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Protein
Submitted name:

Integrase

Gene
N/A
Organism
Human immunodeficiency virus 1
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).SAAS annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

EndonucleaseSAAS annotation, Hydrolase, Nuclease, Nucleotidyltransferase, RNA-directed DNA polymeraseSAAS annotation, Transferase

Keywords - Biological processi

DNA integrationSAAS annotation

Keywords - Ligandi

DNA-bindingSAAS annotation, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Submitted name:
IntegraseImported
OrganismiHuman immunodeficiency virus 1Imported
Taxonomic identifieri11676 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusPrimate lentivirus group
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HDDC2Q7Z4H32EBI-6248077,EBI-6163836From a different organism.
HEATR1Q9H5833EBI-6248077,EBI-1048716From a different organism.
MCM6Q145662EBI-6248077,EBI-374900From a different organism.
PDIA4P136673EBI-6248077,EBI-1054653From a different organism.
PLAAQ9Y2633EBI-6248077,EBI-1994037From a different organism.
RANBP6O605182EBI-6248077,EBI-1055771From a different organism.
UBA1P223142EBI-6248077,EBI-709688From a different organism.
YWHABP319463EBI-6248077,EBI-359815From a different organism.

Protein-protein interaction databases

IntActiQ76353. 25 interactions.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HYVX-ray1.70A47-212[»]
1HYZX-ray2.30A47-212[»]
3ZCMX-ray1.80A/B56-212[»]
3ZSOX-ray1.75A/B56-212[»]
3ZSQX-ray1.70A/B56-212[»]
3ZSRX-ray1.70A/B56-212[»]
3ZSVX-ray1.75A/B56-212[»]
3ZSWX-ray1.80A/B56-212[»]
3ZSXX-ray1.95A/B56-212[»]
3ZSYX-ray2.20A/B56-212[»]
3ZSZX-ray2.00A/B56-212[»]
3ZT0X-ray1.95A/B56-212[»]
3ZT1X-ray1.75A/B56-212[»]
3ZT2X-ray1.70A/B56-212[»]
3ZT3X-ray1.95A/B56-212[»]
3ZT4X-ray2.20A/B56-212[»]
4CE9X-ray2.10A/B50-212[»]
4CEAX-ray1.80A/B50-212[»]
4CEBX-ray1.75A/B50-212[»]
4CECX-ray1.75A/B50-212[»]
4CEDX-ray1.75A/B50-212[»]
4CEEX-ray1.80A/B50-212[»]
4CEFX-ray1.70A/B50-212[»]
4CEOX-ray1.90A/B50-212[»]
4CEQX-ray1.70A/B50-212[»]
4CERX-ray1.70A/B50-212[»]
4CESX-ray1.85A/B50-212[»]
4CEZX-ray1.70A/B50-212[»]
4CF0X-ray1.85A/B50-212[»]
4CF1X-ray1.95A/B50-212[»]
4CF2X-ray1.95A/B50-212[»]
4CF8X-ray1.65A/B50-212[»]
4CF9X-ray2.10A/B50-212[»]
4CFAX-ray2.05A/B50-212[»]
4CFBX-ray1.95A/B50-212[»]
4CFCX-ray1.90A/B50-212[»]
4CFDX-ray2.15A/B50-212[»]
4CGDX-ray2.00A/B50-212[»]
4CGFX-ray1.70A/B50-212[»]
4CGGX-ray1.75A/B50-212[»]
4CGHX-ray1.76A/B50-212[»]
4CGIX-ray2.07A/B50-212[»]
4CGJX-ray2.15A/B50-212[»]
4CHNX-ray2.00A/B50-212[»]
4CHOX-ray1.70A/B50-212[»]
4CHPX-ray1.90A/B50-212[»]
4CHQX-ray1.95A/B50-212[»]
4CHYX-ray1.70A/B50-212[»]
4CHZX-ray1.80A/B50-212[»]
4CIEX-ray1.70A/B50-212[»]
4CIFX-ray1.80A/B50-212[»]
4CIGX-ray1.70A/B50-212[»]
4CJ3X-ray1.70A/B50-212[»]
4CJ4X-ray1.80A/B50-212[»]
4CJ5X-ray1.95A/B50-212[»]
4CJEX-ray1.90A/B50-212[»]
4CJFX-ray1.90A/B50-212[»]
4CJKX-ray1.75A/B50-212[»]
4CJLX-ray1.77A50-212[»]
4CJPX-ray2.00A/B50-212[»]
4CJQX-ray1.70A/B50-212[»]
4CJRX-ray1.80A/B50-212[»]
4CJSX-ray1.80A/B50-212[»]
4CJTX-ray1.71A/B50-212[»]
4CJUX-ray1.70A/B50-212[»]
4CJVX-ray1.95A/B50-212[»]
4CJWX-ray1.95A/B50-212[»]
4CK1X-ray1.75A/B50-212[»]
4CK2X-ray1.85A/B50-212[»]
4CK3X-ray1.79A/B50-212[»]
4LH4X-ray1.80A50-212[»]
4LH5X-ray2.19A50-212[»]
4OVLX-ray1.70A/B50-212[»]
4Y1CX-ray2.30A/B50-212[»]
ProteinModelPortaliQ76353.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ76353.

