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Q75WH8

- Q75WH8_PSEST

UniProt

Q75WH8 - Q75WH8_PSEST

Protein
Submitted name:

L-rhamnose isomerase

Gene

L-RhI

Organism
Pseudomonas stutzeri (Pseudomonas perfectomarina)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi219 – 2191Cobalt 1
    Metal bindingi219 – 2191Copper 1Imported
    Metal bindingi219 – 2191Manganese 1Imported
    Metal bindingi219 – 2191Zinc 1Imported
    Metal bindingi254 – 2541Cobalt 1
    Metal bindingi254 – 2541Copper 1Imported
    Metal bindingi254 – 2541Manganese 1Imported
    Metal bindingi254 – 2541Zinc 1Imported
    Metal bindingi257 – 2571Cobalt 2; via tele nitrogen
    Metal bindingi257 – 2571Copper 2; via tele nitrogenImported
    Metal bindingi257 – 2571Copper 3; via tele nitrogenImported
    Metal bindingi257 – 2571Manganese 2; via tele nitrogenImported
    Metal bindingi257 – 2571Manganese 3; via tele nitrogenImported
    Metal bindingi257 – 2571Zinc 2; via tele nitrogenImported
    Metal bindingi257 – 2571Zinc 3; via tele nitrogen
    Metal bindingi281 – 2811Cobalt 1; via pros nitrogen
    Metal bindingi281 – 2811Copper 1; via pros nitrogenImported
    Metal bindingi281 – 2811Manganese 1; via pros nitrogenImported
    Metal bindingi281 – 2811Zinc 1; via pros nitrogenImported
    Metal bindingi289 – 2891Cobalt 2
    Metal bindingi289 – 2891Copper 2Imported
    Metal bindingi289 – 2891Copper 3Imported
    Metal bindingi289 – 2891Manganese 2Imported
    Metal bindingi289 – 2891Manganese 3Imported
    Metal bindingi289 – 2891Zinc 2Imported
    Metal bindingi289 – 2891Zinc 3
    Metal bindingi291 – 2911Cobalt 2
    Metal bindingi291 – 2911Copper 2Imported
    Metal bindingi291 – 2911Copper 3Imported
    Metal bindingi291 – 2911Manganese 2Imported
    Metal bindingi291 – 2911Manganese 3Imported
    Metal bindingi291 – 2911Zinc 2Imported
    Metal bindingi291 – 2911Zinc 3
    Metal bindingi298 – 2981Manganese 1Imported
    Metal bindingi327 – 3271Cobalt 1
    Metal bindingi327 – 3271Copper 1Imported
    Metal bindingi327 – 3271Manganese 1Imported
    Metal bindingi327 – 3271Zinc 1Imported

    GO - Molecular functioni

    1. isomerase activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    IsomeraseImported

