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Protein

Aspartic proteinase nepenthesin-1

Gene

nep1

Organism
Nepenthes gracilis (Slender pitcher plant)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Extracellular proteinase found in the pitcher fluid of carnivorous plants. Digest prey for nitrogen uptake.

Catalytic activityi

Similar to pepsin, but also cleaves on either side of Asp and at Lys-|-Arg.

Enzyme regulationi

Inhibited by pepstatin and by diazoacetyl-D,L-norleucine methyl ester (DAN) in the presence of Cu2+ ions.By similarity

pH dependencei

Optimum pH is 2.6. Retains 95% and 79% of the original activity after incubation for 30 days at pH 3.0 and pH 10.0 respectively.

Temperature dependencei

Optimum temperature is 55 degrees Celsius. Thermostable up to 50 degrees Celsius. Retains 60% of the original activity after incubation for 30 days at 50 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei113PROSITE-ProRule annotation1
Active sitei315PROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.040.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic proteinase nepenthesin-1 (EC:3.4.23.12)
Alternative name(s):
Nepenthesin-I
Gene namesi
Name:nep1
OrganismiNepenthes gracilis (Slender pitcher plant)
Taxonomic identifieri150966 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesNepenthaceaeNepenthes

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002591425 – 78Activation peptideBy similarityAdd BLAST54
ChainiPRO_000002591579 – 437Aspartic proteinase nepenthesin-1Add BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi53N-linked (GlcNAc...)Sequence analysis1
Glycosylationi98N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi123 ↔ 126Curated
Disulfide bondi129 ↔ 203Curated
Glycosylationi131N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi150 ↔ 168Curated
Disulfide bondi155 ↔ 163Curated
Glycosylationi198N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi240 ↔ 434Curated
Glycosylationi267N-linked (GlcNAc...)Sequence analysis1
Glycosylationi307N-linked (GlcNAc...)Sequence analysis1
Glycosylationi345N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi354 ↔ 395Curated

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ766C3.
SMRiQ766C3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini95 – 430Peptidase A1PROSITE-ProRule annotationAdd BLAST336

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR032799. TAXi_C.
IPR032861. TAXi_N.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF14541. TAXi_C. 1 hit.
PF14543. TAXi_N. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 1 hit.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q766C3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSLYSFLL ALSIVYIFVA PTHSTSRTAL NHRHEAKVTG FQIMLEHVDS
60 70 80 90 100
GKNLTKFQLL ERAIERGSRR LQRLEAMLNG PSGVETSVYA GDGEYLMNLS
110 120 130 140 150
IGTPAQPFSA IMDTGSDLIW TQCQPCTQCF NQSTPIFNPQ GSSSFSTLPC
160 170 180 190 200
SSQLCQALSS PTCSNNFCQY TYGYGDGSET QGSMGTETLT FGSVSIPNIT
210 220 230 240 250
FGCGENNQGF GQGNGAGLVG MGRGPLSLPS QLDVTKFSYC MTPIGSSTPS
260 270 280 290 300
NLLLGSLANS VTAGSPNTTL IQSSQIPTFY YITLNGLSVG STRLPIDPSA
310 320 330 340 350
FALNSNNGTG GIIIDSGTTL TYFVNNAYQS VRQEFISQIN LPVVNGSSSG
360 370 380 390 400
FDLCFQTPSD PSNLQIPTFV MHFDGGDLEL PSENYFISPS NGLICLAMGS
410 420 430
SSQGMSIFGN IQQQNMLVVY DTGNSVVSFA SAQCGAS
Length:437
Mass (Da):46,357
Last modified:July 5, 2004 - v1
Checksum:iA430C3A84ED3FF3E
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti233D → V in nepenthesin-1b. 1 Publication1
Natural varianti251N → T in nepenthesin-1b. 1 Publication1
Natural varianti392G → E in nepenthesin-1b. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB114914 mRNA. Translation: BAD07474.1.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB114914 mRNA. Translation: BAD07474.1.

3D structure databases

ProteinModelPortaliQ766C3.
SMRiQ766C3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiA01.040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR032799. TAXi_C.
IPR032861. TAXi_N.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF14541. TAXi_C. 1 hit.
PF14543. TAXi_N. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 1 hit.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEP1_NEPGR
AccessioniPrimary (citable) accession number: Q766C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.