Q75WH8 (Q75WH8_PSEST) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
L-rhamnose isomerase EMBL BAD14073.1

Gene names

Name:

L-RhI EMBL BAD14073.1

Organism

Pseudomonas stutzeri (Pseudomonas perfectomarina) EMBL BAD14073.1

Taxonomic identifier

316 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas stutzeri group › Pseudomonas stutzeri subgroup

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Cobalt PDB 3IUH Copper PDB 3IUD Manganese PDB 3ITL PDB 3ITX PDB 3ITV PDB 3ITT PDB 3ITO PDB 3M0H PDB 3M0L PDB 3M0M PDB 3M0V PDB 3M0Y PDB 3M0X Metal-binding PDB 2HCV PDB 2I56 PDB 2I57 PDB 3IUI PDB 3IUD PDB 3ITL PDB 3ITX PDB 3ITV PDB 3IUH PDB 3ITT PDB 3ITO PDB 3M0H PDB 3M0L PDB 3M0M PDB 3M0V PDB 3M0Y PDB 3M0X Zinc PDB 2HCV PDB 2I56 PDB 2I57 PDB 3IUI
Molecular function	Isomerase EMBL BAD14073.1
Technical term	3D-structure PDB 2HCV PDB 2I56 PDB 2I57 PDB 3IUI PDB 3IUD PDB 3ITL PDB 3ITY PDB 3ITX PDB 3ITV PDB 3IUH PDB 3ITT PDB 3ITO PDB 3M0H PDB 3M0L PDB 3M0M PDB 3M0V PDB 3M0Y PDB 3M0X PDB 4GJI PDB 4GJJ
Gene Ontology (GO)
Molecular_function	isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	219	1	Cobalt 1
	Metal binding	219	1	Copper 1 PDB 3IUD
	Metal binding	219	1	Manganese 1 PDB 3ITL PDB 3ITX PDB 3ITV PDB 3ITT PDB 3ITO PDB 3M0H PDB 3M0L PDB 3M0M PDB 3M0V PDB 3M0Y PDB 3M0X
	Metal binding	219	1	Zinc 1 PDB 2HCV PDB 2I56 PDB 2I57 PDB 3IUI
	Metal binding	254	1	Cobalt 1
	Metal binding	254	1	Copper 1 PDB 3IUD
	Metal binding	254	1	Manganese 1 PDB 3ITL PDB 3ITX PDB 3ITV PDB 3ITT PDB 3ITO PDB 3M0H PDB 3M0L PDB 3M0M PDB 3M0V PDB 3M0Y PDB 3M0X
	Metal binding	254	1	Zinc 1 PDB 2HCV PDB 2I56 PDB 2I57 PDB 3IUI
	Metal binding	257	1	Cobalt 2; via tele nitrogen
	Metal binding	257	1	Copper 2; via tele nitrogen PDB 3IUD
	Metal binding	257	1	Copper 3; via tele nitrogen PDB 3IUD
	Metal binding	257	1	Manganese 2; via tele nitrogen PDB 3ITT
	Metal binding	257	1	Manganese 3; via tele nitrogen PDB 3ITX
	Metal binding	257	1	Zinc 2; via tele nitrogen PDB 2I56 PDB 2I57 PDB 3IUI
	Metal binding	257	1	Zinc 3; via tele nitrogen PDB 2I56
	Metal binding	281	1	Cobalt 1; via pros nitrogen
	Metal binding	281	1	Copper 1; via pros nitrogen PDB 3IUD
	Metal binding	281	1	Manganese 1; via pros nitrogen PDB 3ITL PDB 3ITX PDB 3ITV PDB 3ITT PDB 3ITO PDB 3M0H PDB 3M0L PDB 3M0M PDB 3M0V PDB 3M0Y PDB 3M0X
	Metal binding	281	1	Zinc 1; via pros nitrogen PDB 2HCV PDB 2I56 PDB 2I57 PDB 3IUI
	Metal binding	289	1	Cobalt 2
	Metal binding	289	1	Copper 2 PDB 3IUD
	Metal binding	289	1	Copper 3 PDB 3IUD
	Metal binding	289	1	Manganese 2 PDB 3ITT
	Metal binding	289	1	Manganese 3 PDB 3ITX
	Metal binding	289	1	Zinc 2 PDB 2I56 PDB 2I57 PDB 3IUI
	Metal binding	289	1	Zinc 3 PDB 2I56
	Metal binding	291	1	Cobalt 2
	Metal binding	291	1	Copper 2 PDB 3IUD
	Metal binding	291	1	Copper 3 PDB 3IUD
	Metal binding	291	1	Manganese 3 PDB 3ITX
	Metal binding	291	1	Zinc 2 PDB 3IUI
	Metal binding	291	1	Zinc 3 PDB 2I56
	Metal binding	327	1	Cobalt 1
	Metal binding	327	1	Copper 1 PDB 3IUD
	Metal binding	327	1	Manganese 1 PDB 3ITX PDB 3ITV PDB 3ITT PDB 3M0H PDB 3M0L PDB 3M0M PDB 3M0V PDB 3M0Y PDB 3M0X
	Metal binding	327	1	Zinc 1 PDB 2HCV PDB 2I56 PDB 2I57 PDB 3IUI