    Keywords - Ligandi

    CopperImported, ManganeseImported, Metal-bindingImported, ZincImported

    Enzyme and pathway databases

    BRENDAi5.3.1.14. 421.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    L-rhamnose isomeraseImported
    Gene namesi
    Name:L-RhIImported
    OrganismiPseudomonas stutzeri (Pseudomonas perfectomarina)Imported
    Taxonomic identifieri316 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-7384859.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HCVX-ray2.00A/B/C/D1-430[»]
    2I56X-ray1.97A/B/C/D1-430[»]
    2I57X-ray1.97A/B/C/D1-430[»]
    3ITLX-ray1.70A/B/C/D1-430[»]
    3ITOX-ray1.90A/B/C/D1-430[»]
    3ITTX-ray1.96A/B/C/D1-430[»]
    3ITVX-ray1.60A/B/C/D1-430[»]
    3ITXX-ray1.80A/B/C/D1-430[»]
    3ITYX-ray1.84A/B/C/D1-430[»]
    3IUDX-ray2.44A/B/C/D1-430[»]
    3IUHX-ray2.00A/B/C/D1-430[»]
    3IUIX-ray2.30A/B/C/D1-430[»]
    3M0HX-ray1.58A/B/C/D1-430[»]
    3M0LX-ray1.85A/B/C/D1-430[»]
    3M0MX-ray1.45A/B/C/D1-430[»]
    3M0VX-ray1.79A/B/C/D1-430[»]
    3M0XX-ray1.79A/B/C/D1-430[»]
    3M0YX-ray1.96A/B/C/D1-430[»]
    4GJIX-ray1.70A/B/C/D1-430[»]
    4GJJX-ray2.38A/B/C/D1-430[»]
    ProteinModelPortaliQ75WH8.
    SMRiQ75WH8. Positions 3-430.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ75WH8.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.20.20.150. 1 hit.
    InterProiIPR013451. L_rhamnose_iso.
    IPR013022. Xyl_isomerase-like_TIM-brl.
    [Graphical view]
    PfamiPF01261. AP_endonuc_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF51658. SSF51658. 1 hit.
    TIGRFAMsiTIGR02629. L_rham_iso_rhiz. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q75WH8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEFRIAQDV VARENDRRAS ALKEDYEALG ANLARRGVDI EAVTAKVEKF    50
    FVAVPSWGVG TGGTRFARFP GTGEPRGIFD KLDDCAVIQQ LTRATPNVSL 100
    HIPWDKADPK ELKARGDALG LGFDAMNSNT FSDAPGQAHS YKYGSLSHTD 150
    AATRAQAVEH NLECIEIGKA IGSKALTVWI GDGSNFPGQS NFTRAFERYL 200
    SAMAEIYKGL PDDWKLFSEH KMYEPAFYST VVQDWGTNYL IAQTLGPKAQ 250
    CLVDLGHHAP NTNIEMIVAR LIQFGKLGGF HFNDSKYGDD DLDAGAIEPY 300
    RLFLVFNELV DAEARGVKGF HPAHMIDQSH NVTDPIESLI NSANEIRRAY 350
    AQALLVDRAA LSGYQEDNDA LMATETLKRA YRTDVEPILA EARRRTGGAV 400
    DPVATYRASG YRARVAAERP ASVAGGGGII 430
    Length:430
    Mass (Da):46,975
    Last modified:July 5, 2004 - v1
    Checksum:i2E2FBA87068472A7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB121136 Genomic DNA. Translation: BAD14073.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB121136 Genomic DNA. Translation: BAD14073.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HCV X-ray 2.00 A/B/C/D 1-430 [» ]
    2I56 X-ray 1.97 A/B/C/D 1-430 [» ]
    2I57 X-ray 1.97 A/B/C/D 1-430 [» ]
    3ITL X-ray 1.70 A/B/C/D 1-430 [» ]
    3ITO X-ray 1.90 A/B/C/D 1-430 [» ]
    3ITT X-ray 1.96 A/B/C/D 1-430 [» ]
    3ITV X-ray 1.60 A/B/C/D 1-430 [» ]
    3ITX X-ray 1.80 A/B/C/D 1-430 [» ]
    3ITY X-ray 1.84 A/B/C/D 1-430 [» ]
    3IUD X-ray 2.44 A/B/C/D 1-430 [» ]
    3IUH X-ray 2.00 A/B/C/D 1-430 [» ]
    3IUI X-ray 2.30 A/B/C/D 1-430 [» ]
    3M0H X-ray 1.58 A/B/C/D 1-430 [» ]
    3M0L X-ray 1.85 A/B/C/D 1-430 [» ]
    3M0M X-ray 1.45 A/B/C/D 1-430 [» ]
    3M0V X-ray 1.79 A/B/C/D 1-430 [» ]
    3M0X X-ray 1.79 A/B/C/D 1-430 [» ]
    3M0Y X-ray 1.96 A/B/C/D 1-430 [» ]
    4GJI X-ray 1.70 A/B/C/D 1-430 [» ]
    4GJJ X-ray 2.38 A/B/C/D 1-430 [» ]
    ProteinModelPortali Q75WH8.
    SMRi Q75WH8. Positions 3-430.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-7384859.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 5.3.1.14. 421.

    Miscellaneous databases

    EvolutionaryTracei Q75WH8.

    Family and domain databases

    Gene3Di 3.20.20.150. 1 hit.
    InterProi IPR013451. L_rhamnose_iso.
    IPR013022. Xyl_isomerase-like_TIM-brl.
    [Graphical view ]
    Pfami PF01261. AP_endonuc_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51658. SSF51658. 1 hit.
    TIGRFAMsi TIGR02629. L_rham_iso_rhiz. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, nucleotide sequence, and overexpression of the L-rhamnose isomerase gene from Pseudomonas stutzeri in Escherichia coli."
      Leang K., Takada G., Ishimura A., Okita M., Izumori K.
      Appl. Environ. Microbiol. 70:3298-3304(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "The structures of L-rhamnose isomerase from Pseudomonas stutzeri in complexes with L-rhamnose and D-allose provide insights into broad substrate specificity."
      Yoshida H., Yamada M., Ohyama Y., Takada G., Izumori K., Kamitori S.
      J. Mol. Biol. 365:1505-1516(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.97 ANGSTROMS) IN COMPLEX WITH ZINC.
    3. "Catalytic reaction mechanism of Pseudomonas stutzeri L-rhamnose isomerase deduced from X-ray structures."
      Yoshida H., Yamaji M., Ishii T., Izumori K., Kamitori S.
      FEBS J. 277:1045-1057(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH COPPER; MANGANESE AND ZINC.
    4. "Elucidation of the role of Ser329 and the C-terminal region in the catalytic activity of Pseudomonas stutzeri L-rhamnose isomerase."
      Yoshida H., Takeda K., Izumori K., Kamitori S.
      Protein Eng. Des. Sel. 23:919-927(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) IN COMPLEX WITH MANGANESE.
    5. "Structure of l-rhamnose isomerase in complex with l-rhamnopyranose demonstrates the sugar-ring opening mechanism and the role of a substrate sub-binding site."
      Yoshida H., Yoshihara A., Teraoka M., Yamashita S., Izumori K., Kamitori S.
      FEBS Open Bio 3:35-40(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH MANGANESE.

    Entry informationi

    Entry nameiQ75WH8_PSEST
    AccessioniPrimary (citable) accession number: Q75WH8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 5, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3