120       1130       1140 
PDSIDQRNVD GLSGCKLSVF LEERQLFRYF IETLCKWDPD VLLGWDIQGG SIGFLAERAA 

      1150       1160       1170       1180       1190       1200 
QLGIRFLNNI SRTPSPTTTN NSDNKRKLGN NLLPDPLVAN PAQVEEVVIE DEWGRTHASG 

      1210       1220       1230       1240       1250       1260 
VHVGGRIVLN AWRLIRGEVK LNMYTIEAVS EAVLRQKVPS IPYKVLTEWF SSGPAGARYR 

      1270       1280       1290       1300       1310       1320 
CIEYVIRRAN LNLEIMSQLD MINRTSELAR VFGIDFFSVL SRGSQYRVES MLLRLAHTQN 

      1330       1340       1350       1360       1370       1380 
YLAISPGNQQ VASQPAMECV PLVMEPESAF YDDPVIVLDF QSLYPSMIIA YNLCFSTCLG 

      1390       1400       1410       1420       1430       1440 
KLAHLKMNTL GVSSYSLDLD VLQDLNQILQ TPNSVMYVPP EVRRGILPRL LEEILSTRIM 

      1450       1460       1470       1480       1490       1500 
VKKAMKKLTP SEAVLHRIFN ARQLALKLIA NVTYGYTAAG FSGRMPCAEL ADSIVQCGRS 

      1510       1520       1530       1540       1550       1560 
TLEKAISFVN ANDNWNARVV YGDTDSMFVL LKGRTVKEAF VVGQEIASAI TEMNPHPVTL 

      1570       1580       1590       1600       1610       1620 
KMEKVYHPCF LLTKKRYVGY SYESPNQREP IFDAKGIETV RRDTCEAVAK TMEQSLRLFF 

      1630       1640       1650       1660       1670       1680 
EQKNISKVKS YLYRQWKRIL SGRVSLQDFI FAKEVRLGTY STRDSSLLPP AAIVATKSMK 

      1690       1700       1710       1720       1730       1740 
ADPRTEPRYA ERVPYVVIHG EPGARLVDMV VDPLVLLDVD TPYRLNDLYY INKQIIPALQ 

      1750       1760       1770       1780       1790       1800 
RVFGLVGADL NQWFLEMPRL TRSSLGQRPL NSKNSHKTRI DYFYLSKHCI LCGEVVQESA 

      1810       1820       1830       1840       1850       1860 
QLCNRCLQNK SAAAATIVWK TSKLEREMQH LATICRHCGG GDWVVQSGVK CNSLACSVFY 

      1870       1880       1890 
ERRKVQKELR GLSSIATESE LYPKCMAEWF 

« Hide

References

« Hide 'large scale' references
[1]"Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and gamma-rays in Arabidopsis: implication of the presence of a translesion synthesis mechanism in plants."
Sakamoto A., Vo L.T., Hase Y., Shikazono N., Matsunaga T., Tanaka A.
Plant Cell 15:2042-2057(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Roles of Arabidopsis AtREV1 and AtREV7 in translesion synthesis."
Takahashi S., Sakamoto A., Sato S., Kato T., Tabata S., Tanaka A.
Plant Physiol. 138:870-881(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Role of AtPolzeta, AtRev1, and AtPoleta in UV light-induced mutagenesis in Arabidopsis."
Nakagawa M., Takahashi S., Tanaka A., Narumi I., Sakamoto A.N.
Plant Physiol. 155:414-420(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"RAD5a and REV3 function in two alternative pathways of DNA-damage tolerance in Arabidopsis."
Wang S., Wen R., Shi X., Lambrecht A., Wang H., Xiao W.
DNA Repair 10:620-628(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB114052 mRNA. Translation: BAC82450.1.
AC004393 Genomic DNA. Translation: AAC18785.1. Sequence problems.
AC011020 Genomic DNA. Translation: AAG52299.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34655.1.
PIRD96698.
T02155.
RefSeqNP_176917.2. NM_105417.3. [Q766Z3-1]
UniGeneAt.35644.

3D structure databases

ProteinModelPortalQ766Z3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G67500.1-P.

Proteomic databases

PRIDEQ766Z3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G67500.1; AT1G67500.1; AT1G67500. [Q766Z3-1]
GeneID843071.
KEGGath:AT1G67500.

Organism-specific databases

TAIRAT1G67500.

Phylogenomic databases

InParanoidQ766Z3.
KOK02350.
PhylomeDBQ766Z3.

Enzyme and pathway databases

BioCycARA:AT1G67500-MONOMER.
ARA:GQT-271-MONOMER.

Gene expression databases

ArrayExpressQ766Z3.
GenevestigatorQ766Z3.

Family and domain databases

Gene3D3.30.420.10. 2 hits.
3.90.1600.10. 2 hits.
InterProIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
IPR025687. Znf-C4pol.
[Graphical view]
PfamPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14260. zf-C4pol. 1 hit.
[Graphical view]
PRINTSPR00106. DNAPOLB.
SMARTSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 3 hits.
PROSITEPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ766Z3.

Entry information

Entry nameREV3_ARATH
AccessionPrimary (citable) accession number: Q766Z3
Secondary accession number(s): O64795, Q9CAG6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names