Reviewed, UniProtKB/Swiss-Prot Q762H5 (MHPB_RHORH)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
    EC=1.13.11.16

Gene names

Name:	mhpB
Synonyms:	bphC3

Organism

Rhodococcus rhodochrous

Taxonomic identifier

1829 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP MF_01653
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653 2,3-dihydroxicinnamic acid + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity. HAMAP MF_01653
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity. HAMAP MF_01653
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653		PRO_0000337666
Sites
	Active site	115	1	Proton donor By similarity
	Active site	180	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q762H5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E3CD5EEABC8A294E	FASTA	316	34,533
	10 20 30 40 50 60 MTQALLCMSH SPLLDHATPP AEVKSAVDDA FARARDFVEA FDPELVVNFG PDHFNGFFYD 70 80 90 100 110 120 LMPPFCIGYR AHGTGDYDSF AGDLDVPEDV AADLAQFVLD HGSDVAISRH MEVDHGAVQP 130 140 150 160 170 180 MEILHGGDAG ARPILPVFVN SIARPFVPMS RVRAFGHAVG DFFAGTDKRV LFLGSGGLSH 190 200 210 220 230 240 DPPVPQFATA TASQREFLTS GRNPPPEARP ARQARTIETA RRFAAGEAEI MDLNPEWDRA 250 260 270 280 290 300 FLDVCRSGRV EDFDRYTADE MDAAAGHSSH EVRTWVAAYS ALRACGEYDV TYEFYRPIKE 310 YIAGFAVTTA QLAGEA

References

[1]

"Multiplicity of 2,3-dihydroxybiphenyl dioxygenase genes in the Gram-positive polychlorinated biphenyl degrading bacterium Rhodococcus rhodochrous K37." Taguchi K., Motoyama M., Kudo T. Biosci. Biotechnol. Biochem. 68:787-795(2004) [PubMed: 15118304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB117721 Genomic DNA. Translation: BAD10895.1.
3D structure databases
SMR	Q762H5. Positions 5-305.
ModBase	Search...
Family and domain databases
HAMAP	MF_01653. MhpB. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB_RHORH

Accession

Primary (citable) accession number: Q762H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)