Reviewed, UniProtKB/Swiss-Prot Q765H6 (MGT5B_MOUSE)

Names and origin

Protein names

Recommended name:
    Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B
    EC=2.4.1.155
    EC=2.4.1.-
Alternative name(s):
    Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase B
    Mannoside acetylglucosaminyltransferase 5B
    N-acetylglucosaminyl-transferase Vb
    GlcNAc-T Vb
      Short name=GNT-Vb
    N-acetylglucosaminyltransferase IX
      Short name=GNT-IX

Gene names

Name:

Mgat5b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	792 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Glycosyltransferase that acts on alpha-linked mannose of N-glycans and O-mannosyl glycans. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to the beta 1-6 linkage of the mannose residue of GlcNAcbeta1,2-Manalpha on both the alpha1,3- and alpha1,6-linked mannose arms in the core structure of N-glycan. Also acts on the GlcNAcbeta1,2-Manalpha1-Ser/Thr moiety, forming a 2,6-branched structure in brain O-mannosyl glycan. Plays an active role in modulating integrin and laminin-dependent adhesion and migration of neuronal cells via its activity in the O-mannosyl glycan pathway By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Divalent metal cations.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein. Ref.2
Tissue specificity	Present in brain (at protein level). Predominantly expressed in cerebrum and cerebellum. Ref.2
Sequence similarities	Belongs to the glycosyltransferase 18 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q765H6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q765H6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-377: Missing.
     378-431: MGLSFKKYRC...NPKQFMTMFP → MALGVPCSEL...FFPLCGRAVA
     475-476: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 792	792	Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B		PRO_0000288612
Regions
	Topological domain	1 – 24	24	Cytoplasmic Potential
	Transmembrane	25 – 45	21	Signal-anchor for type II membrane protein Potential
	Topological domain	46 – 792	747	Lumenal Potential
Amino acid modifications
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	675	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 377	377	Missing in isoform 2.		VSP_025736
	Alternative sequence	378 – 431	54	MGLSF…MTMFP → MALGVPCSELQASPPMGALP TRSAETPGRGISREQASAWT FDNLFFPLCGRAVA in isoform 2.		VSP_025737
	Alternative sequence	475 – 476	2	Missing in isoform 2.		VSP_025738

