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Q765N2

- FAS2_LACKL

UniProt

Q765N2 - FAS2_LACKL

Protein

Fatty acid synthase subunit alpha

Gene

FAS2

Organism
Lachancea kluyveri (Yeast) (Saccharomyces kluyveri)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The alpha subunit contains domains for: acyl carrier protein, 3-oxoacyl-[acyl-carrier-protein] reductase, and 3-oxoacyl-[acyl-carrier-protein] synthase. In this species, higher amounts of C18 than C16 fatty acids are produced.1 Publication

    Catalytic activityi

    Acetyl-CoA + n malonyl-CoA + 2n NADPH = long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP+.
    Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].
    (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei1305 – 13051For beta-ketoacyl synthase activityBy similarity
    Metal bindingi1774 – 17741MagnesiumBy similarity
    Metal bindingi1775 – 17751Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi1776 – 17761MagnesiumBy similarity
    Binding sitei1800 – 18001Acetyl-CoABy similarity
    Binding sitei1810 – 18101Acetyl-CoABy similarity
    Metal bindingi1874 – 18741MagnesiumBy similarity
    Metal bindingi1875 – 18751Magnesium; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB-EC
    2. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
    3. fatty-acyl-CoA synthase activity Source: UniProtKB-EC
    4. holo-[acyl-carrier-protein] synthase activity Source: InterPro
    5. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-KW
    2. macromolecule biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase, Transferase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding, NAD, NADP

    Enzyme and pathway databases

    BRENDAi2.3.1.86. 274780.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid synthase subunit alpha (EC:2.3.1.86)
    Alternative name(s):
    p190/210
    Including the following 3 domains:
    Acyl carrier
    3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.100)
    Alternative name(s):
    Beta-ketoacyl reductase
    3-oxoacyl-[acyl-carrier-protein] synthase (EC:2.3.1.41)
    Alternative name(s):
    Beta-ketoacyl synthase
    Gene namesi
    Name:FAS2
    Synonyms:Sk-FAS2
    OrganismiLachancea kluyveri (Yeast) (Saccharomyces kluyveri)
    Taxonomic identifieri4934 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeLachancea

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 18881888Fatty acid synthase subunit alphaPRO_0000419252Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei181 – 1811O-(pantetheine 4'-phosphoryl)serineBy similarity

    Keywords - PTMi

    Phosphopantetheine, Phosphoprotein

    Interactioni

    Subunit structurei

    Fatty acid synthase is composed of alpha and beta subunits.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ765N2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini141 – 302162Acyl carrierAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni675 – 874200Beta-ketoacyl reductaseBy similarityAdd
    BLAST
    Regioni1149 – 1363215Beta-ketoacyl synthaseBy similarityAdd
    BLAST
    Regioni1774 – 17763Acetyl-CoA bindingBy similarity
    Regioni1819 – 183517Acetyl-CoA bindingBy similarityAdd
    BLAST
    Regioni1843 – 18464Acetyl-CoA bindingBy similarity
    Regioni1873 – 18753Acetyl-CoA bindingBy similarity

    Domaini

    The N-terminal ACP and almost all of the KR domains play an important role in determining the carbon chain length of fatty acids produced.1 Publication

