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Protein

N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1

Gene

B4galnt4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Transfers N-acetylgalactosamine (GalNAc) from UDP-GalNAc to N-acetylglucosamine-beta-benzyl with a beta-1,4-linkage to form N,N'-diacetyllactosediamine, GalNAc-beta-1,4-GlcNAc structures in N-linked glycans and probably O-linked glycans.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-beta-D-glucosaminyl group = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl group.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1 (EC:2.4.1.244)
Short name:
NGalNAc-T1
Alternative name(s):
Beta-1,4-N-acetylgalactosaminyltransferase IV
Short name:
Beta4GalNAc-T4
Short name:
Beta4GalNAcT4
Gene namesi
Name:B4galnt4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2652891. B4galnt4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3119Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini32 – 10341003LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10341034N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 1PRO_0000252371Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence analysis
Glycosylationi611 – 6111N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ766D5.
MaxQBiQ766D5.
PaxDbiQ766D5.
PeptideAtlasiQ766D5.
PRIDEiQ766D5.

PTM databases

PhosphoSiteiQ766D5.

Expressioni

Gene expression databases

BgeeiQ766D5.
CleanExiMM_B4GALNT4.
GenevisibleiQ766D5. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000039758.

Structurei

3D structure databases

ProteinModelPortaliQ766D5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi474 – 56693Pro-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3588. Eukaryota.
ENOG410XNYM. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000071102.
HOVERGENiHBG080886.
InParanoidiQ766D5.
KOiK09657.
OMAiGMWATRS.
OrthoDBiEOG7CG6ZB.
PhylomeDBiQ766D5.
TreeFamiTF318303.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
IPR011658. PA14_dom.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q766D5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPWFPVKKVR KQMKLLLLLL LLTCAAWLTY VHRSLVRPGR ALRQRLGYGR
60 70 80 90 100
DGEKLTGVTD SRGVRVPSST QRSEDSSESH EEEQAPEGRG PNMLFPGGPR
110 120 130 140 150
KPPPLNLTHQ TPPWREEFKG QVNLHVFEDW CGGAVGHLRR NLHFPLFPHT
160 170 180 190 200
RTTVTKLAVS PKWKNYGLRI FGFIHPARDG DIQFSVASDD NSEFWLSLDE
210 220 230 240 250
SPAAAQLVAF VGKTGSEWTA PGEFTKFSSQ VSKPRRLMAS RRYYFELLHK
260 270 280 290 300
QDDKGSDHVE VGWRAFLPGL KFEIIDSAHI SLYTDESSLK MDHVAHVPQS
310 320 330 340 350
PASHIGGFPP QGEPSADMLH PDPRDTFFLT PRMEPLSLEN VLEPCAYAPT
360 370 380 390 400
YILKDFPIAR YQGLQFVYLS FIYPNDHTRL THMETDNKCF YRESPLYLER
410 420 430 440 450
FGFYKYMKMD KEEGEEDEEE EVQRRAFLFL NPDDFLDEED EQDLLDSLEP
460 470 480 490 500
TDASVQQSHR TPTPAASTGT TASPTPPTTS PLDEQTLRHS RALNWAPRPL
510 520 530 540 550
PLFLGRAPPP RTVEKSPSKV YVTRVRPGQR ASPRALRDSP WPPFPGVFLR
560 570 580 590 600
PKPLPRVQLR VPPHPPRTQG YRTSGPKVTE LKPPVRAQTS QGGREGQLHG
610 620 630 640 650
QGLMVPTVDL NSSVETQPVT SFLSLSQVSR PQLPGEGEEG EEDGAPGDEA
660 670 680 690 700
TSEDSEEEEE PAAGRPLGRW REDAINWQRT FSVGAMDFEL LRSDWNDLRC
710 720 730 740 750
NVSGNLQLPE AEAVDVVAQY MERLNAKHGG RFSLLRIVNV EKRRDSARGS
760 770 780 790 800
RFLLELELQE RGGSRQRLSE YVFLRLPGAR VGDEDGESPE PPPAASIHPD
810 820 830 840 850
SRPELCRPLH LAWRQDVMVH FIVPVKNQAR WVVQFLADMT ALHVHTGDSY
860 870 880 890 900
FNIILVDFES EDMDVERALR AAQLPRYQYL KRTGNFERSA GLQTGVDAVE
910 920 930 940 950
DPSSIVFLCD LHIHFPPNIL DSIRKHCVEG KLAFAPVVMR LGCGSSPWDP
960 970 980 990 1000
HGYWEVNGFG LFGIYKSDFD RVGGMNTEEF RDQWGGEDWE LLDRVLQAGL
1010 1020 1030
EVERLRLRHF YHHYHSKRGM WATRSRKGAR AQRS
Length:1,034
Mass (Da):117,373
Last modified:July 5, 2004 - v1
Checksum:i2EF36F8710CA5B73
GO
Isoform 2 (identifier: Q766D5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-817: Missing.

