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Protein

Sodium- and chloride-dependent glycine transporter 2

Gene

Slc6a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi208Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi210Sodium 2; via carbonyl oxygenBy similarity1
Metal bindingi211Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi215Sodium 2By similarity1
Metal bindingi479Sodium 2By similarity1
Metal bindingi511Sodium 2By similarity1
Metal bindingi576Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi579Sodium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processNeurotransmitter transport, Symport, Transport
LigandMetal-binding, Sodium

Protein family/group databases

TCDBi2.A.22.2.6 the neurotransmitter:sodium symporter (nss) family

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent glycine transporter 2
Short name:
GlyT-2
Short name:
GlyT2
Alternative name(s):
Solute carrier family 6 member 5
Gene namesi
Name:Slc6a5Imported
Synonyms:Glyt2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:105090 Slc6a5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 201CytoplasmicSequence analysisAdd BLAST201
Transmembranei202 – 222Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei229 – 249Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei273 – 293Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini294 – 396ExtracellularSequence analysisAdd BLAST103
Transmembranei397 – 417Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei433 – 453Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei472 – 492Helical; Name=6Sequence analysisAdd BLAST21
Transmembranei510 – 530Helical; Name=7Sequence analysisAdd BLAST21
Transmembranei565 – 585Helical; Name=8Sequence analysisAdd BLAST21
Transmembranei611 – 631Helical; Name=9Sequence analysisAdd BLAST21
Transmembranei640 – 660Helical; Name=10Sequence analysisAdd BLAST21
Transmembranei677 – 697Helical; Name=11Sequence analysisAdd BLAST21
Transmembranei717 – 737Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini738 – 799CytoplasmicSequence analysisAdd BLAST62

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002147631 – 799Sodium- and chloride-dependent glycine transporter 2Add BLAST799

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineBy similarity1
Modified residuei59PhosphothreonineCombined sources1
Modified residuei86PhosphoserineCombined sources1
Disulfide bondi313 ↔ 322By similarity
Glycosylationi345N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi355N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi360N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi366N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi714N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ761V0
PRIDEiQ761V0

PTM databases

iPTMnetiQ761V0
PhosphoSitePlusiQ761V0

Expressioni

Tissue specificityi

Isoform a and isoform b are expressed at high levels in brain stem and spinal cord. Isoform a is also expressed in the cerebellum.1 Publication

Interactioni

Protein-protein interaction databases

IntActiQ761V0, 1 interactor
MINTiQ761V0
STRINGi10090.ENSMUSP00000058699

Structurei

3D structure databases

ProteinModelPortaliQ761V0
SMRiQ761V0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3659 Eukaryota
COG0733 LUCA
HOGENOMiHOG000116406
HOVERGENiHBG071421
InParanoidiQ761V0
PhylomeDBiQ761V0

Family and domain databases

InterProiView protein in InterPro
IPR000175 Na/ntran_symport
IPR037272 SNS_sf
PANTHERiPTHR11616 PTHR11616, 1 hit
PfamiView protein in Pfam
PF00209 SNF, 1 hit
PRINTSiPR00176 NANEUSMPORT
SUPFAMiSSF161070 SSF161070, 2 hits
PROSITEiView protein in PROSITE
PS00610 NA_NEUROTRAN_SYMP_1, 1 hit
PS00754 NA_NEUROTRAN_SYMP_2, 1 hit
PS50267 NA_NEUROTRAN_SYMP_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a1 Publication (identifier: Q761V0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDCSAPKEMN KQPANILEAA VPGHRDSPRA PRTSPEQDLP AEAPAATVQP
60 70 80 90 100
PRVPRSASTG AQTFQSADAR ACEAQQSGVG FCNLSSPRAQ ATSAALRDLS
110 120 130 140 150
EGHSAQANPP SGPAGAGNAL HCKIPALRGP EEDANVSVGK GTLEHNNTPA
160 170 180 190 200
VGWVNMSQST VVLGTDGIAS VLPGSVATTT IPEDEQGDEN KARGNWSSKL
210 220 230 240 250
DFILSMVGYA VGLGNVWRFP YLAFQNGGGA FLIPYLMMLA LAGLPIFFLE
260 270 280 290 300
VSLGQFASQG PVSVWKAIPA LQGCGIAMLI ISVLIAIYYN VIICYTLFYL
310 320 330 340 350
FASFVSVLPW GSCNNPWNTP ECKDKTKLLL DSCVIGDHPK IQIKNSTFCM
360 370 380 390 400
TAYPNLTMVN FTSQTNKTFV SGSEEYFKYF VLKISAGIEY PGEIRWPLAF
410 420 430 440 450
CLFLAWVIVY ASLAKGIKSS GKVVYFTATF PYVVLVILLI RGVTLPGAGA
460 470 480 490 500
GIWYFITPKW EKLTDATVWK DAATQIFFSL SAAWGGLITL SSYNKFHNNC
510 520 530 540 550
YRDTLIVTCT NSATSIFAGF VIFSVIGFMA NERKVNIENV ADQGPGIAFV
560 570 580 590 600
VYPEALTRLP LSPFWAIIFF LMLLTLGLDT MFATIETIVT SISDEFPKYL
610 620 630 640 650
RTHKPVFTLG CCICFFIMGF PMITQGGIYM FQLVDTYAAS YALVIIAIFE
660 670 680 690 700
LVGISYVYGL QRFCEDIEMM IGFKPNIFWK VCWAFVTPTI LTFILCFSFY
710 720 730 740 750
QWEPMTYGSY RYPNWSMVLG WLMLACSVIW IPIMFVIKMY LAPGRFIERL
760 770 780 790
KLVCSPQPDW GPFLAQHRGE RYKNMIDPLG TSSLGLKLPV KDLELGTQC
Length:799
Mass (Da):87,861
Last modified:July 5, 2004 - v1
Checksum:i92AC7750C7023D45
GO
Isoform b1 Publication (identifier: Q761V0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Show »
Length:791
Mass (Da):86,999
Checksum:i5F7051201736EC01
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti151V → E in AAL17054 (Ref. 2) Curated1
Sequence conflicti151V → E in AAN11408 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0516031 – 8Missing in isoform b. 2 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB118159 mRNA Translation: BAD16781.1
AF411042 mRNA Translation: AAL17054.1
AY147186 mRNA Translation: AAN11408.1
CCDSiCCDS21308.1 [Q761V0-2]
UniGeneiMm.207053

Genome annotation databases

UCSCiuc012fle.1 mouse [Q761V0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSC6A5_MOUSE
AccessioniPrimary (citable) accession number: Q761V0
Secondary accession number(s): Q8CFM5, Q91ZQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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