Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aspartic proteinase nepenthesin-1

Gene

nep1

Organism
Nepenthes gracilis (Slender pitcher plant)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Extracellular proteinase found in the pitcher fluid of carnivorous plants. Digest prey for nitrogen uptake.

Catalytic activityi

Similar to pepsin, but also cleaves on either side of Asp and at Lys-|-Arg.

Enzyme regulationi

Inhibited by pepstatin and by diazoacetyl-D,L-norleucine methyl ester (DAN) in the presence of Cu2+ ions.By similarity

pH dependencei

Optimum pH is 2.6. Retains 95% and 79% of the original activity after incubation for 30 days at pH 3.0 and pH 10.0 respectively.

Temperature dependencei

Optimum temperature is 55 degrees Celsius. Thermostable up to 50 degrees Celsius. Retains 60% of the original activity after incubation for 30 days at 50 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei113 – 1131PROSITE-ProRule annotation
Active sitei315 – 3151PROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.040.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic proteinase nepenthesin-1 (EC:3.4.23.12)
Alternative name(s):
Nepenthesin-I
Gene namesi
Name:nep1
OrganismiNepenthes gracilis (Slender pitcher plant)
Taxonomic identifieri150966 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesNepenthaceaeNepenthes

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Propeptidei25 – 7854Activation peptideBy similarityPRO_0000025914Add
BLAST
Chaini79 – 437359Aspartic proteinase nepenthesin-1PRO_0000025915Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi53 – 531N-linked (GlcNAc...)Sequence analysis
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence analysis
Disulfide bondi123 ↔ 126Curated
Disulfide bondi129 ↔ 203Curated
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence analysis
Disulfide bondi150 ↔ 168Curated
Disulfide bondi155 ↔ 163Curated
Glycosylationi198 – 1981N-linked (GlcNAc...)Sequence analysis
Disulfide bondi240 ↔ 434Curated
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence analysis
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence analysis
Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence analysis
Disulfide bondi354 ↔ 395Curated

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ766C3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini95 – 430336Peptidase A1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR032799. TAXi_C.
IPR032861. TAXi_N.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF14541. TAXi_C. 1 hit.
PF14543. TAXi_N. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 1 hit.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q766C3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSLYSFLL ALSIVYIFVA PTHSTSRTAL NHRHEAKVTG FQIMLEHVDS
60 70 80 90 100
GKNLTKFQLL ERAIERGSRR LQRLEAMLNG PSGVETSVYA GDGEYLMNLS
110 120 130 140 150
IGTPAQPFSA IMDTGSDLIW TQCQPCTQCF NQSTPIFNPQ GSSSFSTLPC
160 170 180 190 200
SSQLCQALSS PTCSNNFCQY TYGYGDGSET QGSMGTETLT FGSVSIPNIT
210 220 230 240 250
FGCGENNQGF GQGNGAGLVG MGRGPLSLPS QLDVTKFSYC MTPIGSSTPS
260 270 280 290 300
NLLLGSLANS VTAGSPNTTL IQSSQIPTFY YITLNGLSVG STRLPIDPSA
310 320 330 340 350
FALNSNNGTG GIIIDSGTTL TYFVNNAYQS VRQEFISQIN LPVVNGSSSG
360 370 380 390 400
FDLCFQTPSD PSNLQIPTFV MHFDGGDLEL PSENYFISPS NGLICLAMGS
410 420 430
SSQGMSIFGN IQQQNMLVVY DTGNSVVSFA SAQCGAS
Length:437
Mass (Da):46,357
Last modified:July 5, 2004 - v1
Checksum:iA430C3A84ED3FF3E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti233 – 2331D → V in nepenthesin-1b. 1 Publication
Natural varianti251 – 2511N → T in nepenthesin-1b. 1 Publication
Natural varianti392 – 3921G → E in nepenthesin-1b. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB114914 mRNA. Translation: BAD07474.1.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB114914 mRNA. Translation: BAD07474.1.

3D structure databases

ProteinModelPortaliQ766C3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiA01.040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR032799. TAXi_C.
IPR032861. TAXi_N.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF14541. TAXi_C. 1 hit.
PF14543. TAXi_N. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 1 hit.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEP1_NEPGR
AccessioniPrimary (citable) accession number: Q766C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 5, 2004
Last modified: April 13, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.