Aminotransferase - Thermus thermophilus

Preferred order of sections

Names and origin

Protein names	Submitted name: Aminotransferase EMBL BAD06364.1
Organism	Thermus thermophilus EMBL BAD06364.1
Taxonomic identifier	274 [NCBI]
Taxonomic lineage	Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Pyridoxal phosphate PDB 1V2F PDB 1V2E PDB 1V2D
Molecular function	Aminotransferase EMBL BAD06364.1 Transferase
Technical term	3D-structure PDB 1V2F PDB 1V2E PDB 1V2D
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Binding site

33

1

3-phenylpropanoic acid; via amide nitrogen PDB 1V2F

Binding site

163

1

3-phenylpropanoic acid PDB 1V2F

Binding site

253

1

3-phenylpropanoic acid PDB 1V2F

Binding site

347

1

3-phenylpropanoic acid PDB 1V2F

Amino acid modifications

Modified residue

222

1

Pyridoxal phosphate PDB 1V2F PDB 1V2E PDB 1V2D

Sequences

Sequence

Length

Mass (Da)

Tools

Q75WK2 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C5AD55C24D45E2EF
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FASTA

381

42,271

        10         20         30         40         50         60 
MRLHPRTEAA KESIFPRMSG LAQRLGAVNL GQGFPSNPPP PFLLEAVRRA LGRQDQYAPP 

        70         80         90        100        110        120 
AGLPALREAL AEEFAVEPES VVVTSGATEA LYVLLQSLVG PGDEVVVLEP FFDVYLPDAF 

       130        140        150        160        170        180 
LAGAKARLVR LDLTPEGFRL DLSALEKALT PRTRALLLNT PMNPTGLVFG ERELEAIARL 

       190        200        210        220        230        240 
ARAHDLFLIS DEVYDELYYG ERPRRLREFA PERTFTVGSA GKRLEATGYR VGWIVGPKEF 

       250        260        270        280        290        300 
MPRLAGMRQW TSFSAPTPLQ AGVAEALKLA RREGFYEALR EGYRRRRDLL AGGLRAMGLR 

       310        320        330        340        350        360 
VYVPEGTYFL MAELPGWDAF RLVEEARVAL IPASAFYLED PPKDLFRFAF CKTEEELHLA 

       370        380 
LERLGRVVNS PREAEGGAVS G

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References

[1]	"Glutamine:phenylpyruvate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8." Hosono A., Mizuguchi H., Hayashi H., Goto M., Miyahara I., Hirotsu K., Kagamiyama H. J. Biochem. 134:843-851(2003) [PubMed: 14769873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: HB8 EMBL BAD06364.1.
[2]	Masui R., Inoue Y., Shibata T., Miki K., Yokoyama S., Kuramitsu S. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: HB8 EMBL BAD06364.1.
[3]	"Crystal structures of glutamine:phenylpyruvate aminotransferase from Thermus thermophilus HB8: induced fit and substrate recognition." Goto M., Omi R., Miyahara I., Hosono A., Mizuguchi H., Hayashi H., Kagamiyama H., Hirotsu K. J. Biol. Chem. 279:16518-16525(2004) [PubMed: 14761974] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH 3-PHENYLPROPANOIC ACID, PYRIDOXAL PHOSPHATE AT LYS-222.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB121092 Genomic DNA. Translation: BAD06364.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V2D	X-ray	1.90	A	1-381	[»]
1V2E	X-ray	2.60	A/B	1-381	[»]
1V2F	X-ray	2.35	A/B	1-381	[»]

ProteinModelPortal

Q75WK2.

SMR

Q75WK2. Positions 1-368.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]

Gene3D

G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.

Pfam

PF00155. Aminotran_1_2. 1 hit.
[Graphical view]

SUPFAM

SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q75WK2_THETH

Accession

Primary (citable) accession number: Q75WK2

Entry history

Integrated into UniProtKB/TrEMBL:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	July 27, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)