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Q76353

- Q76353_9HIV1

UniProt

Q76353 - Q76353_9HIV1

Protein
Submitted name:

Integrase

Gene
N/A
Organism
Human immunodeficiency virus 1
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).SAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei65 – 651Chloride 1; via amide nitrogenImported
    Binding sitei67 – 671Acetate 1Imported
    Binding sitei67 – 671Sulfate 2Imported
    Binding sitei71 – 711Sulfate 3Imported
    Binding sitei87 – 871Acetate 1Imported
    Binding sitei87 – 871Acetate 7Imported
    Binding sitei87 – 871Acetate 8Imported
    Binding sitei87 – 871Acetate 9
    Binding sitei87 – 871Sulfate 6Imported
    Binding sitei92 – 921Acetate 1; via amide nitrogenImported
    Binding sitei92 – 921Chloride 2; via amide nitrogenImported
    Binding sitei92 – 921Chloride 3; via amide nitrogenImported
    Binding sitei92 – 921Sulfate 2; via amide nitrogenImported
    Binding sitei95 – 951Acetate 10; via amide nitrogenImported
    Binding sitei95 – 951Sulfate 3Imported
    Binding sitei95 – 951Sulfate 7Imported
    Binding sitei99 – 991Acetate 11Imported
    Binding sitei99 – 991Acetate 12Imported
    Binding sitei99 – 991Acetate 8Imported
    Binding sitei99 – 991Sulfate 9Imported
    Binding sitei103 – 1031Acetate 11Imported
    Binding sitei103 – 1031Acetate 12Imported
    Binding sitei103 – 1031Acetate 8Imported
    Binding sitei103 – 1031Sulfate 6Imported
    Binding sitei103 – 1031Sulfate 9Imported
    Binding sitei107 – 1071Acetate 12Imported
    Binding sitei107 – 1071Acetate 13; via carbonyl oxygenImported
    Binding sitei107 – 1071Acetate 17Imported
    Binding sitei107 – 1071Acetate 4Imported
    Binding sitei107 – 1071Acetate 6Imported
    Binding sitei107 – 1071Sulfate 12Imported
    Binding sitei107 – 1071Sulfate 3Imported
    Binding sitei107 – 1071Sulfate 5Imported
    Binding sitei107 – 1071Sulfate 7Imported
    Binding sitei107 – 1071Sulfate 8Imported
    Binding sitei125 – 1251Acetate 10Imported
    Binding sitei159 – 1591Sulfate 1Imported
    Binding sitei166 – 1661Sulfate 3Imported
    Binding sitei172 – 1721Sulfate 15; via amide nitrogenImported
    Binding sitei184 – 1841Acetate 15; via carbonyl oxygenImported
    Binding sitei184 – 1841Acetate 16; via carbonyl oxygenImported
    Binding sitei184 – 1841Sulfate 4; via carbonyl oxygenImported
    Binding sitei197 – 1971Acetate 2; via carbonyl oxygenImported

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. endonuclease activity Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. RNA-directed DNA polymerase activity Source: UniProtKB-KW
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. DNA integration Source: UniProtKB-KW

    Keywords - Molecular functioni

    EndonucleaseSAAS annotation, Hydrolase, Nuclease, Nucleotidyltransferase, RNA-directed DNA polymeraseSAAS annotation, Transferase

    Keywords - Biological processi

    DNA integrationSAAS annotation

    Keywords - Ligandi

    DNA-bindingSAAS annotation, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    IntegraseImported
    OrganismiHuman immunodeficiency virus 1Imported
    Taxonomic identifieri11676 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusPrimate lentivirus group
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HDDC2Q7Z4H32EBI-6248077,EBI-6163836From a different organism.
    HEATR1Q9H5833EBI-6248077,EBI-1048716From a different organism.
    MCM6Q145662EBI-6248077,EBI-374900From a different organism.
    PDIA4P136673EBI-6248077,EBI-1054653From a different organism.
    PLAAQ9Y2633EBI-6248077,EBI-1994037From a different organism.
    RANBP6O605182EBI-6248077,EBI-1055771From a different organism.
    UBA1P223142EBI-6248077,EBI-709688From a different organism.
    YWHABP319463EBI-6248077,EBI-359815From a different organism.

