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Q75W64

- AICDA_CANFA

UniProt

Q75W64 - AICDA_CANFA

Protein

Single-stranded DNA cytosine deaminase

Gene

AICDA

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation By similarity.By similarity

    Catalytic activityi

    Cytosine in single-stranded DNA + H2O = uracil in single-stranded DNA + NH3.

    Cofactori

    Zinc.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi56 – 561Zinc; catalyticBy similarity
    Active sitei58 – 581Proton donorBy similarity
    Metal bindingi87 – 871Zinc; catalyticBy similarity
    Metal bindingi90 – 901Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. cytidine deaminase activity Source: UniProtKB
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cellular response to lipopolysaccharide Source: Ensembl
    2. cytidine deamination Source: UniProtKB
    3. DNA demethylation Source: UniProtKB
    4. isotype switching Source: Ensembl
    5. mRNA processing Source: UniProtKB-KW
    6. negative regulation of methylation-dependent chromatin silencing Source: UniProtKB
    7. somatic hypermutation of immunoglobulin genes Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Single-stranded DNA cytosine deaminase (EC:3.5.4.38)
    Alternative name(s):
    Activation-induced cytidine deaminase
    Cytidine aminohydrolase
    Gene namesi
    Name:AICDA
    Synonyms:AID
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Unplaced

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity
    Note: Predominantly cytoplasmic but shuttles between the nucleus and the cytoplasm.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. exosome (RNase complex) Source: Ensembl
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 198198Single-stranded DNA cytosine deaminasePRO_0000171686Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei27 – 271Phosphothreonine; by PKABy similarity
    Modified residuei38 – 381Phosphoserine; by PKABy similarity

    Post-translational modificationi

    Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Expressed in thymus, lung, spleen, kidney, small intestine, lymph node and tonsil.1 Publication

    Interactioni

    Subunit structurei

    Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1 By similarity. Interacts with SUPT6H, TRIM28 and NCL By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9615.ENSCAFP00000020480.

    Structurei

    3D structure databases

    ProteinModelPortaliQ75W64.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 3030Nuclear localization signalBy similarityAdd
    BLAST
    Regioni2 – 2625Interaction with SUPT6HBy similarityAdd
    BLAST
    Regioni39 – 424Important for interaction with CTNNBL1By similarity
    Regioni183 – 19816Nuclear export signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG39659.
    HOGENOMiHOG000033754.
    HOVERGENiHBG050434.
    InParanoidiQ75W64.
    KOiK10989.
    OrthoDBiEOG708W0R.

    Family and domain databases

    InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR013158. APOBEC_N.
    IPR016193. Cytidine_deaminase-like.
    [Graphical view]
    PfamiPF08210. APOBEC_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53927. SSF53927. 1 hit.
    PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q75W64-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSLLMKQRK FLYHFKNVRW AKGRHETYLC YVVKRRDSAT SFSLDFGHLR    50
    NKSGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVADFLRG 100
    YPNLSLRIFA ARLYFCEDRK AEPEGLRRLH RAGVQIAIMT FKDYFYCWNT 150
    FVENREKTFK AWEGLHENSV RLSRQLRRIL LPLYEVDDLR DAFRTLGL 198
    Length:198
    Mass (Da):23,887
    Last modified:July 5, 2004 - v1
    Checksum:i4BE00D9B9D1550D2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB122019 mRNA. Translation: BAD15112.1.
    RefSeqiNP_001003380.1. NM_001003380.1.
    UniGeneiCfa.4560.

    Genome annotation databases

    GeneIDi442983.
    KEGGicfa:442983.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB122019 mRNA. Translation: BAD15112.1 .
    RefSeqi NP_001003380.1. NM_001003380.1.
    UniGenei Cfa.4560.

    3D structure databases

    ProteinModelPortali Q75W64.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9615.ENSCAFP00000020480.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 442983.
    KEGGi cfa:442983.

    Organism-specific databases

    CTDi 57379.

    Phylogenomic databases

    eggNOGi NOG39659.
    HOGENOMi HOG000033754.
    HOVERGENi HBG050434.
    InParanoidi Q75W64.
    KOi K10989.
    OrthoDBi EOG708W0R.

    Miscellaneous databases

    NextBioi 20831654.

    Family and domain databases

    InterProi IPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR013158. APOBEC_N.
    IPR016193. Cytidine_deaminase-like.
    [Graphical view ]
    Pfami PF08210. APOBEC_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53927. SSF53927. 1 hit.
    PROSITEi PS00903. CYT_DCMP_DEAMINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of canine activation-induced cytidine deaminase (AID) cDNA and its expression in normal tissues."
      Ohmori K., Maeda S., Okayama T., Masuda K., Ohno K., Tsujimoto H.
      J. Vet. Med. Sci. 66:739-741(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Lymph node.

    Entry informationi

    Entry nameiAICDA_CANFA
    AccessioniPrimary (citable) accession number: Q75W64
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3