Reviewed, UniProtKB/Swiss-Prot Q75ZQ5 (URE1_HALMA)

Names and origin

Protein names

Recommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha

Gene names

Name:	ureC
Ordered Locus Names:	pNG7124

Encoded on

Plasmid pNG700 Ref.2

Organism

Haloarcula marismortui (Halobacterium marismortui) [Complete proteome] [HAMAP]

Taxonomic identifier

2238 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Haloarcula

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity.
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW urease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Urease subunit alpha HAMAP MF_01953		PRO_0000234193
Regions
	Domain	133 – 568	436	Urease
Sites
	Active site	320	1	Proton donor By similarity
	Metal binding	138	1	Nickel 2 By similarity
	Metal binding	140	1	Nickel 2 By similarity
	Metal binding	217	1	Nickel 1; via carbamate group By similarity
	Metal binding	217	1	Nickel 2; via carbamate group By similarity
	Metal binding	246	1	Nickel 1 By similarity
	Metal binding	272	1	Nickel 1 By similarity
	Metal binding	360	1	Nickel 2 By similarity
	Binding site	219	1	Substrate By similarity
Amino acid modifications
	Modified residue	217	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75ZQ5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: AA720B1E264B01CF	FASTA	568	60,622
	10 20 30 40 50 60 MTRDIDRENY AELYGPTTGD KVRLGDTELF AKVEEDLRTH GDEAVFGGGK TLRDGLGMAP 70 80 90 100 110 120 GVTQAEGALD WVLTNATIID PILGIVAADI GIRNGEIAGI GKAGNPDTMD GVDMVVGPST 130 140 150 160 170 180 DVYPAEGKIA TAGGLDIHIH FNSAQLHEHA LSGGITTMLG GGYGGGATTC TTGPENVKRF 190 200 210 220 230 240 LQAAEAWPVN VGFYGKGNAS DPGPLREQVE AGVCGLKLHE DWGSTPETIN TCLEVAEDED 250 260 270 280 290 300 VQVCMHTDTL NEAGFLENTF GAVDGRTMHL FHIEGAGGGH APDIMEMVGE PNMLPSSTNP 310 320 330 340 350 360 SMPYTDNTFD EHLDMVMVCH HLNPDVPEDV AFAESRVRAE TIAAEDVLHD MGAISMMTSD 370 380 390 400 410 420 SQAMGRIAEV IPRTWQTASK MKSQRGPLPE DEGTGADNHR IKRYIAKYTV NPAISAGIEE 430 440 450 460 470 480 HVGTLEPGKL ADICLWDPAF FGVKPAMTFK GGFPVHSEMG EANGSLMTCE PILQRERAGA 490 500 510 520 530 540 VGKAKHAISL SFVSPAAAEA GIDEEYGLDS RVVPIEGART PGKDDMVYNS YCPDDIEVDP 550 560 ETFEVRVDGE HVTCEPSSEL PLAQRYLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ureases of extreme halophiles of the genus Haloarcula with a unique structure of gene cluster." Mizuki T., Kamekura M., DasSarma S., Fukushima T., Usami R., Yoshida Y., Horikoshi K. Biosci. Biotechnol. Biochem. 68:397-406(2004) [PubMed: 14981304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966.
[2]	"Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea." Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W., Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E., Hood L., Ng W.V. Genome Res. 14:2221-2234(2004) [PubMed: 15520287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43049 / DSM 3752 / JCM 8966.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB119092 Genomic DNA. Translation: BAC84958.1. AY596296 Genomic DNA. Translation: AAV44835.1.
RefSeq	YP_134541.1.
3D structure databases
HSSP	HSSP built from PDB template 1A5N based on UniProtKB P18314.
ModBase	Search...
Protein family/group databases
MEROPS	M38.982.
Genome annotation databases
GeneID	3127158.
GenomeReviews	Gene locus pNG7124 in contig AY596296_GR.
KEGG	hma:pNG7124.
NMPDR	fig|272569.1.peg.3328.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q75ZQ5.
OMA	VNTFDEH.
Enzyme and pathway databases
BioCyc	HMAR272569:PNG7124-MON.
BRENDA	3.5.1.5. 88.
Family and domain databases
HAMAP	MF_01953. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011612. Urease_alpha_N. IPR005848. Urease_asu. IPR017951. Urease_asu_c. IPR017952. Urease_asu_core. IPR017950. Urease_asu_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
PRINTS	PR01752. UREASE.
TIGRFAMs	TIGR01792. urease_alph. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. 1 hit. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URE1_HALMA

Accession

Primary (citable) accession number: Q75ZQ5
Secondary accession number(s): Q5V6L7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)