    Protein-protein interaction databases

    IntActiQ76353. 25 interactions.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HYVX-ray1.70A47-212[»]
    1HYZX-ray2.30A47-212[»]
    3ZCMX-ray1.80A/B56-212[»]
    3ZSOX-ray1.75A/B56-212[»]
    3ZSQX-ray1.70A/B56-212[»]
    3ZSRX-ray1.70A/B56-212[»]
    3ZSVX-ray1.75A/B56-212[»]
    3ZSWX-ray1.80A/B56-212[»]
    3ZSXX-ray1.95A/B56-212[»]
    3ZSYX-ray2.20A/B56-212[»]
    3ZSZX-ray2.00A/B56-212[»]
    3ZT0X-ray1.95A/B56-212[»]
    3ZT1X-ray1.75A/B56-212[»]
    3ZT2X-ray1.70A/B56-212[»]
    3ZT3X-ray1.95A/B56-212[»]
    3ZT4X-ray2.20A/B56-212[»]
    4CE9X-ray2.10A/B50-212[»]
    4CEAX-ray1.80A/B50-212[»]
    4CEBX-ray1.75A/B50-212[»]
    4CECX-ray1.75A/B50-212[»]
    4CEDX-ray1.75A/B50-212[»]
    4CEEX-ray1.80A/B50-212[»]
    4CEFX-ray1.70A/B50-212[»]
    4CEOX-ray1.90A/B50-212[»]
    4CEQX-ray1.70A/B50-212[»]
    4CERX-ray1.70A/B50-212[»]
    4CESX-ray1.85A/B50-212[»]
    4CEZX-ray1.70A/B50-212[»]
    4CF0X-ray1.85A/B50-212[»]
    4CF1X-ray1.95A/B50-212[»]
    4CF2X-ray1.95A/B50-212[»]
    4CF8X-ray1.65A/B50-212[»]
    4CF9X-ray2.10A/B50-212[»]
    4CFAX-ray2.05A/B50-212[»]
    4CFBX-ray1.95A/B50-212[»]
    4CFCX-ray1.90A/B50-212[»]
    4CFDX-ray2.15A/B50-212[»]
    4CGDX-ray2.00A/B50-212[»]
    4CGFX-ray1.70A/B50-212[»]
    4CGGX-ray1.75A/B50-212[»]
    4CGHX-ray1.76A/B50-212[»]
    4CGIX-ray2.07A/B50-212[»]
    4CGJX-ray2.15A/B50-212[»]
    4CHNX-ray2.00A/B50-212[»]
    4CHOX-ray1.70A/B50-212[»]
    4CHPX-ray1.90A/B50-212[»]
    4CHQX-ray1.95A/B50-212[»]
    4CHYX-ray1.70A/B50-212[»]
    4CHZX-ray1.80A/B50-212[»]
    4CIEX-ray1.70A/B50-212[»]
    4CIFX-ray1.80A/B50-212[»]
    4CIGX-ray1.70A/B50-212[»]
    4CJ3X-ray1.70A/B50-212[»]
    4CJ4X-ray1.80A/B50-212[»]
    4CJ5X-ray1.95A/B50-212[»]
    4CJEX-ray1.90A/B50-212[»]
    4CJFX-ray1.90A/B50-212[»]
    4CJKX-ray1.75A/B50-212[»]
    4CJLX-ray1.77A50-212[»]
    4CJPX-ray2.00A/B50-212[»]
    4CJQX-ray1.70A/B50-212[»]
    4CJRX-ray1.80A/B50-212[»]
    4CJSX-ray1.80A/B50-212[»]
    4CJTX-ray1.71A/B50-212[»]
    4CJUX-ray1.70A/B50-212[»]
    4CJVX-ray1.95A/B50-212[»]
    4CJWX-ray1.95A/B50-212[»]
    4CK1X-ray1.75A/B50-212[»]
    4CK2X-ray1.85A/B50-212[»]
    4CK3X-ray1.79A/B50-212[»]
    4LH4X-ray1.80A50-212[»]
    4LH5X-ray2.19A50-212[»]
    4OVLX-ray1.70A/B50-212[»]
    ProteinModelPortaliQ76353.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ76353.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni66 – 672Sulfate 1 bindingImported
    Regioni85 – 873Acetate 4 bindingImported
    Regioni85 – 873Acetate 5 bindingImported
    Regioni85 – 873Acetate 6 bindingImported
    Regioni85 – 873Sulfate 3 binding
    Regioni85 – 873Sulfate 5 bindingImported
    Regioni99 – 1035Acetate 4 bindingImported
    Regioni100 – 1078Acetate 5 bindingImported
    Regioni100 – 1034Acetate 6 bindingImported
    Regioni100 – 1034Sulfate 3 binding
    Regioni100 – 1034Sulfate 5 bindingImported
    Regioni105 – 1095Sulfate 10 bindingImported
    Regioni105 – 1084Sulfate 11 bindingImported
    Regioni106 – 1094Acetate 15 bindingImported
    Regioni106 – 1094Acetate 16 bindingImported
    Regioni107 – 1093Acetate 2 bindingImported
    Regioni123 – 1253Acetate 18 bindingImported
    Regioni123 – 1253Sulfate 10 bindingImported
    Regioni123 – 1253Sulfate 13 bindingImported
    Regioni123 – 1253Sulfate 7 bindingImported
    Regioni123 – 1253Sulfate 8 bindingImported
    Regioni170 – 1745Sulfate 14 bindingImported
    Regioni171 – 1744Chloride 5 bindingImported
    Regioni171 – 1722Sulfate 3 bindingImported

