Q76353 (Q76353_9HIV1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Integrase EMBL AAC37875.1
Organism	Human immunodeficiency virus 1 EMBL AAC37875.1
Taxonomic identifier	11676 [NCBI]
Taxonomic lineage	Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Lentivirus › Primate lentivirus group
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	288 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). SAAS SAAS003308
Sequence similarities	Contains 1 RNase H domain. SAAS SAAS003308 Contains 1 integrase catalytic domain. SAAS SAAS003308 Contains 1 integrase-type DNA-binding domain. SAAS SAAS001584 Contains 1 integrase-type zinc finger. SAAS SAAS001584 Contains 1 reverse transcriptase domain. SAAS SAAS003308

Ontologies

Keywords
Biological process	DNA integration SAAS SAAS001037
Domain	Zinc-finger SAAS SAAS001584
Ligand	DNA-binding SAAS SAAS001584 Metal-binding Zinc
Molecular function	Endonuclease SAAS SAAS003308 Hydrolase Nuclease Nucleotidyltransferase RNA-directed DNA polymerase SAAS SAAS003308 Transferase
Technical term	3D-structure PDB 3ZT4 PDB 3ZCM PDB 3ZSO PDB 1HYV PDB 3ZSQ PDB 3ZSR PDB 3ZSV PDB 3ZSW PDB 3ZSX PDB 3ZSY PDB 3ZSZ PDB 3ZT0 PDB 3ZT1 PDB 3ZT2 PDB 1HYZ PDB 3ZT3
Gene Ontology (GO)
Biological_process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HDDC2	Q7Z4H3	2	EBI-6248077,EBI-6163836	From a different organism.
HEATR1	Q9H583	3	EBI-6248077,EBI-1048716	From a different organism.
MCM6	Q14566	2	EBI-6248077,EBI-374900	From a different organism.
PDIA4	P13667	3	EBI-6248077,EBI-1054653	From a different organism.
PLAA	Q9Y263	3	EBI-6248077,EBI-1994037	From a different organism.
RANBP6	O60518	2	EBI-6248077,EBI-1055771	From a different organism.
UBA1	P22314	2	EBI-6248077,EBI-709688	From a different organism.
YWHAB	P31946	3	EBI-6248077,EBI-359815	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Experimental info
	Non-terminal residue	1	1	EMBL AAC37875.1

Sequences

	Sequence		Length	Mass (Da)
	Q76353 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0680E5CD1E201580	FASTA	288	32,199
	10 20 30 40 50 60 FLDGIDKAQE EHEKYHSNWR AMASDFNLPP VVAKEIVASC DKCQLKGEAM HGQVDCSPGI 70 80 90 100 110 120 WQLDCTHLEG KVILVAVHVA SGYIEAEVIP AETGQETAYF LLKLAGRWPV KTVHTDNGSN 130 140 150 160 170 180 FTSTTVKAAC WWAGIKQEFG IPYNPQSQGV IESMNKELKK IIGQVRDQAE HLKTAVQMAV 190 200 210 220 230 240 FIHNFKRKGG IGGYSAGERI VDIIATDIQT KELQKQITKI QNFRVYYRDS RDPVWKGPAK 250 260 270 280 LLWKGEGAVV IQDNSDIKVV PRRKAKIIRD YGKQMAGDDC VASRQDED

References

[1]	"HIV1 integrase expressed in Escherichia coli from a synthetic gene." Holler T.P., Foltin S.K., Ye Q.Z., Hupe D.J. Gene 136:323-328(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: NY5 EMBL AAC37875.1.
[2]	"Identification of a small-molecule binding site at the dimer interface of the HIV integrase catalytic domain." Molteni V., Greenwald J., Rhodes D., Hwang Y., Kwiatkowski W., Bushman F.D., Siegel J.S., Choe S. Acta Crystallogr. D 57:536-544(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 50-212.
[3]	"Small molecule inhibitors of the LEDGF site of human immunodeficiency virus integrase identified by fragment screening and structure based design." Peat T.S., Rhodes D.I., Vandegraaff N., Le G., Smith J.A., Clark L.J., Jones E.D., Coates J.A., Thienthong N., Newman J., Dolezal O., Mulder R., Ryan J.H., Savage G.P., Francis C.L., Deadman J.J. PLoS ONE 7:e40147-e40147(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 57-212.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L21188 Unassigned RNA. Translation: AAC37875.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HYV	X-ray	1.70	A	50-212	[»]
1HYZ	X-ray	2.30	A	50-212	[»]
3ZCM	X-ray	1.80	A/B	57-212	[»]
3ZSO	X-ray	1.75	A/B	57-212	[»]
3ZSQ	X-ray	1.70	A/B	57-212	[»]
3ZSR	X-ray	1.70	A/B	57-212	[»]
3ZSV	X-ray	1.75	A/B	57-212	[»]
3ZSW	X-ray	1.80	A/B	57-212	[»]
3ZSX	X-ray	1.95	A/B	57-212	[»]
3ZSY	X-ray	2.20	A/B	57-212	[»]
3ZSZ	X-ray	2.00	A/B	57-212	[»]
3ZT0	X-ray	1.95	A/B	57-212	[»]
3ZT1	X-ray	1.75	A/B	57-212	[»]
3ZT2	X-ray	1.70	A/B	57-212	[»]
3ZT3	X-ray	1.95	A/B	57-212	[»]
3ZT4	X-ray	2.20	A/B	57-212	[»]

ProteinModelPortal

Q76353.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q76353. 25 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.10.200. 1 hit.
2.30.30.10. 1 hit.

InterPro

IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR012337. RNaseH-like_dom.
[Graphical view]

Pfam

PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00665. rve. 1 hit.
[Graphical view]

SUPFAM

SSF50122. Integrase_C. 1 hit.
SSF46919. Integrase_Zn_N. 1 hit.
SSF53098. RNaseH_fold. 1 hit.

PROSITE

PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q76353.

Entry information

Entry name

Q76353_9HIV1

Accession

Primary (citable) accession number: Q76353

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)