Reviewed, UniProtKB/Swiss-Prot Q75ZP8 (P2OX_LYOSH)

Names and origin

Protein names

Recommended name:
    Pyranose 2-oxidase
      Short name=P2Ox
      Short name=Pyranose oxidase
      Short name=PROD
      Short name=POD
      Short name=POx
    EC=1.1.3.10
Alternative name(s):
    Pyranose:oxygen 2-oxidoreductase
    Glucose 2-oxidase
    FAD-oxidoreductase

Gene names

Name:	p2ox
Synonyms:	lsp2o

Organism

Lyophyllum shimeji (Hon-shimeji)

Taxonomic identifier

47721 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Tricholomataceae › Lyophyllum

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of O(2) to H(2)O(2) By similarity.
Catalytic activity	D-glucose + O(2) = 2-dehydro-D-glucose + H(2)O(2).
Cofactor	Binds 1 FAD covalently per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro pyranose oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	Pyranose 2-oxidase		PRO_0000205613
Sites
	Active site	540	1	By similarity
	Active site	583	1	By similarity
	Binding site	441	1	Substrate By similarity
	Binding site	443	1	Substrate By similarity
Amino acid modifications
	Modified residue	170	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75ZP8-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 255B99DF266BC541	FASTA	618	68,488
	10 20 30 40 50 60 MSLSTEQMLR DYPRSMQING QIPKNAIHET YGNDGVDVFI AGSGPIGATY AKLCVEAGLR 70 80 90 100 110 120 VVMVEIGAAD SFYAVNAEEG TAVPYVPGYH KKNEIEFQKD IDRFVNVIKG ALQQVSVPVR 130 140 150 160 170 180 NQNVPTLDPG AWSAPPGSSA ISNGKNPHQR EFENLSAEAV TRGVGGMSTH WTCSTPRIHP 190 200 210 220 230 240 PMESLPGIGR PKLSNDPAED DKEWNELYSE AERLIGTSTK EFDESIRHTL VLRSLQDAYK 250 260 270 280 290 300 DRQRIFRPLP LACHRLKNAP EYVEWHSAEN LFHSIYNDDK QKKLFTLLTN HRCTRLALTG 310 320 330 340 350 360 GYEKKIGAAE VRNLLATRNP SSQLDSYIMA KVYVLASGAI GNPQILYNSG FSGLQVTPRN 370 380 390 400 410 420 DSLIPNLGRY ITEQPMAFCQ IVLRQEFVDS VRDDPYGLPW WKEAVAQHIA KNPTDALPIP 430 440 450 460 470 480 FRDPEPQVTT PFTEEHPWHT QIHRDAFSYG AVGPEVDSRV IVDLRWFGAT DPEANNLLVF 490 500 510 520 530 540 QNDVQDGYSM PQPTFRYRPS TASNVRARKM MADMCEVASN LGGYLPTSPP QFMDPGLALH 550 560 570 580 590 600 LAGTTRIGFD KATTVADNNS LVWDFANLYV AGNGTIRTGF GENPTLTSMC HAIKSARSII 610 NTLKGGTDGK NTGEHRNL

References

[1]	"Identification and cloning of a novel pyranose oxidase with high catalytic activity from Lyophyllum shimeji and its expression in Escherichia coli." Takakura Y., Tsutsumi F., Inoue Y., Kuwata S. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB119106 mRNA. Translation: BAD12079.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR000172. GMC_OxRdtase_N. IPR012814. Pyranose_ox. [Graphical view]
Pfam	PF00732. GMC_oxred_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02462. pyranose_ox. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. False negative. PS00624. GMC_OXRED_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2OX_LYOSH

Accession

Primary (citable) accession number: Q75ZP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)