Family & Domainsi

Sequence similaritiesi

Contains RNase H domain.SAAS annotation
Contains integrase catalytic domain.SAAS annotation
Contains integrase-type DNA-binding domain.SAAS annotation
Contains integrase-type zinc finger.SAAS annotation
Contains reverse transcriptase domain.SAAS annotation

Keywords - Domaini

Zinc-fingerSAAS annotation

Family and domain databases

Gene3Di1.10.10.200. 1 hit.
2.30.30.10. 1 hit.
3.30.420.10. 1 hit.
InterProiIPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00665. rve. 1 hit.
[Graphical view]
SUPFAMiSSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q76353-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
FLDGIDKAQE EHEKYHSNWR AMASDFNLPP VVAKEIVASC DKCQLKGEAM
60 70 80 90 100
HGQVDCSPGI WQLDCTHLEG KVILVAVHVA SGYIEAEVIP AETGQETAYF
110 120 130 140 150
LLKLAGRWPV KTVHTDNGSN FTSTTVKAAC WWAGIKQEFG IPYNPQSQGV
160 170 180 190 200
IESMNKELKK IIGQVRDQAE HLKTAVQMAV FIHNFKRKGG IGGYSAGERI
210 220 230 240 250
VDIIATDIQT KELQKQITKI QNFRVYYRDS RDPVWKGPAK LLWKGEGAVV
260 270 280
IQDNSDIKVV PRRKAKIIRD YGKQMAGDDC VASRQDED
Length:288
Mass (Da):32,199
Last modified:November 1, 1996 - v1
Checksum:i0680E5CD1E201580
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11Imported

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21188 Unassigned RNA. Translation: AAC37875.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21188 Unassigned RNA. Translation: AAC37875.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HYVX-ray1.70A47-212[»]
1HYZX-ray2.30A47-212[»]
3ZCMX-ray1.80A/B56-212[»]
3ZSOX-ray1.75A/B56-212[»]
3ZSQX-ray1.70A/B56-212[»]
3ZSRX-ray1.70A/B56-212[»]
3ZSVX-ray1.75A/B56-212[»]
3ZSWX-ray1.80A/B56-212[»]
3ZSXX-ray1.95A/B56-212[»]
3ZSYX-ray2.20A/B56-212[»]
3ZSZX-ray2.00A/B56-212[»]
3ZT0X-ray1.95A/B56-212[»]
3ZT1X-ray1.75A/B56-212[»]
3ZT2X-ray1.70A/B56-212[»]
3ZT3X-ray1.95A/B56-212[»]
3ZT4X-ray2.20A/B56-212[»]
4CE9X-ray2.10A/B50-212[»]
4CEAX-ray1.80A/B50-212[»]
4CEBX-ray1.75A/B50-212[»]
4CECX-ray1.75A/B50-212[»]
4CEDX-ray1.75A/B50-212[»]
4CEEX-ray1.80A/B50-212[»]
4CEFX-ray1.70A/B50-212[»]
4CEOX-ray1.90A/B50-212[»]
4CEQX-ray1.70A/B50-212[»]
4CERX-ray1.70A/B50-212[»]
4CESX-ray1.85A/B50-212[»]
4CEZX-ray1.70A/B50-212[»]
4CF0X-ray1.85A/B50-212[»]
4CF1X-ray1.95A/B50-212[»]
4CF2X-ray1.95A/B50-212[»]
4CF8X-ray1.65A/B50-212[»]
4CF9X-ray2.10A/B50-212[»]
4CFAX-ray2.05A/B50-212[»]
4CFBX-ray1.95A/B50-212[»]
4CFCX-ray1.90A/B50-212[»]
4CFDX-ray2.15A/B50-212[»]
4CGDX-ray2.00A/B50-212[»]
4CGFX-ray1.70A/B50-212[»]
4CGGX-ray1.75A/B50-212[»]
4CGHX-ray1.76A/B50-212[»]
4CGIX-ray2.07A/B50-212[»]
4CGJX-ray2.15A/B50-212[»]
4CHNX-ray2.00A/B50-212[»]
4CHOX-ray1.70A/B50-212[»]
4CHPX-ray1.90A/B50-212[»]
4CHQX-ray1.95A/B50-212[»]
4CHYX-ray1.70A/B50-212[»]
4CHZX-ray1.80A/B50-212[»]
4CIEX-ray1.70A/B50-212[»]
4CIFX-ray1.80A/B50-212[»]
4CIGX-ray1.70A/B50-212[»]
4CJ3X-ray1.70A/B50-212[»]
4CJ4X-ray1.80A/B50-212[»]
4CJ5X-ray1.95A/B50-212[»]
4CJEX-ray1.90A/B50-212[»]
4CJFX-ray1.90A/B50-212[»]
4CJKX-ray1.75A/B50-212[»]
4CJLX-ray1.77A50-212[»]
4CJPX-ray2.00A/B50-212[»]
4CJQX-ray1.70A/B50-212[»]
4CJRX-ray1.80A/B50-212[»]
4CJSX-ray1.80A/B50-212[»]
4CJTX-ray1.71A/B50-212[»]
4CJUX-ray1.70A/B50-212[»]
4CJVX-ray1.95A/B50-212[»]
4CJWX-ray1.95A/B50-212[»]
4CK1X-ray1.75A/B50-212[»]
4CK2X-ray1.85A/B50-212[»]
4CK3X-ray1.79A/B50-212[»]
4LH4X-ray1.80A50-212[»]
4LH5X-ray2.19A50-212[»]
4OVLX-ray1.70A/B50-212[»]
4Y1CX-ray2.30A/B50-212[»]
ProteinModelPortaliQ76353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ76353. 25 interactions.

