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Protein

Single-stranded DNA cytosine deaminase

Gene

AICDA

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation (By similarity).By similarity

Catalytic activityi

Cytosine in single-stranded DNA + H2O = uracil in single-stranded DNA + NH3.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Zinc; catalyticBy similarity
Active sitei58 – 581Proton donorBy similarity
Metal bindingi87 – 871Zinc; catalyticBy similarity
Metal bindingi90 – 901Zinc; catalyticBy similarity

GO - Molecular functioni

  1. cytidine deaminase activity Source: UniProtKB
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular response to lipopolysaccharide Source: Ensembl
  2. cytidine deamination Source: UniProtKB
  3. DNA demethylation Source: UniProtKB
  4. isotype switching Source: Ensembl
  5. mRNA processing Source: UniProtKB-KW
  6. negative regulation of methylation-dependent chromatin silencing Source: UniProtKB
  7. somatic hypermutation of immunoglobulin genes Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Single-stranded DNA cytosine deaminase (EC:3.5.4.38)
Alternative name(s):
Activation-induced cytidine deaminase
Cytidine aminohydrolase
Gene namesi
Name:AICDA
Synonyms:AID
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254 Componenti: Unplaced

Subcellular locationi

  1. Nucleus By similarity
  2. Cytoplasm By similarity

  3. Note: Predominantly cytoplasmic but shuttles between the nucleus and the cytoplasm.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. exosome (RNase complex) Source: Ensembl
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198Single-stranded DNA cytosine deaminasePRO_0000171686Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271Phosphothreonine; by PKABy similarity
Modified residuei38 – 381Phosphoserine; by PKABy similarity

Post-translational modificationi

Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity (By similarity).By similarity
Probably monoubiquitinated on several residues by RNF126.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Expressioni

Tissue specificityi

Expressed in thymus, lung, spleen, kidney, small intestine, lymph node and tonsil.1 Publication

Interactioni

Subunit structurei

Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1. Interacts with SUPT6H, TRIM28 and NCL.By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000020480.

Structurei

3D structure databases

ProteinModelPortaliQ75W64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 129107CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 2625Interaction with SUPT6HBy similarityAdd
BLAST
Regioni39 – 424Important for interaction with CTNNBL1By similarity
Regioni88 – 11629Required for interaction with RNF126By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1 – 3030Nuclear localization signalBy similarityAdd
BLAST
Motifi183 – 19816Nuclear export signalBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 CMP/dCMP-type deaminase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG39659.
HOGENOMiHOG000033754.
HOVERGENiHBG050434.
InParanoidiQ75W64.
KOiK10989.
OrthoDBiEOG708W0R.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR013158. APOBEC_N.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF08210. APOBEC_N. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q75W64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSLLMKQRK FLYHFKNVRW AKGRHETYLC YVVKRRDSAT SFSLDFGHLR
60 70 80 90 100
NKSGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVADFLRG
110 120 130 140 150
YPNLSLRIFA ARLYFCEDRK AEPEGLRRLH RAGVQIAIMT FKDYFYCWNT
160 170 180 190
FVENREKTFK AWEGLHENSV RLSRQLRRIL LPLYEVDDLR DAFRTLGL
Length:198
Mass (Da):23,887
Last modified:July 5, 2004 - v1
Checksum:i4BE00D9B9D1550D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB122019 mRNA. Translation: BAD15112.1.
RefSeqiNP_001003380.1. NM_001003380.1.
UniGeneiCfa.4560.

Genome annotation databases

GeneIDi442983.
KEGGicfa:442983.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB122019 mRNA. Translation: BAD15112.1.
RefSeqiNP_001003380.1. NM_001003380.1.
UniGeneiCfa.4560.

3D structure databases

ProteinModelPortaliQ75W64.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000020480.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi442983.
KEGGicfa:442983.

Organism-specific databases

CTDi57379.

Phylogenomic databases

eggNOGiNOG39659.
HOGENOMiHOG000033754.
HOVERGENiHBG050434.
InParanoidiQ75W64.
KOiK10989.
OrthoDBiEOG708W0R.

Miscellaneous databases

NextBioi20831654.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR013158. APOBEC_N.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF08210. APOBEC_N. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of canine activation-induced cytidine deaminase (AID) cDNA and its expression in normal tissues."
    Ohmori K., Maeda S., Okayama T., Masuda K., Ohno K., Tsujimoto H.
    J. Vet. Med. Sci. 66:739-741(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Lymph node.

Entry informationi

Entry nameiAICDA_CANFA
AccessioniPrimary (citable) accession number: Q75W64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: April 1, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.