Q75Z89 (5HT2A_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 2A
Short name=5-HT-2
Short name=5-HT-2A

Alternative name(s):
Serotonin receptor 2A

Gene names

Name:	HTR2A
Synonyms:	5HTR2A

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production By similarity.
Subunit structure	Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Note: Localizes to the postsynaptic thickening of axo-dendritic synapses By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara behavior Inferred from electronic annotation. Source: InterPro phosphatidylinositol 3-kinase cascade Inferred from electronic annotation. Source: Compara phosphatidylinositol biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of diacylglycerol biosynthetic process Inferred from electronic annotation. Source: InterPro positive regulation of inositol trisphosphate biosynthetic process Inferred from electronic annotation. Source: InterPro regulation of behavior Inferred from electronic annotation. Source: InterPro regulation of blood vessel size Inferred from electronic annotation. Source: InterPro regulation of hormone secretion Inferred from electronic annotation. Source: InterPro release of sequestered calcium ion into cytosol Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara smooth muscle contraction Inferred from electronic annotation. Source: InterPro
Cellular_component	dendritic shaft Inferred from electronic annotation. Source: InterPro integral to plasma membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	1-(4-iodo-2,5-dimethoxyphenyl)propan-2-amine binding Inferred from electronic annotation. Source: Compara drug binding Inferred from electronic annotation. Source: Compara phosphatidylinositol phospholipase C activity Inferred from electronic annotation. Source: Compara serotonin binding Inferred from electronic annotation. Source: Compara serotonin receptor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	5-hydroxytryptamine receptor 2A		PRO_0000068942
Regions
	Topological domain	1 – 78	78	Extracellular By similarity
	Transmembrane	79 – 99	21	Helical; Name=1; By similarity
	Topological domain	100 – 115	16	Cytoplasmic By similarity
	Transmembrane	116 – 136	21	Helical; Name=2; By similarity
	Topological domain	137 – 148	12	Extracellular By similarity
	Transmembrane	149 – 169	21	Helical; Name=3; By similarity
	Topological domain	170 – 192	23	Cytoplasmic By similarity
	Transmembrane	193 – 213	21	Helical; Name=4; By similarity
	Topological domain	214 – 233	20	Extracellular By similarity
	Transmembrane	234 – 254	21	Helical; Name=5; By similarity
	Topological domain	255 – 322	68	Cytoplasmic By similarity
	Transmembrane	323 – 343	21	Helical; Name=6; By similarity
	Topological domain	344 – 358	15	Extracellular By similarity
	Transmembrane	359 – 379	21	Helical; Name=7; By similarity
	Topological domain	380 – 470	91	Cytoplasmic By similarity
	Motif	468 – 470	3	PDZ-binding
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Disulfide bond	148 ↔ 227		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q75Z89 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 85414E50CA9A91F0	FASTA	470	52,571
	10 20 30 40 50 60 MDILCEENTS LSSTTNSLMQ LHADTRLYST DFNSGEGNTS NAFNWTVDSE NRTNLSCEGC 70 80 90 100 110 120 LSPPCFSLLH LQEKNWSALL TAVVIILTIA GNILVIMAVS LEKKLQNATN YFLMSLAIAD 130 140 150 160 170 180 MLLGFLVMPV STLTILYGYR WPLPSKLCAV WIYLDVLFST ASIMHLCAIS LDRYVAIQNP 190 200 210 220 230 240 IHHSRFNSRT KAFLKIIAVW TISVGISMPI PVFGLQDDSK VFKEGSCLLA DENFVLIGSF 250 260 270 280 290 300 VAFFIPLTIM VITYFLTIKS LQKEATLCVS DPGTRTKLAS FSFLPQSSLS SEKLFQRSIH 310 320 330 340 350 360 REPGSYGRRT MQSISNEQKA CKVLGIVFFL FVVMWCPFFI TNIMAVICKE SCNRDVIEAL 370 380 390 400 410 420 LNVFVWIGYL SSAVNPLVYT LFNKTYRSAF SRYIQCQYKE NKKPLQLILV NTIPALAYKS 430 440 450 460 470 SQLQMGPKKN SKKDDKTTDN DCTMVALGKE HPEDAPADSS NTVNEKVSCV

References

[1]	"Expression of 5-HT2A during adipogenesis." Tahara K., Aso H., Yamasaki T., Yamaguchi T., Takano S. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adipose tissue.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB119637 mRNA. Translation: BAD12238.1.
IPI	IPI00688690.
RefSeq	NP_001001157.1. NM_001001157.1.
UniGene	Bt.27990.
3D structure databases
ProteinModelPortal	Q75Z89.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000017943.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	Q75Z89.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000017943; ENSBTAP00000017943; ENSBTAG00000013498.
GeneID	407230.
KEGG	bta:407230.
Organism-specific databases
CTD	3356.
Phylogenomic databases
eggNOG	NOG247243.
GeneTree	ENSGT00700000104081.
HOGENOM	HOG000240378.
HOVERGEN	HBG107487.
InParanoid	Q75Z89.
KO	K04157.
OMA	FITNVMA.
OrthoDB	EOG4TMR24.
Enzyme and pathway databases
BioCyc	CATTLE:407230-MONOMER.
Gene expression databases
ArrayExpress	Q75Z89.
Family and domain databases
InterPro	IPR000455. 5HT2A_rcpt. IPR002231. 5HT_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
PANTHER	PTHR24247. PTHR24247. 1 hit. PTHR24247:SF30. PTHR24247:SF30. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00516. 5HT2ARECEPTR. PR01101. 5HTRECEPTOR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q75Z89.
ChEMBL	CHEMBL3446.
NextBio	20818480.

Entry information

Entry name

5HT2A_BOVIN

Accession

Primary (citable) accession number: Q75Z89

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program