Note: No experimental confirmation available.
Show »
Length:217
Mass (Da):25,065
Checksum:i4BAFDA45368D8103
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 817817Missing in isoform 2. 1 PublicationVSP_020917Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB114827 mRNA. Translation: BAD15101.1.
BC038881 mRNA. Translation: AAH38881.1.
CCDSiCCDS21998.1. [Q766D5-1]
RefSeqiNP_808565.2. NM_177897.3. [Q766D5-1]
UniGeneiMm.133741.

Genome annotation databases

EnsembliENSMUST00000048002; ENSMUSP00000039758; ENSMUSG00000055629. [Q766D5-1]
GeneIDi330671.
KEGGimmu:330671.
UCSCiuc009kje.1. mouse. [Q766D5-1]
uc009kjg.1. mouse. [Q766D5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Beta1,4-N-acetylgalactosaminyltransferase IV

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB114827 mRNA. Translation: BAD15101.1.
BC038881 mRNA. Translation: AAH38881.1.
CCDSiCCDS21998.1. [Q766D5-1]
RefSeqiNP_808565.2. NM_177897.3. [Q766D5-1]
UniGeneiMm.133741.

3D structure databases

ProteinModelPortaliQ766D5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000039758.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteiQ766D5.

Proteomic databases

EPDiQ766D5.
MaxQBiQ766D5.
PaxDbiQ766D5.
PeptideAtlasiQ766D5.
PRIDEiQ766D5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048002; ENSMUSP00000039758; ENSMUSG00000055629. [Q766D5-1]
GeneIDi330671.
KEGGimmu:330671.
UCSCiuc009kje.1. mouse. [Q766D5-1]
uc009kjg.1. mouse. [Q766D5-2]

Organism-specific databases

CTDi338707.
MGIiMGI:2652891. B4galnt4.

Phylogenomic databases

eggNOGiKOG3588. Eukaryota.
ENOG410XNYM. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000071102.
HOVERGENiHBG080886.
InParanoidiQ766D5.
KOiK09657.
OMAiGMWATRS.
OrthoDBiEOG7CG6ZB.
PhylomeDBiQ766D5.
TreeFamiTF318303.

Miscellaneous databases

ChiTaRSiB4galnt4. mouse.
PROiQ766D5.
SOURCEiSearch...

Gene expression databases

BgeeiQ766D5.
CleanExiMM_B4GALNT4.
GenevisibleiQ766D5. MM.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
IPR011658. PA14_dom.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N'-diacetyllactosediamine."
    Gotoh M., Sato T., Kiyohara K., Kameyama A., Kikuchi N., Kwon Y.-D., Ishizuka Y., Iwai T., Nakanishi H., Narimatsu H.
    FEBS Lett. 562:134-140(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Mammary gland.

Entry informationi

Entry nameiB4GN4_MOUSE
AccessioniPrimary (citable) accession number: Q766D5
Secondary accession number(s): Q8CHV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: July 5, 2004
Last modified: July 6, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.