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Protein

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B

Gene

Mgat5b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase that acts on alpha-linked mannose of N-glycans and O-mannosyl glycans. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to the beta 1-6 linkage of the mannose residue of GlcNAcbeta1,2-Manalpha on both the alpha1,3- and alpha1,6-linked mannose arms in the core structure of N-glycan. Also acts on the GlcNAcbeta1,2-Manalpha1-Ser/Thr moiety, forming a 2,6-branched structure in brain O-mannosyl glycan. Plays an active role in modulating integrin and laminin-dependent adhesion and migration of neuronal cells via its activity in the O-mannosyl glycan pathway (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

GO - Molecular functioni

GO - Biological processi

  • protein N-linked glycosylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Metal-binding

Protein family/group databases

CAZyiGT18. Glycosyltransferase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B (EC:2.4.1.-, EC:2.4.1.155)
Alternative name(s):
Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase B
GlcNAc-T Vb
Short name:
GNT-Vb
Mannoside acetylglucosaminyltransferase 5B
N-acetylglucosaminyl-transferase Vb
N-acetylglucosaminyltransferase IX
Short name:
GNT-IX
Gene namesi
Name:Mgat5b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:3606200. Mgat5b.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
Transmembranei25 – 45Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini46 – 792LumenalSequence analysisAdd BLAST747

GO - Cellular componenti

  • Golgi apparatus Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002886121 – 792Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase BAdd BLAST792

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi127N-linked (GlcNAc...)Sequence analysis1
Glycosylationi675N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ765H6.
PRIDEiQ765H6.

PTM databases

iPTMnetiQ765H6.
PhosphoSitePlusiQ765H6.

Expressioni

Tissue specificityi

Present in brain (at protein level). Predominantly expressed in cerebrum and cerebellum.1 Publication

Gene expression databases

BgeeiENSMUSG00000043857.
ExpressionAtlasiQ765H6. baseline and differential.
GenevisibleiQ765H6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099316.

Structurei

3D structure databases

ProteinModelPortaliQ765H6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 18 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IDYS. Eukaryota.
ENOG410XTV7. LUCA.
GeneTreeiENSGT00390000012263.
HOGENOMiHOG000006557.
HOVERGENiHBG052469.
InParanoidiQ765H6.
KOiK09661.
OMAiDHGLICE.
OrthoDBiEOG091G044F.
PhylomeDBiQ765H6.
TreeFamiTF313714.

Family and domain databases

InterProiIPR026116. GlyclTrfase_18.
[Graphical view]
PANTHERiPTHR15075. PTHR15075. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q765H6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MITVNPDGKI MVRRCLVTLR PFRLFVLGIG FFTLCFLMTS LGGQFSARRL
60 70 80 90 100
GDSPFTIRTE VPGSPESRGA LRKMSDLLEL MVKRMDMLAR LENSSELHRT
110 120 130 140 150
ASVAHLAADR LTPGASLIER IQAIAQNVSD IAVKVDQILR HSLILHSKVS
160 170 180 190 200
EGRRDQCEAP SDPKFPDCSG KVEWMRARWT SDPCYAFFGV DGTECSFLIY
210 220 230 240 250
LSEVEWFCPP LPWRNQTAAR TAPKSLPRVQ AVFRSNLSHL LELMGSGKES
260 270 280 290 300
LIFMKKRTRR FTAQWTKAAK YLAQKLGDIR RDQKQILVHI GFLTEESGDV
310 320 330 340 350
FSPRVLKGGP LGEMVQWADI LAALYVLGHS LRITVSLKEL QSNLGVPPGR
360 370 380 390 400
GNCPLTVPLP FDLIYTDYHG LQQMKQHMGL SFKKYRCRIR VIDTFGTEPA
410 420 430 440 450
YNHEEYATLH GYRTNWGYWN LNPKQFMTMF PHTPDNSFMG FVSEELNETE
460 470 480 490 500
KQLIKDGKAS NMAVVYGKEA SIWKLQGKEK FLAVLNKYME IHGTVYYESQ
510 520 530 540 550
RPPEVPAFVK NHGLLPQPEF QQLLRKAKLF IGFGFPYEGP APLEAIANGC
560 570 580 590 600
IFLQSRFSPP HSSLNHEFFR GKPTSREVFS QHPYAENFIG KPHVWTVDYN
610 620 630 640 650
NSDEFETAIK AIMNTQVDPY LPYEYTCAGM LERINAYIQH QDFCVGPSPL
660 670 680 690 700
PPGASTAQSP FVLAPNATHL EWAQNISSVP GAWPPTHSLR AWLAAPGRAC
710 720 730 740 750
TDACLDHGLI CEPSFFPFLN SQNSFLKLQV PCDSTEWEMH HLYPAFAQPG
760 770 780 790
QECYLQKEPL LFSCAGASTK YQRLCPCRDF RKGQVALCQG CL
Length:792
Mass (Da):89,473
Last modified:July 5, 2004 - v1
Checksum:iC063E2614AB240B1
GO
Isoform 2 (identifier: Q765H6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-377: Missing.
     378-431: MGLSFKKYRC...NPKQFMTMFP → MALGVPCSEL...FFPLCGRAVA
     475-476: Missing.

