Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q765A7

- PGAP1_RAT

UniProt

Q765A7 - PGAP1_RAT

Protein

GPI inositol-deacylase

Gene

Pgap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei174 – 1741

    GO - Molecular functioni

    1. nuclease activity Source: UniProtKB
    2. phosphoric ester hydrolase activity Source: UniProtKB

    GO - Biological processi

    1. anterior/posterior axis specification Source: Ensembl
    2. embryonic pattern specification Source: Ensembl
    3. forebrain regionalization Source: Ensembl
    4. GPI anchor biosynthetic process Source: RGD
    5. head development Source: Ensembl
    6. intracellular protein transport Source: InterPro
    7. myo-inositol transport Source: UniProtKB
    8. nucleic acid phosphodiester bond hydrolysis Source: GOC
    9. sensory perception of sound Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GPI inositol-deacylase (EC:3.1.-.-)
    Alternative name(s):
    Post-GPI attachment to proteins factor 1
    Gene namesi
    Name:Pgap1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi1303213. Pgap1.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi174 – 1741S → A: Abolishes the inositol deacylation of GPI-anchor proteins. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 922922GPI inositol-deacylasePRO_0000277625Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi363 – 3631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi558 – 5581N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ765A7.
    PRIDEiQ765A7.

    Expressioni

    Gene expression databases

    GenevestigatoriQ765A7.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000017967.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – 597565LumenalSequence AnalysisAdd
    BLAST
    Topological domaini619 – 64123CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini663 – 6686LumenalSequence Analysis
    Topological domaini690 – 6945CytoplasmicSequence Analysis
    Topological domaini716 – 73318LumenalSequence AnalysisAdd
    BLAST
    Topological domaini755 – 81662CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini838 – 85316LumenalSequence AnalysisAdd
    BLAST
    Topological domaini875 – 89420CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini916 – 9227LumenalSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei598 – 61821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei642 – 66221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei669 – 68921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei695 – 71521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei734 – 75421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei817 – 83721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei854 – 87421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei895 – 91521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GPI inositol-deacylase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG312611.
    GeneTreeiENSGT00390000016484.
    HOGENOMiHOG000090006.
    HOVERGENiHBG082122.
    InParanoidiQ765A7.
    KOiK05294.
    OMAiTKFVHEC.
    OrthoDBiEOG7KM5RZ.
    PhylomeDBiQ765A7.
    TreeFamiTF314565.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR012908. PGAP1-like.
    [Graphical view]
    PfamiPF07819. PGAP1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00120. LIPASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q765A7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLHSVNLWN LAFYVFMVFL ATLGLWDVFF GFEENKCSMS YMFEYPEYQK    50
    IELPKKLTKR YPAYELYLYG EGSYAEEHKI LPLTGIPVLF LPGNAGSYKQ 100
    VRSIGSIALR KAEDIDFKYH FDFFSVNFNE ELVALYGGSL QKQTKFVHEC 150
    IKAILKLYKG QEFPPTSVAI IGHSMGGLVA RALLTLKNFK QDLINLLVTQ 200
    ATPHVAPVMP LDRFITEFYM TVNNYWILNA RHINLTTLSV AGGFRDYQVR 250
    SGLTFLPTLS HHTSALSVVT SAVPKTWVST DHLSIVWCKQ LQLTTIRAFF 300
    DLIDADTKQI TQKSKKKLSV LNHHFIRHPA KQFEENPSII SDLTGTSMWV 350
    PVKVSRWSYV AYNESDKIYF AFPLANHRKV YTHAYCQSTM LDTNSWIFGC 400
    INSTSMCRQG VDLSWKAELL PTIKSLTLRL QDYPSLSHIV VYVPSVHGSK 450
    FVVDCEFFKK EARSIQLPVT HLFSFGLSSR KAIINTSGRY YNIELLNLGQ 500
    IYQAFKVNVV SKCTGGKEEI TSIYKLHIPW SYEDSLTIAQ VPSSVDISLK 550
    LHVAQPENDS HVALLKMYTS SDCQYEVTIK TSFPQILGQV VRFHGGALPA 600
    YVVSSILLAY GGQLYSLLST GFCLEYGTML DKEAKPYKVD PFVIMIKFLL 650
    GYKWFKELWD AVLLPELDAI VLTSQSMCFP LVSLILFLFG TCTAYWSGLL 700
    SSASVQLLSS LWLALKRPAE LPKDVKVMSP DLPVLTVVFL IISWTTCGAL 750
    AILLSYLYYV FKVVHLQASL TTFKNNQPVN PKHSRRSEKK SNHHKDSAIQ 800
    NPRLSANDAE DSLRMHSTVI NLLTWVVLLS MPSLIYWSKN LRYYFKLNPD 850
    PCKPLAFLLI PAIAVLGNTH TVSIKSSKLL KTASQFPLPL AVGVIAFGSS 900
    HLYRVPCFVI IPLVFHSLCN FM 922
    Length:922
    Mass (Da):104,384
    Last modified:July 5, 2004 - v1
    Checksum:iB07E02DF99875FD3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB116149 mRNA. Translation: BAD08353.1.
    RefSeqiNP_973719.1. NM_201990.1.
    UniGeneiRn.205923.

    Genome annotation databases

    EnsembliENSRNOT00000017967; ENSRNOP00000017967; ENSRNOG00000013388.
    GeneIDi316400.
    KEGGirno:316400.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB116149 mRNA. Translation: BAD08353.1 .
    RefSeqi NP_973719.1. NM_201990.1.
    UniGenei Rn.205923.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000017967.

    Proteomic databases

    PaxDbi Q765A7.
    PRIDEi Q765A7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000017967 ; ENSRNOP00000017967 ; ENSRNOG00000013388 .
    GeneIDi 316400.
    KEGGi rno:316400.

    Organism-specific databases

    CTDi 80055.
    RGDi 1303213. Pgap1.

    Phylogenomic databases

    eggNOGi NOG312611.
    GeneTreei ENSGT00390000016484.
    HOGENOMi HOG000090006.
    HOVERGENi HBG082122.
    InParanoidi Q765A7.
    KOi K05294.
    OMAi TKFVHEC.
    OrthoDBi EOG7KM5RZ.
    PhylomeDBi Q765A7.
    TreeFami TF314565.

    Miscellaneous databases

    NextBioi 670811.
    PROi Q765A7.

    Gene expression databases

    Genevestigatori Q765A7.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR012908. PGAP1-like.
    [Graphical view ]
    Pfami PF07819. PGAP1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00120. LIPASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Inositol deacylation of glycosylphosphatidylinositol-anchored proteins is mediated by mammalian PGAP1 and yeast Bst1p."
      Tanaka S., Maeda Y., Tashima Y., Kinoshita T.
      J. Biol. Chem. 279:14256-14263(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF SER-174.

    Entry informationi

    Entry nameiPGAP1_RAT
    AccessioniPrimary (citable) accession number: Q765A7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3