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C063E2614AB240B1	FASTA	792	89,473
	10 20 30 40 50 60 MITVNPDGKI MVRRCLVTLR PFRLFVLGIG FFTLCFLMTS LGGQFSARRL GDSPFTIRTE 70 80 90 100 110 120 VPGSPESRGA LRKMSDLLEL MVKRMDMLAR LENSSELHRT ASVAHLAADR LTPGASLIER 130 140 150 160 170 180 IQAIAQNVSD IAVKVDQILR HSLILHSKVS EGRRDQCEAP SDPKFPDCSG KVEWMRARWT 190 200 210 220 230 240 SDPCYAFFGV DGTECSFLIY LSEVEWFCPP LPWRNQTAAR TAPKSLPRVQ AVFRSNLSHL 250 260 270 280 290 300 LELMGSGKES LIFMKKRTRR FTAQWTKAAK YLAQKLGDIR RDQKQILVHI GFLTEESGDV 310 320 330 340 350 360 FSPRVLKGGP LGEMVQWADI LAALYVLGHS LRITVSLKEL QSNLGVPPGR GNCPLTVPLP 370 380 390 400 410 420 FDLIYTDYHG LQQMKQHMGL SFKKYRCRIR VIDTFGTEPA YNHEEYATLH GYRTNWGYWN 430 440 450 460 470 480 LNPKQFMTMF PHTPDNSFMG FVSEELNETE KQLIKDGKAS NMAVVYGKEA SIWKLQGKEK 490 500 510 520 530 540 FLAVLNKYME IHGTVYYESQ RPPEVPAFVK NHGLLPQPEF QQLLRKAKLF IGFGFPYEGP 550 560 570 580 590 600 APLEAIANGC IFLQSRFSPP HSSLNHEFFR GKPTSREVFS QHPYAENFIG KPHVWTVDYN 610 620 630 640 650 660 NSDEFETAIK AIMNTQVDPY LPYEYTCAGM LERINAYIQH QDFCVGPSPL PPGASTAQSP 670 680 690 700 710 720 FVLAPNATHL EWAQNISSVP GAWPPTHSLR AWLAAPGRAC TDACLDHGLI CEPSFFPFLN 730 740 750 760 770 780 SQNSFLKLQV PCDSTEWEMH HLYPAFAQPG QECYLQKEPL LFSCAGASTK YQRLCPCRDF 790 RKGQVALCQG CL
	Isoform 2. Checksum: 38262458C3AE6A0E	FASTA	413	45,964
	10 20 30 40 50 60 MALGVPCSEL QASPPMGALP TRSAETPGRG ISREQASAWT FDNLFFPLCG RAVAHTPDNS 70 80 90 100 110 120 FMGFVSEELN ETEKQLIKDG KASNMAVVYG KEASIWKGKE KFLAVLNKYM EIHGTVYYES 130 140 150 160 170 180 QRPPEVPAFV KNHGLLPQPE FQQLLRKAKL FIGFGFPYEG PAPLEAIANG CIFLQSRFSP 190 200 210 220 230 240 PHSSLNHEFF RGKPTSREVF SQHPYAENFI GKPHVWTVDY NNSDEFETAI KAIMNTQVDP 250 260 270 280 290 300 YLPYEYTCAG MLERINAYIQ HQDFCVGPSP LPPGASTAQS PFVLAPNATH LEWAQNISSV 310 320 330 340 350 360 PGAWPPTHSL RAWLAAPGRA CTDACLDHGL ICEPSFFPFL NSQNSFLKLQ VPCDSTEWEM 370 380 390 400 410 HHLYPAFAQP GQECYLQKEP LLFSCAGAST KYQRLCPCRD FRKGQVALCQ GCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel beta(1,6)-N-acetylglucosaminyltransferase V (GnT-VB)." Kaneko M., Alvarez-Manilla G., Kamar M., Lee I., Lee J.-K., Troupe K., Zhang W., Osawa M., Pierce M. FEBS Lett. 554:515-519(2003) [PubMed: 14623122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Demonstration of the expression and the enzymatic activity of N-acetylglucosaminyltransferase IX in the mouse brain." Inamori K., Mita S., Gu J., Mizuno-Horikawa Y., Miyoshi E., Dennis J.W., Taniguchi N. Biochim. Biophys. Acta 1760:678-684(2006) [PubMed: 16413118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Embryonic stem cell.
[4]	The mouse genome sequencing consortium Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Embryonic brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB116028 mRNA. Translation: BAD08454.1. AB119127 mRNA. Translation: BAC87707.1. AK049266 mRNA. Translation: BAC33645.1. AL627205, AL663089 Genomic DNA. Translation: CAM23854.1. AL663089, AL627205 Genomic DNA. Translation: CAM23089.1. BC094604 mRNA. Translation: AAH94604.1.
IPI	IPI00420293. IPI00466941.
RefSeq	NP_766536.2.
UniGene	Mm.35141
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT18. Glycosyltransferase Family 18.
PTM databases
PhosphoSite	Q765H6.
Proteomic databases
PRIDE	Q765H6.
Genome annotation databases
Ensembl	ENSMUSG00000043857. Mus musculus. [Contig view]
GeneID	268510.
KEGG	mmu:268510.
Organism-specific databases
MGI	MGI:3606200. Mgat5b.
Phylogenomic databases
HOGENOM	Q765H6.
HOVERGEN	Q765H6.
OMA	Q765H6. TESEMNH.
Enzyme and pathway databases
BRENDA	2.4.1.155. 244.
Gene expression databases
ArrayExpress	Q765H6.
Bgee	Q765H6.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	392349.
SOURCE	Search...

Entry information

Entry name

MGT5B_MOUSE

Accession

Primary (citable) accession number: Q765H6
Secondary accession number(s): Q8C7T8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)