    Sequence similaritiesi

    Contains 1 acyl carrier domain.Curated

    Family and domain databases

    Gene3Di3.40.366.10. 1 hit.
    3.40.47.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.470.20. 1 hit.
    HAMAPiMF_00101. AcpS.
    InterProiIPR008278. 4-PPantetheinyl_Trfase_SF.
    IPR001227. Ac_transferase_dom.
    IPR002582. ACPS.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR026025. FAS_alpha_yeast.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016040. NAD(P)-bd_dom.
    IPR004568. PPantethiene-prot_Trfase_dom.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF01648. ACPS. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000454. FAS_yeast_alpha. 1 hit.
    ProDomiPD004282. PPantethiene-prot_Trfase. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF52151. SSF52151. 1 hit.
    SSF53901. SSF53901. 3 hits.
    SSF56214. SSF56214. 1 hit.
    TIGRFAMsiTIGR00556. pantethn_trn. 1 hit.
    PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q765N2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMKPEVEQE LAHVLLTELL AYQFASPVRW IETQDVFLKD FNTERVVEIG     50
    PSPTLAGMAQ RTIKNKYESY DAALSLQRQV LCYSKDAKEI YYTPDPADLA 100
    PVEEPNAEEQ TGAAATPAAA AAPAAAAAAP AAPAARPVAE LPDEAVKASL 150
    LLHVLVAQKL KKSLEQVPMS KTIKDLVGGK STVQNEILGD LGKEFGSTPE 200
    KPEETPLEEL AETFQDTFAG SLGKQSSSLI SRLMSSKMPG GFTITVARKY 250
    LQTRWGLGSG RQDSVLLIAL TNEPASRLGG EADAKSFLDS MAQKYASISG 300
    VDLSSASAGA SAGAGAGGAG GATIDAAAFE ELTKDQKVMA RQQLEVLARY 350
    LKMDLNGGEK KFLQEKNTVA ELQSQLDYLN NELGEYFIQG ISTSFSRKKA 400
    RVFDSSWNWA KQALLTLYFQ IIHGVLKNVD REVVTEAINI MNRSNETLIK 450
    FMEYHISHCD ENNGENYKLA KTLGHQLIEN CKQVLDMDPV YKDISKPTGP 500
    KTNIDKNGNI KYSEEPRAAV RKLSQYVQEM ALGGPLTKES QPTIQEDLTR 550
    VYKAINAQAA EHNISDSTKL EFEKLYGELL KFLETSNEID ASATTRLAGV 600
    VDDDLDKDST KEVASLPNKS EISKNVSSII PRETVPFLHL KKKLPSCEWA 650
    YDRQLSTLFL DGLEKAAING VTFKDKYVLI TGAGAGSIGA EVLQGLVQGG 700
    AKVIVTTSRF SKKVTDYYQS IYAQYGAKGS TLVVVPFNQG SKQDVEALID 750
    FIYDDEKNGG LGWDLDAVIP FAAIPENGIE LDKIDSKSEF AHRIMLTNIL 800
    RMLGSVKKQK SARGIETRPA QVILPLSPNH GTFGGDGMYS ESKLSLETLF 850
    NRWHSESWSN QLTICGAIIG WTRGTGLMNA NNIIAEGIEK MGVRTFSQKE 900
    MAFNLLGLLI PEVVNLCQRS PVMADLNGGL QFLTDLKEFT GKLRGELTET 950
    SEIRKAVSIE TALEHKTVAG ANADAAFAQV EVQPRANIQL EFPTLKPYET 1000
    VKKIGSPDLE GLLDLEKVIV VTGFSEVGPW GSSRTRGEME AFGEFSLEGC 1050
    VEMSWIMGLI KYHNGNLKGR PYTGWVDSKT NEPVDDKDIK AKDESHVLEH 1100
    SGIRLIEPEL FNSYNPEKKQ MIQEVVIEED LEPFEASKET AEQFKHEHGD 1150
    KVDIFEIPET GEFSVRLLKG ATLYIPKALR FDRLVAGQIP TGWNAKTYGI 1200
    SDDTISQVDP ITLFVLVSVI EAFIASGITD PYEMYKYVHV SEVGNCSGSG 1250
    MGGVSALRGM FKDRYKDQPV QNDILQESFI NTMSAWVNML LISSSGPIKT 1300
    PVGACATAVE SLDIGVETIL SGKAKICIVG GYDDFQEEGS YEFGNMNATS 1350
    NSLDEFDHGR TPAEMSRPAT TTRNGFMEAQ GAGIQVIMNA DLALKMGVPI 1400
    YGILALTATA TDKIGRSVPA PGKGILTTAR EHHGNLKFPT PLLDIKYRKR 1450
    QLKNREAQIK QWVETELEML KYEAEGIPAE DQETFYAERT EEIKREATRQ 1500
    LKSAQAQWGS EFYKNDPRIA PLRGALATYG LTIDDLGVAS FHGTSTKAND 1550
    KNESATINEM MKHLGRSEGN PVLGVFQKYL TGHPKGAAGA WMMNGALQIL 1600
    NSGIVPGNRN ADNVDKLLQQ FEYILYPSRS LKTDGIKAVS VTSFGFGQKG 1650
    AQAVAVHPDF LYAAVDEATY NAYVAKVTAR EKAAYKYFHN GMIHNTLFVS 1700
    KEHAPYSDEL EQPVYLDPLA RVSSDKKTGA LVFNGKGIQS SSQFVSEENR 1750
    KTATIVSELA KKTAGTDASG VGVDVELIKS INVENDTFIE RNFTEAEIAY 1800
    CKKQPSVQSS FAGTWSAKEA VFKSLGVKSQ GGGASLKDIE ITREAGKGPE 1850
    VVLHGAAKEA ATKANVKNVK VSISHDDFQS VAVAVSEK 1888
    Length:1,888
    Mass (Da):206,206
    Last modified:July 5, 2004 - v1
    Checksum:i6301BD0A025B7919
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB115969 Genomic DNA. Translation: BAD08376.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB115969 Genomic DNA. Translation: BAD08376.1 .

    3D structure databases

    ProteinModelPortali Q765N2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 2.3.1.86. 274780.

    Family and domain databases

    Gene3Di 3.40.366.10. 1 hit.
    3.40.47.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.470.20. 1 hit.
    HAMAPi MF_00101. AcpS.
    InterProi IPR008278. 4-PPantetheinyl_Trfase_SF.
    IPR001227. Ac_transferase_dom.
    IPR002582. ACPS.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR026025. FAS_alpha_yeast.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016040. NAD(P)-bd_dom.
    IPR004568. PPantethiene-prot_Trfase_dom.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF01648. ACPS. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000454. FAS_yeast_alpha. 1 hit.
    ProDomi PD004282. PPantethiene-prot_Trfase. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF52151. SSF52151. 1 hit.
    SSF53901. SSF53901. 3 hits.
    SSF56214. SSF56214. 1 hit.
    TIGRFAMsi TIGR00556. pantethn_trn. 1 hit.
    PROSITEi PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    PS00012. PHOSPHOPANTETHEINE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional characterization of a fatty acid synthase component FAS2 gene from Saccharomyces kluyveri."
      Oura T., Kajiwara S.
      Curr. Genet. 49:393-402(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DOMAIN.
      Strain: NBRC 1893 / Ks-133(+).

    Entry informationi

    Entry nameiFAS2_LACKL
    AccessioniPrimary (citable) accession number: Q765N2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2012
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3