    Sequence similaritiesi

    Contains RNase H domain.SAAS annotation
    Contains integrase catalytic domain.SAAS annotation
    Contains integrase-type DNA-binding domain.SAAS annotation
    Contains integrase-type zinc finger.SAAS annotation
    Contains reverse transcriptase domain.SAAS annotation

    Keywords - Domaini

    Zinc-fingerSAAS annotation

    Family and domain databases

    Gene3Di1.10.10.200. 1 hit.
    2.30.30.10. 1 hit.
    3.30.420.10. 1 hit.
    InterProiIPR001037. Integrase_C_retrovir.
    IPR001584. Integrase_cat-core.
    IPR017856. Integrase_Zn-bd_dom-like_N.
    IPR003308. Integrase_Zn-bd_dom_N.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00552. IN_DBD_C. 1 hit.
    PF02022. Integrase_Zn. 1 hit.
    PF00665. rve. 1 hit.
    [Graphical view]
    SUPFAMiSSF46919. SSF46919. 1 hit.
    SSF50122. SSF50122. 1 hit.
    SSF53098. SSF53098. 1 hit.
    PROSITEiPS50994. INTEGRASE. 1 hit.
    PS51027. INTEGRASE_DBD. 1 hit.
    PS50876. ZF_INTEGRASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Q76353-1 [UniParc]FASTAAdd to Basket

    « Hide

    FLDGIDKAQE EHEKYHSNWR AMASDFNLPP VVAKEIVASC DKCQLKGEAM    50
    HGQVDCSPGI WQLDCTHLEG KVILVAVHVA SGYIEAEVIP AETGQETAYF 100
    LLKLAGRWPV KTVHTDNGSN FTSTTVKAAC WWAGIKQEFG IPYNPQSQGV 150
    IESMNKELKK IIGQVRDQAE HLKTAVQMAV FIHNFKRKGG IGGYSAGERI 200
    VDIIATDIQT KELQKQITKI QNFRVYYRDS RDPVWKGPAK LLWKGEGAVV 250
    IQDNSDIKVV PRRKAKIIRD YGKQMAGDDC VASRQDED 288
    Length:288
    Mass (Da):32,199
    Last modified:November 1, 1996 - v1
    Checksum:i0680E5CD1E201580
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11Imported

