Q75WM6 (H1FNT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Testis-specific H1 histone

Alternative name(s):
Haploid germ cell-specific nuclear protein 1
Histone H1t2

Gene names

Name:	H1FNT
Synonyms:	HANP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential for normal spermatogenesis and male fertility. Required for proper cell restructuring and DNA condensation during the elongation phase of spermiogenesis. Involved in the histone-protamine transition of sperm chromatin and the subsequent production of functional sperm. Binds both double-stranded and single-stranded DNA, ATP and protamine-1 By similarity. Ref.4
Subcellular location	Nucleus By similarity. Chromosome By similarity. Note: In round and elongating spermatids, specifically localizes to a chromatin domain at the apical pole By similarity.
Tissue specificity	Testis-specific. Ref.4
Sequence similarities	Belongs to the histone H1/H5 family.
Caution	It is uncertain whether Met-1 or Met-22 is the initiator.
Sequence caution	The sequence BAD13382.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA condensation Differentiation Spermatogenesis
Cellular component	Chromosome Nucleus
Coding sequence diversity	Polymorphism
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromosome condensation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW sperm chromatin condensation Inferred from sequence or structural similarity Ref.5. Source: UniProtKB spermatid nucleus elongation Inferred from sequence or structural similarity Ref.5. Source: UniProtKB
Cellular component	nuclear chromatin Inferred from sequence or structural similarity Ref.5. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Testis-specific H1 histone		PRO_0000343414
Regions
	Compositional bias	129 – 195	67	Arg-rich
Natural variations
	Natural variant	84	1	R → G in a Japanese man. Ref.4 Corresponds to variant rs2732441 [ dbSNP | Ensembl ].		VAR_044371
	Natural variant	108	1	L → P in a Japanese man. Ref.4		VAR_044372
	Natural variant	136	1	R → S in a Japanese man. Ref.4		VAR_044373
	Natural variant	174	1	R → Q in a Japanese man. Ref.3 Ref.4 Corresponds to variant rs1471997 [ dbSNP | Ensembl ].		VAR_044374
	Natural variant	195	1	R → Q. Corresponds to variant rs1471997 [ dbSNP | Ensembl ].		VAR_047359
	Natural variant	237	1	S → F in a Japanese man. Ref.4 Corresponds to variant rs2291483 [ dbSNP | Ensembl ].		VAR_044375
Experimental info
	Sequence conflict	231	1	K → N in AAI18636. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q75WM6 [UniParc]. Last modified November 25, 2008. Version 3. Checksum: F5BA8AB6A4D4E912	FASTA	255	28,116
	10 20 30 40 50 60 MEQALTGEAQ SRWPRRGGSG AMAEAPGPSG ESRGHSATQL PAEKTVGGPS RGCSSSVLRV 70 80 90 100 110 120 SQLVLQAIST HKGLTLAALK KELRNAGYEV RRKSGRHEAP RGQAKATLLR VSGSDAAGYF 130 140 150 160 170 180 RVWKVPKPRR KPGRARQEEG TRAPWRTPAA PRSSRRRRQP LRKAARKARE VWRRNARAKA 190 200 210 220 230 240 KANARARRTR RARPRAKEPP CARAKEEAGA TAADEGRGQA VKEDTTPRSG KDKRRSSKPR 250 EEKQEPKKPA QRTIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Huang C.Q., Shan Y.X., Wu S.L., Cheng Z. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed: 16541075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLN-174.
[4]	"Expression profiles and single-nucleotide polymorphism analysis of human HANP1/H1T2 encoding a histone H1-like protein." Tanaka H., Matsuoka Y., Onishi M., Kitamura K., Miyagawa Y., Nishimura H., Tsujimura A., Okuyama A., Nishimune Y. Int. J. Androl. 29:353-359(2006) [PubMed: 16533358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-255, FUNCTION, TISSUE SPECIFICITY, VARIANTS GLY-84; PRO-108; SER-136; GLN-174 AND PHE-237. Tissue: Testis.
[5]	"Polar nuclear localization of H1T2, a histone H1 variant, required for spermatid elongation and DNA condensation during spermiogenesis." Martianov I., Brancorsini S., Catena R., Gansmuller A., Kotaja N., Parvinen M., Sassone-Corsi P., Davidson I. Proc. Natl. Acad. Sci. U.S.A. 102:2808-2813(2005) [PubMed: 15710904] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-255.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY302593 mRNA. Translation: AAP60022.1. AC024257 Genomic DNA. No translation available. BC118635 mRNA. Translation: AAI18636.1. BC119787 mRNA. Translation: AAI19788.1. AB121028 mRNA. Translation: BAD13382.2. Different initiation. AY496854 Genomic DNA. Translation: AAS49492.1.
IPI	IPI00376239.
RefSeq	NP_861453.1. NM_181788.1.
UniGene	Hs.155833.
3D structure databases
ProteinModelPortal	Q75WM6.
SMR	Q75WM6. Positions 59-124.
ModBase	Search...
Protein-protein interaction databases
STRING	Q75WM6.
Polymorphism databases
DMDM	215274115.
Proteomic databases
PRIDE	Q75WM6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000335017; ENSP00000334805; ENSG00000187166.
GeneID	341567.
KEGG	hsa:341567.
UCSC	uc001rrm.1. human.
Organism-specific databases
CTD	341567.
GeneCards	GC12P048722.
H-InvDB	HIX0036674.
HGNC	HGNC:24893. H1FNT.
neXtProt	NX_Q75WM6.
PharmGKB	PA143485486.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17471.
GeneTree	ENSGT00390000012442.
InParanoid	Q75WM6.
OMA	RKAREVW.
OrthoDB	EOG49CQ91.
PhylomeDB	Q75WM6.
Gene expression databases
ArrayExpress	Q75WM6.
Bgee	Q75WM6.
CleanEx	HS_H1FNT.
Genevestigator	Q75WM6.
Family and domain databases
ProtoNet	Search...
Other
NextBio	98168.

Entry information

Entry name

H1FNT_HUMAN

Accession

Primary (citable) accession number: Q75WM6
Secondary accession number(s): Q147U8, Q5GKZ5, Q7Z694

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	November 25, 2008
Last modified:	January 25, 2012
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.