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Protein

Probable trehalose-phosphate phosphatase 1

Gene

TPP1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Removes the phosphate from trehalose 6-phosphate to produce free trehalose. Trehalose accumulation in plant improves abiotic stress tolerance.3 Publications

Catalytic activityi

Trehalose 6-phosphate + H2O = trehalose + phosphate.2 Publications

Cofactori

Kineticsi

  1. KM=92.1 µM for trehalose 6-phosphate1 Publication

    pH dependencei

    Optimum pH is 6.5-7.0.1 Publication

    Pathwayi: trehalose biosynthesis

    This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

    GO - Molecular functioni

    • trehalose-phosphatase activity Source: UniProtKB

    GO - Biological processi

    • dephosphorylation Source: GOC
    • response to cold Source: UniProtKB
    • response to salt stress Source: UniProtKB
    • trehalose biosynthetic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    BRENDAi3.1.3.12. 4460.
    UniPathwayiUPA00299.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable trehalose-phosphate phosphatase 1 (EC:3.1.3.12)
    Short name:
    OsTPP1
    Alternative name(s):
    Trehalose 6-phosphate phosphatase
    Gene namesi
    Name:TPP1
    Ordered Locus Names:Os02g0661100, LOC_Os02g44230
    ORF Names:OsJ_07820, P0516F12.9, P0708H12.35
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
    Proteomesi
    • UP000059680 Componenti: Chromosome 2

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 371371Probable trehalose-phosphate phosphatase 1PRO_0000417653Add
    BLAST

    Proteomic databases

    PaxDbiQ75WV3.

    Expressioni

    Tissue specificityi

    Expressed in roots and shoots.1 Publication

    Inductioni

    By cold, drought, salt stress and abscisic acid (ABA).3 Publications

    Gene expression databases

    ExpressionAtlasiQ75WV3. baseline and differential.
    GenevisibleiQ75WV3. OS.

    Interactioni

    Protein-protein interaction databases

    STRINGi39947.LOC_Os02g44230.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ75WV3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the trehalose phosphatase family.Curated

    Phylogenomic databases

    eggNOGiENOG410IMM9. Eukaryota.
    COG1877. LUCA.
    InParanoidiQ75WV3.
    KOiK01087.
    OMAiGMDIMAS.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR003337. Trehalose_PPase.
    [Graphical view]
    PfamiPF02358. Trehalose_PPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
    TIGR00685. T6PP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q75WV3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDLSNSSPVI TDPVAISQQL LGGLPSNLMQ FSVMPGGYSS SGMNVGVSRL
    60 70 80 90 100
    KIEEVLVNGL LDAMKSSSPR RRLNVAFGED NSSEEEDPAY SAWMAKCPSA
    110 120 130 140 150
    LASFKQIVAS AQGKKIAVFL DYDGTLSPIV DDPDKAVMSP VMRAAVRNVA
    160 170 180 190 200
    KYFPTAIVSG RSRNKVFEFV KLKELYYAGS HGMDIMAPSA NHEHSAEKSK
    210 220 230 240 250
    QANLFQPAHD FLPMIDEVTK SLLQVVSGIE GATVENNKFC VSVHYRNVAE
    260 270 280 290 300
    KDWKLVARLV NEVLEAFPRL KVTNGRMVLE VRPVIDWDKG KAVEFLLQSL
    310 320 330 340 350
    GLNDSENVIP IYIGDDRTDE DAFKVLRQRN CGYGILVSQV PKETEAFYSL
    360 370
    RDPSEVMEFL NFLVRWKKHS V
    Length:371
    Mass (Da):41,128
    Last modified:July 5, 2004 - v1
    Checksum:i9F518916127CCD34
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB120515 mRNA. Translation: BAD12596.1.
    AP004883 Genomic DNA. Translation: BAD25622.1.
    AP005072 Genomic DNA. Translation: BAD25753.1.
    AP008208 Genomic DNA. Translation: BAF09559.1.
    AP014958 Genomic DNA. Translation: BAS80139.1.
    CM000139 Genomic DNA. Translation: EEE57517.1.
    AK103391 mRNA. Translation: BAG96056.1.
    RefSeqiXP_015623255.1. XM_015767769.1.
    UniGeneiOs.6092.

    Genome annotation databases

    EnsemblPlantsiOS02T0661100-01; OS02T0661100-01; OS02G0661100.
    GeneIDi4330221.
    GrameneiOS02T0661100-01; OS02T0661100-01; OS02G0661100.
    KEGGiosa:4330221.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB120515 mRNA. Translation: BAD12596.1.
    AP004883 Genomic DNA. Translation: BAD25622.1.
    AP005072 Genomic DNA. Translation: BAD25753.1.
    AP008208 Genomic DNA. Translation: BAF09559.1.
    AP014958 Genomic DNA. Translation: BAS80139.1.
    CM000139 Genomic DNA. Translation: EEE57517.1.
    AK103391 mRNA. Translation: BAG96056.1.
    RefSeqiXP_015623255.1. XM_015767769.1.
    UniGeneiOs.6092.

    3D structure databases

    ProteinModelPortaliQ75WV3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi39947.LOC_Os02g44230.1.

    Proteomic databases

    PaxDbiQ75WV3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiOS02T0661100-01; OS02T0661100-01; OS02G0661100.
    GeneIDi4330221.
    GrameneiOS02T0661100-01; OS02T0661100-01; OS02G0661100.
    KEGGiosa:4330221.

    Phylogenomic databases

    eggNOGiENOG410IMM9. Eukaryota.
    COG1877. LUCA.
    InParanoidiQ75WV3.
    KOiK01087.
    OMAiGMDIMAS.

    Enzyme and pathway databases

    UniPathwayiUPA00299.
    BRENDAi3.1.3.12. 4460.

    Gene expression databases

    ExpressionAtlasiQ75WV3. baseline and differential.
    GenevisibleiQ75WV3. OS.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR003337. Trehalose_PPase.
    [Graphical view]
    PfamiPF02358. Trehalose_PPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
    TIGR00685. T6PP. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Functional identification of a trehalose 6-phosphate phosphatase gene that is involved in transient induction of trehalose biosynthesis during chilling stress in rice."
      Pramanik M.H., Imai R.
      Plant Mol. Biol. 58:751-762(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, INDUCTION, GENE FAMILY, NOMENCLATURE.
      Strain: cv. Yukihikari.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    5. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    6. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.
    7. "Biochemical characterization of rice trehalose-6-phosphate phosphatases supports distinctive functions of these plant enzymes."
      Shima S., Matsui H., Tahara S., Imai R.
      FEBS J. 274:1192-1201(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
    8. "Overexpression of the trehalose-6-phosphate phosphatase gene OsTPP1 confers stress tolerance in rice and results in the activation of stress responsive genes."
      Ge L.F., Chao D.Y., Shi M., Zhu M.Z., Gao J.P., Lin H.X.
      Planta 228:191-201(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiTPP1_ORYSJ
    AccessioniPrimary (citable) accession number: Q75WV3
    Secondary accession number(s): A0A0P0VMN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2012
    Last sequence update: July 5, 2004
    Last modified: June 8, 2016
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Over-expression of TPP1 increases trehalose levels and enhances tolerance to salt and cold stresses.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.