Sequences

	Sequence		Length	Mass (Da)
	Q75WH8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2E2FBA87068472A7	FASTA	430	46,975
	10 20 30 40 50 60 MAEFRIAQDV VARENDRRAS ALKEDYEALG ANLARRGVDI EAVTAKVEKF FVAVPSWGVG 70 80 90 100 110 120 TGGTRFARFP GTGEPRGIFD KLDDCAVIQQ LTRATPNVSL HIPWDKADPK ELKARGDALG 130 140 150 160 170 180 LGFDAMNSNT FSDAPGQAHS YKYGSLSHTD AATRAQAVEH NLECIEIGKA IGSKALTVWI 190 200 210 220 230 240 GDGSNFPGQS NFTRAFERYL SAMAEIYKGL PDDWKLFSEH KMYEPAFYST VVQDWGTNYL 250 260 270 280 290 300 IAQTLGPKAQ CLVDLGHHAP NTNIEMIVAR LIQFGKLGGF HFNDSKYGDD DLDAGAIEPY 310 320 330 340 350 360 RLFLVFNELV DAEARGVKGF HPAHMIDQSH NVTDPIESLI NSANEIRRAY AQALLVDRAA 370 380 390 400 410 420 LSGYQEDNDA LMATETLKRA YRTDVEPILA EARRRTGGAV DPVATYRASG YRARVAAERP 430 ASVAGGGGII

References

[1]	Leang K., Takada G., Ishimura A., Okita M., Izumori K. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The structures of L-rhamnose isomerase from Pseudomonas stutzeri in complexes with L-rhamnose and D-allose provide insights into broad substrate specificity." Yoshida H., Yamada M., Ohyama Y., Takada G., Izumori K., Kamitori S. J. Mol. Biol. 365:1505-1516(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.97 ANGSTROMS) IN COMPLEX WITH ZINC.
[3]	"Catalytic reaction mechanism of Pseudomonas stutzeri L-rhamnose isomerase deduced from X-ray structures." Yoshida H., Yamaji M., Ishii T., Izumori K., Kamitori S. FEBS J. 277:1045-1057(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH COBALT; COPPER; MANGANESE AND ZINC.
[4]	"Elucidation of the role of Ser329 and the C-terminal region in the catalytic activity of Pseudomonas stutzeri L-rhamnose isomerase." Yoshida H., Takeda K., Izumori K., Kamitori S. Protein Eng. Des. Sel. 23:919-927(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) IN COMPLEX WITH MANGANESE.
[5]	"Complex structure of L-rhamnose isomerase with L-rhanmopyranose demonstrates sugar-ring opening mechanism and role of substrate sub-binding site." Yoshida H., Kamitori S. Submitted (AUG-2012) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB121136 Genomic DNA. Translation: BAD14073.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HCV	X-ray	2.00	A/B/C/D	1-430	[»]
2I56	X-ray	1.97	A/B/C/D	1-430	[»]
2I57	X-ray	1.97	A/B/C/D	1-430	[»]
3ITL	X-ray	1.70	A/B/C/D	1-430	[»]
3ITO	X-ray	1.90	A/B/C/D	1-430	[»]
3ITT	X-ray	1.96	A/B/C/D	1-430	[»]
3ITV	X-ray	1.60	A/B/C/D	1-430	[»]
3ITX	X-ray	1.80	A/B/C/D	1-430	[»]
3ITY	X-ray	1.84	A/B/C/D	1-430	[»]
3IUD	X-ray	2.44	A/B/C/D	1-430	[»]
3IUH	X-ray	2.00	A/B/C/D	1-430	[»]
3IUI	X-ray	2.30	A/B/C/D	1-430	[»]
3M0H	X-ray	1.58	A/B/C/D	1-430	[»]
3M0L	X-ray	1.85	A/B/C/D	1-430	[»]
3M0M	X-ray	1.45	A/B/C/D	1-430	[»]
3M0V	X-ray	1.79	A/B/C/D	1-430	[»]
3M0X	X-ray	1.79	A/B/C/D	1-430	[»]
3M0Y	X-ray	1.96	A/B/C/D	1-430	[»]
4GJI	X-ray	1.70	A/B/C/D	1-430	[»]
4GJJ	X-ray	2.38	A/B/C/D	1-430	[»]

ProteinModelPortal

Q75WH8.

SMR

Q75WH8. Positions 3-430.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Enzyme and pathway databases

BRENDA

5.3.1.14. 421.

Family and domain databases

Gene3D

3.20.20.150. 1 hit.

InterPro

IPR013451. L_rhamnose_iso.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]

Pfam

PF01261. AP_endonuc_2. 1 hit.
[Graphical view]

SUPFAM

SSF51658. Xyl_isomerase-like_TIM-brl. 1 hit.

TIGRFAMs

TIGR02629. L_rham_iso_rhiz. 1 hit.

ProtoNet

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Other

EvolutionaryTrace

Q75WH8.

Entry information

Entry name

Q75WH8_PSEST

Accession

Primary (citable) accession number: Q75WH8

Entry history

Integrated into UniProtKB/TrEMBL:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)