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Q75W64 (AICDA_CANFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Single-stranded DNA cytosine deaminase

EC=3.5.4.38
Alternative name(s):
Activation-induced cytidine deaminase
Cytidine aminohydrolase
Gene names
Name:AICDA
Synonyms:AID
OrganismCanis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]
Taxonomic identifier9615 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis

Protein attributes

Sequence length198 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation By similarity.

Catalytic activity

Cytosine in single-stranded DNA + H2O = uracil in single-stranded DNA + NH3.

Cofactor

Zinc By similarity.

Subunit structure

Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1 By similarity. Interacts with SUPT6H, TRIM28 and NCL By similarity.

Subcellular location

Nucleus By similarity. Cytoplasm By similarity. Note: Predominantly cytoplasmic but shuttles between the nucleus and the cytoplasm By similarity.

Tissue specificity

Expressed in thymus, lung, spleen, kidney, small intestine, lymph node and tonsil. Ref.1

Post-translational modification

Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity By similarity.

Sequence similarities

Belongs to the cytidine and deoxycytidylate deaminase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 198198Single-stranded DNA cytosine deaminase
PRO_0000171686

Regions

Region1 – 3030Nuclear localization signal By similarity
Region2 – 2625Interaction with SUPT6H By similarity
Region39 – 424Important for interaction with CTNNBL1 By similarity
Region183 – 19816Nuclear export signal By similarity

Sites

Active site581Proton donor By similarity
Metal binding561Zinc; catalytic By similarity
Metal binding871Zinc; catalytic By similarity
Metal binding901Zinc; catalytic By similarity

Amino acid modifications

Modified residue271Phosphothreonine; by PKA By similarity
Modified residue381Phosphoserine; by PKA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q75W64 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 4BE00D9B9D1550D2

FASTA19823,887
        10         20         30         40         50         60 
MDSLLMKQRK FLYHFKNVRW AKGRHETYLC YVVKRRDSAT SFSLDFGHLR NKSGCHVELL 

        70         80         90        100        110        120 
FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVADFLRG YPNLSLRIFA ARLYFCEDRK 

       130        140        150        160        170        180 
AEPEGLRRLH RAGVQIAIMT FKDYFYCWNT FVENREKTFK AWEGLHENSV RLSRQLRRIL 

       190 
LPLYEVDDLR DAFRTLGL 

« Hide

References

[1]"Molecular cloning of canine activation-induced cytidine deaminase (AID) cDNA and its expression in normal tissues."
Ohmori K., Maeda S., Okayama T., Masuda K., Ohno K., Tsujimoto H.
J. Vet. Med. Sci. 66:739-741(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Lymph node.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB122019 mRNA. Translation: BAD15112.1.
RefSeqNP_001003380.1. NM_001003380.1.
UniGeneCfa.4560.

3D structure databases

ProteinModelPortalQ75W64.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9615.ENSCAFP00000020480.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID442983.
KEGGcfa:442983.

Organism-specific databases

CTD57379.

Phylogenomic databases

eggNOGNOG39659.
HOGENOMHOG000033754.
HOVERGENHBG050434.
InParanoidQ75W64.
KOK10989.
OrthoDBEOG708W0R.

Family and domain databases

InterProIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR013158. APOBEC_N.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamPF08210. APOBEC_N. 1 hit.
[Graphical view]
SUPFAMSSF53927. SSF53927. 1 hit.
PROSITEPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20831654.

Entry information

Entry nameAICDA_CANFA
AccessionPrimary (citable) accession number: Q75W64
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families