Q766D5 (B4GN4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1
Short name=NGalNAc-T1
EC=2.4.1.244

Alternative name(s):
Beta-1,4-N-acetylgalactosaminyltransferase IV
Short name=Beta4GalNAc-T4
Short name=Beta4GalNAcT4

Gene names

Name:

B4galnt4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1034 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transfers N-acetylgalactosamine (GalNAc) from UDP-GalNAc to N-acetylglucosamine-beta-benzyl with a beta-1,4-linkage to form N,N'-diacetyllactosediamine, GalNAc-beta-1,4-GlcNAc structures in N-linked glycans and probably O-linked glycans By similarity.
Catalytic activity	UDP-N-acetyl-D-galactosamine + N-acetyl-beta-D-glucosaminyl group = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl group.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the chondroitin N-acetylgalactosaminyltransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q766D5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q766D5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-817: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1034	1034	N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1		PRO_0000252371
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 31	19	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	32 – 1034	1003	Lumenal Potential
	Compositional bias	474 – 566	93	Pro-rich
Amino acid modifications
	Glycosylation	106	1	N-linked (GlcNAc...) Potential
	Glycosylation	611	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 817	817	Missing in isoform 2.		VSP_020917

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2EF36F8710CA5B73	FASTA	1,034	117,373
	10 20 30 40 50 60 MPWFPVKKVR KQMKLLLLLL LLTCAAWLTY VHRSLVRPGR ALRQRLGYGR DGEKLTGVTD 70 80 90 100 110 120 SRGVRVPSST QRSEDSSESH EEEQAPEGRG PNMLFPGGPR KPPPLNLTHQ TPPWREEFKG 130 140 150 160 170 180 QVNLHVFEDW CGGAVGHLRR NLHFPLFPHT RTTVTKLAVS PKWKNYGLRI FGFIHPARDG 190 200 210 220 230 240 DIQFSVASDD NSEFWLSLDE SPAAAQLVAF VGKTGSEWTA PGEFTKFSSQ VSKPRRLMAS 250 260 270 280 290 300 RRYYFELLHK QDDKGSDHVE VGWRAFLPGL KFEIIDSAHI SLYTDESSLK MDHVAHVPQS 310 320 330 340 350 360 PASHIGGFPP QGEPSADMLH PDPRDTFFLT PRMEPLSLEN VLEPCAYAPT YILKDFPIAR 370 380 390 400 410 420 YQGLQFVYLS FIYPNDHTRL THMETDNKCF YRESPLYLER FGFYKYMKMD KEEGEEDEEE 430 440 450 460 470 480 EVQRRAFLFL NPDDFLDEED EQDLLDSLEP TDASVQQSHR TPTPAASTGT TASPTPPTTS 490 500 510 520 530 540 PLDEQTLRHS RALNWAPRPL PLFLGRAPPP RTVEKSPSKV YVTRVRPGQR ASPRALRDSP 550 560 570 580 590 600 WPPFPGVFLR PKPLPRVQLR VPPHPPRTQG YRTSGPKVTE LKPPVRAQTS QGGREGQLHG 610 620 630 640 650 660 QGLMVPTVDL NSSVETQPVT SFLSLSQVSR PQLPGEGEEG EEDGAPGDEA TSEDSEEEEE 670 680 690 700 710 720 PAAGRPLGRW REDAINWQRT FSVGAMDFEL LRSDWNDLRC NVSGNLQLPE AEAVDVVAQY 730 740 750 760 770 780 MERLNAKHGG RFSLLRIVNV EKRRDSARGS RFLLELELQE RGGSRQRLSE YVFLRLPGAR 790 800 810 820 830 840 VGDEDGESPE PPPAASIHPD SRPELCRPLH LAWRQDVMVH FIVPVKNQAR WVVQFLADMT 850 860 870 880 890 900 ALHVHTGDSY FNIILVDFES EDMDVERALR AAQLPRYQYL KRTGNFERSA GLQTGVDAVE 910 920 930 940 950 960 DPSSIVFLCD LHIHFPPNIL DSIRKHCVEG KLAFAPVVMR LGCGSSPWDP HGYWEVNGFG 970 980 990 1000 1010 1020 LFGIYKSDFD RVGGMNTEEF RDQWGGEDWE LLDRVLQAGL EVERLRLRHF YHHYHSKRGM 1030 WATRSRKGAR AQRS
	Isoform 2 [UniParc]. Checksum: 4BAFDA45368D8103	FASTA	217	25,065
	10 20 30 40 50 60 MVHFIVPVKN QARWVVQFLA DMTALHVHTG DSYFNIILVD FESEDMDVER ALRAAQLPRY 70 80 90 100 110 120 QYLKRTGNFE RSAGLQTGVD AVEDPSSIVF LCDLHIHFPP NILDSIRKHC VEGKLAFAPV 130 140 150 160 170 180 VMRLGCGSSP WDPHGYWEVN GFGLFGIYKS DFDRVGGMNT EEFRDQWGGE DWELLDRVLQ 190 200 210 AGLEVERLRL RHFYHHYHSK RGMWATRSRK GARAQRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N'-diacetyllactosediamine." Gotoh M., Sato T., Kiyohara K., Kameyama A., Kikuchi N., Kwon Y.-D., Ishizuka Y., Iwai T., Nakanishi H., Narimatsu H. FEBS Lett. 562:134-140(2004) [PubMed: 15044014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Mammary gland.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Beta1,4-N-acetylgalactosaminyltransferase IV.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB114827 mRNA. Translation: BAD15101.1. BC038881 mRNA. Translation: AAH38881.1.
IPI	IPI00319691. IPI00798479.
RefSeq	NP_808565.2. NM_177897.3.
UniGene	Mm.133741.
3D structure databases
ProteinModelPortal	Q766D5.
ModBase	Search...
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
PTM databases
PhosphoSite	Q766D5.
Proteomic databases
PRIDE	Q766D5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000048002; ENSMUSP00000039758; ENSMUSG00000055629.
GeneID	330671.
KEGG	mmu:330671.
UCSC	uc009kje.1. mouse. uc009kjg.1. mouse.
Organism-specific databases
CTD	338707.
MGI	MGI:2652891. B4galnt4.
Phylogenomic databases
eggNOG	roNOG13299.
GeneTree	ENSGT00530000063776.
HOGENOM	HBG446821.
HOVERGEN	HBG080886.
InParanoid	Q766D5.
OMA	LPGLKFE.
OrthoDB	EOG42RD6V.
PhylomeDB	Q766D5.
Gene expression databases
ArrayExpress	Q766D5.
Bgee	Q766D5.
CleanEx	MM_B4GALNT4.
Genevestigator	Q766D5.
GermOnline	ENSMUSG00000055629. Mus musculus.
Family and domain databases
InterPro	IPR008428. Chond_GalNAc. IPR011658. PA14. [Graphical view]
KO	K09657.
PANTHER	PTHR12369. CHGN. 1 hit.
Pfam	PF05679. CHGN. 1 hit. PF07691. PA14. 1 hit. [Graphical view]
SMART	SM00758. PA14. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	399502.
SOURCE	Search...

Entry information

Entry name

B4GN4_MOUSE

Accession

Primary (citable) accession number: Q766D5
Secondary accession number(s): Q8CHV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	July 5, 2004
Last modified:	November 16, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program