Note: No experimental confirmation available.
Show »
Length:413
Mass (Da):45,964
Checksum:i38262458C3AE6A0E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0257361 – 377Missing in isoform 2. 1 PublicationAdd BLAST377
Alternative sequenceiVSP_025737378 – 431MGLSF…MTMFP → MALGVPCSELQASPPMGALP TRSAETPGRGISREQASAWT FDNLFFPLCGRAVA in isoform 2. 1 PublicationAdd BLAST54
Alternative sequenceiVSP_025738475 – 476Missing in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB116028 mRNA. Translation: BAD08454.1.
AB119127 mRNA. Translation: BAC87707.1.
AK049266 mRNA. Translation: BAC33645.1.
AL627205, AL663089 Genomic DNA. Translation: CAM23854.1.
AL663089, AL627205 Genomic DNA. Translation: CAM23089.1.
BC094604 mRNA. Translation: AAH94604.1.
CCDSiCCDS25682.1. [Q765H6-1]
RefSeqiNP_766536.2. NM_172948.3. [Q765H6-1]
UniGeneiMm.35141.

Genome annotation databases

EnsembliENSMUST00000103027; ENSMUSP00000099316; ENSMUSG00000043857. [Q765H6-1]
GeneIDi268510.
KEGGimmu:268510.
UCSCiuc007mmu.1. mouse. [Q765H6-1]
uc007mmv.1. mouse. [Q765H6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB116028 mRNA. Translation: BAD08454.1.
AB119127 mRNA. Translation: BAC87707.1.
AK049266 mRNA. Translation: BAC33645.1.
AL627205, AL663089 Genomic DNA. Translation: CAM23854.1.
AL663089, AL627205 Genomic DNA. Translation: CAM23089.1.
BC094604 mRNA. Translation: AAH94604.1.
CCDSiCCDS25682.1. [Q765H6-1]
RefSeqiNP_766536.2. NM_172948.3. [Q765H6-1]
UniGeneiMm.35141.

3D structure databases

ProteinModelPortaliQ765H6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099316.

Protein family/group databases

CAZyiGT18. Glycosyltransferase Family 18.

PTM databases

iPTMnetiQ765H6.
PhosphoSitePlusiQ765H6.

Proteomic databases

PaxDbiQ765H6.
PRIDEiQ765H6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103027; ENSMUSP00000099316; ENSMUSG00000043857. [Q765H6-1]
GeneIDi268510.
KEGGimmu:268510.
UCSCiuc007mmu.1. mouse. [Q765H6-1]
uc007mmv.1. mouse. [Q765H6-2]

Organism-specific databases

CTDi146664.
MGIiMGI:3606200. Mgat5b.

Phylogenomic databases

eggNOGiENOG410IDYS. Eukaryota.
ENOG410XTV7. LUCA.
GeneTreeiENSGT00390000012263.
HOGENOMiHOG000006557.
HOVERGENiHBG052469.
InParanoidiQ765H6.
KOiK09661.
OMAiDHGLICE.
OrthoDBiEOG091G044F.
PhylomeDBiQ765H6.
TreeFamiTF313714.

Miscellaneous databases

PROiQ765H6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000043857.
ExpressionAtlasiQ765H6. baseline and differential.
GenevisibleiQ765H6. MM.

Family and domain databases

InterProiIPR026116. GlyclTrfase_18.
[Graphical view]
PANTHERiPTHR15075. PTHR15075. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiMGT5B_MOUSE
AccessioniPrimary (citable) accession number: Q765H6
Secondary accession number(s): Q8C7T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.