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Protein

5-oxoprolinase

Gene

OPLAH

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.

Catalytic activityi

ATP + 5-oxo-L-proline + 2 H2O = ADP + phosphate + L-glutamate.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.5.2.9. 908.

Names & Taxonomyi

Protein namesi
Recommended name:
5-oxoprolinase (EC:3.5.2.9)
Alternative name(s):
5-oxo-L-prolinase
Short name:
5-OPase
Pyroglutamase
Gene namesi
Name:OPLAH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 128812885-oxoprolinasePRO_0000208576Add
BLAST

Proteomic databases

PRIDEiQ75WB5.

Expressioni

Tissue specificityi

Expressed in coronary artery and kidney.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022981.

Structurei

3D structure databases

ProteinModelPortaliQ75WB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the oxoprolinase family.Curated

Phylogenomic databases

eggNOGiCOG0146.
HOGENOMiHOG000047200.
HOVERGENiHBG007870.
InParanoidiQ75WB5.
KOiK01469.

Family and domain databases

InterProiIPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view]
PfamiPF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q75WB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGPEGRFHF AIDRGGTFTD VFAQCPGGHV RVLKLLSEDP ANYVDAPTEG
60 70 80 90 100
IRRILEQEGG VLLPRDRPLD TSRIASIRMG TTVATNALLE QQGERVALLV
110 120 130 140 150
TRGFRDLLHV CTQARAXLFD LAVPMPETLY EEVLEVDERV VLYRGXPGAG
160 170 180 190 200
TPVKGCTGDL LEVQQPVDLG GLRWKLEGLL SRGIRSLAVV LMHSYTWAQH
210 220 230 240 250
EQQVGALARE LGFTHVSLSS EAMPMVRIVP RGHTACADAY LTPTIQRYVQ
260 270 280 290 300
GFRRGFQGQL KDVQVLFMRS DGGLAPMDSF SGSRAVLSGP AGGVVGYSAT
310 320 330 340 350
TYRVEGGQPV IGFDMGGTST DVSRYAGEFE HVFEASTAGV TLQAPQLDIN
360 370 380 390 400
TVAAGGGSRL FFRSGLFVVG PESAGAHPGP ACYRKGGPVT VTDANLVLGR
410 420 430 440 450
LLPASFPCIF GPGEDQPLSP EASRKALEAV ATEVNSFLTN GPCPASPLSL
460 470 480 490 500
EEVAMGFVRV ANEAMCRPIR ALTQARGHDP SAHVLACFGG AGGQHACAIA
510 520 530 540 550
RALGMDTVHI HRHSGLLSAL GLALADVVHE AQEPCSLPYA PETFAQLDQR
560 570 580 590 600
LGRLEEQCVE ALRAQGFPRS QISTESFLHL RYQGTDCALM VSAHQHPASA
610 620 630 640 650
RSPRAGDFGA AFVERYMREF GFIIPERPVV VDDVRVRGTG SSSLRLEDVP
660 670 680 690 700
KAHSGPPRVD KMTQCYFEGG YQETPVYLLG ELGCGHKLQG PCLIIDSNST
710 720 730 740 750
ILVEPGCQAE VTETGDIRIS VGAETASVVG TQLDPIHLTI FSHRFMSIAE
760 770 780 790 800
QMGRILQRTA ISTNIKERLD FSCALFGPDG GLVSNVPHIP VHLGAMQETV
810 820 830 840 850
QFQIQQLGAD LHPGDVLLSN HPSAGGSHLP DLTVITPVFW PGQTRPVFYV
860 870 880 890 900
ASRGHHADIG GITPGSMPPH STSLQQEGAV FLSFKLVHGG VFQEEAVTEA
910 920 930 940 950
LRAPGKIPGC SGTRNLHDNL SDLRAQVAAN QKGIQLVGEL IGQYGLDVVQ
960 970 980 990 1000
AYMGHIQANA ELAVRDMLRA FGTARQARGL PLEVSAEDHM DDGSPIRLRV
1010 1020 1030 1040 1050
QINMSQGSAV FDFSGSGPEV FGNLNAPRAI TLSALIYCLR CLVGRDIPLN
1060 1070 1080 1090 1100
QGCLAPVRVV IPKGSILDPS PDAAVVGGNV LTSQRVVDVI LGAFGACAAS
1110 1120 1130 1140 1150
QGCMNNVTLG NAHMGYYETV AGGAGAGPGW HGRSGVHSHM TNTRITDPEI
1160 1170 1180 1190 1200
LESRYPVILR RFELRLGSGG RGRFRGGDGI IRELLFREEA LLSVLTERRA
1210 1220 1230 1240 1250
FQPYGLMGGE PGARGLNLLI RKDGRTVNLG GKTSVPVYPG DVFCLHTPGG
1260 1270 1280
GGYGDPGDPA PLPGSPLQPL AFPERGSVYE YRRAQEAV
Length:1,288
Mass (Da):137,450
Last modified:July 5, 2004 - v1
Checksum:i61A97ADC5A1A23B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB121737 mRNA. Translation: BAD13433.1.
RefSeqiNP_001001173.1. NM_001001173.1.
UniGeneiBt.3369.

Genome annotation databases

GeneIDi408006.
KEGGibta:408006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB121737 mRNA. Translation: BAD13433.1.
RefSeqiNP_001001173.1. NM_001001173.1.
UniGeneiBt.3369.

3D structure databases

ProteinModelPortaliQ75WB5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022981.

Proteomic databases

PRIDEiQ75WB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi408006.
KEGGibta:408006.

Organism-specific databases

CTDi26873.

Phylogenomic databases

eggNOGiCOG0146.
HOGENOMiHOG000047200.
HOVERGENiHBG007870.
InParanoidiQ75WB5.
KOiK01469.

Enzyme and pathway databases

BRENDAi3.5.2.9. 908.

Miscellaneous databases

NextBioi20818625.

Family and domain databases

InterProiIPR008040. Hydant_A_N.
IPR002821. Hydantoinase_A.
IPR003692. Hydantoinase_B.
[Graphical view]
PfamiPF05378. Hydant_A_N. 1 hit.
PF01968. Hydantoinase_A. 1 hit.
PF02538. Hydantoinase_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Bovine 5-oxo-L-prolinase: simple assay method, purification, cDNA cloning, and detection of mRNA in the coronary artery."
    Watanabe T., Abe K., Ishikawa H., Iijima Y.
    Biol. Pharm. Bull. 27:288-294(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 3-14, TISSUE SPECIFICITY.
    Tissue: Kidney.

Entry informationi

Entry nameiOPLA_BOVIN
AccessioniPrimary (citable) accession number: Q75WB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: July 5, 2004
Last modified: May 27, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.