Chemistry

ChEMBLiCHEMBL2366505.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ76353.

Family and domain databases

Gene3Di1.10.10.200. 1 hit.
2.30.30.10. 1 hit.
3.30.420.10. 1 hit.
InterProiIPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00665. rve. 1 hit.
[Graphical view]
SUPFAMiSSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "HIV1 integrase expressed in Escherichia coli from a synthetic gene."
    Holler T.P., Foltin S.K., Ye Q.Z., Hupe D.J.
    Gene 136:323-328(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: NY5Imported.
  2. "Identification of a small-molecule binding site at the dimer interface of the HIV integrase catalytic domain."
    Molteni V., Greenwald J., Rhodes D., Hwang Y., Kwiatkowski W., Bushman F.D., Siegel J.S., Choe S.
    Acta Crystallogr. D 57:536-544(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 47-212.
  3. "Small molecule inhibitors of the LEDGF site of human immunodeficiency virus integrase identified by fragment screening and structure based design."
    Peat T.S., Rhodes D.I., Vandegraaff N., Le G., Smith J.A., Clark L.J., Jones E.D., Coates J.A., Thienthong N., Newman J., Dolezal O., Mulder R., Ryan J.H., Savage G.P., Francis C.L., Deadman J.J.
    PLoS ONE 7:e40147-e40147(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 56-212.
  4. "Dual inhibition of HIV-1 replication by integrase-LEDGF allosteric inhibitors is predominant at the post-integration stage."
    Le Rouzic E., Bonnard D., Chasset S., Bruneau J.M., Chevreuil F., Le Strat F., Nguyen J., Beauvoir R., Amadori C., Brias J., Vomscheid S., Eiler S., Levy N., Delelis O., Deprez E., Saib A., Zamborlini A., Emiliani S.
    , Ruff M., Ledoussal B., Moreau F., Benarous R.
    Retrovirology 10:144-144(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 50-212.
  5. "Interrogating HIV integrase for compounds that bind--a SAMPL challenge."
    Peat T.S., Dolezal O., Newman J., Mobley D., Deadman J.J.
    J. Comput. Aided Mol. Des. 28:347-362(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 50-212.
  6. "HEXAPEPTIDE MIMETICS OF LEDGF IN COMPLEX WITH HIV-1 INTEGRASE."
    Northfield S.E., Wielens J., Headey S.J., Mulcair M., Scanlon M.J., Parker M.W., Thompson P.E., Chalmers D.K.
    Submitted (FEB-2015) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 50-212.

Entry informationi

Entry nameiQ76353_9HIV1
AccessioniPrimary (citable) accession number: Q76353
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: April 29, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.