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L21188 Unassigned RNA. Translation: AAC37875.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L21188 Unassigned RNA. Translation: AAC37875.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HYV X-ray 1.70 A 47-212 [» ]
    1HYZ X-ray 2.30 A 47-212 [» ]
    3ZCM X-ray 1.80 A/B 56-212 [» ]
    3ZSO X-ray 1.75 A/B 56-212 [» ]
    3ZSQ X-ray 1.70 A/B 56-212 [» ]
    3ZSR X-ray 1.70 A/B 56-212 [» ]
    3ZSV X-ray 1.75 A/B 56-212 [» ]
    3ZSW X-ray 1.80 A/B 56-212 [» ]
    3ZSX X-ray 1.95 A/B 56-212 [» ]
    3ZSY X-ray 2.20 A/B 56-212 [» ]
    3ZSZ X-ray 2.00 A/B 56-212 [» ]
    3ZT0 X-ray 1.95 A/B 56-212 [» ]
    3ZT1 X-ray 1.75 A/B 56-212 [» ]
    3ZT2 X-ray 1.70 A/B 56-212 [» ]
    3ZT3 X-ray 1.95 A/B 56-212 [» ]
    3ZT4 X-ray 2.20 A/B 56-212 [» ]
    4CE9 X-ray 2.10 A/B 50-212 [» ]
    4CEA X-ray 1.80 A/B 50-212 [» ]
    4CEB X-ray 1.75 A/B 50-212 [» ]
    4CEC X-ray 1.75 A/B 50-212 [» ]
    4CED X-ray 1.75 A/B 50-212 [» ]
    4CEE X-ray 1.80 A/B 50-212 [» ]
    4CEF X-ray 1.70 A/B 50-212 [» ]
    4CEO X-ray 1.90 A/B 50-212 [» ]
    4CEQ X-ray 1.70 A/B 50-212 [» ]
    4CER X-ray 1.70 A/B 50-212 [» ]
    4CES X-ray 1.85 A/B 50-212 [» ]
    4CEZ X-ray 1.70 A/B 50-212 [» ]
    4CF0 X-ray 1.85 A/B 50-212 [» ]
    4CF1 X-ray 1.95 A/B 50-212 [» ]
    4CF2 X-ray 1.95 A/B 50-212 [» ]
    4CF8 X-ray 1.65 A/B 50-212 [» ]
    4CF9 X-ray 2.10 A/B 50-212 [» ]
    4CFA X-ray 2.05 A/B 50-212 [» ]
    4CFB X-ray 1.95 A/B 50-212 [» ]
    4CFC X-ray 1.90 A/B 50-212 [» ]
    4CFD X-ray 2.15 A/B 50-212 [» ]
    4CGD X-ray 2.00 A/B 50-212 [» ]
    4CGF X-ray 1.70 A/B 50-212 [» ]
    4CGG X-ray 1.75 A/B 50-212 [» ]
    4CGH X-ray 1.76 A/B 50-212 [» ]
    4CGI X-ray 2.07 A/B 50-212 [» ]
    4CGJ X-ray 2.15 A/B 50-212 [» ]
    4CHN X-ray 2.00 A/B 50-212 [» ]
    4CHO X-ray 1.70 A/B 50-212 [» ]
    4CHP X-ray 1.90 A/B 50-212 [» ]
    4CHQ X-ray 1.95 A/B 50-212 [» ]
    4CHY X-ray 1.70 A/B 50-212 [» ]
    4CHZ X-ray 1.80 A/B 50-212 [» ]
    4CIE X-ray 1.70 A/B 50-212 [» ]
    4CIF X-ray 1.80 A/B 50-212 [» ]
    4CIG X-ray 1.70 A/B 50-212 [» ]
    4CJ3 X-ray 1.70 A/B 50-212 [» ]
    4CJ4 X-ray 1.80 A/B 50-212 [» ]
    4CJ5 X-ray 1.95 A/B 50-212 [» ]
    4CJE X-ray 1.90 A/B 50-212 [» ]
    4CJF X-ray 1.90 A/B 50-212 [» ]
    4CJK X-ray 1.75 A/B 50-212 [» ]
    4CJL X-ray 1.77 A 50-212 [» ]
    4CJP X-ray 2.00 A/B 50-212 [» ]
    4CJQ X-ray 1.70 A/B 50-212 [» ]
    4CJR X-ray 1.80 A/B 50-212 [» ]
    4CJS X-ray 1.80 A/B 50-212 [» ]
    4CJT X-ray 1.71 A/B 50-212 [» ]
    4CJU X-ray 1.70 A/B 50-212 [» ]
    4CJV X-ray 1.95 A/B 50-212 [» ]
    4CJW X-ray 1.95 A/B 50-212 [» ]
    4CK1 X-ray 1.75 A/B 50-212 [» ]
    4CK2 X-ray 1.85 A/B 50-212 [» ]
    4CK3 X-ray 1.79 A/B 50-212 [» ]
    4LH4 X-ray 1.80 A 50-212 [» ]
    4LH5 X-ray 2.19 A 50-212 [» ]
    4OVL X-ray 1.70 A/B 50-212 [» ]
    ProteinModelPortali Q76353.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q76353. 25 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q76353.

