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Protein

Sodium- and chloride-dependent glycine transporter 2

Gene

Slc6a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi208Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi210Sodium 2; via carbonyl oxygenBy similarity1
Metal bindingi211Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi215Sodium 2By similarity1
Metal bindingi479Sodium 2By similarity1
Metal bindingi511Sodium 2By similarity1
Metal bindingi576Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi579Sodium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Symport, Transport

Keywords - Ligandi

Metal-binding, Sodium

Protein family/group databases

TCDBi2.A.22.2.6. the neurotransmitter:sodium symporter (nss) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent glycine transporter 2
Short name:
GlyT-2
Short name:
GlyT2
Alternative name(s):
Solute carrier family 6 member 5
Gene namesi
Name:Slc6a5Imported
Synonyms:Glyt2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:105090. Slc6a5.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 201CytoplasmicSequence analysisAdd BLAST201
Transmembranei202 – 222Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei229 – 249Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei273 – 293Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini294 – 396ExtracellularSequence analysisAdd BLAST103
Transmembranei397 – 417Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei433 – 453Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei472 – 492Helical; Name=6Sequence analysisAdd BLAST21
Transmembranei510 – 530Helical; Name=7Sequence analysisAdd BLAST21
Transmembranei565 – 585Helical; Name=8Sequence analysisAdd BLAST21
Transmembranei611 – 631Helical; Name=9Sequence analysisAdd BLAST21
Transmembranei640 – 660Helical; Name=10Sequence analysisAdd BLAST21
Transmembranei677 – 697Helical; Name=11Sequence analysisAdd BLAST21
Transmembranei717 – 737Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini738 – 799CytoplasmicSequence analysisAdd BLAST62

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002147631 – 799Sodium- and chloride-dependent glycine transporter 2Add BLAST799

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineBy similarity1
Modified residuei59PhosphothreonineCombined sources1
Modified residuei86PhosphoserineCombined sources1
Disulfide bondi313 ↔ 322By similarity
Glycosylationi345N-linked (GlcNAc...)Sequence analysis1
Glycosylationi355N-linked (GlcNAc...)Sequence analysis1
Glycosylationi360N-linked (GlcNAc...)Sequence analysis1
Glycosylationi366N-linked (GlcNAc...)Sequence analysis1
Glycosylationi714N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ761V0.
PRIDEiQ761V0.

PTM databases

iPTMnetiQ761V0.
PhosphoSitePlusiQ761V0.

Expressioni

Tissue specificityi

Isoform a and isoform b are expressed at high levels in brain stem and spinal cord. Isoform a is also expressed in the cerebellum.1 Publication

Interactioni

Protein-protein interaction databases

IntActiQ761V0. 1 interactor.
MINTiMINT-4133901.
STRINGi10090.ENSMUSP00000058699.

Structurei

3D structure databases

ProteinModelPortaliQ761V0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiQ761V0.
PhylomeDBiQ761V0.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 2 hits.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a1 Publication (identifier: Q761V0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDCSAPKEMN KQPANILEAA VPGHRDSPRA PRTSPEQDLP AEAPAATVQP
60 70 80 90 100
PRVPRSASTG AQTFQSADAR ACEAQQSGVG FCNLSSPRAQ ATSAALRDLS
110 120 130 140 150
EGHSAQANPP SGPAGAGNAL HCKIPALRGP EEDANVSVGK GTLEHNNTPA
160 170 180 190 200
VGWVNMSQST VVLGTDGIAS VLPGSVATTT IPEDEQGDEN KARGNWSSKL
210 220 230 240 250
DFILSMVGYA VGLGNVWRFP YLAFQNGGGA FLIPYLMMLA LAGLPIFFLE
260 270 280 290 300
VSLGQFASQG PVSVWKAIPA LQGCGIAMLI ISVLIAIYYN VIICYTLFYL
310 320 330 340 350
FASFVSVLPW GSCNNPWNTP ECKDKTKLLL DSCVIGDHPK IQIKNSTFCM
360 370 380 390 400
TAYPNLTMVN FTSQTNKTFV SGSEEYFKYF VLKISAGIEY PGEIRWPLAF
410 420 430 440 450
CLFLAWVIVY ASLAKGIKSS GKVVYFTATF PYVVLVILLI RGVTLPGAGA
460 470 480 490 500
GIWYFITPKW EKLTDATVWK DAATQIFFSL SAAWGGLITL SSYNKFHNNC
510 520 530 540 550
YRDTLIVTCT NSATSIFAGF VIFSVIGFMA NERKVNIENV ADQGPGIAFV
560 570 580 590 600
VYPEALTRLP LSPFWAIIFF LMLLTLGLDT MFATIETIVT SISDEFPKYL
610 620 630 640 650
RTHKPVFTLG CCICFFIMGF PMITQGGIYM FQLVDTYAAS YALVIIAIFE
660 670 680 690 700
LVGISYVYGL QRFCEDIEMM IGFKPNIFWK VCWAFVTPTI LTFILCFSFY
710 720 730 740 750
QWEPMTYGSY RYPNWSMVLG WLMLACSVIW IPIMFVIKMY LAPGRFIERL
760 770 780 790
KLVCSPQPDW GPFLAQHRGE RYKNMIDPLG TSSLGLKLPV KDLELGTQC
Length:799
Mass (Da):87,861
Last modified:July 5, 2004 - v1
Checksum:i92AC7750C7023D45
GO
Isoform b1 Publication (identifier: Q761V0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Show »
Length:791
Mass (Da):86,999
Checksum:i5F7051201736EC01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti151V → E in AAL17054 (Ref. 2) Curated1
Sequence conflicti151V → E in AAN11408 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0516031 – 8Missing in isoform b. 2 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB118159 mRNA. Translation: BAD16781.1.
AF411042 mRNA. Translation: AAL17054.1.
AY147186 mRNA. Translation: AAN11408.1.
CCDSiCCDS21308.1. [Q761V0-2]
UniGeneiMm.207053.

Genome annotation databases

UCSCiuc012fle.1. mouse. [Q761V0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB118159 mRNA. Translation: BAD16781.1.
AF411042 mRNA. Translation: AAL17054.1.
AY147186 mRNA. Translation: AAN11408.1.
CCDSiCCDS21308.1. [Q761V0-2]
UniGeneiMm.207053.

3D structure databases

ProteinModelPortaliQ761V0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ761V0. 1 interactor.
MINTiMINT-4133901.
STRINGi10090.ENSMUSP00000058699.

Protein family/group databases

TCDBi2.A.22.2.6. the neurotransmitter:sodium symporter (nss) family.

PTM databases

iPTMnetiQ761V0.
PhosphoSitePlusiQ761V0.

Proteomic databases

PaxDbiQ761V0.
PRIDEiQ761V0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc012fle.1. mouse. [Q761V0-1]

Organism-specific databases

MGIiMGI:105090. Slc6a5.

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiQ761V0.
PhylomeDBiQ761V0.

Miscellaneous databases

PROiQ761V0.
SOURCEiSearch...

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 2 hits.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSC6A5_MOUSE
AccessioniPrimary (citable) accession number: Q761V0
Secondary accession number(s): Q8CFM5, Q91ZQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.