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Q765A7 (PGAP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GPI inositol-deacylase

EC=3.1.-.-
Alternative name(s):
Post-GPI attachment to proteins factor 1
Gene names
Name:Pgap1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length922 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. Ref.1

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.

Sequence similarities

Belongs to the GPI inositol-deacylase family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGPI anchor biosynthetic process

Inferred from mutant phenotype Ref.1. Source: RGD

anterior/posterior axis specification

Inferred from electronic annotation. Source: Ensembl

embryonic pattern specification

Inferred from electronic annotation. Source: Ensembl

forebrain regionalization

Inferred from electronic annotation. Source: Ensembl

head development

Inferred from electronic annotation. Source: Ensembl

intracellular protein transport

Inferred from electronic annotation. Source: InterPro

myo-inositol transport

Inferred from direct assay Ref.1. Source: UniProtKB

nucleic acid phosphodiester bond hydrolysis

Inferred from direct assay Ref.1. Source: GOC

sensory perception of sound

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentendoplasmic reticulum

Inferred from direct assay Ref.1. Source: UniProtKB

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionnuclease activity

Inferred from direct assay Ref.1. Source: UniProtKB

phosphoric ester hydrolase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 922922GPI inositol-deacylase
PRO_0000277625

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3221Helical; Potential
Topological domain33 – 597565Lumenal Potential
Transmembrane598 – 61821Helical; Potential
Topological domain619 – 64123Cytoplasmic Potential
Transmembrane642 – 66221Helical; Potential
Topological domain663 – 6686Lumenal Potential
Transmembrane669 – 68921Helical; Potential
Topological domain690 – 6945Cytoplasmic Potential
Transmembrane695 – 71521Helical; Potential
Topological domain716 – 73318Lumenal Potential
Transmembrane734 – 75421Helical; Potential
Topological domain755 – 81662Cytoplasmic Potential
Transmembrane817 – 83721Helical; Potential
Topological domain838 – 85316Lumenal Potential
Transmembrane854 – 87421Helical; Potential
Topological domain875 – 89420Cytoplasmic Potential
Transmembrane895 – 91521Helical; Potential
Topological domain916 – 9227Lumenal Potential

Sites

Active site1741

Amino acid modifications

Glycosylation3631N-linked (GlcNAc...) Potential
Glycosylation4021N-linked (GlcNAc...) Potential
Glycosylation5581N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis1741S → A: Abolishes the inositol deacylation of GPI-anchor proteins. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q765A7 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: B07E02DF99875FD3

FASTA922104,384
        10         20         30         40         50         60 
MFLHSVNLWN LAFYVFMVFL ATLGLWDVFF GFEENKCSMS YMFEYPEYQK IELPKKLTKR 

        70         80         90        100        110        120 
YPAYELYLYG EGSYAEEHKI LPLTGIPVLF LPGNAGSYKQ VRSIGSIALR KAEDIDFKYH 

       130        140        150        160        170        180 
FDFFSVNFNE ELVALYGGSL QKQTKFVHEC IKAILKLYKG QEFPPTSVAI IGHSMGGLVA 

       190        200        210        220        230        240 
RALLTLKNFK QDLINLLVTQ ATPHVAPVMP LDRFITEFYM TVNNYWILNA RHINLTTLSV 

       250        260        270        280        290        300 
AGGFRDYQVR SGLTFLPTLS HHTSALSVVT SAVPKTWVST DHLSIVWCKQ LQLTTIRAFF 

       310        320        330        340        350        360 
DLIDADTKQI TQKSKKKLSV LNHHFIRHPA KQFEENPSII SDLTGTSMWV PVKVSRWSYV 

       370        380        390        400        410        420 
AYNESDKIYF AFPLANHRKV YTHAYCQSTM LDTNSWIFGC INSTSMCRQG VDLSWKAELL 

       430        440        450        460        470        480 
PTIKSLTLRL QDYPSLSHIV VYVPSVHGSK FVVDCEFFKK EARSIQLPVT HLFSFGLSSR 

       490        500        510        520        530        540 
KAIINTSGRY YNIELLNLGQ IYQAFKVNVV SKCTGGKEEI TSIYKLHIPW SYEDSLTIAQ 

       550        560        570        580        590        600 
VPSSVDISLK LHVAQPENDS HVALLKMYTS SDCQYEVTIK TSFPQILGQV VRFHGGALPA 

       610        620        630        640        650        660 
YVVSSILLAY GGQLYSLLST GFCLEYGTML DKEAKPYKVD PFVIMIKFLL GYKWFKELWD 

       670        680        690        700        710        720 
AVLLPELDAI VLTSQSMCFP LVSLILFLFG TCTAYWSGLL SSASVQLLSS LWLALKRPAE 

       730        740        750        760        770        780 
LPKDVKVMSP DLPVLTVVFL IISWTTCGAL AILLSYLYYV FKVVHLQASL TTFKNNQPVN 

       790        800        810        820        830        840 
PKHSRRSEKK SNHHKDSAIQ NPRLSANDAE DSLRMHSTVI NLLTWVVLLS MPSLIYWSKN 

       850        860        870        880        890        900 
LRYYFKLNPD PCKPLAFLLI PAIAVLGNTH TVSIKSSKLL KTASQFPLPL AVGVIAFGSS 

       910        920 
HLYRVPCFVI IPLVFHSLCN FM 

« Hide

References

[1]"Inositol deacylation of glycosylphosphatidylinositol-anchored proteins is mediated by mammalian PGAP1 and yeast Bst1p."
Tanaka S., Maeda Y., Tashima Y., Kinoshita T.
J. Biol. Chem. 279:14256-14263(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF SER-174.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB116149 mRNA. Translation: BAD08353.1.
RefSeqNP_973719.1. NM_201990.1.
UniGeneRn.205923.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000017967.

Proteomic databases

PaxDbQ765A7.
PRIDEQ765A7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000017967; ENSRNOP00000017967; ENSRNOG00000013388.
GeneID316400.
KEGGrno:316400.

Organism-specific databases

CTD80055.
RGD1303213. Pgap1.

Phylogenomic databases

eggNOGNOG312611.
GeneTreeENSGT00390000016484.
HOGENOMHOG000090006.
HOVERGENHBG082122.
InParanoidQ765A7.
KOK05294.
OMATKFVHEC.
OrthoDBEOG7KM5RZ.
PhylomeDBQ765A7.
TreeFamTF314565.

Gene expression databases

GenevestigatorQ765A7.

Family and domain databases

InterProIPR012908. PGAP1-like.
[Graphical view]
PfamPF07819. PGAP1. 1 hit.
[Graphical view]
PROSITEPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio670811.
PROQ765A7.

Entry information

Entry namePGAP1_RAT
AccessionPrimary (citable) accession number: Q765A7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families