    Family and domain databases

    Gene3Di 1.10.10.200. 1 hit.
    2.30.30.10. 1 hit.
    3.30.420.10. 1 hit.
    InterProi IPR001037. Integrase_C_retrovir.
    IPR001584. Integrase_cat-core.
    IPR017856. Integrase_Zn-bd_dom-like_N.
    IPR003308. Integrase_Zn-bd_dom_N.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00552. IN_DBD_C. 1 hit.
    PF02022. Integrase_Zn. 1 hit.
    PF00665. rve. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46919. SSF46919. 1 hit.
    SSF50122. SSF50122. 1 hit.
    SSF53098. SSF53098. 1 hit.
    PROSITEi PS50994. INTEGRASE. 1 hit.
    PS51027. INTEGRASE_DBD. 1 hit.
    PS50876. ZF_INTEGRASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "HIV1 integrase expressed in Escherichia coli from a synthetic gene."
      Holler T.P., Foltin S.K., Ye Q.Z., Hupe D.J.
      Gene 136:323-328(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: NY5Imported.
    2. "Identification of a small-molecule binding site at the dimer interface of the HIV integrase catalytic domain."
      Molteni V., Greenwald J., Rhodes D., Hwang Y., Kwiatkowski W., Bushman F.D., Siegel J.S., Choe S.
      Acta Crystallogr. D 57:536-544(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 47-212.
    3. "Small molecule inhibitors of the LEDGF site of human immunodeficiency virus integrase identified by fragment screening and structure based design."
      Peat T.S., Rhodes D.I., Vandegraaff N., Le G., Smith J.A., Clark L.J., Jones E.D., Coates J.A., Thienthong N., Newman J., Dolezal O., Mulder R., Ryan J.H., Savage G.P., Francis C.L., Deadman J.J.
      PLoS ONE 7:e40147-e40147(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 56-212.
    4. "Dual inhibition of HIV-1 replication by integrase-LEDGF allosteric inhibitors is predominant at the post-integration stage."
      Le Rouzic E., Bonnard D., Chasset S., Bruneau J.M., Chevreuil F., Le Strat F., Nguyen J., Beauvoir R., Amadori C., Brias J., Vomscheid S., Eiler S., Levy N., Delelis O., Deprez E., Saib A., Zamborlini A., Emiliani S.
      , Ruff M., Ledoussal B., Moreau F., Benarous R.
      Retrovirology 10:144-144(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 50-212.
    5. "Interrogating HIV integrase for compounds that bind--a SAMPL challenge."
      Peat T.S., Dolezal O., Newman J., Mobley D., Deadman J.J.
      J. Comput. Aided Mol. Des. 28:347-362(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 50-212.

    Entry informationi

    Entry nameiQ76353_9HIV1
    AccessioniPrimary (citable) accession number: Q76353
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 1, 1996
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3